Rother, Jan

[["dc.bibliographiccitation.journal","European Biophysics Journal"],["dc.bibliographiccitation.volume","40"],["dc.contributor.author","Rother, Jan"],["dc.contributor.author","Pietuch, Anna"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T08:53:37Z"],["dc.date.available","2018-11-07T08:53:37Z"],["dc.date.issued","2011"],["dc.format.extent","231"],["dc.identifier.isi","000293637300661"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22462"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","New york"],["dc.relation.eventlocation","Budapest, HUNGARY"],["dc.relation.issn","0175-7571"],["dc.title","Enhanced stimulation of Toll-like receptor 9 via immunostimulatory nanoparticles"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e80068"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","PLoS One"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Schneider, David"],["dc.contributor.author","Baronsky, Thilo"],["dc.contributor.author","Pietuch, Anna"],["dc.contributor.author","Rother, Jan"],["dc.contributor.author","Oelkers, Marieelen"],["dc.contributor.author","Fichtner, Dagmar"],["dc.contributor.author","Wedlich, Doris"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T09:16:39Z"],["dc.date.available","2018-11-07T09:16:39Z"],["dc.date.issued","2013"],["dc.description.abstract","Structural alterations during epithelial-to-mesenchymal transition (EMT) pose a substantial challenge to the mechanical response of cells and are supposed to be key parameters for an increased malignancy during metastasis. Herein, we report that during EMT, apical tension of the epithelial cell line NMuMG is controlled by cell-cell contacts and the architecture of the underlying actin structures reflecting the mechanistic interplay between cellular structure and mechanics. Using force spectroscopy we find that tension in NMuMG cells slightly increases 24 h after EMT induction, whereas upon reaching the final mesenchymal-like state characterized by a complete loss of intercellular junctions and a concerted down-regulation of the adherens junction protein E-cadherin, the overall tension becomes similar to that of solitary adherent cells and fibroblasts. Interestingly, the contribution of the actin cytoskeleton on apical tension increases significantly upon EMT induction, most likely due to the formation of stable and highly contractile stress fibers which dominate the elastic properties of the cells after the transition. The structural alterations lead to the formation of single, highly motile cells rendering apical tension a good indicator for the cellular state during phenotype switching. In summary, our study paves the way towards a more profound understanding of cellular mechanics governing fundamental morphological programs such as the EMT."],["dc.description.sponsorship","Open-Acces-Publikationsfonds 2013"],["dc.identifier.doi","10.1371/journal.pone.0080068"],["dc.identifier.isi","000328566100009"],["dc.identifier.pmid","24339870"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9505"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27979"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 2.5"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.5"],["dc.title","Tension Monitoring during Epithelial-to-Mesenchymal Transition Links the Switch of Phenotype to Expression of Moesin and Cadherins in NMuMG Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","140046"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Open Biology"],["dc.bibliographiccitation.lastpage","7"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Rother, Jan"],["dc.contributor.author","Nöding, Helen"],["dc.contributor.author","Mey, Ingo"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2019-07-09T11:41:15Z"],["dc.date.available","2019-07-09T11:41:15Z"],["dc.date.issued","2014-05-01"],["dc.description.abstract","Mechanical phenotyping of cells by atomic force microscopy (AFM) was proposed as a novel tool in cancer cell research as cancer cells undergo massive structural changes, comprising remodelling of the cytoskeleton and changes of their adhesive properties. In this work, we focused on the mechanical properties of human breast cell lines with different metastatic potential by AFM-based microrheology experiments. Using this technique, we are not only able to quantify the mechanical properties of living cells in the context of malignancy, but we also obtain a descriptor, namely the loss tangent, which provides model-independent information about the metastatic potential of the cell line. Including also other cell lines from different organs shows that the loss tangent (G″/G') increases generally with the metastatic potential from MCF-10A representing benign cells to highly malignant MDA-MB-231 cells."],["dc.identifier.doi","10.1098/rsob.140046"],["dc.identifier.fs","609535"],["dc.identifier.pmid","24850913"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11878"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58382"],["dc.language.iso","en"],["dc.relation.issn","2046-2441"],["dc.rights.access","openAccess"],["dc.subject.mesh","Animals"],["dc.subject.mesh","Breast Neoplasms"],["dc.subject.mesh","Cell Line"],["dc.subject.mesh","Dogs"],["dc.subject.mesh","Elasticity"],["dc.subject.mesh","Female"],["dc.subject.mesh","Humans"],["dc.subject.mesh","MCF-7 Cells"],["dc.subject.mesh","Mice"],["dc.subject.mesh","Microscopy, Atomic Force"],["dc.subject.mesh","Models, Biological"],["dc.subject.mesh","NIH 3T3 Cells"],["dc.subject.mesh","Neoplasm Metastasis"],["dc.title","Atomic force microscopy-based microrheology reveals significant differences in the viscoelastic response between malign and benign cell lines."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","20141057"],["dc.bibliographiccitation.issue","103"],["dc.bibliographiccitation.journal","Journal of the Royal Society Interface"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Rother, Jan"],["dc.contributor.author","Buechsenschuetz-Goebeler, Matthias"],["dc.contributor.author","Noeding, Helen"],["dc.contributor.author","Steltenkamp, Siegfried"],["dc.contributor.author","Samwer, Konrad H."],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T10:01:02Z"],["dc.date.available","2018-11-07T10:01:02Z"],["dc.date.issued","2015"],["dc.description.abstract","The impact of substrate topographyon the morphological and mechanical properties of confluent MDCK-II cells cultured on porous substrates was scrutinized by means of various imaging techniques as well as atomic force microscopy comprising force volume and microrheology measurements. Regardless of the pore size, ranging from 450 to 5500 nm in diameter, cells were able to span the pores. They did not crawl into the holes or grow around the pores. Generally, we found that cells cultured on non-porous surfaces are stiffer, i.e. cortical tension rises from 0.1 to 0.3 mN m(-1), and less fluid than cells grown over pores. The mechanical data are corroborated by electron microscopy imaging showing more cytoskeletal filaments on flat samples in comparison to porous ones. By contrast, cellular compliance increases with pore size and cells display a more fluid-like behaviour on larger pores. Interestingly, cells on pores larger than 3500 nm produce thick actin bundles that bridge the pores and thereby strengthen the contact zone of the cells."],["dc.description.sponsorship","DFG [CRC 937]"],["dc.identifier.doi","10.1098/rsif.2014.1057"],["dc.identifier.isi","000353394100006"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/37932"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Royal Soc"],["dc.relation.issn","1742-5662"],["dc.relation.issn","1742-5689"],["dc.title","Cytoskeleton remodelling of confluent epithelial cells cultured on porous substrates"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","254"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Nanotoxicology"],["dc.bibliographiccitation.lastpage","268"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Tarantola, Marco"],["dc.contributor.author","Pietuch, Anna"],["dc.contributor.author","Schneider, David"],["dc.contributor.author","Rother, Jan"],["dc.contributor.author","Sunnick, Eva"],["dc.contributor.author","Rosman, Christina"],["dc.contributor.author","Pierrat, Sebastien"],["dc.contributor.author","Soennichsen, Carsten"],["dc.contributor.author","Wegener, Joachim"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T08:55:39Z"],["dc.date.available","2018-11-07T08:55:39Z"],["dc.date.issued","2011"],["dc.description.abstract","Nanoparticle exposure is monitored by a combination of two label-free and non-invasive biosensor devices which detect cellular shape and viscoelasticity (quartz crystal microbalance), cell motility and the dynamics of epithelial cell-cell contacts (electric cell-substrate impedance sensing). With these tools we have studied the impact of nanoparticle shape on cellular physiology. Gold (Au) nanoparticles coated with CTAB were synthesized and studied in two distinct shapes: Spheres with a diameter of (43 +/-+/- 4) nm and rods with a size of (38 +/-+/- 7) nm xx (17 +/-+/- 3) nm. Dose-response experiments were accompanied by conventional cytotoxicity tests as well as fluorescence and dark-field microscopy to visualize the intracellular particle distribution. We found that spherical gold nanoparticles with identical surface functionalization are generally more toxic and more efficiently ingested than rod-shaped particles. We largely attribute the higher toxicity of CTAB-coated spheres as compared to rod-shaped particles to a higher release of toxic CTAB upon intracellular aggregation."],["dc.description.sponsorship","German Science Foundation (DFG) [JA 963/10-1]"],["dc.identifier.doi","10.3109/17435390.2010.528847"],["dc.identifier.isi","000290936000013"],["dc.identifier.pmid","21050076"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22954"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Taylor & Francis Ltd"],["dc.relation.issn","1743-5404"],["dc.relation.issn","1743-5390"],["dc.title","Toxicity of gold-nanoparticles: Synergistic effects of shape and surface functionalization on micromotility of epithelial cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","150038"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Open Biology"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Rother, Jan"],["dc.contributor.author","Richter, C."],["dc.contributor.author","Turco, L."],["dc.contributor.author","Knoch, F."],["dc.contributor.author","Mey, Ingo"],["dc.contributor.author","Luther, Stefan"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Bodenschatz, Eberhard"],["dc.contributor.author","Tarantola, Marco"],["dc.date.accessioned","2018-11-07T09:56:14Z"],["dc.date.available","2018-11-07T09:56:14Z"],["dc.date.issued","2015"],["dc.description.abstract","Electromechanical function of cardiacmuscle depends critically on the crosstalk of myocytes with non-myocytes. Upon cardiac fibrosis, fibroblasts translocate into infarcted necrotic tissue and alter their communication capabilities. In the present in vitro study, we determined a multiple parameter space relevant for fibrotic cardiac tissue development comprising the following essential processes: (i) adhesion to substrates with varying elasticity, (ii) dynamics of contractile function, and (iii) electromechanical connectivity. By combining electric cell-substrate impedance sensing (ECIS) with conventional optical microscopy, we could measure the impact of fibroblast-cardiomyocyte ratio on the aforementioned parameters in a non-invasive fashion. Adhesion to electrodes was quantified via spreading rates derived from impedance changes, period analysis allowed us to measure contraction dynamics and modulations of the barrier resistance served as a measure of connectivity. In summary, we claim that: (i) a preferred window for substrate elasticity around 7 kPa for low fibroblast content exists, which is shifted to stiffer substrates with increasing fibroblast fractions. (ii) Beat frequency decreases nonlinearly with increasing fraction of fibroblasts, while (iii) the intercellular resistance increases with a maximal functional connectivity at 75% fibroblasts. For the first time, cardiac cell-cell junction density-dependent connectivity in co-cultures of cardiomyocytes and fibroblasts was quantified using ECIS."],["dc.identifier.doi","10.1098/rsob.150038"],["dc.identifier.fs","613600"],["dc.identifier.isi","000358860700004"],["dc.identifier.pmid","26085516"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12072"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36916"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/119"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation","info:eu-repo/grantAgreement/EC/FP7/241526/EU//EUTRIGTREAT"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | C03: Erholung nach Herzinsuffizienz: Analyse der transmuralen mechano-elektrischen Funktionsstörung"],["dc.relation.issn","2046-2441"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Bodenschatz (Laboratory for Fluid Physics, Pattern Formation and Biocomplexity)"],["dc.relation.workinggroup","RG Luther (Biomedical Physics)"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Crosstalk of cardiomyocytes and fibroblasts in co-cultures"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","124"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Nature Photonics"],["dc.bibliographiccitation.lastpage","127"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Chizhik, Alexey I."],["dc.contributor.author","Rother, Jan"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2018-04-23T11:49:27Z"],["dc.date.available","2018-04-23T11:49:27Z"],["dc.date.issued","2014"],["dc.description.abstract","The discovery of Förster resonance energy transfer (FRET)1 has revolutionized our ability to measure inter- and intramolecular distances on the nanometre scale using fluorescence imaging. The phenomenon is based on electromagnetic-field-mediated energy transfer from an optically excited donor to an acceptor. We replace the acceptor molecule with a metallic film and use the measured energy transfer efficiency from donor molecules to metal surface plasmons2 to accurately deduce the distance between the molecules and metal. Like FRET, this makes it possible to localize emitters with nanometre accuracy, but the distance range over which efficient energy transfer takes place is an order of magnitude larger than for conventional FRET. This creates a new way to localize fluorescent entities on a molecular scale, over a distance range of more than 100 nm. We demonstrate the power of this method by profiling the basal lipid membrane of living cells."],["dc.identifier.doi","10.1038/nphoton.2013.345"],["dc.identifier.gro","3142121"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13701"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.status","final"],["dc.relation.doi","10.1038/nphoton.2013.345"],["dc.relation.issn","1749-4885"],["dc.title","Metal-induced energy transfer for live cell nanoscopy"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","14700"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Brueckner, Bastian Rouven"],["dc.contributor.author","Pietuch, Anna"],["dc.contributor.author","Nehls, Stefan"],["dc.contributor.author","Rother, Jan"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T09:50:22Z"],["dc.date.available","2018-11-07T09:50:22Z"],["dc.date.issued","2015"],["dc.description.abstract","Plasma membrane tension is responsible for a variety of cellular functions such as motility, cell division, and endocytosis. Since membrane tension is dominated by the attachment of the actin cortex to the inner leaflet of the plasma membrane, we investigated the importance of ezrin, a major cross-linker of the membrane-cytoskeleton interface, for cellular mechanics of confluent MDCK II cells. For this purpose, we carried out ezrin depletion experiments and also enhanced the number of active ezrin molecules at the interface. Mechanical properties were assessed by force indentation experiments followed by membrane tether extraction. PIP2 micelles were injected into individual living cells to reinforce the linkage between plasma membrane and actin-cortex, while weakening of this connection was reached by ezrin siRNA and administration of the inhibitors neomycin and NSC 668394, respectively. We observed substantial stiffening of cells and an increase in membrane tension after addition of PIP2 micelles. In contrast, reduction of active ezrin led to a decrease of membrane tension accompanied by loss of excess surface area, increase in cortical tension, remodelling of actin cytoskeleton, and reduction of cell height. The data confirm the importance of the ezrin-mediated connection between plasma membrane and cortex for cellular mechanics and cell morphology."],["dc.description.sponsorship","Open-Access Publikationsfonds 2015"],["dc.identifier.doi","10.1038/srep14700"],["dc.identifier.isi","000362173300001"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12274"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/35692"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","2045-2322"],["dc.rights.access","openAccess"],["dc.title","Ezrin is a Major Regulator of Membrane Tension in Epithelial Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]