Reddy, Jithender G.

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.journal","Journal of Biomolecular NMR"],["dc.bibliographiccitation.lastpage","9"],["dc.contributor.author","Reddy, Jithender G."],["dc.contributor.author","Pratihar, Supriya"],["dc.contributor.author","Frischkorn, Sebastian"],["dc.contributor.author","Becker, Stefan"],["dc.contributor.author","Griesinger, Christian"],["dc.contributor.author","Lee, Donghan"],["dc.date.accessioned","2018-01-17T11:30:39Z"],["dc.date.available","2018-01-17T11:30:39Z"],["dc.date.issued","2017"],["dc.description.abstract","Molecular dynamics play a significant role in how molecules perform their function. A critical method that provides information on dynamics, at the atomic level, is NMR-based relaxation dispersion (RD) experiments. RD experiments have been utilized for understanding multiple biological processes occurring at micro-to-millisecond time, such as enzyme catalysis, molecular recognition, ligand binding and protein folding. Here, we applied the recently developed high-power RD concept to the Carr-Purcell-Meiboom-Gill sequence (extreme CPMG; E-CPMG) for the simultaneous detection of fast and slow dynamics. Using a fast folding protein, gpW, we have shown that previously inaccessible kinetics can be accessed with the improved precision and efficiency of the measurement by using this experiment."],["dc.identifier.doi","10.1007/s10858-017-0155-0"],["dc.identifier.pmid","29188417"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11678"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1573-5001"],["dc.title","Simultaneous determination of fast and slow dynamics in molecules using extreme CPMG relaxation dispersion experiments"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","65"],["dc.bibliographiccitation.journal","Journal of Magnetic Resonance (1969)"],["dc.bibliographiccitation.lastpage","69"],["dc.bibliographiccitation.volume","269"],["dc.contributor.author","Chakrabarti, Kalyan S."],["dc.contributor.author","Ban, David"],["dc.contributor.author","Pratihar, Supriya"],["dc.contributor.author","Reddy, Jithender G."],["dc.contributor.author","Becker, Stefan"],["dc.contributor.author","Griesinger, Christian"],["dc.contributor.author","Lee, Donghan"],["dc.date.accessioned","2017-09-07T11:44:45Z"],["dc.date.available","2017-09-07T11:44:45Z"],["dc.date.issued","2016"],["dc.description.abstract","Exchange-mediated saturation transfer (EST) provides critical information regarding dynamics of molecules. In typical applications EST is studied by either scanning a wide range of N-15 chemical shift offsets where the applied N-15 irradiation field strength is on the order of hundreds of Hertz or, scanning a narrow range of N-15 chemical shift offsets where the applied N-15 irradiation field-strength is on the order of tens of Hertz during the EST period. The H-1 decoupling during the EST delay is critical as incomplete decoupling causes broadening of the EST profile, which could possibly result in inaccuracies of the extracted kinetic parameters and transverse relaxation rates. Currently two different H-1 decoupling schemes have been employed, intermittently applied 180 pulses and composite-pulse-decoupling (CPD), for situations where a wide range, or narrow range of N-15 chemical shift offsets are scanned, respectively. We show that high-power CPD provides artifact free EST experiments, which can be universally implemented regardless of the offset range or irradiation field-strengths. (C) 2016 Elsevier Inc. All rights reserved."],["dc.identifier.doi","10.1016/j.jmr.2016.05.013"],["dc.identifier.gro","3141641"],["dc.identifier.isi","000381239900007"],["dc.identifier.pmid","27240144"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/4344"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Max Planck Society; EU (ERC) [233227]; James Graham Brown Foundation"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Academic Press Inc Elsevier Science"],["dc.relation.eissn","1096-0856"],["dc.relation.issn","1090-7807"],["dc.title","High-power H-1 composite pulse decoupling provides artifact free exchange-mediated saturation transfer (EST) experiments"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]