Reversibel schaltbare fluoreszierende Proteine für die Superauflösung

ISSN
0947-0867
1868-6249
Date Issued
2016
Author(s)
DOI
10.1007/s12268-016-0699-7
Abstract
Super-resolution microscopy, or nanoscopy, enables the visualization of cellular structures inaccessible to conventional light microscopy. RESOLFT nanoscopy is especially suitable for the imaging of living cells. It requires fluorescent proteins (RSFPs) that can be repeatedly switched between a fluorescent and a non-fluorescent state by light. We have analyzed the molecular switching mechanisms and generated a family of RSFPs specifically tailored for live cell RESOLFT nanoscopy.