Development of electrochemiluminescence-based singleplex and multiplex assays for the quantification of alpha-synuclein and other proteins in cerebrospinal fluid

The need for improved diagnostic accuracy and markers of progression in neurodegenerative diseases motivates the identification of objective biomarkers as well as optimized assays for their quantification. Several potential marker candidates for Parkinson's disease (PD) in cerebrospinal fluid have been identified. These include alpha-synuclein, a major constituent of the intracellular aggregates. We give a general overview and details of our experience in converting established enzyme-linked immunoabsorbent assays (for alpha-synuclein and other proteins) onto an electrochemiluminescence-based platform as well as considerations on multiplexing different assays for PD. (c) 2012 Published by Elsevier Inc.