Dual-Label STED Nanoscopy of Living Cells Using Photochromism

ISSN
1530-6984
Date Issued
2011
Author(s)
DOI
10.1021/nl202290w
Abstract
We demonstrate live-cell STED microscopy of two protein species using photochromic green fluorescent proteins as markers. The reversible photoswitching of two markers is implemented so that they can be discerned with a single excitation and STED wavelength and a single detection channel. Dual-label STED microscopy is shown in living mammalian cells.