Photoswitching of the Fluorescent Protein asFP595: Mechanism, Proton Pathways, and Absorption Spectra

ISSN
1433-7851
Date Issued
2007
Author(s)
Klingen, Astrid R.
Ullmann, G. Matthias
Boggio-Pasqua, Martial
Robb, Michael A.
DOI
10.1002/ange.200602315
Abstract
Molecular light‐switch: Off–on switching of the fluorescence of the protein asFP595 involves a trans–cis isomerization. Mixed quantum/classical simulations elucidate the spectroscopic properties of asFP595 and give detailed insights into the photoswitching mechanism. The conformational trans–cis switching triggers a proton‐transfer cascade between the chromophore and adjacent amino acids.