Genome-wide SWAp-Tag yeast libraries for proteome exploration

ISSN
1548-7091
Date Issued
2018
Author(s)
Weill, Uri
Yofe, Ido
Sass, Ehud
Stynen, Bram
Davidi, Dan
Natarajan, Janani
Ben-Menachem, Reut
Avihou, Zohar
Goldman, Omer
Harpaz, Nofar
Chuartzman, Silvia
Kniazev, Kiril
Knoblach, Barbara
Laborenz, Janina
Boos, Felix
Kowarzyk, Jacqueline
Ben-Dor, Shifra
Zalckvar, Einat
Herrmann, Johannes M.
Rachubinski, Richard A.
Pines, Ophry
Rapaport, Doron
Michnick, Stephen W.
Levy, Emmanuel D.
DOI
10.1038/s41592-018-0044-9
Abstract
Yeast libraries revolutionized the systematic study of cell biology. To extensively increase the number of such libraries, we used our previously devised SWAp-Tag (SWAT) approach to construct a genome-wide library of ~5,500 strains carrying the SWAT NOP1promoter-GFP module at the N terminus of proteins. In addition, we created six diverse libraries that restored the native regulation, created an overexpression library with a Cherry tag, or enabled protein complementation assays from two fragments of an enzyme or fluorophore. We developed methods utilizing these SWAT collections to systematically characterize the yeast proteome for protein abundance, localization, topology, and interactions.