Fluorogenic probes for live-cell imaging of the cytoskeleton

ISSN
1548-7091
Date Issued
2014
Author(s)
Lukinavicius, Grazvydas
Reymond, Luc
Masharina, Anastasiya
Güther, Angelika
Fournier, Mathias
Rizzo, Stefano
Waldmann, Herbert
Blaukopf, Claudia
Sommer, Christoph
Gerlich, Daniel Wolfram
Arndt, Hans-Dieter
Johnsson, Kai
DOI
10.1038/NMETH.2972
Abstract
We introduce far-red, fluorogenic probes that combine minimal cytotoxicity with excellent brightness and photostability for fluorescence imaging of actin and tubulin in living cells. Applied in stimulated emission depletion (STED) microscopy, they reveal the ninefold symmetry of the centrosome and the spatial organization of actin in the axon of cultured rat neurons with a resolution unprecedented for imaging cytoskeletal structures in living cells.