Kinetics of Conformational Sampling in Ubiquitin

ISSN
1433-7851
Date Issued
2011
Author(s)
Funk, Michael
Gulich, Rudolf
Egger, Dalia
Walter, Korvin F. A.
Fenwick, R. Bryn
Pichierri, Fabio
Lange, Oliver F.
Salvatella, Xavier
Wolf, Martin
Loidl, Alois
Lunkenheimer, Peter
DOI
10.1002/anie.201105086
Abstract
The lifetime of interconversion among the ground states of a protein ensemble representation could only be assigned to a time window that is four orders of magnitude large and ranges from 4 ns to 50 μs. By combining temperature-dependent NMR relaxation dispersion (RD) experiments and dielectric relaxation (DR) spectroscopy in solution, the lifetime was now identified to a value of (10±9) μs at 309 K.