Wetzel, Daniela

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","BMC Genomics"],["dc.bibliographiccitation.lastpage","14"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Brzuszkiewicz, Elzbieta B."],["dc.contributor.author","Gunka, Katrin"],["dc.contributor.author","Starke, Jessica"],["dc.contributor.author","Riedel, Thomas"],["dc.contributor.author","Bunk, Boyke"],["dc.contributor.author","Spröer, Cathrin"],["dc.contributor.author","Wetzel, Daniela"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Chibani, Cynthia"],["dc.contributor.author","Bohne, Wolfgang"],["dc.contributor.author","Overmann, Jörg"],["dc.contributor.author","Zimmermann, Ortrud"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Liesegang, Heiko"],["dc.date.accessioned","2019-07-09T11:45:11Z"],["dc.date.available","2019-07-09T11:45:11Z"],["dc.date.issued","2018"],["dc.description.abstract","BACKGROUND: Clostridioides difficile infections (CDI) have emerged over the past decade causing symptoms that range from mild, antibiotic-associated diarrhea (AAD) to life-threatening toxic megacolon. In this study, we describe a multiple and isochronal (mixed) CDI caused by the isolates DSM 27638, DSM 27639 and DSM 27640 that already initially showed different morphotypes on solid media. RESULTS: The three isolates belonging to the ribotypes (RT) 012 (DSM 27639) and 027 (DSM 27638 and DSM 27640) were phenotypically characterized and high quality closed genome sequences were generated. The genomes were compared with seven reference strains including three strains of the RT 027, two of the RT 017, and one of the RT 078 as well as a multi-resistant RT 012 strain. The analysis of horizontal gene transfer events revealed gene acquisition incidents that sort the strains within the time line of the spread of their RTs within Germany. We could show as well that horizontal gene transfer between the members of different RTs occurred within this multiple infection. In addition, acquisition and exchange of virulence-related features including antibiotic resistance genes were observed. Analysis of the two genomes assigned to RT 027 revealed three single nucleotide polymorphisms (SNPs) and apparently a regional genome modification within the flagellar switch that regulates the fli operon. CONCLUSION: Our findings show that (i) evolutionary events based on horizontal gene transfer occur within an ongoing CDI and contribute to the adaptation of the species by the introduction of new genes into the genomes, (ii) within a multiple infection of a single patient the exchange of genetic material was responsible for a much higher genome variation than the observed SNPs."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2018"],["dc.identifier.doi","10.1186/s12864-017-4368-0"],["dc.identifier.pmid","29291715"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15054"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59178"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","In goescholar not merged with http://resolver.sub.uni-goettingen.de/purl?gs-1/15123 but duplicate"],["dc.notes.status","final"],["dc.relation.issn","1471-2164"],["dc.rights","CC BY 4.0"],["dc.rights.access","openAccess"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","570"],["dc.title","Comparative genome and phenotypic analysis of three Clostridioides difficile strains isolated from a single patient provide insight into multiple infection of C. difficile."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","652"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","International Journal of Medical Microbiology"],["dc.bibliographiccitation.lastpage","656"],["dc.bibliographiccitation.volume","306"],["dc.contributor.author","Janssen, Iryna"],["dc.contributor.author","Cooper, Paul"],["dc.contributor.author","Gunka, Katrin"],["dc.contributor.author","Rupnik, Maja"],["dc.contributor.author","Wetzel, Daniela"],["dc.contributor.author","Zimmermann, Ortrud"],["dc.contributor.author","Gross, Uwe"],["dc.date.accessioned","2018-11-07T10:05:02Z"],["dc.date.available","2018-11-07T10:05:02Z"],["dc.date.issued","2016"],["dc.description.abstract","Since data about Clostridium difficile infection in sub-Saharan Africa are scarce, we determined its epidemiology and risk factors in a cross-sectional study in Eikwe, a rural community in Ghana. We tested stool samples from 176 hospitalized patients with diarrhoea and from 131 asymptomatic non-hospitalized individuals for C difficile and some other enteric pathogens. The overall prevalence rate of C difficile was 4.9% with ribotype 084 being predominant. With 75% of the isolates, a high rate of nontoxigenic strains was present in symptomatic patients, most of whom had no other identified enteric pathogens. All strains were susceptible against metronidazole and vancomycin, respectively. Data on lifestyle and medical history showed that age <5 years (p=0.004), and use of ceftriaxone (p =0.023) were the most important risk factors for C difficile carriage status. Although our data suggest that C. difficile is currently not a major cause of diarrhoea in this setting, the epidemiology of C difficile in sub-Saharan Africa awaits further investigation. (C) 2016 Elsevier GmbH. All rights reserved."],["dc.description.sponsorship","Federal State of Lower Saxony, Niedersachsisches Vorab [VWZN2889]"],["dc.identifier.doi","10.1016/j.ijmm.2016.09.004"],["dc.identifier.isi","000390824600007"],["dc.identifier.pmid","27693000"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38815"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Gmbh, Urban & Fischer Verlag"],["dc.relation.issn","1618-0607"],["dc.relation.issn","1438-4221"],["dc.title","High prevalence of nontoxigenic Clostridium difficile isolated from hospitalized and non-hospitalized individuals in rural Ghana"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","311"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","International Journal of Medical Microbiology"],["dc.bibliographiccitation.lastpage","320"],["dc.bibliographiccitation.volume","307"],["dc.contributor.author","Riedel, Thomas"],["dc.contributor.author","Wetzel, Daniela"],["dc.contributor.author","Hofmann, Julia Danielle"],["dc.contributor.author","Plorin, Simon Paul Erich Otto"],["dc.contributor.author","Dannheim, Henning"],["dc.contributor.author","Berges, Mareike"],["dc.contributor.author","Zimmermann, Ortrud"],["dc.contributor.author","Bunk, Boyke"],["dc.contributor.author","Schober, Isabel"],["dc.contributor.author","Spröer, Cathrin"],["dc.contributor.author","Liesegang, Heiko"],["dc.contributor.author","Jahn, Dieter"],["dc.contributor.author","Overmann, Jörg"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Neumann-Schaal, Meina"],["dc.date.accessioned","2020-12-10T14:24:37Z"],["dc.date.available","2020-12-10T14:24:37Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1016/j.ijmm.2017.05.007"],["dc.identifier.issn","1438-4221"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/72302"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","High metabolic versatility of different toxigenic and non-toxigenic Clostridioides difficile isolates"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]