Grabner, Chad Paul

[["dc.contributor.author","Grabner, Chad Paul"],["dc.contributor.author","Moser, Tobias"],["dc.date.accessioned","2021-04-29T08:36:25Z"],["dc.date.available","2021-04-29T08:36:25Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1101/2020.10.12.336503"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/84558"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/285"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.workinggroup","RG Moser (Molecular Anatomy, Physiology and Pathology of Sound Encoding)"],["dc.title","The mammalian rod synaptic ribbon is essential for Cav channel facilitation and ultrafast fusion of the readily releasable pool of vesicles"],["dc.type","preprint"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","eLife"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Grabner, Chad Paul"],["dc.contributor.author","Moser, Tobias"],["dc.date.accessioned","2021-12-01T09:22:56Z"],["dc.date.available","2021-12-01T09:22:56Z"],["dc.date.issued","2021"],["dc.description.abstract","Rod photoreceptors (PRs) use ribbon synapses to transmit visual information. To signal ‘no light detected’ they release glutamate continually to activate post-synaptic receptors. When light is detected glutamate release pauses. How a rod’s individual ribbon enables this process was studied here by recording evoked changes in whole-cell membrane capacitance from wild-type and ribbonless ( Ribeye -ko) mice. Wild-type rods filled with high (10 mM) or low (0.5 mM) concentrations of the Ca 2+ -buffer EGTA created a readily releasable pool (RRP) of 87 synaptic vesicles (SVs) that emptied as a single kinetic phase with a τ<0.4 ms. The lower concentration of EGTA accelerated Ca v channel opening and facilitated release kinetics. In contrast, ribbonless rods created a much smaller RRP of 22 SVs, and they lacked Ca v channel facilitation; however, Ca 2+ channel-release coupling remained tight. These release deficits caused a sharp attenuation of rod-driven scotopic light responses. We conclude that the synaptic ribbon facilitates Ca 2+ -influx and establishes a large RRP of SVs."],["dc.identifier.doi","10.7554/eLife.63844"],["dc.identifier.pmid","34617508"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/94521"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/386"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/138"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-478"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation","SFB 1286 | B05: Quantitative molekulare Physiologie aktiver Zonen in Calyx-Synapsen"],["dc.relation.eissn","2050-084X"],["dc.relation.workinggroup","RG Moser (Molecular Anatomy, Physiology and Pathology of Sound Encoding)"],["dc.rights","CC BY 4.0"],["dc.title","The mammalian rod synaptic ribbon is essential for Cav channel facilitation and ultrafast synaptic vesicle fusion"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1123"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","The Journal of Cell Biology"],["dc.bibliographiccitation.lastpage","1140"],["dc.bibliographiccitation.volume","204"],["dc.contributor.author","Dhara, Madhurima"],["dc.contributor.author","Yarzagaray, Antonio"],["dc.contributor.author","Schwarz, Yvonne"],["dc.contributor.author","Dutta, Soumyajit"],["dc.contributor.author","Grabner, Chad"],["dc.contributor.author","Moghadam, Paanteha K."],["dc.contributor.author","Bost, Anneka"],["dc.contributor.author","Schirra, Claudia"],["dc.contributor.author","Rettig, Jens"],["dc.contributor.author","Reim, Kerstin"],["dc.contributor.author","Brose, Nils"],["dc.contributor.author","Mohrmann, Ralf"],["dc.contributor.author","Bruns, Dieter"],["dc.date.accessioned","2017-09-07T11:46:24Z"],["dc.date.available","2017-09-07T11:46:24Z"],["dc.date.issued","2014"],["dc.description.abstract","ComplexinII (CpxII) and SynaptotagminI (SytI) have been implicated in regulating the function of SNARE proteins in exocytosis, but their precise mode of action and potential interplay have remained unknown. In this paper, we show that CpxII increases Ca2+-triggered vesicle exocytosis and accelerates its secretory rates, providing two independent, but synergistic, functions to enhance synchronous secretion. Specifically, we demonstrate that the C-terminal domain of CpxII increases the pool of primed vesicles by hindering premature exocytosis at submicromolar Ca2+ concentrations, whereas the N-terminal domain shortens the secretory delay and accelerates the kinetics of Ca2+-triggered exocytosis by increasing the Ca2+ affinity of synchronous secretion. With its C terminus, CpxII attenuates fluctuations of the early fusion pore and slows its expansion but is functionally antagonized by SytI, enabling rapid transmitter discharge from single vesicles. Thus, our results illustrate how key features of CpxII, SytI, and their interplay transform the constitutively active SNARE-mediated fusion mechanism into a highly synchronized, Ca2+-triggered release apparatus."],["dc.identifier.doi","10.1083/jcb.201311085"],["dc.identifier.gro","3142163"],["dc.identifier.isi","000333903600007"],["dc.identifier.pmid","24687280"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/5233"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1540-8140"],["dc.relation.issn","0021-9525"],["dc.title","Complexin synchronizes primed vesicle exocytosis and regulates fusion pore dynamics"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","eLife"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Jean, Philippe"],["dc.contributor.author","Lopez de la Morena, David"],["dc.contributor.author","Michanski, Susann"],["dc.contributor.author","Jaime Tobón, Lina María"],["dc.contributor.author","Gültas, Mehmet"],["dc.contributor.author","Maxeiner, Stephan"],["dc.contributor.author","Strenzke, Nicola"],["dc.contributor.author","Chakrabarti, Rituparna"],["dc.contributor.author","Picher, Maria Magdalena"],["dc.contributor.author","Neef, Jakob"],["dc.contributor.author","Jung, SangYong"],["dc.contributor.author","Neef, Andreas"],["dc.contributor.author","Wichmann, Carolin"],["dc.contributor.author","Grabner, Chad"],["dc.contributor.author","Moser, Tobias"],["dc.date.accessioned","2020-11-24T10:41:13Z"],["dc.date.available","2020-11-24T10:41:13Z"],["dc.date.issued","2018"],["dc.description.abstract","We studied the role of the synaptic ribbon for sound encoding at the synapses between inner hair cells (IHCs) and spiral ganglion neurons (SGNs) in mice lacking RIBEYE (RBEKO/KO). Electron and immunofluorescence microscopy revealed a lack of synaptic ribbons and an assembly of several small active zones (AZs) at each synaptic contact. Spontaneous and sound-evoked firing rates of SGNs and their compound action potential were reduced, indicating impaired transmission at ribbonless IHC-SGN synapses. The temporal precision of sound encoding was impaired and the recovery of SGN-firing from adaptation indicated slowed synaptic vesicle (SV) replenishment. Activation of Ca2+-channels was shifted to more depolarized potentials and exocytosis was reduced for weak depolarizations. Presynaptic Ca2+-signals showed a broader spread, compatible with the altered Ca2+-channel clustering observed by super-resolution immunofluorescence microscopy. We postulate that RIBEYE disruption is partially compensated by multi-AZ organization. The remaining synaptic deficit indicates ribbon function in SV-replenishment and Ca2+-channel regulation."],["dc.identifier.doi","10.7554/eLife.29275"],["dc.identifier.eissn","2050-084X"],["dc.identifier.pmid","29328020"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/69157"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation.issn","2050-084X"],["dc.title","The synaptic ribbon is critical for sound encoding at high rates and with temporal precision"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

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[["dc.bibliographiccitation.firstpage","3993"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","The Journal of Cell Biology"],["dc.bibliographiccitation.lastpage","4006"],["dc.bibliographiccitation.volume","217"],["dc.contributor.author","Hagiwara, Akari"],["dc.contributor.author","Kitahara, Yosuke"],["dc.contributor.author","Grabner, Chad Paul"],["dc.contributor.author","Vogl, Christian"],["dc.contributor.author","Abe, Manabu"],["dc.contributor.author","Kitta, Ryo"],["dc.contributor.author","Ohta, Keisuke"],["dc.contributor.author","Nakamura, Keiichiro"],["dc.contributor.author","Sakimura, Kenji"],["dc.contributor.author","Moser, Tobias"],["dc.contributor.author","Nishi, Akinori"],["dc.contributor.author","Ohtsuka, Toshihisa"],["dc.date.accessioned","2020-12-10T18:15:36Z"],["dc.date.available","2020-12-10T18:15:36Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1083/jcb.201704076"],["dc.identifier.eissn","1540-8140"],["dc.identifier.issn","0021-9525"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/74896"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Cytomatrix proteins CAST and ELKS regulate retinal photoreceptor development and maintenance"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","103"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Physiological Reviews"],["dc.bibliographiccitation.lastpage","144"],["dc.bibliographiccitation.volume","100"],["dc.contributor.author","Moser, Tobias"],["dc.contributor.author","Grabner, Chad P."],["dc.contributor.author","Schmitz, Frank"],["dc.date.accessioned","2020-12-10T18:37:40Z"],["dc.date.available","2020-12-10T18:37:40Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1152/physrev.00026.2018"],["dc.identifier.eissn","1522-1210"],["dc.identifier.issn","0031-9333"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/77057"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Sensory Processing at Ribbon Synapses in the Retina and the Cochlea"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]