Büscher, Marita

[["dc.bibliographiccitation.journal","Development"],["dc.contributor.author","Garcia-Perez, Natalia Carolina"],["dc.contributor.author","Bucher, Gregor"],["dc.contributor.author","Buescher, Marita"],["dc.date.accessioned","2021-09-01T06:42:54Z"],["dc.date.available","2021-09-01T06:42:54Z"],["dc.date.issued","2021"],["dc.description.abstract","Gene regulatory mechanisms which specify subtype identity of central complex (CX) neurons are the subject of intense investigation. The CX is a compartment within the brain common to all insect species and functions as a “command center” which directs motor actions. It is made up of several thousand neurons with more than 60 morphologically distinct identities. Accordingly, transcriptional programs must effect the specification of at least as many neuronal subtypes. We demonstrate a role for the transcription factor Shaking hands (Skh) in the specification of embryonic CX neurons in Tribolium. The developmental dynamics of Tc-skh expression are characteristic for terminal selectors of subtype identity. In the embryonic brain, Tc-skh expression is restricted to a subset of neurons, many of which survive to adulthood and contribute to the mature CX. Tc-skh expression is maintained throughout the lifetime in at least some CX neurons. Tc-skh knock-down results in axon outgrowth defects thus preventing the formation of an embryonic CX primordium. The as yet unstudied Drosophila skh shows a similar embryonic expression pattern suggesting that subtype specification of CX neurons may be conserved."],["dc.description.abstract","Gene regulatory mechanisms which specify subtype identity of central complex (CX) neurons are the subject of intense investigation. The CX is a compartment within the brain common to all insect species and functions as a “command center” which directs motor actions. It is made up of several thousand neurons with more than 60 morphologically distinct identities. Accordingly, transcriptional programs must effect the specification of at least as many neuronal subtypes. We demonstrate a role for the transcription factor Shaking hands (Skh) in the specification of embryonic CX neurons in Tribolium. The developmental dynamics of Tc-skh expression are characteristic for terminal selectors of subtype identity. In the embryonic brain, Tc-skh expression is restricted to a subset of neurons, many of which survive to adulthood and contribute to the mature CX. Tc-skh expression is maintained throughout the lifetime in at least some CX neurons. Tc-skh knock-down results in axon outgrowth defects thus preventing the formation of an embryonic CX primordium. The as yet unstudied Drosophila skh shows a similar embryonic expression pattern suggesting that subtype specification of CX neurons may be conserved."],["dc.identifier.doi","10.1242/dev.199368"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/89171"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-455"],["dc.relation.eissn","1477-9129"],["dc.relation.issn","0950-1991"],["dc.title","Shaking hands is a homeodomain transcription factor that controls axon outgrowth of central complex neurons in the insect model Tribolium"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","223"],["dc.bibliographiccitation.journal","Frontiers in Molecular Neuroscience"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Hahn, Nina"],["dc.contributor.author","Knorr, Debbra Y."],["dc.contributor.author","Liebig, Johannes"],["dc.contributor.author","Wüstefeld, Liane"],["dc.contributor.author","Peters, Karsten"],["dc.contributor.author","Ehrenreich, Hannelore"],["dc.contributor.author","Heinrich, Ralf"],["dc.contributor.author","Büscher, Marita"],["dc.contributor.author","Bucher, Gregor"],["dc.date.accessioned","2018-03-08T09:22:14Z"],["dc.date.available","2018-03-08T09:22:14Z"],["dc.date.issued","2017"],["dc.description.abstract","The cytokine erythropoietin (Epo) mediates various cell homeostatic responses to environmental challenges and pathological insults. While stimulation of vertebrate erythrocyte production is mediated by homodimeric “classical” Epo receptors, alternative receptors are involved in neuroprotection. However, their identity remains enigmatic due to complex cytokine ligand and receptor interactions and conflicting experimental results. Besides the classical Epo receptor, the family of type I cytokine receptors also includes the poorly characterized orphan cytokine receptor-like factor 3 (CRLF3) present in vertebrates including human and various insect species. By making use of the more simple genetic makeup of insect model systems, we studied whether CRLF3 is a neuroprotective Epo receptor in animals. We identified a single ortholog of CRLF3 in the beetle Tribolium castaneum, and established protocols for primary neuronal cell cultures from Tribolium brains and efficient in vitro RNA interference. Recombinant human Epo as well as the non-erythropoietic Epo splice variant EV-3 increased the survival of serum-deprived brain neurons, confirming the previously described neuroprotective effect of Epo in insects. Moreover, Epo completely prevented hypoxia-induced apoptotic cell death of primary neuronal cultures. Knockdown of CRLF3 expression by RNA interference with two different double stranded RNA (dsRNA) fragments abolished the neuroprotective effect of Epo, indicating that CRLF3 is a crucial component of the insect Epo-responsive receptor. This suggests that a common urbilaterian ancestor of the orphan human and insect cytokine receptor CRLF3 served as a neuroprotective receptor for an Epo-like cytokine. Our work also suggests that vertebrate CRLF3, like its insect ortholog, might represent a tissue protection-mediating receptor."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2017"],["dc.identifier.doi","10.3389/fnmol.2017.00223"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14838"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/12916"],["dc.language.iso","en"],["dc.notes.intern","GRO-Li-Import"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.publisher","Frontiers Media S.A."],["dc.relation.doi","10.3389/fnmol.2017.00223"],["dc.relation.eissn","1662-5099"],["dc.relation.issn","1662-5099"],["dc.relation.issn","1662-5099"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","The Insect Ortholog of the Human Orphan Cytokine Receptor CRLF3 Is a Neuroprotective Erythropoietin Receptor"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","209"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Development Genes and Evolution"],["dc.bibliographiccitation.lastpage","219"],["dc.bibliographiccitation.volume","226"],["dc.contributor.author","Koniszewski, Nikolaus Dieter Bernhard"],["dc.contributor.author","Kollmann, Martin"],["dc.contributor.author","Bigham, Mahdiyeh"],["dc.contributor.author","Farnworth, M. S."],["dc.contributor.author","He, Bicheng"],["dc.contributor.author","Buescher, Marita"],["dc.contributor.author","Huetteroth, Wolf"],["dc.contributor.author","Binzer, Marlene"],["dc.contributor.author","Schachtner, Joachim"],["dc.contributor.author","Bucher, Gregor"],["dc.date.accessioned","2018-11-07T10:13:29Z"],["dc.date.available","2018-11-07T10:13:29Z"],["dc.date.issued","2016"],["dc.description.abstract","The adult insect brain is composed of neuropils present in most taxa. However, the relative size, shape, and developmental timing differ between species. This diversity of adult insect brain morphology has been extensively described while the genetic mechanisms of brain development are studied predominantly in Drosophila melanogaster. However, it has remained enigmatic what cellular and genetic mechanisms underlie the evolution of neuropil diversity or heterochronic development. In this perspective paper, we propose a novel approach to study these questions. We suggest using genome editing to mark homologous neural cells in the fly D. melanogaster, the beetle Tribolium castaneum, and the Mediterranean field cricket Gryllus bimaculatus to investigate developmental differences leading to brain diversification. One interesting aspect is the heterochrony observed in central complex development. Ancestrally, the central complex is formed during embryogenesis (as in Gryllus) but in Drosophila, it arises during late larval and metamorphic stages. In Tribolium, it forms partially during embryogenesis. Finally, we present tools for brain research in Tribolium including 3D reconstruction and immunohistochemistry data of first instar brains and the generation of transgenic brain imaging lines. Further, we characterize reporter lines labeling the mushroom bodies and reflecting the expression of the neuroblast marker gene Tc-asense, respectively."],["dc.description.sponsorship","DFG Research Center \"Molecular Physiology of the Brain\" (CMPB)"],["dc.identifier.doi","10.1007/s00427-016-0542-7"],["dc.identifier.isi","000377362100008"],["dc.identifier.pmid","27056385"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13296"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/40443"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1432-041X"],["dc.relation.issn","0949-944X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","The insect central complex as model for heterochronic brain development-background, concepts, and tools"],["dc.type","review"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","219"],["dc.bibliographiccitation.lastpage","232"],["dc.bibliographiccitation.seriesnr","2047"],["dc.contributor.author","Buescher, Marita"],["dc.contributor.author","Oberhofer, Georg"],["dc.contributor.author","Garcia-Perez, Natalia Carolina"],["dc.contributor.author","Bucher, Gregor"],["dc.contributor.editor","Sprecher, Simon G."],["dc.date.accessioned","2021-06-02T10:44:27Z"],["dc.date.available","2021-06-02T10:44:27Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1007/978-1-4939-9732-9_12"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/87047"],["dc.notes.intern","DOI-Import GROB-425"],["dc.publisher","Springer New York"],["dc.publisher.place","New York, NY"],["dc.relation.crisseries","Methods in Molecular Biology"],["dc.relation.eisbn","978-1-4939-9732-9"],["dc.relation.isbn","978-1-4939-9731-2"],["dc.relation.ispartof","Methods in Molecular Biology"],["dc.relation.ispartof","Brain Development : Methods and Protocols"],["dc.relation.ispartofseries","Methods in Molecular Biology; 2047"],["dc.title","A Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry for the Study of Embryonic Brain Development in Tribolium castaneum"],["dc.type","book_chapter"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","eLife"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","He, Bicheng"],["dc.contributor.author","Buescher, Marita"],["dc.contributor.author","Farnworth, Max Stephen"],["dc.contributor.author","Strobl, Frederic"],["dc.contributor.author","Stelzer, Ernst HK"],["dc.contributor.author","Koniszewski, Nikolaus DB"],["dc.contributor.author","Muehlen, Dominik"],["dc.contributor.author","Bucher, Gregor"],["dc.date.accessioned","2020-12-10T18:48:08Z"],["dc.date.available","2020-12-10T18:48:08Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.7554/eLife.49065"],["dc.identifier.eissn","2050-084X"],["dc.identifier.pmid","31625505"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16916"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/79031"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","An ancestral apical brain region contributes to the central complex under the control of foxQ2 in the beetle Tribolium"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]