Heimel, Kai

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Genetics"],["dc.bibliographiccitation.volume","220"],["dc.contributor.author","de la Torre, Antonio"],["dc.contributor.author","Jurca, Matteo"],["dc.contributor.author","Hoffmann, Kai"],["dc.contributor.author","Schmitz, Lara"],["dc.contributor.author","Heimel, Kai"],["dc.contributor.author","Kämper, Jörg"],["dc.contributor.author","Pérez-Martín, José"],["dc.contributor.editor","Glass, N L"],["dc.date.accessioned","2022-02-01T10:31:21Z"],["dc.date.available","2022-02-01T10:31:21Z"],["dc.date.issued","2021"],["dc.description.abstract","Abstract Site-specific recombinases have been used in higher eukaryotes, especially in animals, for a broad range of applications, including chromosomal translocations, large deletions, site-specific integration, and tissue-specific as well as conditional knock-outs. The application of site-specific recombination has also been demonstrated in simple eukaryotes like fungi and protozoa. However, its use in fungal research, especially in phytopathogenic fungi, has often been limited to “recycle” the marker genes used in transformation experiments. We show that Cre recombinase can be used for conditional gene deletions in the phytopathogenic fungus Ustilago maydis. Conditional gene knock-outs can be generated via the transcriptional control of the recombinase by U. maydis promoters specifically activated during the biotrophic phase of fungal growth, enabling gene deletions at defined developmental stages inside the plant tissue. Also, we show that a tamoxifen-activated Cre-recombinase allows the tight control necessary for the induced deletion of essential genes by the addition of tamoxifen. These tools will be helpful to address the function of genes under both axenic and in planta conditions for the U. maydis-maize pathosystem and should pave the way for similar approaches in other plant pathosystems."],["dc.description.abstract","Abstract Site-specific recombinases have been used in higher eukaryotes, especially in animals, for a broad range of applications, including chromosomal translocations, large deletions, site-specific integration, and tissue-specific as well as conditional knock-outs. The application of site-specific recombination has also been demonstrated in simple eukaryotes like fungi and protozoa. However, its use in fungal research, especially in phytopathogenic fungi, has often been limited to “recycle” the marker genes used in transformation experiments. We show that Cre recombinase can be used for conditional gene deletions in the phytopathogenic fungus Ustilago maydis. Conditional gene knock-outs can be generated via the transcriptional control of the recombinase by U. maydis promoters specifically activated during the biotrophic phase of fungal growth, enabling gene deletions at defined developmental stages inside the plant tissue. Also, we show that a tamoxifen-activated Cre-recombinase allows the tight control necessary for the induced deletion of essential genes by the addition of tamoxifen. These tools will be helpful to address the function of genes under both axenic and in planta conditions for the U. maydis-maize pathosystem and should pave the way for similar approaches in other plant pathosystems."],["dc.identifier.doi","10.1093/genetics/iyab152"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/98838"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-517"],["dc.relation.eissn","1943-2631"],["dc.title","Robust Cre recombinase activity in the biotrophic smut fungus Ustilago maydis enables efficient conditional null mutants in planta"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","1812"],["dc.bibliographiccitation.journal","F1000Research"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Schmitz, Lara"],["dc.contributor.author","McCotter, Sean"],["dc.contributor.author","Kretschmer, Matthias"],["dc.contributor.author","Kronstad, James W."],["dc.contributor.author","Heimel, Kai"],["dc.date.accessioned","2019-11-13T08:31:54Z"],["dc.date.available","2019-11-13T08:31:54Z"],["dc.date.issued","2018"],["dc.description.abstract","Biotrophic fungal pathogens of plants must sense and adapt to the host environment to complete their life cycles. Recent transcriptome studies of the infection of maize by the biotrophic pathogen Ustilago maydis are providing molecular insights into an ordered program of changes in gene expression and the deployment of effectors as well as key features of nutrient acquisition. In particular, the transcriptome data provide a deeper appreciation of the complexity of the transcription factor network that controls the biotrophic program of invasion, proliferation, and sporulation. Additionally, transcriptome analysis during tumor formation, a key late stage in the life cycle, revealed features of the remodeling of host and pathogen metabolism that may support the formation of tremendous numbers of spores. Transcriptome studies are also appearing for other smut species during interactions with their hosts, thereby providing opportunities for comparative approaches to understand biotrophic adaptation."],["dc.identifier.doi","10.12688/f1000research.16404.1"],["dc.identifier.pmid","30519451"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62613"],["dc.language.iso","en"],["dc.relation.eissn","2046-1402"],["dc.relation.issn","2046-1402"],["dc.title","Transcripts and tumors: regulatory and metabolic programming during biotrophic phytopathogenesis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2388"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","The Plant Cell"],["dc.bibliographiccitation.lastpage","2401"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Scherer, Mario"],["dc.contributor.author","Heimel, Kai"],["dc.contributor.author","Starke, Verena"],["dc.contributor.author","Kämper, Jörg"],["dc.date.accessioned","2022-04-05T15:35:33Z"],["dc.date.available","2022-04-05T15:35:33Z"],["dc.date.issued","2006-09"],["dc.description.abstract","In the phytopathogenic fungus Ustilago maydis, pathogenic development is controlled by a heterodimer of the two homeodomain proteins bE and bW, encoded by the b-mating-type locus. We have identified a b-dependently induced gene, clampless1 (clp1), that is required for the proliferation of dikaryotic filaments in planta. We show that U. maydis hyphae develop structures functionally equivalent to clamp cells that participate in the distribution of nuclei during cell division. In clp1 mutant strains, dikaryotic filaments penetrate the plant cuticle, but development is stalled before the first mitotic division, and the clamp-like structures are not formed. Although clp1 is immediately activated upon b-induction on the transcriptional level, nuclear-localized Clp1 protein is first observed at the stage of plant penetration prior to the first cell division. Induced expression of clp1 strongly interferes with b-dependent gene regulation and blocks b-dependent filament formation and b-dependent cell cycle arrest. We speculate that the Clp1 protein inhibits the activity of the bE/bW heterodimer to facilitate the cell cycle progression during hyphal growth."],["dc.identifier.doi","10.1105/tpc.106.043521"],["dc.identifier.pmid","16920779"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/106404"],["dc.language.iso","en"],["dc.relation.issn","1040-4651"],["dc.title","The Clp1 protein is required for clamp formation and pathogenic development of Ustilago maydis"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4262"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","The Plant Cell"],["dc.bibliographiccitation.lastpage","4277"],["dc.bibliographiccitation.volume","25"],["dc.contributor.author","Heimel, Kai"],["dc.contributor.author","Freitag, Johannes"],["dc.contributor.author","Hampel, Martin"],["dc.contributor.author","Ast, Julia"],["dc.contributor.author","Boelker, Michael"],["dc.contributor.author","Kaemper, Joerg"],["dc.date.accessioned","2018-11-07T09:18:59Z"],["dc.date.available","2018-11-07T09:18:59Z"],["dc.date.issued","2013"],["dc.description.abstract","The unfolded protein response (UPR) is a conserved eukaryotic signaling pathway regulating endoplasmic reticulum (ER) homeostasis during ER stress, which results, for example, from an increased demand for protein secretion. Here, we characterize the homologs of the central UPR regulatory proteins Hac1 (for Homologous to ATF/CREB1) and Inositol Requiring Enzyme1 in the plant pathogenic fungus Ustilago maydis and demonstrate that the UPR is tightly interlinked with the b mating-type-dependent signaling pathway that regulates pathogenic development. Exact timing of UPR is required for virulence, since premature activation interferes with the b-dependent switch from budding to filamentous growth. In addition, we found crosstalk between UPR and the b target Clampless1 (Clp1), which is essential for cell cycle release and proliferation in planta. The unusual C-terminal extension of the U. maydis Hac1 homolog, Cib1 (for Clp1 interacting bZIP1), mediates direct interaction with Clp1. The interaction between Clp1 and Cib1 promotes stabilization of Clp1, resulting in enhanced ER stress tolerance that prevents deleterious UPR hyperactivation. Thus, the interaction between Cib1 and Clp1 constitutes a checkpoint to time developmental progression and increased secretion of effector proteins at the onset of biotrophic development. Crosstalk between UPR and the b mating-type regulated developmental program adapts ER homeostasis to the changing demands during biotrophy."],["dc.identifier.doi","10.1105/tpc.113.115899"],["dc.identifier.isi","000327723100047"],["dc.identifier.pmid","24179126"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28530"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Plant Biologists"],["dc.relation.issn","1532-298X"],["dc.relation.issn","1040-4651"],["dc.title","Crosstalk between the Unfolded Protein Response and Pathways That Regulate Pathogenic Development in Ustilago maydis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e1001035"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","PLoS Pathogens"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Heimel, Kai"],["dc.contributor.author","Scherer, Mario"],["dc.contributor.author","Vranes, Miroslav"],["dc.contributor.author","Wahl, Ramon"],["dc.contributor.author","Pothiratana, Chetsada"],["dc.contributor.author","Schuler, David"],["dc.contributor.author","Vincon, Volker"],["dc.contributor.author","Finkernagel, Florian"],["dc.contributor.author","Flor-Parra, Ignacio"],["dc.contributor.author","Kämper, Jörg"],["dc.date.accessioned","2022-04-05T15:35:54Z"],["dc.date.available","2022-04-05T15:35:54Z"],["dc.date.issued","2010-08-05"],["dc.description.abstract","In the phytopathogenic basidiomycete Ustilago maydis, sexual and pathogenic development are tightly connected and controlled by the heterodimeric bE/bW transcription factor complex encoded by the b-mating type locus. The formation of the active bE/bW heterodimer leads to the formation of filaments, induces a G2 cell cycle arrest, and triggers pathogenicity. Here, we identify a set of 345 bE/bW responsive genes which show altered expression during these developmental changes; several of these genes are associated with cell cycle coordination, morphogenesis and pathogenicity. 90% of the genes that show altered expression upon bE/bW-activation require the zinc finger transcription factor Rbf1, one of the few factors directly regulated by the bE/bW heterodimer. Rbf1 is a novel master regulator in a multilayered network of transcription factors that facilitates the complex regulatory traits of sexual and pathogenic development."],["dc.identifier.doi","10.1371/journal.ppat.1001035"],["dc.identifier.pmid","20700446"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/106406"],["dc.language.iso","en"],["dc.relation.eissn","1553-7374"],["dc.title","The transcription factor Rbf1 is the master regulator for b-mating type controlled pathogenic development in Ustilago maydis"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e1007734"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","PLOS Pathogens"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Pinter, Niko"],["dc.contributor.author","Hach, Christina Andrea"],["dc.contributor.author","Hampel, Martin"],["dc.contributor.author","Rekhter, Dmitrij"],["dc.contributor.author","Zienkiewicz, Krzysztof"],["dc.contributor.author","Feußner, Ivo"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Finkernagel, Florian"],["dc.contributor.author","Heimel, Kai"],["dc.date.accessioned","2019-07-09T11:51:17Z"],["dc.date.available","2019-07-09T11:51:17Z"],["dc.date.issued","2019"],["dc.description.abstract","The corn smut fungus Ustilago maydis requires the unfolded protein response (UPR) to maintain homeostasis of the endoplasmic reticulum (ER) during the biotrophic interaction with its host plant Zea mays (maize). Crosstalk between the UPR and pathways controlling pathogenic development is mediated by protein-protein interactions between the UPR regulator Cib1 and the developmental regulator Clp1. Cib1/Clp1 complex formation results in mutual modification of the connected regulatory networks thereby aligning fungal proliferation in planta, efficient effector secretion with increased ER stress tolerance and long-term UPR activation in planta. Here we address UPR-dependent gene expression and its modulation by Clp1 using combinatorial RNAseq/ChIPseq analyses. We show that increased ER stress resistance is connected to Clp1-dependent alterations of Cib1 phosphorylation, protein stability and UPR gene expression. Importantly, we identify by deletion screening of UPR core genes the signal peptide peptidase Spp1 as a novel key factor that is required for establishing a compatible biotrophic interaction between U. maydis and its host plant maize. Spp1 is dispensable for ER stress resistance and vegetative growth but requires catalytic activity to interfere with the plant defense, revealing a novel virulence specific function for signal peptide peptidases in a biotrophic fungal/plant interaction."],["dc.identifier.doi","10.1371/journal.ppat.1007734"],["dc.identifier.pmid","30998787"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16094"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59916"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","572"],["dc.title","Signal peptide peptidase activity connects the unfolded protein response to plant defense suppression by Ustilago maydis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0153861"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Hampel, Martin"],["dc.contributor.author","Jakobi, Mareike"],["dc.contributor.author","Schmitz, Lara"],["dc.contributor.author","Meyer, Ute"],["dc.contributor.author","Finkernagel, Florian"],["dc.contributor.author","Doehlemann, Gunther"],["dc.contributor.author","Heimel, Kai"],["dc.date.accessioned","2018-11-07T10:15:28Z"],["dc.date.available","2018-11-07T10:15:28Z"],["dc.date.issued","2016"],["dc.description.abstract","The unfolded protein response (UPR), a conserved eukaryotic signaling pathway to ensure protein homeostasis in the endoplasmic reticulum (ER), coordinates biotrophic development in the corn smut fungus Ustilago maydis. Exact timing of UPR activation is required for virulence and presumably connected to the elevated expression of secreted effector proteins during infection of the host plant Zea mays. In the baker's yeast Saccharomyces cerevisiae, expression of UPR target genes is induced upon binding of the central regulator Hac1 to unfolded protein response elements (UPREs) in their promoters. While a role of the UPR in effector secretion has been described previously, we investigated a potential UPR-dependent regulation of genes encoding secreted effector proteins. In silico prediction of UPREs in promoter regions identified the previously characterized effector genes pit2 and tin1-1, as bona fide UPR target genes. Furthermore, direct binding of the Hac1-homolog Cib1 to the UPRE containing promoter fragments of both genes was confirmed by quantitative chromatin immunoprecipitation (qChIP) analysis. Targeted deletion of the UPRE abolished Cib1-dependent expression of pit2 and significantly affected virulence. Furthermore, ER stress strongly increased Pit2 expression and secretion. This study expands the role of the UPR as a signal hub in fungal virulence and illustrates, how biotrophic fungi can coordinate cellular physiology, development and regulation of secreted virulence factors."],["dc.description.sponsorship","Open-Access Publikationsfonds 2016"],["dc.identifier.doi","10.1371/journal.pone.0153861"],["dc.identifier.isi","000374541200052"],["dc.identifier.pmid","27093436"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13232"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/40816"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Unfolded Protein Response (UPR) Regulator Cib1 Controls Expression of Genes Encoding Secreted Virulence Factors in Ustilago maydis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","mBio"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Schmitz, Lara"],["dc.contributor.author","Schwier, Melina Ayaka"],["dc.contributor.author","Heimel, Kai"],["dc.contributor.editor","Lin, Xiaorong"],["dc.date.accessioned","2020-12-10T18:37:05Z"],["dc.date.available","2020-12-10T18:37:05Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1128/mBio.02756-19"],["dc.identifier.eissn","2150-7511"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17178"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/76832"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","The Unfolded Protein Response Regulates Pathogenic Development of Ustilago maydis by Rok1-Dependent Inhibition of Mating-Type Signaling"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1118"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Molecular Plant-Microbe Interactions"],["dc.bibliographiccitation.lastpage","1129"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Zahiri, Alexander"],["dc.contributor.author","Heimel, Kai"],["dc.contributor.author","Wahl, Ramon"],["dc.contributor.author","Rath, Magnus"],["dc.contributor.author","Kämper, Jörg"],["dc.date.accessioned","2019-11-13T08:34:49Z"],["dc.date.available","2019-11-13T08:34:49Z"],["dc.date.issued","2010"],["dc.description.abstract","Ustilago maydis is a plant-pathogenic fungus that establishes a biotrophic relationship with its host plant, Zea mays. The pathogenic stage of U. maydis is initiated by the fusion of two haploid cells, resulting in the formation of a dikaryotic hypha that invades the plant cell. The switch from saprophytic, yeast-like cells to the biotrophic hyphae requires the complex regulation of a multitude of biological processes to constitute the compatible host-fungus interaction. Transcriptional regulators involved in the establishment of the infectious dikaryon and penetration of the host tissue have been identified; however, regulators required during the post-penetration stages remained to be elucidated. In this study, we report the identification of a U. maydis forkhead transcription factor, Fox1, which is exclusively expressed during biotrophic development. Deletion of fox1 results in reduced virulence and impaired tumor development. The Deltafox1 hyphae induce the accumulation of H(2)O(2) in and around infected cells and a maize defense response phenotypically represented by the encasement of proliferating hyphae in a cellulose-containing matrix. The phenotype can be attributed to the fox1-dependent deregulation of several effector genes that are linked to pathogenic development and host defense suppression."],["dc.identifier.doi","10.1094/MPMI-23-9-1118"],["dc.identifier.pmid","20687802"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62614"],["dc.language.iso","en"],["dc.relation.issn","0894-0282"],["dc.title","The Ustilago maydis forkhead transcription factor Fox1 is involved in the regulation of genes required for the attenuation of plant defenses during pathogenic development"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","305"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Journal of Fungi"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Starke, Jessica"],["dc.contributor.author","Harting, Rebekka"],["dc.contributor.author","Maurus, Isabel"],["dc.contributor.author","Leonard, Miriam"],["dc.contributor.author","Bremenkamp, Rica"],["dc.contributor.author","Heimel, Kai"],["dc.contributor.author","Kronstad, James W."],["dc.contributor.author","Braus, Gerhard H."],["dc.date.accessioned","2021-06-01T09:42:38Z"],["dc.date.available","2021-06-01T09:42:38Z"],["dc.date.issued","2021"],["dc.description.abstract","Differentiation, growth, and virulence of the vascular plant pathogen Verticillium dahliae depend on a network of interconnected cellular signaling cascades. The transcription factor Hac1 of the endoplasmic reticulum-associated unfolded protein response (UPR) is required for initial root colonization, fungal growth, and vascular propagation by conidiation. Hac1 is essential for the formation of microsclerotia as long-time survival resting structures in the field. Single endoplasmic reticulum-associated enzymes for linoleic acid production as precursors for oxylipin signal molecules support fungal growth but not pathogenicity. Microsclerotia development, growth, and virulence further require the pheromone response mitogen-activated protein kinase (MAPK) pathway, but without the Ham5 scaffold function. The MAPK phosphatase Rok1 limits resting structure development of V.dahliae, but promotes growth, conidiation, and virulence. The interplay between UPR and MAPK signaling cascades includes several potential targets for fungal growth control for supporting disease management of the vascular pathogen V.dahliae."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.3390/jof7040305"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85307"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","2309-608X"],["dc.relation.orgunit","Abteilung Molekulare Mikrobiologie & Genetik"],["dc.rights","CC BY 4.0"],["dc.title","Unfolded Protein Response and Scaffold Independent Pheromone MAP Kinase Signaling Control Verticillium dahliae Growth, Development, and Plant Pathogenesis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]