Savić, Filip

[["dc.bibliographiccitation.journal","European Biophysics Journal"],["dc.contributor.author","Witt, Hannes"],["dc.contributor.author","Savić, Filip"],["dc.contributor.author","Verbeek, Sarah"],["dc.contributor.author","Dietz, Jörn"],["dc.contributor.author","Tarantola, Gesa"],["dc.contributor.author","Oelkers, Marieelen"],["dc.contributor.author","Geil, Burkhard"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2021-04-14T08:29:19Z"],["dc.date.available","2021-04-14T08:29:19Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1007/s00249-020-01490-5"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82863"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1432-1017"],["dc.relation.issn","0175-7571"],["dc.title","Membrane fusion studied by colloidal probes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2216"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","2228"],["dc.bibliographiccitation.volume","110"],["dc.contributor.author","Savic, Filip"],["dc.contributor.author","Kliesch, Torben-Tobias"],["dc.contributor.author","Verbeek, Sarah"],["dc.contributor.author","Bao, Chunxiao"],["dc.contributor.author","Thiart, Jan"],["dc.contributor.author","Kros, Alexander"],["dc.contributor.author","Geil, Burkhard"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T10:14:08Z"],["dc.date.available","2018-11-07T10:14:08Z"],["dc.date.issued","2016"],["dc.description.abstract","The fusion of lipid membranes is a key process in biology. It enables cells and organelles to exchange molecules with their surroundings, which otherwise could not cross the membrane barrier. To study such complex processes we use simplified artificial model systems, i.e., an optical fusion assay based on membrane-coated glass spheres. We present a technique to analyze membrane-membrane interactions in a large ensemble of particles. Detailed information on the geometry of the fusion stalk of fully fused membranes is obtained by studying the diffusional lipid dynamics with fluorescence recovery after photo-bleaching experiments. A small contact zone is a strong obstruction for the particle exchange across the fusion spot. With the aid of computer simulations, fluorescence-recovery-after-photobleaching recovery times of both fused and single-membrane-coated beads allow us to estimate the size of the contact zones between two membrane-coated beads. Minimizing delamination and bending energy leads to minimal angles close to those geometrically allowed."],["dc.description.sponsorship","SFB [803 (B08)]"],["dc.identifier.doi","10.1016/j.bpj.2016.04.026"],["dc.identifier.isi","000376436700012"],["dc.identifier.pmid","27224487"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/40569"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.relation.issn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.title","Geometry of the Contact Zone between Fused Membrane-Coated Beads Mimicking Cell-Cell Fusion"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","E6064"],["dc.bibliographiccitation.issue","30"],["dc.bibliographiccitation.journal","Proceedings of the National Academy of Sciences of the United States of America"],["dc.bibliographiccitation.lastpage","E6071"],["dc.bibliographiccitation.volume","114"],["dc.contributor.author","Schütte, Ole M."],["dc.contributor.author","Mey, Ingo"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Savić, Filip"],["dc.contributor.author","Geil, Burkhard"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2018-01-17T13:02:19Z"],["dc.date.available","2018-01-17T13:02:19Z"],["dc.date.issued","2017"],["dc.description.abstract","In the plasma membrane of eukaryotic cells, proteins and lipids are organized in clusters, the latter ones often called lipid domains or \"lipid rafts.\" Recent findings highlight the dynamic nature of such domains and the key role of membrane geometry and spatial boundaries. In this study, we used porous substrates with different pore radii to address precisely the extent of the geometric constraint, permitting us to modulate and investigate the size and mobility of lipid domains in phase-separated continuous pore-spanning membranes (PSMs). Fluorescence video microscopy revealed two types of liquid-ordered (lo) domains in the freestanding parts of the PSMs: (i) immobile domains that were attached to the pore rims and (ii) mobile, round-shaped lo domains within the center of the PSMs. Analysis of the diffusion of the mobile lo domains by video microscopy and particle tracking showed that the domains' mobility is slowed down by orders of magnitude compared with the unrestricted case. We attribute the reduced mobility to the geometric confinement of the PSM, because the drag force is increased substantially due to hydrodynamic effects generated by the presence of these boundaries. Our system can serve as an experimental test bed for diffusion of 2D objects in confined geometry. The impact of hydrodynamics on the mobility of enclosed lipid domains can have great implications for the formation and lateral transport of signaling platforms."],["dc.identifier.doi","10.1073/pnas.1704199114"],["dc.identifier.pmid","28696315"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11700"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1091-6490"],["dc.title","Size and mobility of lipid domains tuned by geometrical constraints"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1582"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","1592"],["dc.bibliographiccitation.volume","110"],["dc.contributor.author","Witt, Hannes"],["dc.contributor.author","Savic, Filip"],["dc.contributor.author","Oelkers, Marieelen"],["dc.contributor.author","Awan, Shahid I."],["dc.contributor.author","Werz, Daniel B."],["dc.contributor.author","Geil, Burkhard"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T10:15:33Z"],["dc.date.available","2018-11-07T10:15:33Z"],["dc.date.issued","2016"],["dc.description.abstract","Weak noncovalent intermolecular interactions play a pivotal role in many biological processes such as cell adhesion or immunology, where the overall binding strength is controlled through bond association and dissociation dynamics as well as the cooperative action of many parallel bonds. Among the various molecules participating in weak bonds, carbohydrate-carbohydrate interactions are probably the most ancient ones allowing individual cells to reversibly enter the multicellular state and to tell apart self and nonself cells. Here, we scrutinized the kinetics and thermodynamics of small homomeric Lewis X-Lewis X ensembles formed in the contact zone of a membrane-coated colloidal probe and a solid supported membrane ensuring minimal nonspecific background interactions. We used an atomic force microscope to measure force distance curves at Piconewton resolution, which allowed us to measure the force due to unbinding of the colloidal probe and the planar membrane as a function of contact time. Applying a contact model, we could estimate the free binding energy of the formed adhesion cluster as a function of dwell time and thereby determine the precise size of the contact zone, the number of participating bonds, and the intrinsic rates of association and dissociation in the presence of calcium ions. The unbinding energy per bond was found to be on the order of 1 k(B)T. Approximately 30 bonds were opened simultaneously at an off-rate of k(off) = 7 +/- 0.2 s(-1)."],["dc.identifier.doi","10.1016/j.bpj.2016.03.006"],["dc.identifier.isi","000374209700012"],["dc.identifier.pmid","27074683"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/40833"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.relation.issn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.title","Size, Kinetics, and Free Energy of Clusters Formed by Ultraweak Carbohydrate-Carbohydrate Bonds"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","European Biophysics Journal"],["dc.bibliographiccitation.volume","44"],["dc.contributor.author","Witt, Hannes"],["dc.contributor.author","Oelkers, Marieelen"],["dc.contributor.author","Savic, Filip"],["dc.contributor.author","Awan, S."],["dc.contributor.author","Werz, Daniel B."],["dc.contributor.author","Geil, Burkhard"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T09:55:36Z"],["dc.date.available","2018-11-07T09:55:36Z"],["dc.date.issued","2015"],["dc.format.extent","S244"],["dc.identifier.isi","000380001400772"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36784"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","New york"],["dc.relation.eventlocation","Dresden, GERMANY"],["dc.relation.issn","1432-1017"],["dc.relation.issn","0175-7571"],["dc.title","Binding enthalpy driven accumulation of Lewis X in the membrane-membrane contact zone"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","European Biophysics Journal"],["dc.bibliographiccitation.volume","44"],["dc.contributor.author","Bao, Chunxiao"],["dc.contributor.author","Savic, Filip"],["dc.contributor.author","Kliesch, T.-T."],["dc.contributor.author","Verbeek, Sarah"],["dc.contributor.author","Paehler, Gesa"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T09:55:30Z"],["dc.date.available","2018-11-07T09:55:30Z"],["dc.date.issued","2015"],["dc.format.extent","S189"],["dc.identifier.isi","000380001400561"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36756"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","New york"],["dc.relation.eventlocation","Dresden, GERMANY"],["dc.relation.issn","1432-1017"],["dc.relation.issn","0175-7571"],["dc.title","Optical fusion assay based on membrane-coated spheres in a 2D assembly"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]