Testa, Ilaria

[["dc.bibliographiccitation.artnumber","7368"],["dc.bibliographiccitation.firstpage","204"],["dc.bibliographiccitation.journal","Nature"],["dc.bibliographiccitation.lastpage","208"],["dc.bibliographiccitation.volume","478"],["dc.contributor.author","Grotjohann, Tim"],["dc.contributor.author","Testa, Ilaria"],["dc.contributor.author","Leutenegger, Marcel"],["dc.contributor.author","Bock, Hannes"],["dc.contributor.author","Urban, Nicolai T."],["dc.contributor.author","Lavoie-Cardinal, Flavie"],["dc.contributor.author","Willig, Katrin I."],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2017-09-07T11:43:21Z"],["dc.date.available","2017-09-07T11:43:21Z"],["dc.date.issued","2011"],["dc.description.abstract","Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for decades, but the recent breaking of the diffraction resolution barrier by sequentially switching the fluorescence capability of adjacent features on and off is making nanoscale imaging routine. Reported fluorescence nanoscopy variants switch these features either with intense beams at defined positions or randomly, molecule by molecule. Here we demonstrate an optical nanoscopy that records raw data images from living cells and tissues with low levels of light. This advance has been facilitated by the generation of reversibly switchable enhanced green fluorescent protein (rsEGFP), a fluorescent protein that can be reversibly photoswitched more than a thousand times. Distributions of functional rsEGFP-fusion proteins in living bacteria and mammalian cells are imaged at <40-nanometre resolution. Dendritic spines in living brain slices are super-resolved with about a million times lower light intensities than before. The reversible switching also enables all-optical writing of features with subdiffraction size and spacings, which can be used for data storage."],["dc.identifier.doi","10.1038/nature10497"],["dc.identifier.gro","3142644"],["dc.identifier.isi","000295782800041"],["dc.identifier.pmid","21909116"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0028-0836"],["dc.title","Diffraction-unlimited all-optical imaging and writing with a photochromic GFP"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","European biophysics journal : with biophysics letters"],["dc.bibliographiccitation.volume","40"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Andresen, Martin"],["dc.contributor.author","Brakemann, Tanja"],["dc.contributor.author","Stiel, Andre C."],["dc.contributor.author","Testa, Ilaria"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Jakobs, Stefan"],["dc.date.accessioned","2017-09-07T11:45:52Z"],["dc.date.available","2017-09-07T11:45:52Z"],["dc.date.issued","2011"],["dc.identifier.gro","3145539"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/3248"],["dc.notes.intern","lifescience"],["dc.notes.status","public"],["dc.notes.submitter","oschaef1"],["dc.publisher","Springer"],["dc.relation.conference","8th EBSA European Biophysics Congress"],["dc.relation.eissn","1432-1017"],["dc.relation.eventend","2011-08-27"],["dc.relation.eventlocation","Budapest, Hungary"],["dc.relation.eventstart","2011-08-23"],["dc.relation.issn","0175-7571"],["dc.title","A new class of reversibly switchable fluorescent proteins"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","992"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Neuron"],["dc.bibliographiccitation.lastpage","1000"],["dc.bibliographiccitation.volume","75"],["dc.contributor.author","Testa, Ilaria"],["dc.contributor.author","Urban, Nicolai T."],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Willig, Katrin I."],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:48:25Z"],["dc.date.available","2017-09-07T11:48:25Z"],["dc.date.issued","2012"],["dc.description.abstract","Lens-based fluorescence microscopy, which has long been limited in resolution to about 200 nanometers by diffraction, is rapidly evolving into a nanoscale imaging technique. Here, we show that the superresolution fluorescence microscopy called RESOLFT enables comparatively fast and continuous imaging of sensitive, nanosized features in living brain tissue. Using low-intensity illumination to switch photochromic fluorescent proteins reversibly between a fluorescent and a nonfluorescent state, we increased the resolution more than 3-fold over that of confocal microscopy in all dimensions. Dendritic spines located 10-50 mu m deep inside living organotypic hippocampal brain slices were recorded for hours without signs of degradation. Using a fast-switching protein increased the imaging speed 50-fold over reported RESOLFT schemes, which in turn enabled the recording of spontaneous and stimulated changes of dendritic actin filaments and spine morphology occurring on time scales from seconds to hours."],["dc.identifier.doi","10.1016/j.neuron.2012.07.028"],["dc.identifier.gro","3142464"],["dc.identifier.isi","000309198900011"],["dc.identifier.pmid","22998868"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/8574"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Cell Press"],["dc.relation.issn","0896-6273"],["dc.title","Nanoscopy of Living Brain Slices with Low Light Levels"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Protein science : PS / Supplement"],["dc.bibliographiccitation.volume","21"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Grotjohann, Tim"],["dc.contributor.author","Testa, Ilaria"],["dc.contributor.author","Leuschner, Ivo"],["dc.contributor.author","Eggeling, Christian"],["dc.date.accessioned","2017-09-07T11:45:51Z"],["dc.date.available","2017-09-07T11:45:51Z"],["dc.date.issued","2012"],["dc.format.extent","163"],["dc.identifier.gro","3145547"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/3256"],["dc.notes.intern","lifescience"],["dc.notes.status","public"],["dc.notes.submitter","oschaef1"],["dc.publisher","Wiley-Blackwell"],["dc.relation.conference","26th Annual Symposium of the Protein-Society"],["dc.relation.eissn","1469-896X"],["dc.relation.eventend","2012-08-08"],["dc.relation.eventlocation","San Diego; CA"],["dc.relation.eventstart","2012-08-05"],["dc.relation.issn","0961-8368"],["dc.title","Reversibly switchable rnhanced green fluorescent protein (rsEGFP): A novel photochromic fluorescent protein enables RESOLFT-type microscopy of living cells at low light intensities"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","534a"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","104"],["dc.contributor.author","Testa, Ilaria"],["dc.contributor.author","Urban, Nicolai"],["dc.contributor.author","Willig, Katrin"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2022-03-01T11:44:56Z"],["dc.date.available","2022-03-01T11:44:56Z"],["dc.date.issued","2013"],["dc.identifier.doi","10.1016/j.bpj.2012.11.2958"],["dc.identifier.pii","S000634951204204X"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103168"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.issn","0006-3495"],["dc.title","Resolft Nanoscopy in Life Sciences: Unraveling Fine Details with Low Light Levels"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Protein Science / Supplement"],["dc.bibliographiccitation.volume","21"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Brakemann, Tanja"],["dc.contributor.author","Stiel, Andre C."],["dc.contributor.author","Weber, Gert"],["dc.contributor.author","Andresen, Martin"],["dc.contributor.author","Testa, Ilaria"],["dc.contributor.author","Urlaub, Henning"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Wahl, Markus C."],["dc.contributor.author","Jakobs, Stefan"],["dc.date.accessioned","2017-09-07T11:45:52Z"],["dc.date.available","2017-09-07T11:45:52Z"],["dc.date.issued","2012"],["dc.format.extent","164"],["dc.identifier.gro","3145551"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/3261"],["dc.notes.intern","lifescience"],["dc.notes.status","public"],["dc.notes.submitter","oschaef1"],["dc.relation.eissn","1469-896X"],["dc.relation.issn","0961-8368"],["dc.title","Dreiklang - the one, two, three in photoswitching"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1703333"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Small"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Nevskyi, Oleksii"],["dc.contributor.author","Sysoiev, Dmytro"],["dc.contributor.author","Dreier, Jes"],["dc.contributor.author","Stein, Simon Christoph"],["dc.contributor.author","Oppermann, Alex"],["dc.contributor.author","Lemken, Florian"],["dc.contributor.author","Janke, Tobias"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Testa, Ilaria"],["dc.contributor.author","Huhn, Thomas"],["dc.contributor.author","Wöll, Dominik"],["dc.date.accessioned","2022-03-01T11:46:48Z"],["dc.date.available","2022-03-01T11:46:48Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1002/smll.201703333"],["dc.identifier.issn","1613-6810"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103804"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.issn","1613-6810"],["dc.title","Fluorescent Diarylethene Photoswitches-A Universal Tool for Super-Resolution Microscopy in Nanostructured Materials"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","9592"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Ratz, Michael"],["dc.contributor.author","Testa, Ilaria"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Jakobs, Stefan"],["dc.date.accessioned","2017-09-07T11:44:27Z"],["dc.date.available","2017-09-07T11:44:27Z"],["dc.date.issued","2015"],["dc.description.abstract","Overexpression is a notorious concern in conventional and especially in super-resolution fluorescence light microscopy studies because it may cause numerous artifacts including ectopic sub-cellular localizations, erroneous formation of protein complexes, and others. Nonetheless, current live cell super-resolution microscopy studies generally rely on the overexpression of a host protein fused to a fluorescent protein. Here, we establish CRISPR/Cas9-mediated generation of heterozygous and homozygous human knockin cell lines expressing fluorescently tagged proteins from their respective native genomic loci at close to endogenous levels. We tagged three different proteins, exhibiting various localizations and expression levels, with the reversibly switchable fluorescent protein rsEGFP2. We demonstrate the benefit of endogenous expression levels compared to overexpression and show that typical overexpression-induced artefacts were avoided in genome-edited cells. Fluorescence activated cell sorting analysis revealed a narrow distribution of fusion protein expression levels in genome-edited cells, compared to a pronounced variability in transiently transfected cells. Using low light intensity RESOLFT (reversible saturable optical fluorescence transitions) nanoscopy we show sub-diffraction resolution imaging of living human knockin cells. Our strategy to generate human cell lines expressing fluorescent fusion proteins at endogenous levels for RESOLFT nanoscopy can be extended to other fluorescent tags and super-resolution approaches."],["dc.identifier.doi","10.1038/srep09592"],["dc.identifier.gro","3141924"],["dc.identifier.isi","000353276500001"],["dc.identifier.pmid","25892259"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13621"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2589"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","2045-2322"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","CRISPR/Cas9-mediated endogenous protein tagging for RESOLFT super-resolution microscopy of living human cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","655"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","ChemPhysChem"],["dc.bibliographiccitation.lastpage","663"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Lavoie-Cardinal, Flavie"],["dc.contributor.author","Jensen, Nickels A."],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Stiel, Andre C."],["dc.contributor.author","Chmyrov, Andriy"],["dc.contributor.author","Bierwagen, Jakob"],["dc.contributor.author","Testa, Ilaria"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2017-09-07T11:46:25Z"],["dc.date.available","2017-09-07T11:46:25Z"],["dc.date.issued","2014"],["dc.description.abstract","Up to now, all demonstrations of reversible saturable optical fluorescence transitions (RESOLFT) superresolution microscopy of living cells have relied on the use of reversibly switchable fluorescent proteins (RSFP) emitting in the green spectral range. Here we show RESOLFT imaging with rsCherryRev1.4, a new red-emitting RSFP enabling a spatial resolution up to four times higher than the diffraction barrier. By co-expressing green and red RSFPs in living cells we demonstrate two-color RESOLFT imaging both for single (donut) beam scanning and for parallelized versions of RESOLFT nanoscopy where an array of >23000 donut-like minima are scanned simultaneously."],["dc.identifier.doi","10.1002/cphc.201301016"],["dc.identifier.gro","3142168"],["dc.identifier.isi","000332747500013"],["dc.identifier.pmid","24449030"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12826"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/5288"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Deutsche Forschungsgemeinschaft"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1439-7641"],["dc.relation.issn","1439-4235"],["dc.rights","CC BY-NC-ND 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-nd/3.0"],["dc.title","Two-Color RESOLFT Nanoscopy with Green and Red Fluorescent Photochromic Proteins"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","8488"],["dc.bibliographiccitation.issue","45"],["dc.bibliographiccitation.journal","The Journal of Neuroscience"],["dc.bibliographiccitation.lastpage","8497"],["dc.bibliographiccitation.volume","42"],["dc.contributor.author","Fuhrmann, Martin"],["dc.contributor.author","Gockel, Nala"],["dc.contributor.author","Arizono, Misa"],["dc.contributor.author","Dembitskaya, Yulia"],["dc.contributor.author","Nägerl, U. Valentin"],["dc.contributor.author","Pennacchietti, Francesca"],["dc.contributor.author","Damenti, Martina"],["dc.contributor.author","Testa, Ilaria"],["dc.contributor.author","Willig, Katrin I."],["dc.date.accessioned","2022-12-01T08:31:28Z"],["dc.date.available","2022-12-01T08:31:28Z"],["dc.date.issued","2022"],["dc.identifier.doi","10.1523/JNEUROSCI.1125-22.2022"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/118176"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-621"],["dc.relation.eissn","1529-2401"],["dc.relation.issn","0270-6474"],["dc.title","Super-Resolution Microscopy Opens New Doors to Life at the Nanoscale"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]