Kössler, Philip

[["dc.bibliographiccitation.firstpage","3000"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Toxins"],["dc.bibliographiccitation.lastpage","3011"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Al-Hadithi, Nabil"],["dc.contributor.author","Koessler, Philip"],["dc.contributor.author","Karlovsky, Petr"],["dc.date.accessioned","2018-11-07T09:53:45Z"],["dc.date.available","2018-11-07T09:53:45Z"],["dc.date.issued","2015"],["dc.description.abstract","Solid bar microextraction (SBME), followed by liquid chromatography with fluorescence detection (HPLC-FLD), for the quantification of ochratoxin A in wheat and maize was developed. Ground wheat and maize grains were extracted with acetonitrile-water-acetic acid (79:20:1, v/v/v), followed by defatting with cyclohexane, and subjected to SBME-LC-FLD analysis. SBME devices were constructed by packing 2 mg sorbent (C18) into porous polypropylene micro-tubes (2.5 cm length, 600 mu m i.d., and 0.2 mu m pore size). SBME devices were conditioned with methanol and placed into 5 mL stirred sample solutions for 70 min. After extraction, OTA was desorbed into 200 mu L of methanol for 15 min, the solution was removed in vacuum, the residue was dissolved in 50 mu L of methanol-water (1:1, v/v) and ochratoxin A content was determined by HPLC-FLD. Under optimized extraction conditions, the limit of detection of 0.9 mu g center dot kg(-1) and 2.5 mu g center dot kg(-1) and the precision of 3.4% and 5.0% over a concentration range of 1 to 100 mu g center dot kg(-1) in wheat and maize flour, respectively, were obtained."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2015"],["dc.identifier.doi","10.3390/toxins7083000"],["dc.identifier.isi","000360202700018"],["dc.identifier.pmid","26251923"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12389"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36390"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Mdpi Ag"],["dc.relation.issn","2072-6651"],["dc.rights.access","openAccess"],["dc.title","Determination of Ochratoxin A in Wheat and Maize by Solid Bar Microextraction with Liquid Chromatography and Fluorescence Detection"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","261"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Food Technology and Biotechnology"],["dc.bibliographiccitation.lastpage","268"],["dc.bibliographiccitation.volume","53"],["dc.contributor.author","Trümper, Christina"],["dc.contributor.author","Paffenholz, Katrin"],["dc.contributor.author","Smit, Inga"],["dc.contributor.author","Kössler, Philip"],["dc.contributor.author","Karlovsky, Petr"],["dc.contributor.author","Braun, Hans Peter"],["dc.contributor.author","Pawelzik, Elke"],["dc.date.accessioned","2018-09-10T08:34:51Z"],["dc.date.available","2018-09-10T08:34:51Z"],["dc.date.issued","2015"],["dc.description.abstract","This study was conducted to improve the knowledge of molecular processes involved in the interaction between Fusarium graminearum and emmer in the course of grain ripening. Emmer plants were artificially inoculated with a F. graminearum spore suspension at anthesis. In the course of grain ripening from milk ripe to plant death stage, grains at four phenological growth stages were collected for analysis. The infection degree was evaluated based on the F. graminearum DNA content in emmer grain infolding tissues (glumes and rachis). For proteome analysis the albumin and globulin fractions of emmer grains, consisting of proteins with various functions related to the development and stress response, were analysed regarding the changes due to Fusarium infection by two-dimensional gel electrophoresis. Altogether, forty-three proteins affected by infection were identified by mass spectrometry. Enzymes detoxifying reactive oxygen species were regulated at all developmental stages. In the early stage of grain development, the abundance of proteins related to stress response, such as 2-Cys peroxiredoxin, a chitinase, a xylanase inhibitor and a spermidine synthase was increased. During later stage of grain development, the abundance of stress-related proteins, such as chitinases, heat shock proteins and an α-amylase inhibitor-like protein, decreased. During all ripening stages, but especially during medium milk stage (BBCH 75) and soft dough stage (BBCH 85), the abundance of proteins related to carbon metabolism, starch and protein biosynthesis as well as photosynthesis increased due to F. graminearum infection. At the plant death stage (BBCH 97) the abundance of only two proteins related to metabolism decreased."],["dc.identifier.doi","10.17113/ftb.53.03.15.3838"],["dc.identifier.pmid","27904357"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/15682"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.title","Identification of Differently Regulated Proteins after
Fusarium graminearum Infection of Emmer (Triticum dicoccum) at Several Grain Ripening Stages"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","75"],["dc.bibliographiccitation.journal","Applied Soil Ecology"],["dc.bibliographiccitation.lastpage","82"],["dc.bibliographiccitation.volume","124"],["dc.contributor.author","Lukas, Stefan"],["dc.contributor.author","Abbas, Sayed Jaffar"],["dc.contributor.author","Kössler, Philip"],["dc.contributor.author","Karlovsky, Petr"],["dc.contributor.author","Potthoff, Martin"],["dc.contributor.author","Joergensen, Rainer Georg"],["dc.date.accessioned","2020-12-10T14:22:27Z"],["dc.date.available","2020-12-10T14:22:27Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1016/j.apsoil.2017.10.018"],["dc.identifier.issn","0929-1393"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71618"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Fungal plant pathogens on inoculated maize leaves in a simulated soil warming experiment"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","86"],["dc.bibliographiccitation.journal","Journal of Proteomics"],["dc.bibliographiccitation.lastpage","92"],["dc.bibliographiccitation.volume","133"],["dc.contributor.author","Trümper, Christina"],["dc.contributor.author","Paffenholz, Katrin"],["dc.contributor.author","Smit, Inga"],["dc.contributor.author","Kössler, Philip"],["dc.contributor.author","Karlovsky, Petr"],["dc.contributor.author","Braun, Hans-Peter"],["dc.contributor.author","Pawelzik, Elke"],["dc.date.accessioned","2018-09-10T08:36:13Z"],["dc.date.available","2018-09-10T08:36:13Z"],["dc.date.issued","2016"],["dc.description.abstract","We analyzed the effect of Fusarium graminearum infection on field-grown naked barley (Hordeum vulgare nudum). The ears were inoculated with F. graminearum spores during anthesis. In the course of ripening, grains in five phenological growth stages of naked barley from milk ripe to plant death were sampled. The albumin and globulin proteins of inoculated grains and untreated (control) grains were separated by two-dimensional gel electrophoresis. Forty-five spots composing of proteins that were changed in abundance due to F. graminearum infection were subsequently identified by mass spectrometry. Various proteins showing altered expression pattern after Fusarium infection were linked to stress response such as plant signal transduction pathways, fungal defense and oxidative burst. More proteins changed during early grain ripening stages than during later ripening stages. Protease inhibitors occurred at increased abundancy during milk ripe stage. A thaumatin-like protein accumulated at plant death stage. Proteins linked to nitrogen metabolism and protein biosynthesis were mainly reduced, whereas those linked to carbon metabolism were predominantly increased in infected grains."],["dc.identifier.doi","10.1016/j.jprot.2015.11.015"],["dc.identifier.pmid","26612662"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/15683"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.issn","1876-7737"],["dc.title","Identification of regulated proteins in naked barley grains (Hordeum vulgare nudum) after Fusarium graminearum infection at different grain ripening stages"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","429"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Toxins"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Ezekiel, Chibundu N."],["dc.contributor.author","Ortega-Beltran, Alejandro"],["dc.contributor.author","Oyedeji, Eniola O."],["dc.contributor.author","Atehnkeng, Joseph"],["dc.contributor.author","Kössler, Philip"],["dc.contributor.author","Tairu, Folasade"],["dc.contributor.author","Hoeschle-Zeledon, Irmgard"],["dc.contributor.author","Karlovsky, Petr"],["dc.contributor.author","Cotty, Peter J."],["dc.contributor.author","Bandyopadhyay, Ranajit"],["dc.date.accessioned","2020-12-10T18:47:24Z"],["dc.date.available","2020-12-10T18:47:24Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.3390/toxins11070429"],["dc.identifier.eissn","2072-6651"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16779"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78750"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Aflatoxin in Chili Peppers in Nigeria: Extent of Contamination and Control Using Atoxigenic Aspergillus flavus Genotypes as Biocontrol Agents"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]