Zeug, André

[["dc.bibliographiccitation.artnumber","014022"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Journal of Biomedical Optics"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Weigel, Arwed"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Zeug, Andre"],["dc.date.accessioned","2018-11-07T08:34:48Z"],["dc.date.available","2018-11-07T08:34:48Z"],["dc.date.issued","2009"],["dc.description.abstract","The essential feature of the confocal laser scanning microscope (cLSM) is the generation of optical sections by the removal of out-of-focus light. About ten years ago, structured illumination microscopy (SIM) was introduced as an alternative method for obtaining optical sections from biological specimens. Here we compare the resolution of the ApoTome (commercial SIM by Zeiss) to that achieved by a cLSM (Zeiss LSM 510). If fluorescent beads are used as test objects, then the ApoTome will achieve a lower axial resolution than the cLSM. In contrast to that, its lateral resolution scores slightly better. If subresolution homogeneous fluorescent layers are used as test objects, then the ApoTome will achieve a higher axial resolution than the cLSM. The ApoTome's axial resolution is homogeneous over the field-of-view while that of the cLSM changes markedly. Finally, the anisotropy of the ApoTome's resolution was found to be negligible for standard applications while its capability to resolve fine structures within stained tissue slices is limited to one or two cell layers and thus worse than in the cLSM. (C) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3083439]"],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft"],["dc.identifier.doi","10.1117/1.3083439"],["dc.identifier.isi","000264551900027"],["dc.identifier.pmid","19256710"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7767"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17904"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Spie-soc Photoptical Instrumentation Engineers"],["dc.relation.issn","1083-3668"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Resolution in the ApoTome and the confocal laser scanning microscope: comparison"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Gorinski, Nataliya"],["dc.contributor.author","Bijata, Monika"],["dc.contributor.author","Prasad, Sonal"],["dc.contributor.author","Wirth, Alexander"],["dc.contributor.author","Abdel Galil, Dalia"],["dc.contributor.author","Zeug, Andre"],["dc.contributor.author","Bazovkina, Daria"],["dc.contributor.author","Kondaurova, Elena"],["dc.contributor.author","Kulikova, Elizabeth"],["dc.contributor.author","Ilchibaeva, Tatiana"],["dc.contributor.author","Zareba-Koziol, Monika"],["dc.contributor.author","Papaleo, Francesco"],["dc.contributor.author","Scheggia, Diego"],["dc.contributor.author","Kochlamazashvili, Gaga"],["dc.contributor.author","Dityatev, Alexander"],["dc.contributor.author","Smyth, Ian"],["dc.contributor.author","Krzystyniak, Adam"],["dc.contributor.author","Wlodarczyk, Jakub"],["dc.contributor.author","Richter, Diethelm W."],["dc.contributor.author","Strekalova, Tatyana"],["dc.contributor.author","Sigrist, Stephan"],["dc.contributor.author","Bang, Claudia"],["dc.contributor.author","Hobuß, Lisa"],["dc.contributor.author","Fiedler, Jan"],["dc.contributor.author","Thum, Thomas"],["dc.contributor.author","Naumenko, Vladimir S."],["dc.contributor.author","Pandey, Ghanshyam"],["dc.contributor.author","Ponimaskin, Evgeni"],["dc.date.accessioned","2020-12-10T18:09:50Z"],["dc.date.available","2020-12-10T18:09:50Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1038/s41467-019-11876-5"],["dc.identifier.eissn","2041-1723"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16768"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/73772"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Attenuated palmitoylation of serotonin receptor 5-HT1A affects receptor function and contributes to depression-like behaviors"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Journal of Cell Science"],["dc.contributor.author","Renner, Ute"],["dc.contributor.author","Zeug, Andre"],["dc.contributor.author","Woehler, Andrew"],["dc.contributor.author","Niebert, Markus"],["dc.contributor.author","Dityatev, Alexander"],["dc.contributor.author","Dityateva, Galina"],["dc.contributor.author","Gorinski, Nataliya"],["dc.contributor.author","Guseva, Daria"],["dc.contributor.author","Abdel-Galil, Dalia"],["dc.contributor.author","Fröhlich, Matthias"],["dc.contributor.author","Ponimaskin, Evgeni G."],["dc.date.accessioned","2022-03-01T11:44:05Z"],["dc.date.available","2022-03-01T11:44:05Z"],["dc.date.issued","2012"],["dc.description.abstract","Serotonin receptors 5-HT1A and 5-HT7 are highly co-expressed in brain regions implicated in depression. However, their functional interaction has not been established. In the present study we show that 5-HT1A and 5-HT7 receptors form heterodimers both in vitro and in vivo. Foerster resonance energy transfer-based assays revealed that, in addition to heterodimers, homodimers composed either by 5-HT1A or 5-HT7 receptors together with monomers co-exist in cells. The highest affinity to form the complex was obtained for the 5-HT7-5-HT7 homodimers, followed by the 5-HT7-5-HT1A heterodimers and 5-HT1A-5-HT1A homodimers. Functionally, heterodimerization decreases 5-HT1A receptor-mediated activation of Gi-protein without affecting 5-HT7 receptor-mediated signalling. Moreover, heterodimerization markedly decreases the ability of the 5-HT1A receptor to activate G-protein gated inwardly rectifying potassium channels in a heterologous system. The inhibitory effect on such channels was also preserved in hippocampal neurons, demonstrating a physiological relevance of heteromerization in vivo. In addition, heterodimerization is critically involved in initiation of the serotonin-mediated 5-HT1A receptor internalization and also enhances the ability of the 5-HT1A receptor to activate the mitogen-activated protein kinases. Finally, we found that production of 5-HT7 receptors in hippocampus continuously decreases during postnatal development, indicating that the relative concentration of 5-HT1A-5-HT7 heterodimers and, consequently, their functional importance undergoes pronounced developmental changes."],["dc.description.abstract","Serotonin receptors 5-HT1A and 5-HT7 are highly co-expressed in brain regions implicated in depression. However, their functional interaction has not been established. In the present study we show that 5-HT1A and 5-HT7 receptors form heterodimers both in vitro and in vivo. Foerster resonance energy transfer-based assays revealed that, in addition to heterodimers, homodimers composed either by 5-HT1A or 5-HT7 receptors together with monomers co-exist in cells. The highest affinity to form the complex was obtained for the 5-HT7-5-HT7 homodimers, followed by the 5-HT7-5-HT1A heterodimers and 5-HT1A-5-HT1A homodimers. Functionally, heterodimerization decreases 5-HT1A receptor-mediated activation of Gi-protein without affecting 5-HT7 receptor-mediated signalling. Moreover, heterodimerization markedly decreases the ability of the 5-HT1A receptor to activate G-protein gated inwardly rectifying potassium channels in a heterologous system. The inhibitory effect on such channels was also preserved in hippocampal neurons, demonstrating a physiological relevance of heteromerization in vivo. In addition, heterodimerization is critically involved in initiation of the serotonin-mediated 5-HT1A receptor internalization and also enhances the ability of the 5-HT1A receptor to activate the mitogen-activated protein kinases. Finally, we found that production of 5-HT7 receptors in hippocampus continuously decreases during postnatal development, indicating that the relative concentration of 5-HT1A-5-HT7 heterodimers and, consequently, their functional importance undergoes pronounced developmental changes."],["dc.identifier.doi","10.1242/jcs.101337"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/102922"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.eissn","1477-9137"],["dc.relation.issn","0021-9533"],["dc.title","Heterodimerization of serotonin receptors 5-HT1A and 5-HT7 differentially regulates receptor signalling and trafficking"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","049801"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Journal of Biomedical Optics"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Weigel, Arwed"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Zeug, Andre"],["dc.date.accessioned","2018-11-07T08:57:10Z"],["dc.date.available","2018-11-07T08:57:10Z"],["dc.date.issued","2011"],["dc.identifier.doi","10.1117/1.3585832"],["dc.identifier.isi","000291031400038"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/23327"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Spie-soc Photoptical Instrumentation Engineers"],["dc.relation.issn","1083-3668"],["dc.title","Resolution in the ApoTome and the confocal laser scanning microscope: comparison (vol 14, 014022, 2009)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3791"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","3800"],["dc.bibliographiccitation.volume","96"],["dc.contributor.author","Neher, Richard A."],["dc.contributor.author","Mitkovski, Miso"],["dc.contributor.author","Kirchhoff, Frank"],["dc.contributor.author","Neher, Erwin"],["dc.contributor.author","Theis, Fabian J."],["dc.contributor.author","Zeug, Andre"],["dc.date.accessioned","2018-11-07T08:30:00Z"],["dc.date.available","2018-11-07T08:30:00Z"],["dc.date.issued","2009"],["dc.description.abstract","Methods of blind source separation are used in many contexts to separate composite data sets according to their sources. Multiply labeled fluorescence microscopy images represent such sets, in which the sources are the individual labels. Then distributions are the quantities of interest and have to be extracted from the images. This is often challenging, since the recorded emission spectra of fluorescent dyes are environment- and instrument-specific. We have developed a nonnegative matrix factorization (NMF) algorithm to detect and separate spectrally distinct components of multiply labeled fluorescence images. It operates on spectrally resolved images and delivers both the emission spectra of the identified components and images of their abundance. We tested the proposed method using biological samples labeled with up to four spectrally overlapping fluorescent labels. In most cases, NMF accurately decomposed the images into contributions of individual dyes. However, the Solutions are not unique when spectra overlap strongly or when images are diffuse in their structure. To arrive at satisfactory results in such cases, we extended NMF to incorporate preexisting qualitative knowledge about spectra and label distributions. We show how data acquired through excitations at two or three different wavelengths can be integrated and that multiple excitations greatly facilitate the decomposition. By allowing reliable decomposition in cases where the spectra of the individual labels are not known or are known only inaccurately, the proposed algorithms greatly extend the range of questions that can be addressed with quantitative microscopy."],["dc.identifier.doi","10.1016/j.bpj.2008.10.068"],["dc.identifier.isi","000266397700033"],["dc.identifier.pmid","19413985"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7763"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/16787"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.relation.issn","0006-3495"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Blind Source Separation Techniques for the Decomposition of Multiply Labeled Fluorescence Images"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","367"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Glia"],["dc.bibliographiccitation.lastpage","376"],["dc.bibliographiccitation.volume","58"],["dc.contributor.author","Ribes, Sandra"],["dc.contributor.author","Ebert, Sandra"],["dc.contributor.author","Regen, Tommy"],["dc.contributor.author","Czesnik, Dirk"],["dc.contributor.author","Scheffel, Joerg"],["dc.contributor.author","Zeug, Andre"],["dc.contributor.author","Bunkowski, Stephanie"],["dc.contributor.author","Eiffert, Helmut"],["dc.contributor.author","Hanisch, Uwe-Karsten"],["dc.contributor.author","Hammerschmidt, Sven"],["dc.contributor.author","Nau, Roland"],["dc.date.accessioned","2018-11-07T08:46:15Z"],["dc.date.available","2018-11-07T08:46:15Z"],["dc.date.issued","2010"],["dc.description.abstract","Microglia express Toll-like receptors (TLRs) that recognize invading pathogens as well as endogenous proteins such as fibronectin under nonphysiological conditions. Here, we demonstrated that fibronectin stimulates murine microglia in culture in a dose-dependent manner: microglial cells secreted proinflammatory cytokines and chemokines and increased phagocytosis of Escherichia coli DH5 alpha and E. coli K1 strains. Low levels of fibronectin exerted a synergistic effect on the release of proinflammatory compounds by microglia co-stimulated with agonists for TLR1/2 (Pam(3)CSK(4)) or TLR9 (CpG DNA), but not in combination with the TLR4 agonist lipopolysaccharide (LPS). Phagocytosis of bacterial strains was moderately enhanced when microglia was co-stimulated with high concentrations of fibronectin and one pathogen-derived TLR agonist. In conclusion, fibronectin increased proinflammatory and phagocytotic functions in microglia and partially synergized with microbial TLR agonists. (C) 2009 Wiley-Liss, Inc."],["dc.identifier.doi","10.1002/glia.20929"],["dc.identifier.isi","000273189600009"],["dc.identifier.pmid","19780198"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/20644"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-liss"],["dc.relation.issn","0894-1491"],["dc.title","Fibronectin Stimulates Escherichia coli Phagocytosis by Microglial Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1752"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Molecular Psychiatry"],["dc.bibliographiccitation.lastpage","1767"],["dc.bibliographiccitation.volume","21"],["dc.contributor.author","Hassouna, I."],["dc.contributor.author","Ott, C."],["dc.contributor.author","Wüstefeld, L."],["dc.contributor.author","Offen, N."],["dc.contributor.author","Neher, R. A."],["dc.contributor.author","Mitkovski, M."],["dc.contributor.author","Winkler, D."],["dc.contributor.author","Sperling, S."],["dc.contributor.author","Fries, L."],["dc.contributor.author","Goebbels, S."],["dc.contributor.author","Vreja, I. C."],["dc.contributor.author","Hagemeyer, N."],["dc.contributor.author","Dittrich, M."],["dc.contributor.author","Rossetti, M. F."],["dc.contributor.author","Kröhnert, K."],["dc.contributor.author","Hannke, K."],["dc.contributor.author","Boretius, S."],["dc.contributor.author","Zeug, A."],["dc.contributor.author","Höschen, C."],["dc.contributor.author","Dandekar, T."],["dc.contributor.author","Dere, E."],["dc.contributor.author","Neher, E."],["dc.contributor.author","Rizzoli, S. O."],["dc.contributor.author","Nave, K.-A."],["dc.contributor.author","Sirén, A.-L."],["dc.contributor.author","Ehrenreich, H."],["dc.date.accessioned","2017-09-07T11:53:29Z"],["dc.date.available","2017-09-07T11:53:29Z"],["dc.date.issued","2016"],["dc.description.abstract","Recombinant human erythropoietin (EPO) improves cognitive performance in neuropsychiatric diseases ranging from schizophrenia and multiple sclerosis to major depression and bipolar disease. This consistent EPO effect on cognition is independent of its role in hematopoiesis. The cellular mechanisms of action in brain, however, have remained unclear. Here we studied healthy young mice and observed that 3-week EPO administration was associated with an increased number of pyramidal neurons and oligodendrocytes in the hippocampus of ~20%. Under constant cognitive challenge, neuron numbers remained elevated until >6 months of age. Surprisingly, this increase occurred in absence of altered cell proliferation or apoptosis. After feeding a 15N-leucine diet, we used nanoscopic secondary ion mass spectrometry, and found that in EPO-treated mice, an equivalent number of neurons was defined by elevated 15N-leucine incorporation. In EPO-treated NG2-Cre-ERT2 mice, we confirmed enhanced differentiation of preexisting oligodendrocyte precursors in the absence of elevated DNA synthesis. A corresponding analysis of the neuronal lineage awaits the identification of suitable neuronal markers. In cultured neurospheres, EPO reduced Sox9 and stimulated miR124, associated with advanced neuronal differentiation. We are discussing a resulting working model in which EPO drives the differentiation of non-dividing precursors in both (NG2+) oligodendroglial and neuronal lineages. As endogenous EPO expression is induced by brain injury, such a mechanism of adult neurogenesis may be relevant for central nervous system regeneration."],["dc.identifier.doi","10.1038/mp.2015.212"],["dc.identifier.fs","620695"],["dc.identifier.gro","3145088"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14225"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2785"],["dc.language.iso","en"],["dc.notes.intern","Crossref Import"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation.issn","1359-4184"],["dc.rights","CC BY-NC-ND 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-nd/4.0"],["dc.title","Revisiting adult neurogenesis and the role of erythropoietin for neuronal and oligodendroglial differentiation in the hippocampus"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","5412"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","5423"],["dc.bibliographiccitation.volume","95"],["dc.contributor.author","Salonikidis, Petrus S."],["dc.contributor.author","Zeug, Andre"],["dc.contributor.author","Kobe, Fritz"],["dc.contributor.author","Ponimaskin, Evgeni G."],["dc.contributor.author","Richter, Diethelm W."],["dc.date.accessioned","2018-11-07T11:08:21Z"],["dc.date.available","2018-11-07T11:08:21Z"],["dc.date.issued","2008"],["dc.description.abstract","Forster resonance energy transfer (FRET)-based biosensors for the quantitative analysis of intracellular signaling, including sensors for monitoring cyclic adenosine monophosphate (cAMP), are of increasing interest. The measurement of the donor/acceptor emission ratio in tandem biosensors excited at the donor excitation wavelength is a commonly used technique. A general problem, however, is that this ratio varies not only with the changes in cAMP concentration but also with the changes of the ionic environment or other factors affecting the folding probability of the fluorophores. Here, we use a spectral FRET analysis on the basis of two excitation wavelengths to obtain a reliable measure of the absolute cAMP concentrations with high temporal and spatial resolution by using an \"exchange protein directly activated by cAMP\". In this approach, FRET analysis is simplified and does not require additional calibration routines. The change in FRET efficiency (E) of the biosensor caused by [cAMP] changes was determined as Delta E=15%, whereas E varies between 35% at low and 20% at high [cAMP], allowing quantitative measurement of cAMP concentration in the range from 150 nM to 15 mu M. The method described is also suitable for other FRET-based biosensors with a 1: 1 donor/acceptor stoichiometry. As a proof of principle, we measured the specially resolved cAMP concentration within living cells and determined the dynamic changes of cAMP levels after stimulation of the Gs-coupled serotonin receptor subtype 7 (5-HT7)."],["dc.identifier.doi","10.1529/biophysj.107.125666"],["dc.identifier.isi","000260999500039"],["dc.identifier.pmid","18708470"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7766"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52756"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biophysical Soc"],["dc.relation.issn","0006-3495"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Quantitative Measurement of cAMP Concentration Using an Exchange Protein Directly Activated by a cAMP-Based FRET-Sensor"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4865"],["dc.bibliographiccitation.issue","24"],["dc.bibliographiccitation.journal","Human Molecular Genetics"],["dc.bibliographiccitation.lastpage","4878"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Noelle, Anna"],["dc.contributor.author","Zeug, Andre"],["dc.contributor.author","van Bergeijk, Jeroen"],["dc.contributor.author","Toenges, Lars"],["dc.contributor.author","Gerhard, Ralf"],["dc.contributor.author","Brinkmann, Hella"],["dc.contributor.author","Al Rayes, Sarah"],["dc.contributor.author","Hensel, Niko"],["dc.contributor.author","Schill, Yvonne"],["dc.contributor.author","Apkhazava, David"],["dc.contributor.author","Jablonka, Sibylle"],["dc.contributor.author","O'mer, Jana"],["dc.contributor.author","Srivastav, Ratnesh Kumar"],["dc.contributor.author","Baasner, Anne"],["dc.contributor.author","Lingor, Paul"],["dc.contributor.author","Wirth, Brunhilde"],["dc.contributor.author","Ponimaskin, Evgeni G."],["dc.contributor.author","Niedenthal, Rainer"],["dc.contributor.author","Grothe, Claudia"],["dc.contributor.author","Claus, Peter"],["dc.date.accessioned","2018-11-07T08:48:53Z"],["dc.date.available","2018-11-07T08:48:53Z"],["dc.date.issued","2011"],["dc.description.abstract","Spinal muscular atrophy (SMA), a frequent neurodegenerative disease, is caused by reduced levels of functional survival of motoneuron (SMN) protein. SMN is involved in multiple pathways, including RNA metabolism and splicing as well as motoneuron development and function. Here we provide evidence for a major contribution of the Rho-kinase (ROCK) pathway in SMA pathogenesis. Using an in vivo protein interaction system based on SUMOylation of proteins, we found that SMN is directly interacting with profilin2a. Profilin2a binds to a stretch of proline residues in SMN, which is heavily impaired by a novel SMN2 missense mutation (S230L) derived from a SMA patient. In different SMA models, we identified differential phosphorylation of the ROCK-downstream targets cofilin, myosin-light chain phosphatase and profilin2a. We suggest that hyper-phosphorylation of profilin2a is the molecular link between SMN and the ROCK pathway repressing neurite outgrowth in neuronal cells. Finally, we found a neuron-specific increase in the F-/G-actin ratio that further support the role of actin dynamics in SMA pathogenesis."],["dc.identifier.doi","10.1093/hmg/ddr425"],["dc.identifier.isi","000297242100010"],["dc.identifier.pmid","21920940"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/21329"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.relation.issn","0964-6906"],["dc.title","The spinal muscular atrophy disease protein SMN is linked to the rho-kinase pathway via profilin"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1821"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","1827"],["dc.bibliographiccitation.volume","103"],["dc.contributor.author","Zeug, André"],["dc.contributor.author","Woehler, Andrew"],["dc.contributor.author","Neher, Erwin"],["dc.contributor.author","Ponimaskin, Evgeni G."],["dc.date.accessioned","2022-03-01T11:44:56Z"],["dc.date.available","2022-03-01T11:44:56Z"],["dc.date.issued","2012"],["dc.identifier.doi","10.1016/j.bpj.2012.09.031"],["dc.identifier.pii","S0006349512010727"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103164"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.issn","0006-3495"],["dc.title","Quantitative Intensity-Based FRET Approaches—A Comparative Snapshot"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]