Bohnert, Maria

[["dc.bibliographiccitation.firstpage","345"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Trends in Cell Biology"],["dc.bibliographiccitation.lastpage","358"],["dc.bibliographiccitation.volume","31"],["dc.contributor.author","Herker, Eva"],["dc.contributor.author","Vieyres, Gabrielle"],["dc.contributor.author","Beller, Mathias"],["dc.contributor.author","Krahmer, Natalie"],["dc.contributor.author","Bohnert, Maria"],["dc.date.accessioned","2022-07-11T15:39:16Z"],["dc.date.available","2022-07-11T15:39:16Z"],["dc.date.issued","2021"],["dc.description.abstract","After having been disregarded for a long time as inert fat drops, lipid droplets (LDs) are now recognized as ubiquitous cellular organelles with key functions in lipid biology and beyond. The identification of abundant LD contact sites, places at which LDs are physically attached to other organelles, has uncovered an unexpected level of communication between LDs and the rest of the cell. In recent years, many disease factors mutated in hereditary disorders have been recognized as LD contact site proteins. Furthermore, LD contact sites are dramatically rearranged in response to infections with intracellular pathogens, as well as under pathological metabolic conditions such as hepatic steatosis. Collectively, it is emerging that LD-organelle contacts are important players in development and progression of disease."],["dc.identifier.doi","10.1016/j.tcb.2021.01.004"],["dc.identifier.pmid","33546922"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112471"],["dc.identifier.url","https://sfb1190.med.uni-goettingen.de/production/literature/publications/137"],["dc.language.iso","en"],["dc.relation","SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente"],["dc.relation","SFB 1190 | P21: Funktionelle Rolle der LDO-Maschinerie in Lipidkörper-Organell Kontaktstellen"],["dc.relation.eissn","1879-3088"],["dc.relation.issn","0962-8924"],["dc.relation.workinggroup","RG Bohnert (Lipid Droplet Kommunikation)"],["dc.rights","CC BY-NC-ND 4.0"],["dc.title","Lipid Droplet Contact Sites in Health and Disease"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dc.type.subtype","overview_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","103512"],["dc.bibliographiccitation.journal","Insect Biochemistry and Molecular Biology"],["dc.bibliographiccitation.volume","133"],["dc.contributor.author","Chartschenko, Eugenia"],["dc.contributor.author","Hugenroth, Marie"],["dc.contributor.author","Akhtar, Irfan"],["dc.contributor.author","Droste, Andrea"],["dc.contributor.author","Kolkhof, Petra"],["dc.contributor.author","Bohnert, Maria"],["dc.contributor.author","Beller, Mathias"],["dc.date.accessioned","2022-07-11T08:57:47Z"],["dc.date.available","2022-07-11T08:57:47Z"],["dc.date.issued","2021"],["dc.description.abstract","The Seipin protein is a conserved key component in the biogenesis of lipid droplets (LDs). Recently, a cooperation between human Seipin and the Lipid droplet assembly factor 1 (LDAF1) was described. LDAF1 physically interacts with Seipin and the holocomplex safeguards regular LD biogenesis. The function of LDAF1 proteins outside mammals is less clear. In yeast, the lipid droplet organization (LDO) proteins, which also cooperate with Seipin, are the putative homologs of LDAF1. While certain functional aspects are shared between the LDO and mammalian LDAF1 proteins, the relationship between the proteins is under debate. Here, we identify the Drosophila melanogaster protein CG32803, which we re-named to dmLDAF1, as an insect member of this protein family. dmLDAF1 decorates LDs in cultured cells and in vivo and the protein is linked to the fly and mouse Seipin proteins. Altering the dmLDAF1 abundance affects LD size, number and overall lipid storage amounts. Our results suggest that the LDAF1 proteins thus fulfill an evolutionarily conserved function in the biogenesis and biology of LDs."],["dc.identifier.doi","10.1016/j.ibmb.2020.103512"],["dc.identifier.pmid","33307187"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112455"],["dc.identifier.url","https://sfb1190.med.uni-goettingen.de/production/literature/publications/130"],["dc.language.iso","en"],["dc.relation","SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente"],["dc.relation","SFB 1190 | P21: Funktionelle Rolle der LDO-Maschinerie in Lipidkörper-Organell Kontaktstellen"],["dc.relation.eissn","1879-0240"],["dc.relation.issn","0965-1748"],["dc.relation.workinggroup","RG Bohnert (Lipid Droplet Kommunikation)"],["dc.title","CG32803 is the fly homolog of LDAF1 and influences lipid storage in vivo"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","269"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","The Journal of Cell Biology"],["dc.bibliographiccitation.lastpage","282"],["dc.bibliographiccitation.volume","217"],["dc.contributor.author","Eisenberg-Bord, Michal"],["dc.contributor.author","Mari, Muriel"],["dc.contributor.author","Weill, Uri"],["dc.contributor.author","Rosenfeld-Gur, Eden"],["dc.contributor.author","Moldavski, Ofer"],["dc.contributor.author","Castro, Inês G."],["dc.contributor.author","Soni, Krishnakant G."],["dc.contributor.author","Harpaz, Nofar"],["dc.contributor.author","Levine, Tim P"],["dc.contributor.author","Futerman, Anthony H."],["dc.contributor.author","Reggiori, Fulvio"],["dc.contributor.author","Bankaitis, Vytas A."],["dc.contributor.author","Schuldiner, Maya"],["dc.contributor.author","Bohnert, Maria"],["dc.date.accessioned","2022-07-04T12:38:21Z"],["dc.date.available","2022-07-04T12:38:21Z"],["dc.date.issued","2018"],["dc.description.abstract","Functional heterogeneity within the lipid droplet (LD) pool of a single cell has been observed, yet the underlying mechanisms remain enigmatic. Here, we report on identification of a specialized LD subpopulation characterized by a unique proteome and a defined geographical location at the nucleus-vacuole junction contact site. In search for factors determining identity of these LDs, we screened ∼6,000 yeast mutants for loss of targeting of the subpopulation marker Pdr16 and identified Ldo45 (LD organization protein of 45 kD) as a crucial targeting determinant. Ldo45 is the product of a splicing event connecting two adjacent genes (YMR147W and YMR148W/OSW5/LDO16). We show that Ldo proteins cooperate with the LD biogenesis component seipin and establish LD identity by defining positioning and surface-protein composition. Our studies suggest a mechanism to establish functional differentiation of organelles, opening the door to better understanding of metabolic decisions in cells."],["dc.identifier.doi","10.1083/jcb.201704122"],["dc.identifier.pmid","29187527"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112374"],["dc.identifier.url","https://sfb1190.med.uni-goettingen.de/production/literature/publications/17"],["dc.language.iso","en"],["dc.relation","SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente"],["dc.relation","SFB 1190 | P11: Zuordnung zellulärer Kontaktstellen und deren Zusammenspiel"],["dc.relation.eissn","1540-8140"],["dc.relation.issn","0021-9525"],["dc.relation.workinggroup","RG Bohnert (Lipid Droplet Kommunikation)"],["dc.relation.workinggroup","RG Schuldiner (Functional Genomics of Organelles)"],["dc.rights","CC BY 4.0"],["dc.title","Identification of seipin-linked factors that act as determinants of a lipid droplet subpopulation"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","483"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Nature Reviews. Molecular Cell Biology"],["dc.bibliographiccitation.lastpage","484"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Bohnert, Maria"],["dc.contributor.author","Schuldiner, Maya"],["dc.date.accessioned","2022-07-11T15:20:59Z"],["dc.date.available","2022-07-11T15:20:59Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1038/s41580-018-0022-1"],["dc.identifier.pmid","29765158"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112466"],["dc.identifier.url","https://sfb1190.med.uni-goettingen.de/production/literature/publications/88"],["dc.language.iso","en"],["dc.relation","SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente"],["dc.relation","SFB 1190 | P11: Zuordnung zellulärer Kontaktstellen und deren Zusammenspiel"],["dc.relation.eissn","1471-0080"],["dc.relation.issn","1471-0072"],["dc.relation.workinggroup","RG Schuldiner (Functional Genomics of Organelles)"],["dc.relation.workinggroup","RG Bohnert (Lipid Droplet Kommunikation)"],["dc.title","Stepping outside the comfort zone of membrane contact site research"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","overview_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","395"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Developmental Cell"],["dc.bibliographiccitation.lastpage","409"],["dc.bibliographiccitation.volume","39"],["dc.contributor.author","Eisenberg-Bord, Michal"],["dc.contributor.author","Shai, Nadav"],["dc.contributor.author","Schuldiner, Maya"],["dc.contributor.author","Bohnert, Maria"],["dc.date.accessioned","2022-07-11T15:02:22Z"],["dc.date.available","2022-07-11T15:02:22Z"],["dc.date.issued","2016"],["dc.description.abstract","Membrane contact sites enable interorganelle communication by positioning organelles in close proximity using molecular \"tethers.\" With a growing understanding of the importance of contact sites, the hunt for new contact sites and their tethers is in full swing. Determining just what is a tether has proven challenging. Here, we aim to delineate guidelines that define the prerequisites for categorizing a protein as a tether. Setting this gold standard now, while groups from different disciplines are beginning to explore membrane contact sites, will enable efficient cooperation in the growing field and help to realize a great collaborative opportunity to boost its development."],["dc.identifier.doi","10.1016/j.devcel.2016.10.022"],["dc.identifier.pmid","27875684"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112461"],["dc.identifier.url","https://sfb1190.med.uni-goettingen.de/production/literature/publications/4"],["dc.language.iso","en"],["dc.relation","SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente"],["dc.relation","SFB 1190 | P11: Zuordnung zellulärer Kontaktstellen und deren Zusammenspiel"],["dc.relation.eissn","1878-1551"],["dc.relation.issn","1534-5807"],["dc.relation.workinggroup","RG Bohnert (Lipid Droplet Kommunikation)"],["dc.relation.workinggroup","RG Schuldiner (Functional Genomics of Organelles)"],["dc.title","A Tether Is a Tether Is a Tether: Tethering at Membrane Contact Sites"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","overview_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","212"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Developmental Cell"],["dc.bibliographiccitation.lastpage","225"],["dc.bibliographiccitation.volume","54"],["dc.contributor.author","Bohnert, Maria"],["dc.date.accessioned","2022-07-11T15:34:17Z"],["dc.date.available","2022-07-11T15:34:17Z"],["dc.date.issued","2020"],["dc.description.abstract","Membrane contact sites (CSs) are specialized cellular regions where distinct organelles are actively positioned very close to each other, at a distance of just a few nanometers. Structurally, CS formation depends on tether proteins that physically link organellar membranes to each other. Functionally, these structures act as hotspots for communication and material transfer. In recent years, we are starting to understand that the cellular CS landscape is not static but instead responds dynamically to diverse metabolic cues. This review describes the interplay between cellular metabolism and CS-based organelle communication and discusses molecular mechanisms of contact modulation in cellular adaptation to changing metabolic requirements."],["dc.identifier.doi","10.1016/j.devcel.2020.06.026"],["dc.identifier.pmid","32693056"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112469"],["dc.identifier.url","https://sfb1190.med.uni-goettingen.de/production/literature/publications/122"],["dc.language.iso","en"],["dc.relation","SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente"],["dc.relation","SFB 1190 | P21: Funktionelle Rolle der LDO-Maschinerie in Lipidkörper-Organell Kontaktstellen"],["dc.relation.eissn","1878-1551"],["dc.relation.issn","1534-5807"],["dc.relation.workinggroup","RG Bohnert (Lipid Droplet Kommunikation)"],["dc.title","Tether Me, Tether Me Not-Dynamic Organelle Contact Sites in Metabolic Rewiring"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","overview_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1188"],["dc.bibliographiccitation.issue","10, Pt. B"],["dc.bibliographiccitation.journal","Biochimica et Biophysica Acta. Molecular and Cell Biology of Lipids"],["dc.bibliographiccitation.lastpage","1196"],["dc.bibliographiccitation.volume","1862"],["dc.contributor.author","Schuldiner, Maya"],["dc.contributor.author","Bohnert, Maria"],["dc.date.accessioned","2022-07-11T15:51:36Z"],["dc.date.available","2022-07-11T15:51:36Z"],["dc.date.issued","2017-10"],["dc.description.abstract","Lipid droplets (LDs) store lipids and hence serve as energy reservoir and as a source for building-blocks for the organelle membrane systems. LD biology therefore depends on tight communication with other organelles. The unique architecture of LDs, consisting of a neutral lipid core shielded by a phospholipid-monolayer, is however an obstacle to bulk-exchange of bilayer-bounded vesicles with other organelles. In recent years, it is emerging that contact sites, places where two organelles are positioned in close proximity allowing vesicle-independent communication, are an important way to integrate LDs into the organellar landscape. However, few LD contact sites have been studied in depth and our understanding of their structure, extent and function is only starting to emerge. Here, we highlight recent findings on the functions of LD contact sites and on the proteins involved in their formation and hypothesize about the unique characteristics of the contact sites formed by these intriguing organelles. This article is part of a Special Issue entitled: Recent Advances in Lipid Droplet Biology edited by Rosalind Coleman and Matthijs Hesselink."],["dc.identifier.doi","10.1016/j.bbalip.2017.06.005"],["dc.identifier.pmid","28627434"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112473"],["dc.identifier.url","https://sfb1190.med.uni-goettingen.de/production/literature/publications/27"],["dc.language.iso","en"],["dc.relation","SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente"],["dc.relation","SFB 1190 | P11: Zuordnung zellulärer Kontaktstellen und deren Zusammenspiel"],["dc.relation.issn","1388-1981"],["dc.relation.workinggroup","RG Bohnert (Lipid Droplet Kommunikation)"],["dc.relation.workinggroup","RG Schuldiner (Functional Genomics of Organelles)"],["dc.rights","CC BY-NC-ND 4.0"],["dc.title","A different kind of love - lipid droplet contact sites"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dc.type.subtype","overview_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2836"],["dc.bibliographiccitation.issue","13"],["dc.bibliographiccitation.journal","Cell Reports"],["dc.bibliographiccitation.lastpage","2852"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Morgenstern, Marcel"],["dc.contributor.author","Stiller, Sebastian B."],["dc.contributor.author","Lübbert, Philipp"],["dc.contributor.author","Peikert, Christian D."],["dc.contributor.author","Dannenmaier, Stefan"],["dc.contributor.author","Drepper, Friedel"],["dc.contributor.author","Weill, Uri"],["dc.contributor.author","Höß, Philipp"],["dc.contributor.author","Feuerstein, Reinhild"],["dc.contributor.author","Gebert, Michael"],["dc.contributor.author","Bohnert, Maria"],["dc.contributor.author","van der Laan, Martin"],["dc.contributor.author","Schuldiner, Maya"],["dc.contributor.author","Schütze, Conny"],["dc.contributor.author","Oeljeklaus, Silke"],["dc.contributor.author","Pfanner, Nikolaus"],["dc.contributor.author","Wiedemann, Nils"],["dc.contributor.author","Warscheid, Bettina"],["dc.date.accessioned","2022-07-06T07:42:11Z"],["dc.date.available","2022-07-06T07:42:11Z"],["dc.date.issued","2017"],["dc.description.abstract","Mitochondria perform central functions in cellular bioenergetics, metabolism, and signaling, and their dysfunction has been linked to numerous diseases. The available studies cover only part of the mitochondrial proteome, and a separation of core mitochondrial proteins from associated fractions has not been achieved. We developed an integrative experimental approach to define the proteome of yeast mitochondria. We classified > 3,300 proteins of mitochondria and mitochondria-associated fractions and defined 901 high-confidence mitochondrial proteins, expanding the set of mitochondrial proteins by 82. Our analysis includes protein abundance under fermentable and nonfermentable growth, submitochondrial localization, single-protein experiments, and subcellular classification of mitochondria-associated fractions. We identified mitochondrial interactors of respiratory chain supercomplexes, ATP synthase, AAA proteases, the mitochondrial contact site and cristae organizing system (MICOS), and the coenzyme Q biosynthesis cluster, as well as mitochondrial proteins with dual cellular localization. The integrative proteome provides a high-confidence source for the characterization of physiological and pathophysiological functions of mitochondria and their integration into the cellular environment."],["dc.identifier.doi","10.1016/j.celrep.2017.06.014"],["dc.identifier.pmid","28658629"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112399"],["dc.identifier.url","https://sfb1190.med.uni-goettingen.de/production/literature/publications/92"],["dc.language.iso","en"],["dc.relation","SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente"],["dc.relation","SFB 1190 | P11: Zuordnung zellulärer Kontaktstellen und deren Zusammenspiel"],["dc.relation.eissn","2211-1247"],["dc.relation.workinggroup","RG Bohnert (Lipid Droplet Kommunikation)"],["dc.relation.workinggroup","RG Schuldiner (Functional Genomics of Organelles)"],["dc.rights","CC BY-NC-ND 4.0"],["dc.title","Definition of a High-Confidence Mitochondrial Proteome at Quantitative Scale"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","268"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Cells"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Castro, Inês G."],["dc.contributor.author","Eisenberg-Bord, Michal"],["dc.contributor.author","Persiani, Elisa"],["dc.contributor.author","Rochford, Justin J."],["dc.contributor.author","Schuldiner, Maya"],["dc.contributor.author","Bohnert, Maria"],["dc.date.accessioned","2022-07-04T14:24:51Z"],["dc.date.available","2022-07-04T14:24:51Z"],["dc.date.issued","2019"],["dc.description.abstract","Seipin (BSCL2/SPG17) is a key factor in lipid droplet (LD) biology, and its dysfunction results in severe pathologies, including the fat storage disease Berardinelli-Seip congenital lipodystrophy type 2, as well as several neurological seipinopathies. Despite its importance for human health, the molecular role of seipin is still enigmatic. Seipin is evolutionarily conserved from yeast to humans. In yeast, seipin was recently found to cooperate with the lipid droplet organization (LDO) proteins, Ldo16 and Ldo45, two structurally-related proteins involved in LD function and identity that display remote homology to the human protein promethin/TMEM159. In this study, we show that promethin is indeed an LD-associated protein that forms a complex with seipin, and its localization to the LD surface can be modulated by seipin expression levels. We thus identify promethin as a novel seipin partner protein."],["dc.identifier.doi","10.3390/cells8030268"],["dc.identifier.pmid","30901948"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112383"],["dc.identifier.url","https://sfb1190.med.uni-goettingen.de/production/literature/publications/62"],["dc.language.iso","en"],["dc.relation","SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente"],["dc.relation","SFB 1190 | P11: Zuordnung zellulärer Kontaktstellen und deren Zusammenspiel"],["dc.relation.issn","2073-4409"],["dc.relation.workinggroup","RG Bohnert (Lipid Droplet Kommunikation)"],["dc.relation.workinggroup","RG Schuldiner (Functional Genomics of Organelles)"],["dc.rights","CC BY 4.0"],["dc.title","Promethin Is a Conserved Seipin Partner Protein"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]