Beta, Carsten

[["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","PMC Biophysics"],["dc.bibliographiccitation.lastpage","15"],["dc.bibliographiccitation.volume","3"],["dc.contributor.author","Westendorf, Christian"],["dc.contributor.author","Bae, Albert J."],["dc.contributor.author","Erlenkamper, Christoph"],["dc.contributor.author","Galland, Edouard"],["dc.contributor.author","Franck, Carl"],["dc.contributor.author","Bodenschatz, Eberhard"],["dc.contributor.author","Beta, Carsten"],["dc.date.accessioned","2019-07-09T11:52:49Z"],["dc.date.available","2019-07-09T11:52:49Z"],["dc.date.issued","2010"],["dc.description.abstract","Eukaryotic cell flattening is valuable for improving microscopic observations, ranging from bright field (BF) to total internal reflection fluorescence (TIRF) microscopy. Fundamental processes, such as mitosis and in vivo actin polymerization, have been investigated using these techniques. Here, we review the well known agar overlayer protocol and the oil overlay method. In addition, we present more elaborate microfluidics-based techniques that provide us with a greater level of control. We demonstrate these techniques on the social amoebae Dictyostelium discoideum, comparing the advantages and disadvantages of each method. PACS Codes: 87.64.-t, 47.61.-k, 87.80.Ek"],["dc.identifier.doi","10.1186/1757-5036-3-9"],["dc.identifier.pmid","20403171"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6028"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60285"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.orgunit","Fakultät für Physik"],["dc.rights","Goescholar"],["dc.rights.uri","https://goedoc.uni-goettingen.de/licenses"],["dc.subject.ddc","530"],["dc.title","Live cell flattening -traditional and novel approaches"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","14"],["dc.bibliographiccitation.journal","Physical Review Letters"],["dc.bibliographiccitation.volume","119"],["dc.contributor.author","Hsu, Hsin-Fang"],["dc.contributor.author","Bodenschatz, Eberhard"],["dc.contributor.author","Westendorf, Christian"],["dc.contributor.author","Gholami, Azam"],["dc.contributor.author","Pumir, Alain"],["dc.contributor.author","Tarantola, Marco"],["dc.contributor.author","Beta, Carsten"],["dc.date.accessioned","2020-12-10T18:25:44Z"],["dc.date.available","2020-12-10T18:25:44Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1103/PhysRevLett.119.148101"],["dc.identifier.eissn","1079-7114"],["dc.identifier.issn","0031-9007"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/75806"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Variability and Order in Cytoskeletal Dynamics of Motile Amoeboid Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3059"],["dc.bibliographiccitation.issue","21"],["dc.bibliographiccitation.journal","Lab on a Chip"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Bae, Albert J."],["dc.contributor.author","Beta, Carsten"],["dc.contributor.author","Bodenschatz, Eberhard"],["dc.date.accessioned","2022-06-08T07:58:43Z"],["dc.date.available","2022-06-08T07:58:43Z"],["dc.date.issued","2009"],["dc.identifier.doi","10.1039/b905521e"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/110506"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-575"],["dc.relation.eissn","1473-0189"],["dc.relation.issn","1473-0197"],["dc.title","Rapid switching of chemical signals in microfluidic devices"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Physical Review Letters"],["dc.bibliographiccitation.volume","109"],["dc.contributor.author","Amselem, Gabriel"],["dc.contributor.author","Theves, Matthias"],["dc.contributor.author","Bae, Albert"],["dc.contributor.author","Beta, Carsten"],["dc.contributor.author","Bodenschatz, Eberhard"],["dc.date.accessioned","2022-06-08T07:59:58Z"],["dc.date.available","2022-06-08T07:59:58Z"],["dc.date.issued","2012"],["dc.identifier.doi","10.1103/PhysRevLett.109.108103"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/110922"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-575"],["dc.relation.eissn","1079-7114"],["dc.relation.issn","0031-9007"],["dc.title","Control Parameter Description of Eukaryotic Chemotaxis"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","28005"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","EPL (Europhysics Letters)"],["dc.bibliographiccitation.volume","90"],["dc.contributor.author","Bödeker, H. U."],["dc.contributor.author","Beta, C."],["dc.contributor.author","Frank, T. D."],["dc.contributor.author","Bodenschatz, E."],["dc.date.accessioned","2022-06-08T07:57:22Z"],["dc.date.available","2022-06-08T07:57:22Z"],["dc.date.issued","2010"],["dc.identifier.doi","10.1209/0295-5075/90/28005"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/110067"],["dc.notes.intern","DOI-Import GROB-575"],["dc.relation.eissn","1286-4854"],["dc.relation.issn","0295-5075"],["dc.title","Quantitative analysis of random ameboid motion"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3853"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"],["dc.bibliographiccitation.lastpage","3858"],["dc.bibliographiccitation.volume","110"],["dc.contributor.author","Westendorf, Christian"],["dc.contributor.author","Negrete, Jose, Jr."],["dc.contributor.author","Bae, Albert J."],["dc.contributor.author","Sandmann, Rabea"],["dc.contributor.author","Bodenschatz, Eberhard"],["dc.contributor.author","Beta, Carsten"],["dc.date.accessioned","2018-11-07T09:27:08Z"],["dc.date.available","2018-11-07T09:27:08Z"],["dc.date.issued","2013"],["dc.description.abstract","The rapid reorganization of the actin cytoskeleton in response to external stimuli is an essential property of many motile eukaryotic cells. Here, we report evidence that the actin machinery of chemotactic Dictyostelium cells operates close to an oscillatory instability. When averaging the actin response of many cells to a short pulse of the chemoattractant cAMP, we observed a transient accumulation of cortical actin reminiscent of a damped oscillation. At the single-cell level, however, the response dynamics ranged from short, strongly damped responses to slowly decaying, weakly damped oscillations. Furthermore, in a small subpopulation, we observed self-sustained oscillations in the cortical F-actin concentration. To substantiate that an oscillatory mechanism governs the actin dynamics in these cells, we systematically exposed a large number of cells to periodic pulse trains of different frequencies. Our results indicate a resonance peak at a stimulation period of around 20 s. We propose a delayed feedback model that explains our experimental findings based on a time-delay in the regulatory network of the actin system. To test the model, we performed stimulation experiments with cells that express GFP-tagged fusion proteins of Coronin and actin-interacting protein 1, as well as knockout mutants that lack Coronin and actin-interacting protein 1. These actin-binding proteins enhance the disassembly of actin filaments and thus allow us to estimate the delay time in the regulatory feedback loop. Based on this independent estimate, our model predicts an intrinsic period of 20 s, which agrees with the resonance observed in our periodic stimulation experiments."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft [1128, 937]"],["dc.identifier.doi","10.1073/pnas.1216629110"],["dc.identifier.isi","000316377400046"],["dc.identifier.pmid","23431176"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/30465"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Natl Acad Sciences"],["dc.relation.issn","0027-8424"],["dc.title","Actin cytoskeleton of chemotactic amoebae operates close to the onset of oscillations"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","083015"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","New Journal of Physics"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Beta, C"],["dc.contributor.author","Amselem, G"],["dc.contributor.author","Bodenschatz, E."],["dc.date.accessioned","2022-06-08T07:58:58Z"],["dc.date.available","2022-06-08T07:58:58Z"],["dc.date.issued","2008"],["dc.identifier.doi","10.1088/1367-2630/10/8/083015"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/110589"],["dc.notes.intern","DOI-Import GROB-575"],["dc.relation.eissn","1367-2630"],["dc.title","A bistable mechanism for directional sensing"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Physical Review Applied"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Lepro, Valentino"],["dc.contributor.author","Großmann, Robert"],["dc.contributor.author","Sharifi Panah, Setareh"],["dc.contributor.author","Nagel, Oliver"],["dc.contributor.author","Klumpp, Stefan"],["dc.contributor.author","Lipowsky, Reinhard"],["dc.contributor.author","Beta, Carsten"],["dc.date.accessioned","2022-11-01T10:16:58Z"],["dc.date.available","2022-11-01T10:16:58Z"],["dc.date.issued","2022"],["dc.description.sponsorship"," Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659"],["dc.description.sponsorship"," IMPRS 501100012318"],["dc.identifier.doi","10.1103/PhysRevApplied.18.034014"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/116700"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-605"],["dc.relation.eissn","2331-7019"],["dc.rights.uri","https://link.aps.org/licenses/aps-default-license"],["dc.title","Optimal Cargo Size for Active Diffusion of Biohybrid Microcarriers"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","811"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","European Journal of Cell Biology"],["dc.bibliographiccitation.lastpage","816"],["dc.bibliographiccitation.volume","90"],["dc.contributor.author","Beta, Carsten"],["dc.contributor.author","Bodenschatz, Eberhard"],["dc.date.accessioned","2022-06-08T07:57:49Z"],["dc.date.available","2022-06-08T07:57:49Z"],["dc.date.issued","2011"],["dc.identifier.doi","10.1016/j.ejcb.2011.05.006"],["dc.identifier.pii","S0171933511001038"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/110225"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-575"],["dc.relation.issn","0171-9335"],["dc.title","Microfluidic tools for quantitative studies of eukaryotic chemotaxis"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3940"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Analytical Chemistry"],["dc.bibliographiccitation.lastpage","3944"],["dc.bibliographiccitation.volume","79"],["dc.contributor.author","Beta, Carsten"],["dc.contributor.author","Wyatt, Danica"],["dc.contributor.author","Rappel, Wouter-Jan"],["dc.contributor.author","Bodenschatz, Eberhard"],["dc.date.accessioned","2022-06-08T07:58:38Z"],["dc.date.available","2022-06-08T07:58:38Z"],["dc.date.issued","2007"],["dc.identifier.doi","10.1021/ac070033y"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/110477"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-575"],["dc.relation.eissn","1520-6882"],["dc.relation.issn","0003-2700"],["dc.title","Flow Photolysis for Spatiotemporal Stimulation of Single Cells"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]