Thiele, Jan Christoph

[["dc.bibliographiccitation.firstpage","38"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Communications Biology"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Oleksiievets, Nazar"],["dc.contributor.author","Sargsyan, Yelena"],["dc.contributor.author","Thiele, Jan Christoph"],["dc.contributor.author","Mougios, Nikolaos"],["dc.contributor.author","Sograte-Idrissi, Shama"],["dc.contributor.author","Nevskyi, Oleksii"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Opazo, Felipe"],["dc.contributor.author","Thoms, Sven"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Tsukanov, Roman"],["dc.date.accessioned","2022-01-13T06:27:32Z"],["dc.date.available","2022-01-13T06:27:32Z"],["dc.date.issued","2022-01-11"],["dc.description.abstract","DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) is a powerful super-resolution technique highly suitable for multi-target (multiplexing) bio-imaging. However, multiplexed imaging of cells is still challenging due to the dense and sticky environment inside a cell. Here, we combine fluorescence lifetime imaging microscopy (FLIM) with DNA-PAINT and use the lifetime information as a multiplexing parameter for targets identification. In contrast to Exchange-PAINT, fluorescence lifetime PAINT (FL-PAINT) can image multiple targets simultaneously and does not require any fluid exchange, thus leaving the sample undisturbed and making the use of flow chambers/microfluidic systems unnecessary. We demonstrate the potential of FL-PAINT by simultaneous imaging of up to three targets in a cell using both wide-field FLIM and 3D time-resolved confocal laser scanning microscopy (CLSM). FL-PAINT can be readily combined with other existing techniques of multiplexed imaging and is therefore a perfect candidate for high-throughput multi-target bio-imaging."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2022"],["dc.identifier.doi","10.1038/s42003-021-02976-4"],["dc.identifier.pmid","35017652"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/98096"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/392"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/415"],["dc.language.iso","en"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A10: Peroxisomen als modulatorische Einheiten im Herzstoffwechsel und bei Herzinsuffizienz"],["dc.relation.issn","2399-3642"],["dc.relation.orgunit","III. Physikalisches Institut - Biophysik"],["dc.relation.workinggroup","RG Enderlein"],["dc.relation.workinggroup","RG Thoms (Biochemistry and Molecular Medicine)"],["dc.rights","CC BY 4.0"],["dc.title","Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","32"],["dc.bibliographiccitation.journal","Methods"],["dc.bibliographiccitation.lastpage","39"],["dc.bibliographiccitation.volume","140-141"],["dc.contributor.author","Ghosh, Arindam"],["dc.contributor.author","Karedla, Narain"],["dc.contributor.author","Thiele, Jan Christoph"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2020-12-10T15:21:49Z"],["dc.date.available","2020-12-10T15:21:49Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1016/j.ymeth.2018.02.009"],["dc.identifier.issn","1046-2023"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/73174"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation.orgunit","III. Physikalisches Institut - Biophysik"],["dc.title","Fluorescence lifetime correlation spectroscopy: Basics and applications"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.contributor.author","Thiele, Jan Christoph"],["dc.contributor.author","Jungblut, Marvin"],["dc.contributor.author","Helmerich, Dominic A."],["dc.contributor.author","Tsukanov, Roman"],["dc.contributor.author","Chizhik, Anna M."],["dc.contributor.author","Chizhik, Alexey I."],["dc.contributor.author","Schnermann, Martin"],["dc.contributor.author","Sauer, Markus"],["dc.contributor.author","Nevskyi, Oleksii"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2022-01-12T16:50:43Z"],["dc.date.available","2022-01-12T16:50:43Z"],["dc.date.issued","2021-12-22"],["dc.description.abstract","Over the last two decades, super-resolution microscopy has seen a tremendous development in speed and resolution, but for most of its methods, there exists a remarkable gap between lateral and axial resolution. Similar to conventional optical microscopy, the axial resolution is by a factor three to five worse than the lateral resolution. One recently developed method to close this gap is metal-induced energy transfer (MIET) imaging which achieves an axial resolution down to nanometers. It exploits the distance dependent quenching of fluorescence when a fluorescent molecule is brought close to a metal surface. In the present manuscript, we combine the extreme axial resolution of MIET imaging with the extraordinary lateral resolution of single-molecule localization microscopy, in particular with direct stochastic optical reconstruction microscopy (dSTORM). This combination allows us to achieve isotropic three-dimensional super-resolution imaging of sub-cellular structures. Moreover, we employed spectral demixing for implementing dualcolor MIET-dSTORM that allows us to image and co-localize, in three dimensions, two different cellular structures simultaneously."],["dc.format.extent","16"],["dc.identifier.doi","10.1101/2021.12.20.473473"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/98094"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/378"],["dc.language.iso","en"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.orgunit","III. Physikalisches Institut - Biophysik"],["dc.relation.workinggroup","RG Enderlein"],["dc.title","Isotropic Three-Dimensional Dual-Color Super-Resolution Microscopy with Metal-Induced Energy Transfer"],["dc.type","preprint"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","740281"],["dc.bibliographiccitation.journal","Frontiers in Bioinformatics"],["dc.bibliographiccitation.volume","1"],["dc.contributor.affiliation","Thiele, Jan Christoph; \r\n\r\n1\r\nThird Institute of Physics—Biophysics, Georg August University, Göttingen, Germany"],["dc.contributor.affiliation","Nevskyi, Oleksii; \r\n\r\n1\r\nThird Institute of Physics—Biophysics, Georg August University, Göttingen, Germany"],["dc.contributor.affiliation","Helmerich, Dominic A.; \r\n\r\n2\r\nDepartment of Biotechnology and Biophysics, Biocenter, Julius-Maximilians-Universität Würzburg, Würzburg, Germany"],["dc.contributor.affiliation","Sauer, Markus; \r\n\r\n2\r\nDepartment of Biotechnology and Biophysics, Biocenter, Julius-Maximilians-Universität Würzburg, Würzburg, Germany"],["dc.contributor.affiliation","Enderlein, Jörg; \r\n\r\n1\r\nThird Institute of Physics—Biophysics, Georg August University, Göttingen, Germany"],["dc.contributor.author","Thiele, Jan Christoph"],["dc.contributor.author","Nevskyi, Oleksii"],["dc.contributor.author","Helmerich, Dominic A."],["dc.contributor.author","Sauer, Markus"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2022-01-12T16:50:47Z"],["dc.date.available","2022-01-12T16:50:47Z"],["dc.date.issued","2021"],["dc.date.updated","2022-09-04T02:08:32Z"],["dc.description.abstract","Fluorescence-lifetime single molecule localization microscopy (FL-SMLM) adds the lifetime dimension to the spatial super-resolution provided by SMLM. Independent of intensity and spectrum, this lifetime information can be used, for example, to quantify the energy transfer efficiency in Förster Resonance Energy Transfer (FRET) imaging, to probe the local environment with dyes that change their lifetime in an environment-sensitive manner, or to achieve image multiplexing by using dyes with different lifetimes. We present a thorough theoretical analysis of fluorescence-lifetime determination in the context of FL-SMLM and compare different lifetime-fitting approaches. In particular, we investigate the impact of background and noise, and give clear guidelines for procedures that are optimized for FL-SMLM. We do also present and discuss our public-domain software package “Fluorescence-Lifetime TrackNTrace,” which converts recorded fluorescence microscopy movies into super-resolved FL-SMLM images."],["dc.identifier.doi","10.3389/fbinf.2021.740281"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/98095"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/406"],["dc.language.iso","en"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","2673-7647"],["dc.relation.issn","2673-7647"],["dc.relation.orgunit","III. Physikalisches Institut - Biophysik"],["dc.relation.workinggroup","RG Enderlein"],["dc.rights","CC BY 4.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Advanced Data Analysis for Fluorescence-Lifetime Single-Molecule Localization Microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

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[["dc.bibliographiccitation.firstpage","112015"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Physics of Fluids"],["dc.bibliographiccitation.volume","32"],["dc.contributor.author","Thiele, Jan Christoph"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Karedla, Narain"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2021-03-05T08:58:44Z"],["dc.date.available","2021-03-05T08:58:44Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1063/5.0024190"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80228"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/93"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-393"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","1089-7666"],["dc.relation.issn","1070-6631"],["dc.relation.orgunit","III. Physikalisches Institut - Biophysik"],["dc.relation.workinggroup","RG Enderlein"],["dc.title","Efficient modeling of three-dimensional convection–diffusion problems in stationary flows"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.contributor.author","Thiele, Jan Christoph"],["dc.contributor.author","Helmerich, Dominic"],["dc.contributor.author","Oleksiievets, Nazar"],["dc.contributor.author","Tsukanov, Roman"],["dc.contributor.author","Butkevich, Eugenia"],["dc.contributor.author","Sauer, Markus"],["dc.contributor.author","Nevskyi, Oleksii"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2022-01-12T16:50:38Z"],["dc.date.available","2022-01-12T16:50:38Z"],["dc.date.issued","2020"],["dc.description.abstract","Fluorescence lifetime imaging microscopy (FLIM) is an important technique that adds another dimension to the intensity and colour information of conventional microscopy. In particular, it allows for multiplexing fluorescent labels that have otherwise similar spectral properties. Currently, the only super-resolution technique that is capable of recording super-resolved images with lifetime information is STimulated Emission Depletion (STED) microscopy. In contrast, all Single-Molecule Localisation Microscopy (SMLM) techniques that employ wide-field cameras completely lack the lifetime dimension. Here, we combine Fluorescence-Lifetime Confocal Laser-Scanning Microscopy (FL-CLSM) with SMLM for realising single-molecule localisation-based fluorescence-lifetime super-resolution imaging (FL-SMLM). Besides yielding images with a spatial resolution much beyond the diffraction limit, it determines the fluorescence lifetime of all localised molecules. We validate our technique by applying it to direct STochastic Optical Reconstruction Microscopy (dSTORM) and Points Accumulation for Imaging in Nanoscale Topography (PAINT) imaging of fixed cells, and we demonstrate its multiplexing capability on samples with two different labels that differ only by fluorescence lifetime but not by their spectral properties."],["dc.format.extent","30"],["dc.identifier.doi","10.1101/2020.08.25.266387"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/98093"],["dc.language.iso","en"],["dc.relation.orgunit","III. Physikalisches Institut - Biophysik"],["dc.title","Confocal Laser-Scanning Fluorescence-Lifetime Single-Molecule Localisation Microscopy"],["dc.type","preprint"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

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