Alves, Frauke

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Svetlove, Angelika"],["dc.contributor.author","Zschüntzsch, Jana"],["dc.contributor.author","Alves, Frauke"],["dc.date.accessioned","2022-09-01T09:50:08Z"],["dc.date.available","2022-09-01T09:50:08Z"],["dc.date.issued","2022"],["dc.description.abstract","Abstract\n \n Retrospective gating (RG) is a well established technique in preclinical computed tomography (CT) to assess 3D morphology of the lung. In RG additional angular projections are recorded typically by performing multiple rotations. Consequently, the projections are sorted according to the expansion state of the chest and those sets are then reconstructed separately. Thus, the breathing motion artefacts are suppressed at a cost of strongly elevated X-ray dose levels. Here we propose to use the entire raw data to assess respiratory motion in addition to retrospectively gated 3D reconstruction that visualize anatomical structures of the lung. Using this RG based X-ray respiratory motion measurement approach, which will be referred to as RG based X-ray lung function measurement (rgXLF) on the example of the\n mdx\n mouse model of Duchenne muscle dystrophy (mdx) we accurately obtained both the 3D anatomical morphology of the lung and the thoracic bones as well as functional temporal parameters of the lung. Thus, rgXLF will remove the necessity for separate acquisition procedures by being able to reproduce comparable results to the previously established planar X-ray based lung function measurement approach in a single low dose CT scan."],["dc.identifier.doi","10.1038/s41598-022-17335-4"],["dc.identifier.pii","17335"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/113630"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-597"],["dc.relation.eissn","2045-2322"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Simultaneous assessment of lung morphology and respiratory motion in retrospectively gated in-vivo microCT of free breathing anesthetized mice"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","619"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Nature Nanotechnology"],["dc.bibliographiccitation.lastpage","+"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Kreyling, Wolfgang G."],["dc.contributor.author","Abdelmonem, Abuelmagd M."],["dc.contributor.author","Ali, Zulqurnain"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Geiser, Marianne"],["dc.contributor.author","Haberl, Nadine"],["dc.contributor.author","Hartmann, Raimo"],["dc.contributor.author","Hirn, Stephanie"],["dc.contributor.author","Jimenez de Aberasturi, Dorleta"],["dc.contributor.author","Kantner, Karsten"],["dc.contributor.author","Khadem-Saba, Guelnaz"],["dc.contributor.author","Montenegro, Jose-Maria"],["dc.contributor.author","Rejman, Joanna"],["dc.contributor.author","Rojo, Teofilo"],["dc.contributor.author","Ruiz de Larramendi, Idoia"],["dc.contributor.author","Ufartes, Roser"],["dc.contributor.author","Wenk, Alexander"],["dc.contributor.author","Parak, Wolfgang J."],["dc.date.accessioned","2018-11-07T09:55:19Z"],["dc.date.available","2018-11-07T09:55:19Z"],["dc.date.issued","2015"],["dc.description.abstract","Inorganic nanoparticles are frequently engineered with an organic surface coating to improve their physicochemical properties, and it is well known that their colloidal properties(1) may change upon internalization by cells(2,3). While the stability of such nanoparticles is typically assayed in simple in vitro tests, their stability in a mammalian organism remains unknown. Here, we show that firmly grafted polymer shells around gold nanoparticles may degrade when injected into rats. We synthesized monodisperse radioactively labelled gold nanoparticles (Au-198)(4) and engineered an In-111-labelled polymer shell around them(5). Upon intravenous injection into rats, quantitative biodistribution analyses performed independently for Au-198 and In-111 showed partial removal of the polymer shell in vivo. While Au-198 accumulates mostly in the liver, part of the In-111 shows a non-particulate biodistribution similar to intravenous injection of chelated In-111. Further in vitro studies suggest that degradation of the polymer shell is caused by proteolytic enzymes in the liver. Our results show that even nanoparticles with high colloidal stability can change their physicochemical properties in vivo."],["dc.identifier.doi","10.1038/NNANO.2015.111"],["dc.identifier.isi","000357485600017"],["dc.identifier.pmid","26076469"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36714"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","1748-3395"],["dc.relation.issn","1748-3387"],["dc.title","In vivo integrity of polymer-coated gold nanoparticles"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2540"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","International Journal of Cancer"],["dc.bibliographiccitation.lastpage","2552"],["dc.bibliographiccitation.volume","139"],["dc.contributor.author","Giannuzzo, Andrea"],["dc.contributor.author","Saccomano, Mara"],["dc.contributor.author","Napp, Joanna"],["dc.contributor.author","Ellegaard, Maria"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Novak, Ivana"],["dc.date.accessioned","2018-11-07T10:05:36Z"],["dc.date.available","2018-11-07T10:05:36Z"],["dc.date.issued","2016"],["dc.description.abstract","The ATP-gated receptor P2X7 (P2X7R) is involved in regulation of cell survival and has been of interest in cancer field. Pancreatic ductal adenocarcinoma (PDAC) is a deadly cancer and new markers and therapeutic targets are needed. PDAC is characterized by a complex tumour microenvironment, which includes cancer and pancreatic stellate cells (PSCs), and potentially high nucleotide/side turnover. Our aim was to determine P2X7R expression and function in human pancreatic cancer cells in vitro as well as to perform in vivo efficacy study applying P2X7R inhibitor in an orthotopic xenograft mouse model of PDAC. In the in vitro studies we show that human PDAC cells with luciferase gene (PancTu-1 Luc cells) express high levels of P2X7R protein. Allosteric P2X7R antagonist AZ10606120 inhibited cell proliferation in basal conditions, indicating that P2X7R was tonically active. Extracellular ATP and BzATP, to which the P2X7R is more sensitive, further affected cell survival and confirmed complex functionality of P2X7R. PancTu-1 Luc migration and invasion was reduced by AZ10606120, and it was stimulated by PSCs, but not by PSCs from P2X7(-/-) animals. PancTu-1 Luc cells were orthotopically transplanted into nude mice and tumour growth was followed noninvasively by bioluminescence imaging. AZ10606120-treated mice showed reduced bioluminescence compared to saline-treated mice. Immunohistochemical analysis confirmed P2X7R expression in cancer and PSC cells, and in metaplastic/neoplastic acinar and duct structures. PSCs number/activity and collagen deposition was reduced in AZ10606120-treated tumours. What's new? Pancreatic ductal adenocarcinoma (PDAC) is one the most difficult types of cancer to detect and treat, challenges that could be overcome through the discovery and development of novel markers and therapeutic strategies. Here, the P2X7 receptor, which regulates cell survival, is shown to also support cell proliferation, migration and invasion in human P2X7R-expressing PDAC cells. Treatment of orthotopic PDAC tumor-bearing mice with the P2X7R-specific inhibitor, AZ10606120, resulted in decreased tumor bioluminescence and reductions in pancreatic stellate cells and collagen deposition. Targeting of P2X7R warrants further investigation as a promising therapeutic approach in pancreatic cancer."],["dc.identifier.doi","10.1002/ijc.30380"],["dc.identifier.isi","000384646200017"],["dc.identifier.pmid","27513892"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13821"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38927"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","1097-0215"],["dc.relation.issn","0020-7136"],["dc.rights","CC BY-NC-ND 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-nd/4.0"],["dc.title","Targeting of the P2X7 receptor in pancreatic cancer and stellate cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","andr.13292"],["dc.bibliographiccitation.firstpage","1660"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Andrology"],["dc.bibliographiccitation.lastpage","1672"],["dc.bibliographiccitation.volume","10"],["dc.contributor.affiliation","Pinkert‐Leetsch, Diana; 1\r\nDepartment of Diagnostic and Interventional Radiology\r\nUniversity Medical Center Goettingen\r\nGoettingen Germany"],["dc.contributor.affiliation","Rost, John Uwe; 1\r\nDepartment of Diagnostic and Interventional Radiology\r\nUniversity Medical Center Goettingen\r\nGoettingen Germany"],["dc.contributor.affiliation","Schmiedeknecht, Max Ulrich Heiner; 3\r\nDepartment of Neuropathology\r\nUniversity Medical Center Goettingen\r\nGoettingen Germany"],["dc.contributor.affiliation","Stadelmann, Christine; 3\r\nDepartment of Neuropathology\r\nUniversity Medical Center Goettingen\r\nGoettingen Germany"],["dc.contributor.affiliation","Alves, Frauke; 1\r\nDepartment of Diagnostic and Interventional Radiology\r\nUniversity Medical Center Goettingen\r\nGoettingen Germany"],["dc.contributor.author","Pinkert‐Leetsch, Diana"],["dc.contributor.author","Rost, John Uwe"],["dc.contributor.author","Schmiedeknecht, Max Ulrich Heiner"],["dc.contributor.author","Stadelmann, Christine"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Missbach‐Guentner, Jeannine"],["dc.date.accessioned","2022-11-28T08:48:04Z"],["dc.date.available","2022-11-28T08:48:04Z"],["dc.date.issued","2022-09-15"],["dc.date.updated","2022-11-27T10:10:46Z"],["dc.description.abstract","Abstract\r\n\r\nBackground\r\nThe unique anatomy of the male reproductive organ reflects its complex function from sperm maturation to their storage for months until emission. Since light microscopy in two dimensions (2d) cannot sufficiently demonstrate its complex morphology, a comprehensive visualization is required to identify pathologic alterations in its entire anatomical context.\r\n\r\n\r\nObjectives\r\nAim of this study was to use three‐dimensional (3d) light sheet fluorescence microscopy (LSFM) to visualize entire murine testes in 3d, label‐free and at subcellular resolution, and to assign local autofluorescence to testicular and deferent structures.\r\n\r\n\r\nMaterials and methods\r\nMurine testes were fixed with four different fixatives and subsequently cleared with benzoic acid/benzyl benzoate. Hereafter, complete murine testes were scanned with LSFM with different fluorescence filter sets and subsequently embedded in paraffin for further conventional planar histology.\r\n\r\n\r\nResults\r\nAutofluorescence signals of the murine reproductive organ allowed the unambiguous identification of the testicular anatomy from the seminiferous tubules to the vas deferens with their specific stratification independent of the used fixative. Blood vessels were visualized from the pampiniform plexus to the small capillaries of single tubules. Moreover, due to the specific intrinsic fluorescence properties of the efferent ducts and the epididymis, luminal caliber, the epithelial stratification and retronuclear cytoplasmic inclusions gave a unique insight into the interface of both morphological structures. Subsequent 2d histology confirmed the identified morphological structures.\r\n\r\n\r\nDiscussion\r\nLSFM analysis of the murine reproductive organ allows due to its intrinsic fluorescence a simple, label‐free 3d assessment of its entire duct morphology, the epithelial composition, and the associated blood supply in its anatomical relation.\r\n\r\n\r\nConclusion\r\nLSFM provides the technical basis for comprehensive analyses of pathologically altered murine testes in its entirety by depicting specific autofluorescence. Thereby it facilitates mouse studies of testicular disease or their drug‐related alterations in more detail potentially for clinical translation assessing human testicular biopsies."],["dc.description.sponsorship","Bundesministerium fuer Bildung und Forschung, Deutschland"],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659"],["dc.identifier.doi","10.1111/andr.13292"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/117280"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-600"],["dc.relation.eissn","2047-2927"],["dc.relation.issn","2047-2919"],["dc.rights","This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made."],["dc.title","The murine male reproductive organ at a glance: Three‐dimensional insights and virtual histology using label‐free light sheet microcopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","1600253"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","BioEssays"],["dc.bibliographiccitation.volume","39"],["dc.contributor.author","Pedersen, Stine F."],["dc.contributor.author","Novak, Ivana"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Schwab, Albrecht"],["dc.contributor.author","Pardo, Luis A."],["dc.date.accessioned","2018-10-10T13:25:55Z"],["dc.date.available","2018-10-10T13:25:55Z"],["dc.date.issued","2017"],["dc.description.abstract","We present here the hypothesis that the unique microenvironmental pH landscape of acid-base transporting epithelia is an important factor in development of epithelial cancers, by rendering the epithelial and stromal cells pre-adapted to the heterogeneous extracellular pH (pHe ) in the tumor microenvironment. Cells residing in organs with net acid-base transporting epithelia such as the pancreatic ductal and gastric epithelia are exposed to very different, temporally highly variable pHe values apically and basolaterally. This translates into spatially and temporally non-uniform intracellular pH (pHi ) patterns. Disturbed pHe - and pHi -homeostasis contributes to essentially all hallmarks of cancer. Our hypothesis, that the physiological pHe microenvironment in acid-base secreting epithelia shapes cancers arising in these tissues, can be tested using novel imaging tools. The acidic tumor pHe in turn might be exploited therapeutically. Pancreatic cancers are used as our prime example, but we propose that this concept is also relevant for other cancers of acid-base transporting epithelia."],["dc.fs.pkfprnr","66887"],["dc.identifier.doi","10.1002/bies.201600253"],["dc.identifier.fs","631974"],["dc.identifier.pmid","28440551"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/15964"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1521-1878"],["dc.title","Alternating pH landscapes shape epithelial cancer initiation and progression: Focus on pancreatic cancer"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Pharmacology"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Hartung, Franziska"],["dc.contributor.author","Krüwel, Thomas"],["dc.contributor.author","Shi, Xiaoyi"],["dc.contributor.author","Pfizenmaier, Klaus"],["dc.contributor.author","Kontermann, Roland"],["dc.contributor.author","Chames, Patrick"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Pardo, Luis A."],["dc.date.accessioned","2021-04-14T08:26:27Z"],["dc.date.available","2021-04-14T08:26:27Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.3389/fphar.2020.00686"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/81946"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1663-9812"],["dc.title","A Novel Anti-Kv10.1 Nanobody Fused to Single-Chain TRAIL Enhances Apoptosis Induction in Cancer Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nanoscale Research Letters"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Nifontova, Galina"],["dc.contributor.author","Zvaigzne, Maria"],["dc.contributor.author","Baryshnikova, Maria"],["dc.contributor.author","Korostylev, Evgeny"],["dc.contributor.author","Ramos-Gomes, Fernanda"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Nabiev, Igor"],["dc.contributor.author","Sukhanova, Alyona"],["dc.date.accessioned","2020-12-10T18:38:49Z"],["dc.date.available","2020-12-10T18:38:49Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1186/s11671-018-2447-z"],["dc.identifier.eissn","1556-276X"],["dc.identifier.issn","1931-7573"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15484"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15187"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/77446"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Next-Generation Theranostic Agents Based on Polyelectrolyte Microcapsules Encoded with Semiconductor Nanocrystals: Development and Functional Characterization"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1163"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Journal of Synchrotron Radiation"],["dc.bibliographiccitation.lastpage","1172"],["dc.bibliographiccitation.volume","24"],["dc.contributor.author","Nicolas, Jan-David"],["dc.contributor.author","Bernhardt, Marten"],["dc.contributor.author","Markus, Andrea"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Burghammer, Manfred"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2018-04-23T11:48:57Z"],["dc.date.available","2018-04-23T11:48:57Z"],["dc.date.issued","2017"],["dc.description.abstract","A scanning X-ray diffraction study of cardiac tissue has been performed, covering the entire cross section of a mouse heart slice. To this end, moderate focusing by compound refractive lenses to micrometer spot size, continuous scanning, data acquisition by a fast single-photon-counting pixel detector, and fully automated analysis scripts have been combined. It was shown that a surprising amount of structural data can be harvested from such a scan, evaluating the local scattering intensity, interfilament spacing of the muscle tissue, the filament orientation, and the degree of anisotropy. The workflow of data analysis is described and a data analysis toolbox with example data for general use is provided. Since many cardiomyopathies rely on the structural integrity of the sarcomere, the contractile unit of cardiac muscle cells, the present study can be easily extended to characterize tissue from a diseased heart."],["dc.identifier.doi","10.1107/s1600577517011936"],["dc.identifier.gro","3142464"],["dc.identifier.pii","S1600577517011936"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13614"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.status","final"],["dc.relation.eissn","1600-5775"],["dc.relation.issn","1600-5775"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights.uri","http://journals.iucr.org/services/copyrightpolicy.html"],["dc.subject.gro","x-ray scattering"],["dc.title","Scanning X-ray diffraction on cardiac tissue: automatized data analysis and processing"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Journal of Investigative Dermatology"],["dc.bibliographiccitation.volume","130"],["dc.contributor.author","Hippe, Andreas"],["dc.contributor.author","Schorr, Anne"],["dc.contributor.author","Mueller-Homey, Anja"],["dc.contributor.author","Seeliger, Stefan"],["dc.contributor.author","Jannasch, Katharina"],["dc.contributor.author","Buhren, Bettina Alexandra"],["dc.contributor.author","Sleeman, Jonathan"],["dc.contributor.author","Stoecklein, Nikolas H."],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Hoffmann, Thomas"],["dc.contributor.author","Homey, Bernhard"],["dc.date.accessioned","2018-11-07T08:39:51Z"],["dc.date.available","2018-11-07T08:39:51Z"],["dc.date.issued","2010"],["dc.format.extent","S51"],["dc.identifier.isi","000281110100300"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/19099"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.publisher.place","New york"],["dc.relation.eventlocation","Helsinki, FINLAND"],["dc.relation.issn","0022-202X"],["dc.title","Tumor derived CCL20 production critically contributes to angiogenesis and tumor progression"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","703"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Optics and Spectroscopy"],["dc.bibliographiccitation.lastpage","707"],["dc.bibliographiccitation.volume","125"],["dc.contributor.author","Sukhanova, A."],["dc.contributor.author","Ramos-Gomes, F."],["dc.contributor.author","Alves, F."],["dc.contributor.author","Chames, P."],["dc.contributor.author","Baty, D."],["dc.contributor.author","Nabiev, I."],["dc.date.accessioned","2020-12-10T18:37:09Z"],["dc.date.available","2020-12-10T18:37:09Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1134/S0030400X18110309"],["dc.identifier.eissn","1562-6911"],["dc.identifier.issn","0030-400X"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/76859"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Advanced Nanotools for Imaging of Solid Tumors and Circulating and Disseminated Cancer Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]