Böhlken-Fascher, Susanne

[["dc.bibliographiccitation.firstpage","351"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Vaccines"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Eltom, Kamal H."],["dc.contributor.author","Althoff, Anna Christina"],["dc.contributor.author","Hansen, Sören"],["dc.contributor.author","Böhlken-Fascher, Susanne"],["dc.contributor.author","Yousif, Ausama"],["dc.contributor.author","El-Sheikh, Hussein A."],["dc.contributor.author","ElWakeel, Ahmed A."],["dc.contributor.author","Elgamal, Mahmoud A."],["dc.contributor.author","Mossa, Hadeer M."],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.contributor.author","Aboul-Soud, Emad A."],["dc.contributor.author","Wolff, Janika"],["dc.contributor.author","Korthase, Christian"],["dc.contributor.author","Hoffmann, Bernd"],["dc.contributor.author","Adam, Nabawia M."],["dc.contributor.author","Abdelaziz, Sanaa A."],["dc.contributor.author","Shalaby, Mohamed A."],["dc.date.accessioned","2021-06-01T09:42:43Z"],["dc.date.available","2021-06-01T09:42:43Z"],["dc.date.issued","2021"],["dc.description.abstract","The genus capripoxvirus (CaPV), family Poxviridae, includes three virus species: goatpox virus (GPV), sheeppox virus (SPV) and lumpy skin disease virus (LSDV). CaPV causes disease outbreaks with consequent economic losses in Africa and the Middle East. LSDV has recently spread to Southeast Europe. As CaPVs share 96–97% genetic similarity along the length of the entire genome and are difficult to distinguish using serological assays, simple, reliable and fast methods for diagnosis and species differentiation are crucial in cases of disease outbreak. The present study aimed to develop a field-applicable CaPV differentiation method. Nanopore technology was used for whole genome sequencing. A local database of complete CaPV genomes and partial sequences of three genes (RPO30, P32 and GPCR) was established for offline Basic Local Alignment Search Tool (BLAST). Specificities of 98.04% in whole genome and 97.86% in RPO30 gene runs were obtained among the three virus species, while other databases were less specific. The total run time was shortened to approximately 2 h. Functionality of the developed procedure was proved by samples with high host background sequences. Reliable differentiation options for the quality and capacity of hardware, and sample quality of suspected cases, were derived from these findings. The whole workflow can be performed rapidly with a mobile suitcase laboratory and mini-computer, allowing application at the point-of-need with limited resource settings."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.3390/vaccines9040351"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85330"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","2076-393X"],["dc.relation.orgunit","Abteilung Mikrobiologie und Tierhygiene"],["dc.rights","CC BY 4.0"],["dc.title","Differentiation of Capripox Viruses by Nanopore Sequencing"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2627"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Analytical Chemistry"],["dc.bibliographiccitation.lastpage","2634"],["dc.bibliographiccitation.volume","93"],["dc.contributor.author","El Wahed, Ahmed Abd"],["dc.contributor.author","Patel, Pranav"],["dc.contributor.author","Maier, Melanie"],["dc.contributor.author","Pietsch, Corinna"],["dc.contributor.author","Rüster, Dana"],["dc.contributor.author","Böhlken-Fascher, Susanne"],["dc.contributor.author","Kissenkötter, Jonas"],["dc.contributor.author","Behrmann, Ole"],["dc.contributor.author","Frimpong, Michael"],["dc.contributor.author","Diagne, Moussa Moïse"],["dc.contributor.author","Faye, Martin"],["dc.contributor.author","Dia, Ndongo"],["dc.contributor.author","Shalaby, Mohamed A."],["dc.contributor.author","Amer, Haitham"],["dc.contributor.author","Elgamal, Mahmoud"],["dc.contributor.author","Zaki, Ali"],["dc.contributor.author","Ismail, Ghada"],["dc.contributor.author","Kaiser, Marco"],["dc.contributor.author","Corman, Victor M."],["dc.contributor.author","Niedrig, Matthias"],["dc.contributor.author","Landt, Olfert"],["dc.contributor.author","Faye, Ousmane"],["dc.contributor.author","Sall, Amadou A."],["dc.contributor.author","Hufert, Frank T."],["dc.contributor.author","Truyen, Uwe"],["dc.contributor.author","Liebert, Uwe G."],["dc.contributor.author","Weidmann, Manfred"],["dc.date.accessioned","2021-04-14T08:28:45Z"],["dc.date.available","2021-04-14T08:28:45Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1021/acs.analchem.0c04779"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82697"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1520-6882"],["dc.relation.issn","0003-2700"],["dc.title","Suitcase Lab for Rapid Detection of SARS-CoV-2 Based on Recombinase Polymerase Amplification Assay"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","37"],["dc.bibliographiccitation.journal","International Journal of Infectious Diseases"],["dc.bibliographiccitation.volume","79"],["dc.contributor.author","Hansen, S."],["dc.contributor.author","Faye, O."],["dc.contributor.author","Sanabani, S.S."],["dc.contributor.author","Faye, M."],["dc.contributor.author","Böhlken-Fascher, S."],["dc.contributor.author","Sall, A."],["dc.contributor.author","Bekaert, M."],["dc.contributor.author","Weidmann, M."],["dc.contributor.author","Cherny, C.-P."],["dc.contributor.author","Wahed, A. Abd El"],["dc.date.accessioned","2020-12-10T14:24:36Z"],["dc.date.available","2020-12-10T14:24:36Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1016/j.ijid.2018.11.103"],["dc.identifier.issn","1201-9712"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/72300"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Rapid outbreak identification using point of need nanopore sequencing"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","23"],["dc.bibliographiccitation.journal","Journal of Clinical Virology"],["dc.bibliographiccitation.lastpage","27"],["dc.bibliographiccitation.volume","106"],["dc.contributor.author","Hansen, Sören"],["dc.contributor.author","Faye, Oumar"],["dc.contributor.author","Sanabani, Sabri S."],["dc.contributor.author","Faye, Martin"],["dc.contributor.author","Böhlken-Fascher, Susanne"],["dc.contributor.author","Faye, Ousmane"],["dc.contributor.author","Sall, Amadou A."],["dc.contributor.author","Bekaert, Michaël"],["dc.contributor.author","Weidmann, Manfred"],["dc.contributor.author","Czerny, Claus-Peter"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.date.accessioned","2020-12-10T14:25:03Z"],["dc.date.available","2020-12-10T14:25:03Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1016/j.jcv.2018.07.001"],["dc.identifier.issn","1386-6532"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/72419"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Combination random isothermal amplification and nanopore sequencing for rapid identification of the causative agent of an outbreak"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","117"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Veterinary Sciences"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Roller, Marco"],["dc.contributor.author","Hansen, Sören"],["dc.contributor.author","Böhlken-Fascher, Susanne"],["dc.contributor.author","Knauf-Witzens, Tobias"],["dc.contributor.author","Czerny, Claus-Peter"],["dc.contributor.author","Goethe, Ralph"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.date.accessioned","2021-04-14T08:32:27Z"],["dc.date.available","2021-04-14T08:32:27Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.3390/vetsci7030117"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17560"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83927"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","MDPI"],["dc.relation.eissn","2306-7381"],["dc.rights","CC BY 4.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Molecular and Serological Footprints of Mycobacterium avium Subspecies Infections in Zoo Animals"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","50"],["dc.bibliographiccitation.journal","Journal of Virological Methods"],["dc.bibliographiccitation.lastpage","53"],["dc.bibliographiccitation.volume","263"],["dc.contributor.author","Hansen, Sören"],["dc.contributor.author","Dill, Veronika"],["dc.contributor.author","Shalaby, Mohamed A."],["dc.contributor.author","Eschbaumer, Michael"],["dc.contributor.author","Böhlken-Fascher, Susanne"],["dc.contributor.author","Hoffmann, Bernd"],["dc.contributor.author","Czerny, Claus-Peter"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.date.accessioned","2020-12-10T15:20:09Z"],["dc.date.available","2020-12-10T15:20:09Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1016/j.jviromet.2018.10.020"],["dc.identifier.issn","0166-0934"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/72571"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Serotyping of foot-and-mouth disease virus using oxford nanopore sequencing"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","665"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Parasites & Vectors"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Gunaratna, Gayana"],["dc.contributor.author","Manamperi, Aresha"],["dc.contributor.author","Böhlken-Fascher, Susanne"],["dc.contributor.author","Wickremasinge, Renu"],["dc.contributor.author","Gunawardena, Kithsiri"],["dc.contributor.author","Yapa, Bandujith"],["dc.contributor.author","Pathirana, Nishantha"],["dc.contributor.author","Pathirana, Hasantha"],["dc.contributor.author","Silva, Nilanthi de"],["dc.contributor.author","Sooriyaarachchi, Monica"],["dc.contributor.author","Deerasinghe, Theja"],["dc.contributor.author","Mondal, Dinesh"],["dc.contributor.author","Ranasinghe, Shalindra"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.date.accessioned","2019-07-09T11:49:45Z"],["dc.date.available","2019-07-09T11:49:45Z"],["dc.date.issued","2018"],["dc.description.abstract","Background Leishmaniasis is a disease caused by vector-borne protozoans. In Sri Lanka, the cutaneous form of the disease is predominant, which is usually diagnosed using Giemsa-stained slit skin smear examination and by histology. However, the sensitivity of slit skin smears and histology are reportedly low. Moreover, facilities for the highly sensitive polymerase chain reaction (PCR) are available only in a few highly-equipped parasitology laboratories. Therefore, there is a need for low cost, sensitive and specific screening tests for diagnosis of leishmaniasis at the point of need. Results In this study, a mobile suitcase laboratory applying novel extraction (SpeedXtract) and isothermal amplification and detection (recombinase polymerase amplification assay, RPA) methods were evaluated for the diagnosis of cutaneous leishmaniasis in Sri Lanka. First, the developed assay was applied to three different sample types (punch biopsy, slit skin smears and fine needle aspirates) at a local hospital. The results showed that the 2 mm punch biopsy sample produced the best exponential amplification curve and early fluorescence signal in the RPA assay. Secondly, punch biopsies were collected from 150 suspected cutaneous leishmaniasis cases and screened with SpeedXtract/RPA, RNAlater/PCR and ATL buffer/PCR, in addition to Giemsa-stained slit skin smears. Fifty-seven samples were negative in all detection methods. In total 93 samples were positive with assay sensitivities of 65.5% (SpeedXtract/RPA), 63.4% (RNAlater/PCR) and 92.4% (ATL buffer/PCR). The Giemsa-stained slit skin smear delivered the worst clinical sensitivity (32.2%). Conclusions The SpeedXtract/RPA method under field conditions took 35 min, while almost 8 h were needed to finalize the extraction and detection by PCR in the laboratory. The SpeedXtract/RPA method produced similar sensitivity to samples preserved in RNAlater and subjected to PCR amplification, but both were less sensitive than ATL-preserved samples subjected to PCR amplification. There is a need for a standardization of sample collection and nucleic acid extraction methods."],["dc.identifier.doi","10.1186/s13071-018-3238-1"],["dc.identifier.pmid","30577826"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15759"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59621"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","BioMed Central"],["dc.relation.orgunit","Fakultät für Agrarwissenschaften"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Evaluation of rapid extraction and isothermal amplification techniques for the detection of Leishmania donovani DNA from skin lesions of suspected cases at the point of need in Sri Lanka"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","123"],["dc.bibliographiccitation.lastpage","136"],["dc.bibliographiccitation.seriesnr","2142"],["dc.contributor.author","Hansen, Sören"],["dc.contributor.author","Faye, Oumar"],["dc.contributor.author","Sanabani, Sabri S."],["dc.contributor.author","Faye, Martin"],["dc.contributor.author","Böhlken-Fascher, Susanne"],["dc.contributor.author","Faye, Ousmane"],["dc.contributor.author","Sall, Amadou Alpha"],["dc.contributor.author","Bekaert, Michaël"],["dc.contributor.author","Weidmann, Manfred"],["dc.contributor.author","Czerny, Claus-Peter"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.contributor.editor","Kobinger, Gary"],["dc.contributor.editor","Racine, Trina"],["dc.date.accessioned","2021-06-02T10:44:21Z"],["dc.date.available","2021-06-02T10:44:21Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1007/978-1-0716-0581-3_11"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/87005"],["dc.notes.intern","DOI-Import GROB-425"],["dc.publisher","Springer US"],["dc.publisher.place","New York, NY"],["dc.relation.crisseries","Methods in Molecular Biology"],["dc.relation.eisbn","978-1-0716-0581-3"],["dc.relation.isbn","978-1-0716-0580-6"],["dc.relation.ispartof","Methods in Molecular Biology"],["dc.relation.ispartof","Zika Virus : Methods and Protocols"],["dc.relation.ispartofseries","Methods in Molecular Biology; 2142"],["dc.title","Zika Virus Amplification Using Strand Displacement Isothermal Method and Sequencing Using Nanopore Technology"],["dc.type","book_chapter"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","3648"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Hansen, Sören"],["dc.contributor.author","Hotop, Sven-Kevin"],["dc.contributor.author","Faye, Oumar"],["dc.contributor.author","Ndiaye, Oumar"],["dc.contributor.author","Böhlken-Fascher, Susanne"],["dc.contributor.author","Pessôa, Rodrigo"],["dc.contributor.author","Hufert, Frank"],["dc.contributor.author","Stahl-Hennig, Christiane"],["dc.contributor.author","Frank, Ronald"],["dc.contributor.author","Czerny, Claus-Peter"],["dc.contributor.author","Schmidt-Chanasit, Jonas"],["dc.contributor.author","Sanabani, Sabri S."],["dc.contributor.author","Sall, Amadou A."],["dc.contributor.author","Niedrig, Matthias"],["dc.contributor.author","Brönstrup, Mark"],["dc.contributor.author","Fritz, Hans-Joachim"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.date.accessioned","2019-07-09T11:50:13Z"],["dc.date.available","2019-07-09T11:50:13Z"],["dc.date.issued","2019"],["dc.description.abstract","Zika virus (ZIKV) is a mosquito-borne flavivirus. Homologous proteins of different flaviviruses display high degrees of sequence identity, especially within subgroups. This leads to extensive immunological cross-reactivity and corresponding problems for developing a ZIKV-specific serological assay. In this study, peptide microarrays were employed to identify individual ZIKV antibody targets with promise in differential diagnosis. A total of 1643 overlapping oligopeptides were synthesized and printed onto glass slides. Together, they encompass the full amino acid sequences of ZIKV proteomes of African, Brazilian, USA, and French Polynesian origins. The resulting ZIKV scanning microarray chips were used to screen three pools of sera from recent Zika outbreaks in Senegal and Cape Verde, in Brazil, and from overseas travelers returning to the EU. Together with a mixed pool of well characterized, archived sera of patients suffering from infections by dengue, yellow fever, tick-borne encephalitis, and West Nile viruses, a total of 42 sera went into the study. Sixty-eight antibody target regions were identified. Most of which were hitherto unknown. Alignments and sequence comparisons revealed 13 of which could be classified as bona fide ZIKV-specific. These identified antibody target regions constitute a founding set of analytical tools for serological discrimination of ZIKV from other flaviviruses."],["dc.identifier.doi","10.1038/s41598-019-40224-2"],["dc.identifier.pmid","30842564"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15882"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59723"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2045-2322"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","630"],["dc.title","Diagnosing Zika virus infection against a background of other flaviviruses: Studies in high resolution serological analysis."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1392"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Foods"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Kissenkötter, Jonas"],["dc.contributor.author","Böhlken-Fascher, Susanne"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.date.accessioned","2021-04-14T08:31:07Z"],["dc.date.available","2021-04-14T08:31:07Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.3390/foods9101392"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17593"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83492"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","MDPI"],["dc.relation.eissn","2304-8158"],["dc.rights","CC BY 4.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Flesh ID: Nanopore Sequencing Combined with Offline BLAST Search for the Identification of Meat Source"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]