Hoppert, Michael

[["dc.bibliographiccitation.firstpage","355"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","FEMS Microbiology Ecology"],["dc.bibliographiccitation.lastpage","372"],["dc.bibliographiccitation.volume","84"],["dc.contributor.author","Hallmann, Christine"],["dc.contributor.author","Stannek, Lorena"],["dc.contributor.author","Fritzlar, Diana"],["dc.contributor.author","Hause-Reitner, Dorothea"],["dc.contributor.author","Friedl, Thomas"],["dc.contributor.author","Hoppert, Michael"],["dc.date.accessioned","2018-11-07T09:25:36Z"],["dc.date.available","2018-11-07T09:25:36Z"],["dc.date.issued","2013"],["dc.description.abstract","Composition and diversity of aeroterrestrial phototrophic microbial communities are up to now poorly understood. Here, we present a comparative study addressing the composition of algal communities on sandstone substrata based upon the analysis of rRNA gene clone libraries from environmental samples and crude cultures. From a west-facing, shaded wall area of the mediaeval castle ruin Gleichen (Thuringia, Germany), sequences mainly related to the green algae Prasiococcus and Trebouxia (Trebouxiophyceae) were retrieved. A south-west-facing, sun-exposed wall area was mainly colonized by Apatococcus and a Phyllosiphon-related alga. Just a few species, in particular Stichococcus-related strains, were ubiquitous in both areas. Samples from a basement vault exposed to low irradiance exhibited Chlorophyceae like Chromochloris and Bracteacoccus. Thus, most green algae on the daylight-exposed walls were affiliated to Trebouxiophyceae, whereas Chlorophyceae were dominant in samples taken from the site kept under low irradiance. Accordingly, cyanobacterial communities were different: the sun-exposed area was dominated by Synechococcus-related organisms, while on the shaded wall area, cyanobacteria were almost absent. The filamentous Leptolyngbya dominated samples from the basement vault. Scanning electron microscopy revealed endolithic algal morphotypes (coccoid algae and diatoms) dominant in open pores between mineral particles. Here, the organisms may be also involved in biogenic weathering of stone."],["dc.identifier.doi","10.1111/1574-6941.12065"],["dc.identifier.isi","000317430200012"],["dc.identifier.pmid","23278436"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/30101"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","0168-6496"],["dc.title","Molecular diversity of phototrophic biofilms on building stone"],["dc.type","review"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2411"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Journal of Bacteriology"],["dc.bibliographiccitation.lastpage","2421"],["dc.bibliographiccitation.volume","190"],["dc.contributor.author","Jakovljevic, Vladimir"],["dc.contributor.author","Leonardy, Simone"],["dc.contributor.author","Hoppert, Michael"],["dc.contributor.author","Sogaard-Andersen, Lotte"],["dc.date.accessioned","2018-11-07T11:16:36Z"],["dc.date.available","2018-11-07T11:16:36Z"],["dc.date.issued","2008"],["dc.description.abstract","Type IV pili (T4P) are dynamic surface structures that undergo cycles of extension and retraction. T4P dynamics center on the PilB and PilT proteins, which are members of the secretion ATPase superfamily of proteins. Here, we show that PilB and PilT of the T4P system in Myxococcus xanthus have ATPase activity in vitro. Using a structure-guided approach, we systematically mutagenized PilB and PilT to resolve whether both ATP binding and hydrolysis are important for PilB and PilT function in vivo. PilB as well as PilT ATPase activity was abolished in vitro by replacement of conserved residues in the Walker A and Walker B boxes that are involved in ATP binding and hydrolysis, respectively. PilB proteins containing mutant Walker A or Walker B boxes were nonfunctional in vivo and unable to support T4P extension. PilT proteins containing mutant Walker A or Walker B boxes were also nonfunctional in vivo and unable to support T4P retraction. These data provide genetic evidence that both ATP binding and hydrolysis by PilB are essential for T4P extension and that both ATP binding and hydrolysis by PilT are essential for T4P retraction. Thus, PilB and PilT are ATPases that act at distinct steps in the T4P extension/retraction cycle in vivo."],["dc.identifier.doi","10.1128/JB.01793-07"],["dc.identifier.isi","000254323300019"],["dc.identifier.pmid","18223089"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/54630"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Microbiology"],["dc.relation.issn","0021-9193"],["dc.title","PilB and PilT are ATPases acting antagonistically in type IV pilus function in Myxococcus xanthus"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","41"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","FEMS Microbiology Letters"],["dc.bibliographiccitation.lastpage","44"],["dc.bibliographiccitation.volume","182"],["dc.contributor.author","Hofmann, K."],["dc.contributor.author","Heinz, E. B."],["dc.contributor.author","Charles, Trevor C."],["dc.contributor.author","Hoppert, M."],["dc.contributor.author","Liebl, Wolfgang"],["dc.contributor.author","Streit, Wolfgang R."],["dc.date.accessioned","2018-11-07T11:05:22Z"],["dc.date.available","2018-11-07T11:05:22Z"],["dc.date.issued","2000"],["dc.description.abstract","Sinorhizobium meliloti 1021 responds to external biotin signals from alfalfa plants through the bioS regulatory locus. Immunogold labeling and electron microscopy revealed that the BioS protein is located within the S. meliloti cytoplasm. Under biotin-limiting conditions the S. meliloti cell lumen was filled with polyhydroxybutyrate (PHB) granules suggesting that either PHB synthesis or degradation are influenced by biotin. To test this hypothesis a 3-hydroxybutyrate-dehydrogenase-lacZ (bdhA-lacZ) fusion was mobilized into S. meliloti. P-galactosidase tests revealed an overall 3.6-5.2-fold higher bdhA transcription in the presence of added biotin. Comparison of the bdhA and the bioS promoter regions identified several common motifs. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved."],["dc.identifier.doi","10.1111/j.1574-6968.2000.tb08870.x"],["dc.identifier.isi","000084646600007"],["dc.identifier.pmid","10612728"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52057"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","0378-1097"],["dc.title","Sinorhizobium meliloti strain 1021 bioS and bdhA gene transcriptions are both affected by biotin available in defined medium"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Geochimica et Cosmochimica Acta"],["dc.bibliographiccitation.volume","74"],["dc.contributor.author","Hoppert, Michael"],["dc.contributor.author","Dreier, Anne"],["dc.contributor.author","Heller, C."],["dc.contributor.author","Kokoschka, Sebastian"],["dc.contributor.author","Krukenberg, Viola"],["dc.contributor.author","Reitner, Joachim"],["dc.contributor.author","Taviani, Marco"],["dc.contributor.author","Wrede, Christoph"],["dc.date.accessioned","2018-11-07T08:42:40Z"],["dc.date.available","2018-11-07T08:42:40Z"],["dc.date.issued","2010"],["dc.identifier.isi","000283941401208"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/19753"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Pergamon-elsevier Science Ltd"],["dc.publisher.place","Oxford"],["dc.relation.conference","Conference on Goldschmidt 2010 - Earth, Energy, and the Environment"],["dc.relation.eventlocation","Knoxville, TN"],["dc.title","Microbial activity in terrestrial mud volcanoes from the Northern Apennines"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","13"],["dc.bibliographiccitation.journal","Geochimica et Cosmochimica Acta"],["dc.bibliographiccitation.volume","73"],["dc.contributor.author","Heller, C."],["dc.contributor.author","Blumenberg, Martin"],["dc.contributor.author","Dreier, Anne"],["dc.contributor.author","Wrede, Christoph"],["dc.contributor.author","Zilla, Thomas"],["dc.contributor.author","Kokoschka, Sebastian"],["dc.contributor.author","Heim, C."],["dc.contributor.author","Hoppert, Michael"],["dc.contributor.author","Taviani, M."],["dc.contributor.author","Reitner, Joachim"],["dc.date.accessioned","2018-11-07T08:29:13Z"],["dc.date.available","2018-11-07T08:29:13Z"],["dc.date.issued","2009"],["dc.identifier.isi","000267229901266"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/16598"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Pergamon-elsevier Science Ltd"],["dc.publisher.place","Oxford"],["dc.relation.conference","19th Annual VM Goldschmidt Conference"],["dc.relation.eventlocation","Davos, SWITZERLAND"],["dc.title","First results of geo- and biochemical analyses of terrestrial methane-emittingen mud volcanoes in Italy"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","793"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Paläontologische Zeitschrift"],["dc.bibliographiccitation.lastpage","807"],["dc.bibliographiccitation.volume","95"],["dc.contributor.author","Somoza, Luis"],["dc.contributor.author","Rueda, José Luis"],["dc.contributor.author","González, Francisco J."],["dc.contributor.author","Rincón-Tomás, Blanca"],["dc.contributor.author","Medialdea, Teresa"],["dc.contributor.author","Sánchez-Guillamón, Olga"],["dc.contributor.author","Hoppert, Michael"],["dc.contributor.author","Vázquez, Juan T."],["dc.contributor.author","Madureira, Pedro"],["dc.contributor.author","Santofimia, Esther"],["dc.contributor.author","Reitner, Joachim"],["dc.date.accessioned","2022-01-11T14:05:30Z"],["dc.date.available","2022-01-11T14:05:30Z"],["dc.date.issued","2021"],["dc.description.abstract","Abstract Extensive beds of the deep-sea mussel Bathymodiolus mauritanicus (currently also known as Gigantidas mauritanicus ) linked to active cold seeps related to fissure-like activity on Al Gacel mud volcano, Gulf of Cádiz, were filmed and sampled for the first time during the oceanographic expedition SUBVENT-2 aboard R/V Sarmiento de Gamboa. Al Gacel mud volcano is one of up to 80 fluid venting submarine structures (mud volcanoes and mud volcano/diapir complexes) identified in the Gulf of Cádiz as result of explosive venting of hydrocarbon-enriched fluids sourced from deep seated reservoirs. This mud volcano is a cone-shaped edifice, 107 m high, 944 m in diameter constituted by mud breccias and, partially covered by pavements of seep carbonates. Extensive beds of this deep-sea mussel were detected at the northern flank at 810–815 m water depth associated with bacterial mats around intermittent buoyant vertical bubble methane plumes. High methane concentrations were measured in the water column above living mussel beds. Other chemosymbiotic species ( Siboglinum sp., Solemya elarraichensis , Isorropodon sp., Thyasira vulcolutre and Lucinoma asapheus ) were also found in different parts of Al Gacel mud volcano. Al Gacel mud volcano may currently represent one of the most active mud volcanoes in the Gulf of Cádiz, delivering significant amounts of thermogenic hydrocarbon fluids which contribute to foster the extensive chemosynthesis-based communities detected. This finding is of paramount importance for linking extremophile bivalve populations along the North Atlantic, including cold seeps of the Gulf of México, hydrothermal vents of the Mid-Atlantic Ridge and now, detailed documented at the Gulf of Cádiz."],["dc.description.abstract","Abstract Extensive beds of the deep-sea mussel Bathymodiolus mauritanicus (currently also known as Gigantidas mauritanicus ) linked to active cold seeps related to fissure-like activity on Al Gacel mud volcano, Gulf of Cádiz, were filmed and sampled for the first time during the oceanographic expedition SUBVENT-2 aboard R/V Sarmiento de Gamboa. Al Gacel mud volcano is one of up to 80 fluid venting submarine structures (mud volcanoes and mud volcano/diapir complexes) identified in the Gulf of Cádiz as result of explosive venting of hydrocarbon-enriched fluids sourced from deep seated reservoirs. This mud volcano is a cone-shaped edifice, 107 m high, 944 m in diameter constituted by mud breccias and, partially covered by pavements of seep carbonates. Extensive beds of this deep-sea mussel were detected at the northern flank at 810–815 m water depth associated with bacterial mats around intermittent buoyant vertical bubble methane plumes. High methane concentrations were measured in the water column above living mussel beds. Other chemosymbiotic species ( Siboglinum sp., Solemya elarraichensis , Isorropodon sp., Thyasira vulcolutre and Lucinoma asapheus ) were also found in different parts of Al Gacel mud volcano. Al Gacel mud volcano may currently represent one of the most active mud volcanoes in the Gulf of Cádiz, delivering significant amounts of thermogenic hydrocarbon fluids which contribute to foster the extensive chemosynthesis-based communities detected. This finding is of paramount importance for linking extremophile bivalve populations along the North Atlantic, including cold seeps of the Gulf of México, hydrothermal vents of the Mid-Atlantic Ridge and now, detailed documented at the Gulf of Cádiz."],["dc.identifier.doi","10.1007/s12542-021-00594-3"],["dc.identifier.pii","594"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/97676"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-507"],["dc.relation.eissn","1867-6812"],["dc.relation.issn","0031-0220"],["dc.title","A relict oasis of living deep-sea mussels Bathymodiolus and microbial-mediated seep carbonates at newly-discovered active cold seeps in the Gulf of Cádiz, NE Atlantic Ocean"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","624"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Viruses"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Schilling, Tobias"],["dc.contributor.author","Hoppert, Michael"],["dc.contributor.author","Hertel, Robert"],["dc.date.accessioned","2019-07-09T11:49:42Z"],["dc.date.available","2019-07-09T11:49:42Z"],["dc.date.issued","2018"],["dc.description.abstract","We present the recently isolated virus vB_BthP-Goe4 infecting Bacillus thuringiensis HD1. Morphological investigation via transmission electron microscopy revealed key characteristics of the genus Phi29virus, but with an elongated head resulting in larger virion particles of approximately 50 nm width and 120 nm height. Genome sequencing and analysis resulted in a linear phage chromosome of approximately 26 kb, harbouring 40 protein-encoding genes and a packaging RNA. Sequence comparison confirmed the relation to the Phi29virus genus and genomes of other related strains. A global average nucleotide identity analysis of all identified φ29-like viruses revealed the formation of several new groups previously not observed. The largest group includes Goe4 and may significantly expand the genus Phi29virus (Salasvirus) or the Picovirinae subfamily."],["dc.description.sponsorship","Volkswagen Foundation"],["dc.identifier.doi","10.3390/v10110624"],["dc.identifier.pmid","30428528"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15746"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59610"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","MDPI"],["dc.relation.eissn","1999-4915"],["dc.relation.issn","1999-4915"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","570"],["dc.title","Genomic Analysis of the Recent Viral Isolate vB_BthP-Goe4 Reveals Increased Diversity of φ29-Like Phages."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Molecular Neuroscience"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Popova, Blagovesta"],["dc.contributor.author","Wang, Dan"],["dc.contributor.author","Rajavel, Abirami"],["dc.contributor.author","Dhamotharan, Karthikeyan"],["dc.contributor.author","Lázaro, Diana F."],["dc.contributor.author","Gerke, Jennifer"],["dc.contributor.author","Uhrig, Joachim F."],["dc.contributor.author","Hoppert, Michael"],["dc.contributor.author","Outeiro, Tiago Fleming"],["dc.contributor.author","Braus, Gerhard H."],["dc.date.accessioned","2021-06-01T09:42:26Z"],["dc.date.available","2021-06-01T09:42:26Z"],["dc.date.issued","2021"],["dc.description.abstract","Aggregation of α-synuclein (αSyn) into proteinaceous deposits is a pathological hallmark of a range of neurodegenerative diseases including Parkinson’s disease (PD). Numerous lines of evidence indicate that the accumulation of toxic oligomeric and prefibrillar αSyn species may underpin the cellular toxicity and spread of pathology between cells. Therefore, aggregation of αSyn is considered a priority target for drug development, as aggregation inhibitors are expected to reduce αSyn toxicity and serve as therapeutic agents. Here, we used the budding yeast S. cerevisiae as a platform for the identification of short peptides that inhibit αSyn aggregation and toxicity. A library consisting of approximately one million peptide variants was utilized in two high-throughput screening approaches for isolation of library representatives that reduce αSyn-associated toxicity and aggregation. Seven peptides were isolated that were able to suppress specifically αSyn toxicity and aggregation in living cells. Expression of the peptides in yeast reduced the accumulation of αSyn-induced reactive oxygen species and increased cell viability. Next, the peptides were chemically synthesized and probed for their ability to modulate αSyn aggregation in vitro . Two synthetic peptides, K84s and K102s, of 25 and 19 amino acids, respectively, significantly inhibited αSyn oligomerization and aggregation at sub-stoichiometric molar ratios. Importantly, K84s reduced αSyn aggregation in human cells. These peptides represent promising αSyn aggregation antagonists for the development of future therapeutic interventions."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.3389/fnmol.2021.659926"],["dc.identifier.pmid","33912013"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17845"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85254"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/420"],["dc.notes.intern","DOI-Import GROB-425"],["dc.notes.intern","Merged from goescholar"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","1662-5099"],["dc.relation.workinggroup","RG Outeiro (Experimental Neurodegeneration)"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Identification of Two Novel Peptides That Inhibit α-Synuclein Toxicity and Aggregation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","147"],["dc.bibliographiccitation.journal","Nova Hedwigia"],["dc.bibliographiccitation.lastpage","163"],["dc.contributor.author","Heinken, Thilo"],["dc.contributor.author","Rohner, Maria-Sofie"],["dc.contributor.author","Hoppert, Michael"],["dc.date.accessioned","2018-11-07T11:07:15Z"],["dc.date.available","2018-11-07T11:07:15Z"],["dc.date.issued","2007"],["dc.description.abstract","Gametophyte and thallus fragments, respectively, may be an important or even the only mode of reproduction for many bryophytes and lichens species. Until now especially birds and mammals have been identified as potential animal dispersal vectors of fragments. This study investigates the dispersal of bryophyte and lichen fragments by red wood ants which build large nest mounds from plant material and are abundant in European coniferous forests. We sampled nest material from 25 nest mounds in five different pine and spruce forest types in Germany and found numerous fragments of 20 bryophyte and ten lichen species. As they occurred on almost all studied mounds and often in large numbers we conclude that collecting cryptogam fragments as nest material is a characteristic feature for the Formica rufa group in coniferous forests. Species number and composition of fragments on mounds coincided with the epigeic vegetation around ant nests to a large extent: Almost all collected species were present in the vegetation, and dominant fragment species occurred in large amounts in the vicinity of ant nests. Lichen fragments were larger than bryophyte fragments. Certain life forms (weft-forming bryophytes, reindeer lichens) were accumulated on mounds, while others (tall turfs, cup-type Cladonia species) were discriminated, reflecting fragmentation features of species. Collected fragments may regenerate to mature plants if nest mounds are abandoned, and especially if they are lost during transport over several metres. We conclude that dispersal of fragments by red wood ants contributes to maintain epigeic bryophyte and lichen diversity of coniferous forests by supporting colonisation after disturbances, which occur on different spatial and temporal scales."],["dc.identifier.isi","000248754500013"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52513"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Gebruder Borntraeger"],["dc.relation.issn","0029-5035"],["dc.title","Red wood ants (Formica rufa group) disperse bryophyte and lichen fragments on a local scale"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1394"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Applied and Environmental Microbiology"],["dc.bibliographiccitation.lastpage","1401"],["dc.bibliographiccitation.volume","72"],["dc.contributor.author","Gohl, O."],["dc.contributor.author","Friedrich, A."],["dc.contributor.author","Hoppert, M."],["dc.contributor.author","Averhoff, B."],["dc.date.accessioned","2018-11-07T10:20:20Z"],["dc.date.available","2018-11-07T10:20:20Z"],["dc.date.issued","2006"],["dc.description.abstract","Two structurally different appendages, thin and thick pili, are found in members of the genus Acinetobacter. The presence of pilus structures correlates with different phenotypes, such as adherence to surfaces, a trait not only observed in pathogenic Acinetobacter species, as well as motility. However, their distinct individual roles were unknown. To characterize the role of different pili in the physiology of Acinetobacter, we isolated the thin pili from the cell surface of Acinetobacter sp. strain BD413 (recently recognized as representative of Acinetobacter baylyi), a soil bacterium that rapidly takes up naked DNA from its environment. Electron microcopy revealed that the pilus has an external diameter of 2 to 3 nm for single filaments. The filaments are packed into right-handed bundles. The major protein constituting the pilus was purified, and the encoding gene, acuA, was cloned. AcuA was found to be weakly related to the structural subunit of F17 pilli of Escherichia coli. Analyses of the acuA flanking DNA region led to the identification of three closely associated genes, acuD, acuC, and acuG, whose deduced proteins are similar to chaperone, usher, and adhesin of F17-related pill, respectively. ;Transcriptional analyses revealed that acuA expression is maximal in the late-stationary-growth phase. Mutation of acuA led to a loss of thin pili and concomitantly loss of adhesion to polystyrene and erythrocytes but not loss of competence. Therefore, thin pili of Acinetobacter sp. strain BD413 are suggested to be assembled by the chaperone/usher pathway and are involved in adherence to biotic and abiotic surfaces."],["dc.identifier.doi","10.1128/AEM.72.2.1394-1401.2006"],["dc.identifier.isi","000235353100055"],["dc.identifier.pmid","16461692"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/41869"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Microbiology"],["dc.relation.issn","0099-2240"],["dc.title","The thin pili of Acinetobacter sp strain BD413 mediate adhesion to biotic and abiotic surfaces"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]