Kludt, Eugen

[["dc.bibliographiccitation.firstpage","E3"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Chemical Senses"],["dc.bibliographiccitation.lastpage","E4"],["dc.bibliographiccitation.volume","36"],["dc.contributor.author","Junek, Stephan"],["dc.contributor.author","Kludt, Eugen"],["dc.contributor.author","Wolf, Fred"],["dc.contributor.author","Schild, Detlev"],["dc.date.accessioned","2018-11-07T09:00:59Z"],["dc.date.available","2018-11-07T09:00:59Z"],["dc.date.issued","2011"],["dc.identifier.isi","000285414900012"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24299"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.publisher.place","Oxford"],["dc.relation.eventlocation","Avignon, FRANCE"],["dc.relation.issn","0379-864X"],["dc.title","Fast simultaneous imaging of mitral cell populations reveals odor specificity of latency patterns"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","7892"],["dc.bibliographiccitation.issue","20"],["dc.bibliographiccitation.journal","Journal of Neuroscience"],["dc.bibliographiccitation.lastpage","7963"],["dc.bibliographiccitation.volume","35"],["dc.contributor.author","Kludt, Eugen"],["dc.contributor.author","Okom, Camille"],["dc.contributor.author","Brinkmann, Alexander"],["dc.contributor.author","Schild, Detlev"],["dc.date.accessioned","2018-11-07T09:57:09Z"],["dc.date.available","2018-11-07T09:57:09Z"],["dc.date.issued","2015"],["dc.description.abstract","Temperature perception has long been classified as a somesthetic function solely. However, in recent years several studies brought evidence that temperature perception also takes place in the olfactory system of rodents. Temperature has been described as an effective stimulus for sensory neurons of the Grueneberg ganglion located at the entrance of the nose. Here, we investigate whether a neuronal trace of temperature stimulation can be observed in the glomeruli and mitral cells of the olfactory bulb, using calcium imaging and fast line-scanning microscopy. We show in the Xenopus tadpole system that the gamma-glomerulus, which receives input from olfactory neurons, is highly sensitive to temperature drops at the olfactory epithelium. We observed that thermo-induced activity in the gamma-glomerulus is conveyed to the mitral cells innervating this specific neuropil. Surprisingly, a substantial number of thermosensitive mitral cells were also chemosensitive. Moreover, we report another unique feature of the gamma-glomerulus: it receives ipsilateral and contralateral afferents. The latter fibers pass through the contralateral bulb, cross the anterior commissure, and then run to the ipsilateral olfactory bulb, where they target the gamma-glomerulus. Temperature drops at the contralateral olfactory epithelium also induced responses in the gamma-glomerulus and in mitral cells. Temperature thus appears to be a relevant physiological input to the Xenopus olfactory system. Each olfactory bulb integrates and codes temperature signals originating from receptor neurons of the ipsilateral and contralateral nasal cavities. Finally, temperature and chemical information is processed in shared cellular networks."],["dc.identifier.doi","10.1523/JNEUROSCI.0571-15.2015"],["dc.identifier.isi","000356670000019"],["dc.identifier.pmid","25995474"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/37098"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Soc Neuroscience"],["dc.relation.issn","0270-6474"],["dc.title","Integrating Temperature with Odor Processing in the Olfactory Bulb"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","28041"],["dc.bibliographiccitation.issue","32"],["dc.bibliographiccitation.journal","Journal of Biological Chemistry"],["dc.bibliographiccitation.lastpage","28048"],["dc.bibliographiccitation.volume","286"],["dc.contributor.author","Breunig, Esther"],["dc.contributor.author","Kludt, Eugen"],["dc.contributor.author","Czesnik, Dirk"],["dc.contributor.author","Schild, Detlev"],["dc.date.accessioned","2018-11-07T08:53:16Z"],["dc.date.available","2018-11-07T08:53:16Z"],["dc.date.issued","2011"],["dc.description.abstract","Many olfactory receptor neurons use a cAMP-dependent transduction mechanism to transduce odorants into depolarizations. This signaling cascade is characterized by a sequence of two currents: a cation current through cyclic nucleotide-gated channels followed by a chloride current through calcium-activated chloride channels. To date, it is not possible to interfere with these generator channels under physiological conditions with potent and specific blockers. In this study we identified the styryl dye FM1-43 as a potent blocker of native olfactory cyclic nucleotide-gated channels. Furthermore, we characterized this substance to stain olfactory receptor neurons that are endowed with cAMP-dependent transduction. This allows optical differentiation and pharmacological interference with olfactory receptor neurons at the level of the signal transduction."],["dc.identifier.doi","10.1074/jbc.M111.233890"],["dc.identifier.isi","000293557800022"],["dc.identifier.pmid","21646359"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7624"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22365"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Biochemistry Molecular Biology Inc"],["dc.relation.issn","0021-9258"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","The Styryl Dye FM1-43 Suppresses Odorant Responses in a Subset of Olfactory Neurons by Blocking Cyclic Nucleotide-gated (CNG) Channels"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1965"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Cellular and Molecular Life Sciences"],["dc.bibliographiccitation.lastpage","1984"],["dc.bibliographiccitation.volume","70"],["dc.contributor.author","Gliem, Sebastian"],["dc.contributor.author","Syed, Adnan S."],["dc.contributor.author","Sansone, Alfredo"],["dc.contributor.author","Kludt, Eugen"],["dc.contributor.author","Tantalaki, Evangelia"],["dc.contributor.author","Hassenkloever, Thomas"],["dc.contributor.author","Korsching, Sigrun I."],["dc.contributor.author","Manzini, Ivan"],["dc.date.accessioned","2018-11-07T09:24:19Z"],["dc.date.available","2018-11-07T09:24:19Z"],["dc.date.issued","2013"],["dc.description.abstract","In contrast to the single sensory surface present in teleost fishes, several spatially segregated subsystems with distinct molecular and functional characteristics define the mammalian olfactory system. However, the evolutionary steps of that transition remain unknown. Here we analyzed the olfactory system of an early diverging tetrapod, the amphibian Xenopus laevis, and report for the first time the existence of two odor-processing streams, sharply segregated in the main olfactory bulb and partially segregated in the olfactory epithelium of pre-metamorphic larvae. A lateral odor-processing stream is formed by microvillous receptor neurons and is characterized by amino acid responses and G alpha(o)/G alpha(i) as probable signal transducers, whereas a medial stream formed by ciliated receptor neurons is characterized by responses to alcohols, aldehydes, and ketones, and G alpha(olf)/cAMP as probable signal transducers. To reveal candidates for the olfactory receptors underlying these two streams, the spatial distribution of 12 genes from four olfactory receptor gene families was determined. Several class II and some class I odorant receptors (ORs) mimic the spatial distribution observed for the medial stream, whereas a trace amine-associated receptor closely parallels the spatial pattern of the lateral odor-processing stream. Other olfactory receptors (some class I odorant receptors and vomeronasal type 1 receptors) and odor responses (to bile acids, amines) were not lateralized, the latter not even in the olfactory bulb, suggesting an incomplete segregation. Thus, the olfactory system of X. laevis exhibits an intermediate stage of segregation and as such appears well suited to investigate the molecular driving forces behind olfactory regionalization."],["dc.identifier.doi","10.1007/s00018-012-1226-8"],["dc.identifier.isi","000319220100007"],["dc.identifier.pmid","23269434"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10273"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29797"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","Basel"],["dc.relation.issn","1420-682X"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Bimodal processing of olfactory information in an amphibian nose: odor responses segregate into a medial and a lateral stream"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Chemical Senses"],["dc.bibliographiccitation.volume","36"],["dc.contributor.author","Gliem, Sebastian"],["dc.contributor.author","Kludt, Eugen"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Manzini, Ivan"],["dc.date.accessioned","2018-11-07T09:00:58Z"],["dc.date.available","2018-11-07T09:00:58Z"],["dc.date.issued","2011"],["dc.format.extent","E71"],["dc.identifier.isi","000285414900190"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24298"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.publisher.place","Oxford"],["dc.relation.conference","20th Congress of European Chemoreception Research Organization (ECRO-2010)"],["dc.relation.eventlocation","Avignon, FRANCE"],["dc.relation.issn","0379-864X"],["dc.title","Subsystem-specific odorant-processing in the main olfactory system of larval Xenopus laevis"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","872"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Neuron"],["dc.bibliographiccitation.lastpage","884"],["dc.bibliographiccitation.volume","67"],["dc.contributor.author","Junek, Stephan"],["dc.contributor.author","Kludt, Eugen"],["dc.contributor.author","Wolf, Fred"],["dc.contributor.author","Schild, Detlev"],["dc.date.accessioned","2017-09-07T11:45:41Z"],["dc.date.available","2017-09-07T11:45:41Z"],["dc.date.issued","2010"],["dc.description.abstract","The encoding of odors by spatiotemporal patterns of mitral/tufted (M/T) cells in the vertebrate olfactory bulb has been discussed controversially. Motivated by temporal constraints from behavioral studies, we investigated the information contained in odor-evoked first-spike latencies. Using simultaneous recordings of dozens of M/T cells with a high temporal resolution and quantitative ensemble correlation techniques, we show that latency patterns, and in particular latency rank patterns, are highly odor specific and reproducible. They reliably predict the odor identity as well as the odor concentration on a single-trial basis and on short timescales—in fact, more reliably than patterns of firing rates. Furthermore, we show that latency ranks exhibit a better reproducibility at the level of M/T cells than in olfactory receptor neurons. Our results suggest that the latency patterns of M/T cells contain all the information higher brain centers need to identify odors and their concentrations."],["dc.identifier.doi","10.1016/j.neuron.2010.08.005"],["dc.identifier.gro","3151845"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6314"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/8672"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","chake"],["dc.relation.issn","0896-6273"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Olfactory Coding with Patterns of Response Latencies"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e54108"],["dc.bibliographiccitation.issue","112"],["dc.bibliographiccitation.journal","Journal of Visualized Experiments"],["dc.contributor.author","Brinkmann, Alexander"],["dc.contributor.author","Okom, Camille"],["dc.contributor.author","Kludt, Eugen"],["dc.contributor.author","Schild, Detlev"],["dc.date.accessioned","2018-11-07T10:13:01Z"],["dc.date.available","2018-11-07T10:13:01Z"],["dc.date.issued","2016"],["dc.description.abstract","The olfactory system, specialized in the detection, integration and processing of chemical molecules is likely the most thoroughly studied sensory system. However, there is piling evidence that olfaction is not solely limited to chemical sensitivity, but also includes temperature sensitivity. Premetamorphic Xenopus laevis are translucent animals, with protruding nasal cavities deprived of the cribriform plate separating the nose and the olfactory bulb. These characteristics make them well suited for studying olfaction, and particularly thermosensitivity. The present article describes the complete procedure for measuring temperature responses in the olfactory bulb of X. laevis larvae. Firstly, the electroporation of olfactory receptor neurons (ORNs) is performed with spectrally distinct dyes loaded into the nasal cavities in order to stain their axon terminals in the bulbar neuropil. The differential staining between left and right receptor neurons serves to identify the gamma-glomerulus as the only structure innervated by contralateral presynaptic afferents. Secondly, the electroporation is combined with focal bolus loading in the olfactory bulb in order to stain mitral cells and their dendrites. The 3D brain volume is then scanned under line-illumination microscopy for the acquisition of fast calcium imaging data while small temperature drops are induced at the olfactory epithelium. Lastly, the post-acquisition analysis allows the morphological reconstruction of the thermosensitive network comprising the gamma-glomerulus and its innervating mitral cells, based on specific temperature-induced Ca2+ traces. Using chemical odorants as stimuli in addition to temperature jumps enables the comparison between thermosensitive and chemosensitive networks in the olfactory bulb."],["dc.identifier.doi","10.3791/54108"],["dc.identifier.isi","000380264100065"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/40353"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Journal Of Visualized Experiments"],["dc.relation.issn","1940-087X"],["dc.title","Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of Xenopus laevis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]