Hahn, Andreas

[["dc.bibliographiccitation.firstpage","656"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Pathogens"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Tanida, Konstantin"],["dc.contributor.author","Hahn, Andreas"],["dc.contributor.author","Eberhardt, Kirsten Alexandra"],["dc.contributor.author","Tannich, Egbert"],["dc.contributor.author","Landt, Olfert"],["dc.contributor.author","Kann, Simone"],["dc.contributor.author","Feldt, Torsten"],["dc.contributor.author","Sarfo, Fred Stephen"],["dc.contributor.author","Di Cristanziano, Veronica"],["dc.contributor.author","Loderstädt, Ulrike"],["dc.contributor.author","Frickmann, Hagen"],["dc.date.accessioned","2021-08-12T07:46:03Z"],["dc.date.available","2021-08-12T07:46:03Z"],["dc.date.issued","2021"],["dc.description.abstract","Microsporidiosis is an infection predominantly occurring in immunosuppressed patients and infrequently also in travelers. This study was performed to comparatively evaluate the diagnostic accuracy of real-time PCR assays targeting microsporidia with etiological relevance in the stool of human patients in a latent class analysis-based test comparison without a reference standard with perfect accuracy. Thereby, two one-tube real-time PCR assays and two two-tube real-time PCR assays targeting Enterocytozoon bieneusi and Encephalocytozoon spp. were included in the assessment with reference stool material (20), stool samples from Ghanaian HIV-positive patients (903), and from travelers, migrants and Colombian indigenous people (416). Sensitivity of the assays ranged from 60.4% to 97.4% and specificity from 99.1% to 100% with substantial agreement according to Cohen\\’s kappa of 79.6%. Microsporidia DNA was detected in the reference material and the stool of the HIV patients but not in the stool of the travelers, migrants, and the Colombian indigenous people. Accuracy-adjusted prevalence was 5.8% (n = 78) for the study population as a whole. In conclusion, reliable detection of enteric disease-associated microsporidia in stool samples by real-time PCR could be demonstrated, but sensitivity between the compared microsporidia-specific real-time PCR assays varied."],["dc.description.abstract","Microsporidiosis is an infection predominantly occurring in immunosuppressed patients and infrequently also in travelers. This study was performed to comparatively evaluate the diagnostic accuracy of real-time PCR assays targeting microsporidia with etiological relevance in the stool of human patients in a latent class analysis-based test comparison without a reference standard with perfect accuracy. Thereby, two one-tube real-time PCR assays and two two-tube real-time PCR assays targeting Enterocytozoon bieneusi and Encephalocytozoon spp. were included in the assessment with reference stool material (20), stool samples from Ghanaian HIV-positive patients (903), and from travelers, migrants and Colombian indigenous people (416). Sensitivity of the assays ranged from 60.4% to 97.4% and specificity from 99.1% to 100% with substantial agreement according to Cohen’s kappa of 79.6%. Microsporidia DNA was detected in the reference material and the stool of the HIV patients but not in the stool of the travelers, migrants, and the Colombian indigenous people. Accuracy-adjusted prevalence was 5.8% (n = 78) for the study population as a whole. In conclusion, reliable detection of enteric disease-associated microsporidia in stool samples by real-time PCR could be demonstrated, but sensitivity between the compared microsporidia-specific real-time PCR assays varied."],["dc.description.sponsorship","Bundesministerium der Verteidigung"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.3390/pathogens10060656"],["dc.identifier.pii","pathogens10060656"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/88607"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-448"],["dc.relation.eissn","2076-0817"],["dc.relation.haserratum","/handle/2/105036"],["dc.relation.orgunit","Institut für Krankenhaushygiene und Infektiologie"],["dc.rights","CC BY 4.0"],["dc.title","Comparative Assessment of In-House Real-Time PCRs Targeting Enteric Disease-Associated Microsporidia in Human Stool Samples"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","S1477893921000831"],["dc.bibliographiccitation.firstpage","102042"],["dc.bibliographiccitation.journal","Travel Medicine and Infectious Disease"],["dc.bibliographiccitation.volume","41"],["dc.contributor.author","Frickmann, Hagen"],["dc.contributor.author","Hoffmann, Tanja"],["dc.contributor.author","Köller, Thomas"],["dc.contributor.author","Hahn, Andreas"],["dc.contributor.author","Podbielski, Andreas"],["dc.contributor.author","Landt, Olfert"],["dc.contributor.author","Loderstädt, Ulrike"],["dc.contributor.author","Tannich, Egbert"],["dc.date.accessioned","2021-09-01T06:42:29Z"],["dc.date.available","2021-09-01T06:42:29Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1016/j.tmaid.2021.102042"],["dc.identifier.pii","S1477893921000831"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/89065"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-455"],["dc.relation.issn","1477-8939"],["dc.title","Comparison of five commercial real-time PCRs for in-vitro diagnosis of Entamoeba histolytica, Giardia duodenalis, Cryptosporidium spp., Cyclospora cayetanensis, and Dientamoeba fragilis in human stool samples"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","78"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Diagnostics"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Dörschug, Anja"],["dc.contributor.author","Schwanbeck, Julian"],["dc.contributor.author","Hahn, Andreas"],["dc.contributor.author","Hillebrecht, Anke"],["dc.contributor.author","Blaschke, Sabine"],["dc.contributor.author","Mese, Kemal"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Dierks, Sascha"],["dc.contributor.author","Frickmann, Hagen"],["dc.contributor.author","Zautner, Andreas E."],["dc.date.accessioned","2021-04-14T08:29:44Z"],["dc.date.available","2021-04-14T08:29:44Z"],["dc.date.issued","2021"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.3390/diagnostics11010078"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82978"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","2075-4418"],["dc.relation.orgunit","Institut für Medizinische Mikrobiologie"],["dc.rights","CC BY 4.0"],["dc.title","Comparison of Five Serological Assays for the Detection of SARS-CoV-2 Antibodies"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","518"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Der Nervenarzt"],["dc.bibliographiccitation.lastpage","529"],["dc.bibliographiccitation.volume","91"],["dc.contributor.author","Ziegler, Andreas"],["dc.contributor.author","Wilichowski, Ekkehard"],["dc.contributor.author","Schara, Ulrike"],["dc.contributor.author","Hahn, Andreas"],["dc.contributor.author","Müller-Felber, Wolfgang"],["dc.contributor.author","Johannsen, Jessika"],["dc.contributor.author","von der Hagen, Maja"],["dc.contributor.author","von Moers, Arpad"],["dc.contributor.author","Stoltenburg, Corinna"],["dc.contributor.author","Saffari, Afshin"],["dc.contributor.author","Walter, Maggie C."],["dc.contributor.author","Husain, Ralf A."],["dc.contributor.author","Pechmann, Astrid"],["dc.contributor.author","Köhler, Cornelia"],["dc.contributor.author","Horber, Veronka"],["dc.contributor.author","Schwartz, Oliver"],["dc.contributor.author","Kirschner, Janbernd"],["dc.date.accessioned","2020-12-10T14:08:39Z"],["dc.date.available","2020-12-10T14:08:39Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1007/s00115-020-00919-8"],["dc.identifier.eissn","1433-0407"],["dc.identifier.issn","0028-2804"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/70509"],["dc.language.iso","de"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Handlungsempfehlungen zur Gentherapie der spinalen Muskelatrophie mit Onasemnogene Abeparvovec – AVXS-101"],["dc.title.alternative","Recommendations for gene therapy of spinal muscular atrophy with onasemnogene abeparvovec—AVXS-101. Consensus paper of the German representatives of the Society for Pediatric Neurology (GNP) and the German treatment centers with collaboration of the medical scientific advisory board of the German Society for Muscular Diseases (DGM)"],["dc.title.subtitle","Konsensuspapier der deutschen Vertretung der Gesellschaft für Neuropädiatrie (GNP) und der deutschen Behandlungszentren unter Mitwirkung des Medizinisch-Wissenschaftlichen Beirates der Deutschen Gesellschaft für Muskelkranke (DGM) e. V."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","929"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Antibiotics"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Hahn, Andreas"],["dc.contributor.author","Frickmann, Hagen"],["dc.contributor.author","Loderstädt, Ulrike"],["dc.date.accessioned","2021-10-01T09:58:21Z"],["dc.date.available","2021-10-01T09:58:21Z"],["dc.date.issued","2021"],["dc.description.abstract","Prescribed antibiotic treatments which do not match the therapeutic requirements of potentially co-existing undetected sexually transmitted infections (STIs) can facilitate the selection of antibiotic-drug-resistant clones. To reduce this risk, this modelling assessed the potential applicability of reliable rapid molecular test assays targeting bacterial STI prior to the prescription of antibiotic drugs. The modelling was based on the prevalence of three bacterial STIs in German heterosexual and men-having-sex-with-men (MSM) populations, as well as on reported test characteristics of respective assays. In the case of the application of rapid molecular STI assays for screening, the numbers needed to test in order to correctly identify any of the included bacterial STIs ranged from 103 to 104 for the heterosexual population and from 5 to 14 for the MSM population. The number needed to harm—defined as getting a false negative result for any of the STIs and a false positive signal for another one, potentially leading to an even more inappropriate adaptation of antibiotic therapy than without any STI screening—was at least 208,995 for the heterosexuals and 16,977 for the MSM. Therefore, the screening approach may indeed be suitable to avoid unnecessary selective pressure on bacterial causes of sexually transmitted infections."],["dc.description.abstract","Prescribed antibiotic treatments which do not match the therapeutic requirements of potentially co-existing undetected sexually transmitted infections (STIs) can facilitate the selection of antibiotic-drug-resistant clones. To reduce this risk, this modelling assessed the potential applicability of reliable rapid molecular test assays targeting bacterial STI prior to the prescription of antibiotic drugs. The modelling was based on the prevalence of three bacterial STIs in German heterosexual and men-having-sex-with-men (MSM) populations, as well as on reported test characteristics of respective assays. In the case of the application of rapid molecular STI assays for screening, the numbers needed to test in order to correctly identify any of the included bacterial STIs ranged from 103 to 104 for the heterosexual population and from 5 to 14 for the MSM population. The number needed to harm—defined as getting a false negative result for any of the STIs and a false positive signal for another one, potentially leading to an even more inappropriate adaptation of antibiotic therapy than without any STI screening—was at least 208,995 for the heterosexuals and 16,977 for the MSM. Therefore, the screening approach may indeed be suitable to avoid unnecessary selective pressure on bacterial causes of sexually transmitted infections."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.3390/antibiotics10080929"],["dc.identifier.pii","antibiotics10080929"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/90046"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-469"],["dc.relation.eissn","2079-6382"],["dc.rights","CC BY 4.0"],["dc.title","Testing as Prevention of Resistance in Bacteria Causing Sexually Transmitted Infections—A Population-Based Model for Germany"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","188"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Pathogens"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Hoffmann, Tanja"],["dc.contributor.author","Hahn, Andreas"],["dc.contributor.author","Verweij, Jaco J."],["dc.contributor.author","Leboulle, Gérard"],["dc.contributor.author","Landt, Olfert"],["dc.contributor.author","Strube, Christina"],["dc.contributor.author","Kann, Simone"],["dc.contributor.author","Dekker, Denise"],["dc.contributor.author","May, Jürgen"],["dc.contributor.author","Frickmann, Hagen"],["dc.contributor.author","Loderstädt, Ulrike"],["dc.date.accessioned","2021-04-14T08:29:37Z"],["dc.date.available","2021-04-14T08:29:37Z"],["dc.date.issued","2021"],["dc.description.sponsorship","Bundesministerium der Verteidigung"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.3390/pathogens10020188"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82947"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","2076-0817"],["dc.relation.orgunit","Institut für Krankenhaushygiene und Infektiologie"],["dc.rights","CC BY 4.0"],["dc.title","Differing Effects of Standard and Harsh Nucleic Acid Extraction Procedures on Diagnostic Helminth Real-Time PCRs Applied to Human Stool Samples"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1131"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Pathogens"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Weinreich, Felix"],["dc.contributor.author","Hahn, Andreas"],["dc.contributor.author","Eberhardt, Kirsten Alexandra"],["dc.contributor.author","Feldt, Torsten"],["dc.contributor.author","Sarfo, Fred Stephen"],["dc.contributor.author","Di Cristanziano, Veronica"],["dc.contributor.author","Frickmann, Hagen"],["dc.contributor.author","Loderstädt, Ulrike"],["dc.date.accessioned","2021-12-01T09:24:07Z"],["dc.date.available","2021-12-01T09:24:07Z"],["dc.date.issued","2021"],["dc.description.abstract","As qualified microscopy of enteric parasitoses as defined by high diagnostic accuracy is difficult to maintain in non-endemic areas due to scarce opportunities for practicing with positive sample materials, molecular diagnostic options provide less investigator-dependent alternatives. Here, we compared three molecular targets for the real-time PCR-based detection of Cryptosporidium spp. From a population of 1000 individuals comprising both Ghanaian HIV (human immunodeficiency virus) patients and military returnees after deployment in the tropics, stool samples were assessed for Cryptosporidium spp. by real-time PCR targeting the small subunit ribosomal RNA (SSU rRNA) gene, the Cryptosporidium oocyst wall (COWP) gene, and the DnaJ-like protein gene (DnaJ), respectively. In declining order, sensitivity of 100% for the SSU rRNA gene PCR, 90.0% for the COWP PCR and 88.8% for the DnaJ PCR, respectively, as well as specificity of 99.6% for the COWP PCR and 96.9% for both the SSU rRNA gene PCR and the DnaJ PCR, respectively, were recorded. Substantial agreement (kappa value 0.663) between the three assays was observed. Further, an accuracy-adjusted Cryptosporidium spp. prevalence of 6.0% was calculated for the study population. In conclusion, none of the assessed real-time PCR assays were associated with perfect test accuracy. However, a combination of highly sensitive SSU rRNA gene PCR for screening purposes and more specific COWP PCR for confirmatory testing should allow reliable diagnosis of Cryptosporidium spp. in stool samples even in low prevalence settings."],["dc.description.abstract","As qualified microscopy of enteric parasitoses as defined by high diagnostic accuracy is difficult to maintain in non-endemic areas due to scarce opportunities for practicing with positive sample materials, molecular diagnostic options provide less investigator-dependent alternatives. Here, we compared three molecular targets for the real-time PCR-based detection of Cryptosporidium spp. From a population of 1000 individuals comprising both Ghanaian HIV (human immunodeficiency virus) patients and military returnees after deployment in the tropics, stool samples were assessed for Cryptosporidium spp. by real-time PCR targeting the small subunit ribosomal RNA (SSU rRNA) gene, the Cryptosporidium oocyst wall (COWP) gene, and the DnaJ-like protein gene (DnaJ), respectively. In declining order, sensitivity of 100% for the SSU rRNA gene PCR, 90.0% for the COWP PCR and 88.8% for the DnaJ PCR, respectively, as well as specificity of 99.6% for the COWP PCR and 96.9% for both the SSU rRNA gene PCR and the DnaJ PCR, respectively, were recorded. Substantial agreement (kappa value 0.663) between the three assays was observed. Further, an accuracy-adjusted Cryptosporidium spp. prevalence of 6.0% was calculated for the study population. In conclusion, none of the assessed real-time PCR assays were associated with perfect test accuracy. However, a combination of highly sensitive SSU rRNA gene PCR for screening purposes and more specific COWP PCR for confirmatory testing should allow reliable diagnosis of Cryptosporidium spp. in stool samples even in low prevalence settings."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.3390/pathogens10091131"],["dc.identifier.pii","pathogens10091131"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/94849"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-478"],["dc.relation.eissn","2076-0817"],["dc.relation.orgunit","Institut für Krankenhaushygiene und Infektiologie"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Comparison of Three Real-Time PCR Assays Targeting the SSU rRNA Gene, the COWP Gene and the DnaJ-Like Protein Gene for the Diagnosis of Cryptosporidium spp. in Stool Samples"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Neuropediatrics"],["dc.contributor.author","Holzwarth, Johanna"],["dc.contributor.author","Minopoli, Nadja"],["dc.contributor.author","Pfrimmer, Charlotte"],["dc.contributor.author","Smitka, Martin"],["dc.contributor.author","Borrel, Sabine"],["dc.contributor.author","Kirschner, Janbernd"],["dc.contributor.author","Muschol, Nicole"],["dc.contributor.author","Hartmann, Hans"],["dc.contributor.author","Hennermann, Julia B."],["dc.contributor.author","Neubauer, Bernd A."],["dc.contributor.author","Hahn, Andreas"],["dc.date.accessioned","2022-01-11T14:05:48Z"],["dc.date.available","2022-01-11T14:05:48Z"],["dc.date.issued","2021"],["dc.description.abstract","Abstract Little is known about clinical symptomatology and genetics of juvenile onset Pompe disease (JOPD). The aims of this study were to analyze how these children are diagnosed, what clinical problems they have, and how phenotype is related to genotype. To accomplish this, we analyzed retrospectively data of 34 patients diagnosed after their first and before completion of their 18th birthday. Median age at diagnosis was 3.9 (range 1.1–17) years. Eight patients (23.5%) developed initial symptoms in the first year, 12 (35%) between 1 and 7 years, and 6 (18%) thereafter. Eight (23.5%) had no clinical symptoms at the time of diagnosis. Indications for diagnostics were a positive family history in three (9%), hyperCKemia in eight (23.5%), motor developmental delay in three (9%), and muscle weakness and/or pain in 17 (50%). Rare clinical signs were failure to thrive, recurrent diarrhea, and suspected hepatopathy with glycogen storage. Thirty-two different mutations were identified. Twenty-seven patients (79.5%) carried the milder c.32–13T > G mutation, known to be associated with a broad range of phenotypes. Three out of eight patients manifesting within the first year of life showed generalized muscle weakness, hypertrophic cardiomyopathy, and had to be ventilated during the course of disease, thereby demonstrating clinical overlap with infantile onset Pompe disease. These findings demonstrate that the phenotype of JOPD is broad and that the differential is not only restricted to neuromuscular disorders. Genotypic analysis was useful to delineate subjects with early onset JOPD from those with IOPD, but overall genotype–phenotype correlation was poor."],["dc.description.abstract","Abstract Little is known about clinical symptomatology and genetics of juvenile onset Pompe disease (JOPD). The aims of this study were to analyze how these children are diagnosed, what clinical problems they have, and how phenotype is related to genotype. To accomplish this, we analyzed retrospectively data of 34 patients diagnosed after their first and before completion of their 18th birthday. Median age at diagnosis was 3.9 (range 1.1–17) years. Eight patients (23.5%) developed initial symptoms in the first year, 12 (35%) between 1 and 7 years, and 6 (18%) thereafter. Eight (23.5%) had no clinical symptoms at the time of diagnosis. Indications for diagnostics were a positive family history in three (9%), hyperCKemia in eight (23.5%), motor developmental delay in three (9%), and muscle weakness and/or pain in 17 (50%). Rare clinical signs were failure to thrive, recurrent diarrhea, and suspected hepatopathy with glycogen storage. Thirty-two different mutations were identified. Twenty-seven patients (79.5%) carried the milder c.32–13T > G mutation, known to be associated with a broad range of phenotypes. Three out of eight patients manifesting within the first year of life showed generalized muscle weakness, hypertrophic cardiomyopathy, and had to be ventilated during the course of disease, thereby demonstrating clinical overlap with infantile onset Pompe disease. These findings demonstrate that the phenotype of JOPD is broad and that the differential is not only restricted to neuromuscular disorders. Genotypic analysis was useful to delineate subjects with early onset JOPD from those with IOPD, but overall genotype–phenotype correlation was poor."],["dc.identifier.doi","10.1055/s-0041-1735250"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/97751"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-507"],["dc.relation.eissn","1439-1899"],["dc.relation.issn","0174-304X"],["dc.title","Clinical and Genetic Aspects of Juvenile Onset Pompe Disease"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","826"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Pathogens"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Tanida, Konstantin"],["dc.contributor.author","Balczun, Carsten"],["dc.contributor.author","Hahn, Andreas"],["dc.contributor.author","Veit, Alexandra"],["dc.contributor.author","Nickel, Beatrice"],["dc.contributor.author","Poppert, Sven"],["dc.contributor.author","Scheid, Patrick Leander"],["dc.contributor.author","Hagen, Ralf Matthias"],["dc.contributor.author","Frickmann, Hagen"],["dc.contributor.author","Tannich, Egbert"],["dc.contributor.author","Loderstädt, Ulrike"],["dc.date.accessioned","2021-08-12T07:46:04Z"],["dc.date.available","2021-08-12T07:46:04Z"],["dc.date.issued","2021"],["dc.description.abstract","To perform PCR from serum for the diagnosis of visceral leishmaniasis is convenient and much less invasive than the examination of deeper compartments such as bone marrow. We compared three Leishmania-specific real-time PCRs with three different molecular targets (kinetoplast DNA, the small subunit-ribosomal RNA-(ssrRNA-)gene, the glucose-6-phosphate isomerase-(gpi-)gene) regarding their sensitivity and specificity in human serum. Residual sera from previous diagnostic assessments at the German National Reference Center for Tropical Pathogens Bernhard Nocht Institute for Tropical Medicine Hamburg and the Swiss Tropical and Public Health Institute were used. The sensitivities of kinetoplast DNA-PCR, ssrRNA-gene PCR, and gpi-PCR were 93.3%, 73.3%, and 33.3%, respectively, with 15 initial serum samples from visceral leishmaniasis patients, as well as 9.1%, 9.1%, and 0.0%, respectively, with 11 follow-up serum samples taken at various time points following anti-leishmanial therapy. Specificity was 100.0% in all assays as recorded with 1.137 serum samples from deployed soldiers and migrants without clinical suspicion of visceral leishmaniasis. Kinetoplast-DNA PCR from serum was confirmed as a sensitive and specific approach for the diagnosis of visceral leishmaniasis. The results also indicate the suitability of serum PCR for diagnostic follow-up after therapy, in particular regarding therapeutic failure in case of persisting positive PCR results."],["dc.description.abstract","To perform PCR from serum for the diagnosis of visceral leishmaniasis is convenient and much less invasive than the examination of deeper compartments such as bone marrow. We compared three Leishmania-specific real-time PCRs with three different molecular targets (kinetoplast DNA, the small subunit-ribosomal RNA-(ssrRNA-)gene, the glucose-6-phosphate isomerase-(gpi-)gene) regarding their sensitivity and specificity in human serum. Residual sera from previous diagnostic assessments at the German National Reference Center for Tropical Pathogens Bernhard Nocht Institute for Tropical Medicine Hamburg and the Swiss Tropical and Public Health Institute were used. The sensitivities of kinetoplast DNA-PCR, ssrRNA-gene PCR, and gpi-PCR were 93.3%, 73.3%, and 33.3%, respectively, with 15 initial serum samples from visceral leishmaniasis patients, as well as 9.1%, 9.1%, and 0.0%, respectively, with 11 follow-up serum samples taken at various time points following anti-leishmanial therapy. Specificity was 100.0% in all assays as recorded with 1.137 serum samples from deployed soldiers and migrants without clinical suspicion of visceral leishmaniasis. Kinetoplast-DNA PCR from serum was confirmed as a sensitive and specific approach for the diagnosis of visceral leishmaniasis. The results also indicate the suitability of serum PCR for diagnostic follow-up after therapy, in particular regarding therapeutic failure in case of persisting positive PCR results."],["dc.description.sponsorship","Bundesministerium der Verteidigung"],["dc.identifier.doi","10.3390/pathogens10070826"],["dc.identifier.pii","pathogens10070826"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/88609"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-448"],["dc.publisher","MDPI"],["dc.relation.eissn","2076-0817"],["dc.rights","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Comparison of Three In-House Real PCR Assays Targeting Kinetoplast DNA, the Small Subunit Ribosomal RNA Gene and the Glucose-6-Phosphate Isomerase Gene for the Detection of Leishmania spp. in Human Serum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","426"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Diagnostics"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Dörschug, Anja"],["dc.contributor.author","Frickmann, Hagen"],["dc.contributor.author","Schwanbeck, Julian"],["dc.contributor.author","Yilmaz, Elif"],["dc.contributor.author","Mese, Kemal"],["dc.contributor.author","Hahn, Andreas"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Zautner, Andreas E."],["dc.date.accessioned","2021-04-14T08:27:55Z"],["dc.date.available","2021-04-14T08:27:55Z"],["dc.date.issued","2021"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.3390/diagnostics11030426"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82450"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","2075-4418"],["dc.relation.orgunit","Institut für Medizinische Mikrobiologie"],["dc.rights","CC BY 4.0"],["dc.title","Comparative Assessment of Sera from Individuals after S-Gene RNA-Based SARS-CoV-2 Vaccination with Spike-Protein-Based and Nucleocapsid-Based Serological Assays"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]