Smyer Yu, Dan

[["dc.bibliographiccitation.firstpage","407"],["dc.bibliographiccitation.lastpage","429"],["dc.contributor.author","van der Veer, Peter"],["dc.contributor.author","Ngo, Tam"],["dc.contributor.author","Smyer Yu, Dan"],["dc.contributor.editor","Appleby, R. Scott"],["dc.contributor.editor","Omer, Atalia"],["dc.contributor.editor","Little, David"],["dc.date.accessioned","2018-02-20T10:56:00Z"],["dc.date.available","2018-02-20T10:56:00Z"],["dc.date.issued","2015"],["dc.identifier.doi","10.1093/oxfordhb/9780199731640.013.0016"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/12373"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.publisher","Oxford University Press"],["dc.publisher.place","Oxford"],["dc.relation.doi","10.1093/oxfordhb/9780199731640.001.0001"],["dc.relation.isbn","978-0-19-973164-0"],["dc.relation.ispartof","Oxford Handbook of Religion, Conflict, and Peacebuilding"],["dc.title","Religion and peace in Asia"],["dc.type","book_chapter"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","479"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Lipids"],["dc.bibliographiccitation.lastpage","494"],["dc.bibliographiccitation.volume","55"],["dc.contributor.author","Vollheyde, Katharina"],["dc.contributor.author","Yu, Dan"],["dc.contributor.author","Hornung, Ellen"],["dc.contributor.author","Herrfurth, Cornelia"],["dc.contributor.author","Feussner, Ivo"],["dc.date.accessioned","2021-04-14T08:26:52Z"],["dc.date.available","2021-04-14T08:26:52Z"],["dc.date.issued","2020"],["dc.description.abstract","Abstract Wax esters (WE) belong to the class of neutral lipids. They are formed by an esterification of a fatty alcohol and an activated fatty acid. Dependent on the chain length and desaturation degree of the fatty acid and the fatty alcohol moiety, WE can have diverse physicochemical properties. WE derived from monounsaturated long‐chain acyl moieties are of industrial interest due to their very good lubrication properties. Whereas WE were obtained in the past from spermaceti organs of the sperm whale, industrial WE are nowadays mostly produced chemically from fossil fuels. In order to produce WE more sustainably, attempts to produce industrial WE in transgenic plants are steadily increasing. To achieve this, different combinations of WE producing enzymes are expressed in developing Arabidopsis thaliana or Camelina sativa seeds. Here we report the identification and characterization of a fifth wax synthase from the organism Marinobacter aquaeolei VT8, MaWSD5. It belongs to the class of bifunctional wax synthase/acyl‐CoA:diacylglycerol O‐acyltransferases (WSD). The protein was purified to homogeneity. In vivo and in vitro substrate analyses revealed that MaWSD5 is able to synthesize WE but no triacylglycerols. The protein produces WE from saturated and monounsaturated mid‐ and long‐chain substrates. Arabidopsis thaliana seeds expressing a fatty acid reductase from Marinobacter aquaeolei VT8 and MaWSD5 produce WE. Main WE synthesized are 20:1/18:1 and 20:1/20:1. This makes MaWSD5 a suitable candidate for industrial WE production in planta."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659"],["dc.identifier.doi","10.1002/lipd.12250"],["dc.identifier.eissn","1558-9307"],["dc.identifier.issn","0024-4201"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82106"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.publisher","John Wiley \\u0026 Sons, Inc."],["dc.relation.eissn","1558-9307"],["dc.relation.issn","0024-4201"],["dc.rights","This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited."],["dc.title","The Fifth WS/DGAT Enzyme of the Bacterium Marinobacter aquaeolei VT8"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.lastpage","16"],["dc.contributor.author","Miller, James"],["dc.contributor.author","Smyer Yu, Dan"],["dc.contributor.author","van der Veer, Peter"],["dc.contributor.editor","Smyer Yu, Dan"],["dc.contributor.editor","Miller, James R."],["dc.contributor.editor","van der Veer, Peter"],["dc.date.accessioned","2018-02-20T10:56:00Z"],["dc.date.available","2018-02-20T10:56:00Z"],["dc.date.issued","2014"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/12372"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.publisher","Routledge"],["dc.publisher.place","London"],["dc.relation.isbn","978-0-415-85515-0"],["dc.relation.ispartof","Religion and Ecological Sustainability in China"],["dc.title","Introduction: The diversity of eco-religious practice in China"],["dc.type","book_chapter"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","53"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.journal","Biotechnology for Biofuels"],["dc.bibliographiccitation.lastpage","14"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Yu, Dan"],["dc.contributor.author","Hornung, Ellen"],["dc.contributor.author","Iven, Tim"],["dc.contributor.author","Feussner, Ivo"],["dc.date.accessioned","2019-07-09T11:45:15Z"],["dc.date.available","2019-07-09T11:45:15Z"],["dc.date.issued","2018"],["dc.description.abstract","Background: Biotechnology enables the production of high-valued industrial feedstocks from plant seed oil. The plant-derived wax esters with long-chain monounsaturated acyl moieties, like oleyl oleate, have favorite properties for lubrication. For biosynthesis of wax esters using acyl-CoA substrates, expressions of a fatty acyl reductase (FAR) and a wax synthase (WS) in seeds are sufficient. Results: For optimization of the enzymatic activity and subcellular localization of wax ester synthesis enzymes, two fusion proteins were created, which showed wax ester-forming activities in Saccharomyces cerevisiae. To promote the formation of oleyl oleate in seed oil, WSs from Acinetobactor baylyi (AbWSD1) and Marinobacter aquaeolei (MaWS2), as well as the two created fusion proteins were tested in Arabidopsis to evaluate their abilities and substrate preference for wax ester production. The tested seven enzyme combinations resulted in different yields and compositions of wax esters. Expression of a FAR of Marinobacter aquaeolei (MaFAR) with AbWSD1 or MaWS2 led to a high incorporation of C18 substrates in wax esters. The MaFAR/TMMmAWAT2-AbWSD1 combination resulted in the incorporation of more C18:1 alcohol and C18:0 acyl moieties into wax esters compared with MaFAR/AbWSD1. The fusion protein of a WS from Simmondsia chinensis (ScWS) with MaFAR exhibited higher specificity toward C20:1 substrates in preference to C18:1 substrates. Expression of MaFAR/AbWSD1 in the Arabidopsis fad2 fae1 double mutant resulted in the accumulation of oleyl oleate (18:1/18:1) in up to 62 mol% of total wax esters in seed oil, which was much higher than the 15 mol% reached by MaFAR/AbWSD1 in Arabidopsis Col-0 background. In order to increase the level of oleyl oleate in seed oil of Camelina, lines expressing MaFAR/ScWS were crossed with a transgenic high oleate line. The resulting plants accumulated up to >40 mg g seed-1 of wax esters, containing 27-34 mol% oleyl oleate. Conclusions: The overall yields and the compositions of wax esters can be strongly affected by the availability of acyl-CoA substrates and to a lesser extent, by the characteristics of wax ester synthesis enzymes. For synthesis of oleyl oleate in plant seed oil, appropriate wax ester synthesis enzymes with high catalytic efficiency and desired substrate specificity should be expressed in plant cells; meanwhile, high levels of oleic acid-derived substrates need to be supplied to these enzymes by modifying the fatty acid profile of developing seeds."],["dc.description.abstract","Background: Biotechnology enables the production of high-valued industrial feedstocks from plant seed oil. The plant-derived wax esters with long-chain monounsaturated acyl moieties, like oleyl oleate, have favorite properties for lubrication. For biosynthesis of wax esters using acyl-CoA substrates, expressions of a fatty acyl reductase (FAR) and a wax synthase (WS) in seeds are sufficient. Results: For optimization of the enzymatic activity and subcellular localization of wax ester synthesis enzymes, two fusion proteins were created, which showed wax ester-forming activities in Saccharomyces cerevisiae. To promote the formation of oleyl oleate in seed oil, WSs from Acinetobactor baylyi (AbWSD1) and Marinobacter aquaeolei (MaWS2), as well as the two created fusion proteins were tested in Arabidopsis to evaluate their abilities and substrate preference for wax ester production. The tested seven enzyme combinations resulted in different yields and compositions of wax esters. Expression of a FAR of Marinobacter aquaeolei (MaFAR) with AbWSD1 or MaWS2 led to a high incorporation of C18 substrates in wax esters. The MaFAR/TMMmAWAT2-AbWSD1 combination resulted in the incorporation of more C18:1 alcohol and C18:0 acyl moieties into wax esters compared with MaFAR/AbWSD1. The fusion protein of a WS from Simmondsia chinensis (ScWS) with MaFAR exhibited higher specificity toward C20:1 substrates in preference to C18:1 substrates. Expression of MaFAR/AbWSD1 in the Arabidopsis fad2 fae1 double mutant resulted in the accumulation of oleyl oleate (18:1/18:1) in up to 62 mol% of total wax esters in seed oil, which was much higher than the 15 mol% reached by MaFAR/AbWSD1 in Arabidopsis Col-0 background. In order to increase the level of oleyl oleate in seed oil of Camelina, lines expressing MaFAR/ScWS were crossed with a transgenic high oleate line. The resulting plants accumulated up to >40 mg g seed-1 of wax esters, containing 27-34 mol% oleyl oleate. Conclusions: The overall yields and the compositions of wax esters can be strongly affected by the availability of acyl-CoA substrates and to a lesser extent, by the characteristics of wax ester synthesis enzymes. For synthesis of oleyl oleate in plant seed oil, appropriate wax ester synthesis enzymes with high catalytic efficiency and desired substrate specificity should be expressed in plant cells; meanwhile, high levels of oleic acid-derived substrates need to be supplied to these enzymes by modifying the fatty acid profile of developing seeds."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2018"],["dc.identifier.doi","10.1186/s13068-018-1057-4"],["dc.identifier.pmid","29507605"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15144"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59193"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","In goescholar not merged with http://resolver.sub.uni-goettingen.de/purl?gs-1/15080 but duplicate"],["dc.relation.issn","1754-6834"],["dc.rights","CC BY 4.0"],["dc.rights.access","openAccess"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","572"],["dc.title","High-level accumulation of oleyl oleate in plant seed oil by abundant supply of oleic acid substrates to efficient wax ester synthesis enzymes."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.contributor.author","Smyer Yu, Dan"],["dc.contributor.author","van der Veer, Peter"],["dc.contributor.editor","Smyer Yu, Dan"],["dc.contributor.editor","Faxiang, S"],["dc.date.accessioned","2018-02-20T10:55:59Z"],["dc.date.available","2018-02-20T10:55:59Z"],["dc.date.issued","2013"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/12369"],["dc.language.iso","zh"],["dc.notes.status","zu prüfen"],["dc.publisher","Minzu University Press"],["dc.publisher.place","Beijing"],["dc.relation.ispartof","Religious diversity and ecological sustainability in China == 中国宗教多元与生态可持续性"],["dc.title","Introduction: Diversity of belief systems and ecological practices in China == 中国信仰与生态实践的多元性}"],["dc.type","book_chapter"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]