Geisler, Claudia

[["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Immunobiology"],["dc.bibliographiccitation.volume","221"],["dc.contributor.author","Mazkereth, Niv"],["dc.contributor.author","Rocca, Francesco"],["dc.contributor.author","Schubert, Jennifer-Rose"],["dc.contributor.author","Geisler, Claudia"],["dc.contributor.author","Hillman, Yaron"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Fishelson, Zvi"],["dc.date.accessioned","2018-11-07T10:08:08Z"],["dc.date.available","2018-11-07T10:08:08Z"],["dc.date.issued","2016"],["dc.format.extent","1181"],["dc.identifier.doi","10.1016/j.imbio.2016.06.126"],["dc.identifier.isi","000382804300123"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/39412"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Gmbh, Urban & Fischer Verlag"],["dc.publisher.place","Jena"],["dc.relation.issn","0171-2985"],["dc.title","Membrane stability of the C5b-9 complexes: A nanoscopcial analysis of the interactions of C5b-9 with mortalin and caveolin-1"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","563"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Journal of the Royal Statistical Society: Series B (Statistical Methodology)"],["dc.bibliographiccitation.lastpage","587"],["dc.bibliographiccitation.volume","78"],["dc.contributor.author","Hartmann, Alexander K."],["dc.contributor.author","Huckemann, Stephan"],["dc.contributor.author","Dannemann, Jörn"],["dc.contributor.author","Laitenberger, Oskar"],["dc.contributor.author","Geisler, Claudia"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Munk, Axel"],["dc.date.accessioned","2017-09-07T11:44:53Z"],["dc.date.available","2017-09-07T11:44:53Z"],["dc.date.issued","2016"],["dc.description.abstract","A major challenge in many modern superresolution fluorescence microscopy techniques at the nanoscale lies in the correct alignment of long sequences of sparse but spatially and temporally highly resolved images. This is caused by the temporal drift of the protein structure, e.g. due to temporal thermal inhomogeneity of the object of interest or its supporting area during the observation process. We develop a simple semiparametric model for drift correction in single-marker switching microscopy. Then we propose an M-estimator for the drift and show its asymptotic normality. This is used to correct the final image and it is shown that this purely statistical method is competitive with state of the art calibration techniques which require the incorporation of fiducial markers in the specimen. Moreover, a simple bootstrap algorithm allows us to quantify the precision of the drift estimate and its effect on the final image estimation. We argue that purely statistical drift correction is even more robust than fiducial tracking, rendering the latter superfluous in many applications. The practicability of our method is demonstrated by a simulation study and by a single-marker switching application. This serves as a prototype for many other typical imaging techniques where sparse observations with high temporal resolution are blurred by motion of the object to be reconstructed."],["dc.identifier.doi","10.1111/rssb.12128"],["dc.identifier.gro","3141679"],["dc.identifier.isi","000376150200002"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/8562"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1467-9868"],["dc.relation.issn","1369-7412"],["dc.title","Drift estimation in sparse sequential dynamic imaging, with application to nanoscale fluorescence microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4516"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Optics Express"],["dc.bibliographiccitation.volume","28"],["dc.contributor.author","Vinçon, Britta"],["dc.contributor.author","Geisler, Claudia"],["dc.contributor.author","Egner, Alexander"],["dc.date.accessioned","2020-12-10T18:42:02Z"],["dc.date.available","2020-12-10T18:42:02Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1364/OE.385174"],["dc.identifier.pmid","32121686"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/77782"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/9"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.workinggroup","RG Egner"],["dc.title","Pixel hopping enables fast STED nanoscopy at low light dose"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3719"],["dc.bibliographiccitation.issue","19"],["dc.bibliographiccitation.journal","Cellular and Molecular Life Sciences"],["dc.bibliographiccitation.lastpage","3731"],["dc.bibliographiccitation.volume","73"],["dc.contributor.author","Gucek, Alenka"],["dc.contributor.author","Jorgacevski, Jernej"],["dc.contributor.author","Singh, Priyanka"],["dc.contributor.author","Geisler, Claudia"],["dc.contributor.author","Lisjak, Marjeta"],["dc.contributor.author","Vardjan, Nina"],["dc.contributor.author","Kreft, Marko"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Zorec, Robert"],["dc.date.accessioned","2018-11-07T10:08:12Z"],["dc.date.available","2018-11-07T10:08:12Z"],["dc.date.issued","2016"],["dc.description.abstract","Key support for vesicle-based release of gliotransmitters comes from studies of transgenic mice with astrocyte-specific expression of a dominant-negative domain of synaptobrevin 2 protein (dnSNARE). To determine how this peptide affects exocytosis, we used super-resolution stimulated emission depletion microscopy and structured illumination microscopy to study the anatomy of single vesicles in astrocytes. Smaller vesicles contained amino acid and peptidergic transmitters and larger vesicles contained ATP. Discrete increases in membrane capacitance, indicating single-vesicle fusion, revealed that astrocyte stimulation increases the frequency of predominantly transient fusion events in smaller vesicles, whereas larger vesicles transitioned to full fusion. To determine whether this reflects a lower density of SNARE proteins in larger vesicles, we treated astrocytes with botulinum neurotoxins D and E, which reduced exocytotic events of both vesicle types. dnSNARE peptide stabilized the fusion-pore diameter to narrow, release-unproductive diameters in both vesicle types, regardless of vesicle diameter."],["dc.identifier.doi","10.1007/s00018-016-2213-2"],["dc.identifier.isi","000382389500008"],["dc.identifier.pmid","27056575"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/39425"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","Basel"],["dc.relation.issn","1420-9071"],["dc.relation.issn","1420-682X"],["dc.title","Dominant negative SNARE peptides stabilize the fusion pore in a narrow, release-unproductive state"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3303"],["dc.bibliographiccitation.issue","16"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","3314"],["dc.bibliographiccitation.volume","120"],["dc.contributor.author","Siegmund, René"],["dc.contributor.author","Werner, Frank"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Geisler, Claudia"],["dc.contributor.author","Egner, Alexander"],["dc.date.accessioned","2021-10-01T09:57:28Z"],["dc.date.available","2021-10-01T09:57:28Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1016/j.bpj.2021.05.031"],["dc.identifier.pii","S0006349521005567"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/89845"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/322"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-469"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.issn","0006-3495"],["dc.relation.workinggroup","RG Egner"],["dc.relation.workinggroup","RG Jakobs (Structure and Dynamics of Mitochondria)"],["dc.title","isoSTED microscopy with water-immersion lenses and background reduction"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3139"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Biomedical Optics Express"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Krüger, Jennifer-Rose"],["dc.contributor.author","Keller-Findeisen, Jan"],["dc.contributor.author","Geisler, Claudia"],["dc.contributor.author","Egner, Alexander"],["dc.date.accessioned","2021-04-14T08:25:13Z"],["dc.date.available","2021-04-14T08:25:13Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1364/BOE.391787"],["dc.identifier.pmid","32637247"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/81559"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/183"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","2156-7085"],["dc.relation.issn","2156-7085"],["dc.relation.workinggroup","RG Egner"],["dc.title","Tomographic STED microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","784"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Molecular Reproduction and Development"],["dc.bibliographiccitation.lastpage","794"],["dc.bibliographiccitation.volume","73"],["dc.contributor.author","Rashid, S."],["dc.contributor.author","Breckle, R."],["dc.contributor.author","Hupe, M."],["dc.contributor.author","Geisler, S."],["dc.contributor.author","Doerwald, N."],["dc.contributor.author","Neesen, J."],["dc.date.accessioned","2018-11-07T09:46:54Z"],["dc.date.available","2018-11-07T09:46:54Z"],["dc.date.issued","2006"],["dc.description.abstract","Axonemal dyneins are large motor protein complexes generating the force for the movement of eukaryotic cilia and flagella. Disruption of axonemal dynein function leads to loss of ciliary motility and can result in male infertility or lateralization defects. Here, we report the molecular analysis of a murine gene encoding the dynein axonemal light intermediate chain Dnali1. The Dnali1 gene is localized on chromosome 4 and consists of six exons. It is predominantly expressed within the testis but at a lower level Dnali1 transcripts were also observed in different murine tissues, which exhibit cilia. Two transcript variants were detected, generated by the usage of two alternative polyadenylation signals within exon 6. Antibodies were raised against a GST-Dnali1 fusion protein and used to localize Dnali1 within differentiating male germ cells. Dnali1 is strongly expressed in spermatids but was also detected in spermatocytes. Moreover, the Dnali1 protein was localized in cilia of the trachea as well as in flagella of mature sperm supporting its function as an axonemal dynein. To identify putative Dnali1 interacting polypeptides, a yeast two-hybrid approach was performed using a murine testicular cDNA library. By this assay, the C-terminal part of the cytoplasmic dynein heavy chain 1 was identified as a putative interacting polypeptide of Dnali1. The interaction between the axonemal and the cytoplasmic dynein fragments was proven by co-immuno and co-localization experiments."],["dc.identifier.doi","10.1002/mrd.20475"],["dc.identifier.isi","000237128200013"],["dc.identifier.pmid","16496424"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/34990"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-liss"],["dc.relation.issn","1040-452X"],["dc.title","The murine Dnali1 gene encodes a flagellar protein that interacts with the cytoplasmic dynein heavy chain 1"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","108"],["dc.contributor.author","Gucek, Alenka"],["dc.contributor.author","Jorgacevski, Jernej"],["dc.contributor.author","Singh, Priyanka"],["dc.contributor.author","Geisler, Claudia"],["dc.contributor.author","Vardjan, Nina"],["dc.contributor.author","Kreft, Marko"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Zorec, Robert"],["dc.date.accessioned","2018-11-07T10:01:55Z"],["dc.date.available","2018-11-07T10:01:55Z"],["dc.date.issued","2015"],["dc.format.extent","102A"],["dc.identifier.isi","000359471700515"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38129"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.publisher.place","Cambridge"],["dc.relation.issn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.title","Fusion Properties of Gliotransmitter Vesicles in Cultured Astrocytes"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","106"],["dc.contributor.author","Gucek, Alenka"],["dc.contributor.author","Jorgacevski, Jernej"],["dc.contributor.author","Singh, Priyanka"],["dc.contributor.author","Geisler, Claudia"],["dc.contributor.author","Rituper, Bostjan"],["dc.contributor.author","Vardjan, Nina"],["dc.contributor.author","Kreft, Marko"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Zorec, Robert"],["dc.date.accessioned","2018-11-07T09:44:57Z"],["dc.date.available","2018-11-07T09:44:57Z"],["dc.date.issued","2014"],["dc.format.extent","526A"],["dc.identifier.isi","000337000403007"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/34512"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.publisher.place","Cambridge"],["dc.relation.eventlocation","San Francisco, CA"],["dc.relation.issn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.title","Fusion Properties of Gliotransmitter Vesicles in Astrocytes"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","E144"],["dc.bibliographiccitation.journal","Glia"],["dc.bibliographiccitation.lastpage","E145"],["dc.bibliographiccitation.volume","65"],["dc.contributor.author","Jorgacevski, J."],["dc.contributor.author","Kreft, Marko"],["dc.contributor.author","Geisler, Claudia"],["dc.contributor.author","Potokar, M."],["dc.contributor.author","Singh, P."],["dc.contributor.author","Gucek, Alenka"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Zorec, Robert"],["dc.date.accessioned","2018-11-07T10:23:03Z"],["dc.date.available","2018-11-07T10:23:03Z"],["dc.date.issued","2017"],["dc.description.sponsorship","Slovenian Research Agency [P3 0310, J3 3632, J3 4146, J3 7605]"],["dc.identifier.isi","000403071700216"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/42386"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Wiley"],["dc.publisher.place","Hoboken"],["dc.relation.conference","13th European Meeting on Glial Cells in Health and Disease"],["dc.relation.eventlocation","Edinburgh, SCOTLAND"],["dc.relation.issn","1098-1136"],["dc.relation.issn","0894-1491"],["dc.title","The anatomy of astrocytic vesicles containing gliotransmitters"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]