Kramer, Corinna

[["dc.bibliographiccitation.firstpage","126a"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","110"],["dc.contributor.author","Schön, Markus"],["dc.contributor.author","Kramer, Corinna"],["dc.contributor.author","Noeding, Helen"],["dc.contributor.author","Mey, Ingo"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2020-12-10T14:22:43Z"],["dc.date.available","2020-12-10T14:22:43Z"],["dc.date.issued","2016"],["dc.format.extent","126A"],["dc.identifier.doi","10.1016/j.bpj.2015.11.727"],["dc.identifier.isi","000375093800128"],["dc.identifier.issn","0006-3495"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71703"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.publisher.place","Cambridge"],["dc.relation.eventlocation","Los Angeles, CA"],["dc.relation.issn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.title","Self-Organization of Actomyosin Networks Attached to Artificial Membranes"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2710"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","2719"],["dc.bibliographiccitation.volume","110"],["dc.contributor.author","Shabardina, Victoria"],["dc.contributor.author","Kramer, Corinna"],["dc.contributor.author","Gerdes, Benjamin"],["dc.contributor.author","Braunger, Julia A."],["dc.contributor.author","Cordes, Andrea"],["dc.contributor.author","Schäfer, Jonas"],["dc.contributor.author","Mey, Ingo"],["dc.contributor.author","Grill, David"],["dc.contributor.author","Gerke, Volker"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2017-09-07T11:44:51Z"],["dc.date.available","2017-09-07T11:44:51Z"],["dc.date.issued","2016"],["dc.description.abstract","Ezrin, a protein of the ezrin, radixin, moesin (ERM) family, provides a regulated linkage between the plasma membrane and the cytoskeleton. The hallmark of this linkage is the activation of ezrin by phosphatidylinositol-4,5-bisphosphate (PIP2) binding and a threonine phosphorylation at position 567. To analyze the influence of these activating factors on the organization of ezrin on lipid membranes and the proposed concomitant oligomer-monomer transition, we made use of supported lipid bilayers in conjunction with atomic force microscopy and fluorescence microscopy. Bilayers doped with either PIP2 as the natural receptor lipid of ezrin or a Ni-nitrilotriacetic acid-equipped lipid to bind the proteins via their His6-tags to the lipid membrane were used to bind two different ezrin variants: ezrin wild-type and ezrin T567D mimicking the phosphorylated state. Using a combination of reflectometric interference spectroscopy, atomic force microscopy, and Forster resonance energy transfer experiments, we show that only the ezrin T567D mutant, upon binding to PIP2-containing bilayers, undergoes a remarkable conformational change, which we attribute to an opening of the conformation resulting in monomeric protein on the lipid bilayer."],["dc.identifier.doi","10.1016/j.bpj.2016.05.009"],["dc.identifier.gro","3141663"],["dc.identifier.isi","000378383300018"],["dc.identifier.pmid","27332129"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/6786"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.title","Mode of Ezrin-Membrane Interaction as a Function of PIP2 Binding and Pseudophosphorylation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","694"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Laryngo-Rhino-Otologie"],["dc.bibliographiccitation.lastpage","706"],["dc.bibliographiccitation.volume","99"],["dc.contributor.author","Stöver, T."],["dc.contributor.author","Dazert, S."],["dc.contributor.author","Hoffmann, T. K."],["dc.contributor.author","Plontke, S. K."],["dc.contributor.author","Ambrosch, P."],["dc.contributor.author","Arens, C."],["dc.contributor.author","Betz, C."],["dc.contributor.author","Beutner, D."],["dc.contributor.author","Bohr, C."],["dc.contributor.author","Bruchhage, K.-L."],["dc.contributor.author","Canis, M."],["dc.contributor.author","Dietz, A."],["dc.contributor.author","Guntinas-Lichius, O."],["dc.contributor.author","Hagen, R."],["dc.contributor.author","Hosemann, W."],["dc.contributor.author","Iro, H."],["dc.contributor.author","Klussmann, J.-P."],["dc.contributor.author","Knopf, A."],["dc.contributor.author","Kramer, S."],["dc.contributor.author","Lang, S."],["dc.contributor.author","Leinung, M."],["dc.contributor.author","Lenarz, T."],["dc.contributor.author","Löwenheim, H."],["dc.contributor.author","Matthias, C."],["dc.contributor.author","Mlynski, R."],["dc.contributor.author","Olze, H."],["dc.contributor.author","Park, J."],["dc.contributor.author","Plinkert, P."],["dc.contributor.author","Radeloff, A."],["dc.contributor.author","Rotter, N."],["dc.contributor.author","Rudack, C."],["dc.contributor.author","Bozzato, A."],["dc.contributor.author","Schipper, J."],["dc.contributor.author","Schrader, M."],["dc.contributor.author","Strieth, S."],["dc.contributor.author","Stuck, B. A."],["dc.contributor.author","Volkenstein, S."],["dc.contributor.author","Westhofen, M."],["dc.contributor.author","Wolf, G."],["dc.contributor.author","Wollenberg, B."],["dc.contributor.author","Zahnert, T."],["dc.contributor.author","Zenk, J."],["dc.date.accessioned","2021-04-14T08:23:07Z"],["dc.date.available","2021-04-14T08:23:07Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1055/a-1232-4911"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80799"],["dc.language.iso","de"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1438-8685"],["dc.relation.issn","0935-8943"],["dc.title","Auswirkungen der SARS-CoV-2-Pandemie auf die universitäre Hals-Nasen-Ohrenheilkunde im Bereich der Krankenversorgung"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1987"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Analyst"],["dc.bibliographiccitation.lastpage","1992"],["dc.bibliographiccitation.volume","139"],["dc.contributor.author","Stephan, Milena"],["dc.contributor.author","Kramer, Corinna"],["dc.contributor.author","Steinem, Claudia"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2017-09-07T11:46:55Z"],["dc.date.available","2017-09-07T11:46:55Z"],["dc.date.issued","2014"],["dc.description.abstract","Small molecule sensing is of great importance in pharmaceutical research. While there exist well established screening methods such as EMSA (electrophoretic motility shift assay) or biointeraction chromatography to report on successful binding interactions, there are only a few techniques that allow studying and quantifying the interaction of low molecular weight analytes with a binding partner directly. We report on a binding assay for small molecules based on the reflectivity change of a porous transparent film upon immobilisation of an absorbing substance on the pore walls. The porous matrix acts as a thin optical transparent film to produce interference fringes and accumulates molecules at the inner wall to amplify the sensor response. The benefits and limits of the assay are demonstrated by investigating the binding of biotin labelled with an atto dye to avidin physisorbed within an anodic aluminium oxide membrane."],["dc.identifier.doi","10.1039/c4an00009a"],["dc.identifier.fs","604886"],["dc.identifier.gro","3142213"],["dc.identifier.isi","000333081800024"],["dc.identifier.pmid","24599267"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11469"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/5788"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: DFG [SFB 937 (A08)]"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1364-5528"],["dc.relation.issn","0003-2654"],["dc.rights.access","openAccess"],["dc.title","Binding assay for low molecular weight analytes based on reflectometry of absorbing molecules in porous substrates"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","94"],["dc.bibliographiccitation.issue","1-2"],["dc.bibliographiccitation.journal","Geoderma"],["dc.bibliographiccitation.lastpage","105"],["dc.bibliographiccitation.volume","128"],["dc.contributor.author","Rethemeyer, Janet"],["dc.contributor.author","Kramer, C."],["dc.contributor.author","Gleixner, Gerd"],["dc.contributor.author","John, Bettina"],["dc.contributor.author","Yamashita, T."],["dc.contributor.author","Flessa, H."],["dc.contributor.author","Andersen, N."],["dc.contributor.author","Nadeau, M. J."],["dc.contributor.author","Grootes, Pieter M."],["dc.date.accessioned","2018-11-07T10:56:10Z"],["dc.date.available","2018-11-07T10:56:10Z"],["dc.date.issued","2005"],["dc.description.abstract","Radiocarbon measured by accelerator mass spectrometry (AMS) was used as a tracer to study the origin and fate of organic materials in soils. Fractionation methods used to separate the heterogeneous organic material into functionally defined pools of different stability included acid-alkali-acid extraction and density fractionation. (14)C values of the humin fraction, isolated from samples of different field trials at the agricultural station Rotthalmunster (Germany), yielded (14)C decreases of about 30% to 54% from the surface soil to ca. 65 cm depth. These results indicate a progressive enrichment of stable organic compounds with increasing soil depth. In contrast, a minor decline in 14C concentrations of the humic acid fraction, which mostly showed higher 14C values than the humin, reflect the translocation of modern organic carbon towards greater depth. Low radiocarbon levels of the light occluded particulate organic matter (< 1.6 g/cm(3)), obtained by density separation, suggest stabilization of organic carbon in soil aggregates. A comparison of 14C results for density fractions from field trials located in a rural and an industrialized region reflect their susceptibility to contamination by fossil fuel-derived carbon and their heterogeneous composition. As a consequence individual short-chain phospholipid fatty acids (PLFA), as indicators for viable soil microbial biomass, were isolated by preparative capillary gas-chromatography. Compound-specific radiocarbon analysis of the isolated PLFAs revealed the assimilation of different substrates for their synthesis. (14)C concentrations of the monounsaturated PLFAs (n-C16:1, n-C17:1, and n-C18:1), which were close to the modern atmospheric (14)C level, suggest a high specificity to young carbon sources. The saturated PLFAs, isolated from the plough-horizon, were synthesized from sub-recent soil organic carbon (SOC) as shown by a higher contribution of bomb-(14)C. A considerable 14C decrease from the surface to 30-45 cm soil depth of the saturated PLFAs indicates the incorporation of more stabilized SOC particularly in subsoil i/a-C15:0, n-C16:0, and 17C17:0 PLFAs. (c) 2004 Elsevier B.V. All rights reserved."],["dc.identifier.doi","10.1016/j.geoderma.2004.12.017"],["dc.identifier.isi","000230804300009"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/49950"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.publisher.place","Amsterdam"],["dc.relation.conference","International Conference on Mechanisms and Regulation of Organic Matter in Soils"],["dc.relation.eventlocation","Schloss Hohenkammer, GERMANY"],["dc.relation.issn","0016-7061"],["dc.title","Transformation of organic matter in agricultural soils: radiocarbon concentration versus soil depth"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","European Biophysics Journal"],["dc.bibliographiccitation.volume","42"],["dc.contributor.author","Kramer, C."],["dc.contributor.author","Morick, Daniela"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2018-11-07T09:22:33Z"],["dc.date.available","2018-11-07T09:22:33Z"],["dc.date.issued","2013"],["dc.format.extent","S152"],["dc.identifier.isi","000330215300448"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29370"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","New york"],["dc.relation.eventlocation","Lisbon, Portugal"],["dc.relation.issn","1432-1017"],["dc.relation.issn","0175-7571"],["dc.title","Cytoskeletal interaction forces characterized by means of atomic force microscopy"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","14204"],["dc.bibliographiccitation.issue","46"],["dc.bibliographiccitation.journal","Langmuir"],["dc.bibliographiccitation.lastpage","14213"],["dc.bibliographiccitation.volume","29"],["dc.contributor.author","Braunger, Julia A."],["dc.contributor.author","Kramer, Corinna"],["dc.contributor.author","Morick, Daniela"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2017-09-07T11:47:02Z"],["dc.date.available","2017-09-07T11:47:02Z"],["dc.date.issued","2013"],["dc.description.abstract","Phosphoinositides and in particular L-alpha-phosphatidylinositol-4,5-bisphosphate (PIP2) are key lipids controlling many cellular events and serve as receptors for a large number of intracellular proteins. To quantitatively analyze protein-PIP2 interactions in vitro in a time-resolved manner, planar membranes on solid substrates are highly desirable. Here, we describe an optimized protocol to form PIP2 containing planar solid supported membranes on silicon surfaces by vesicle spreading. Supported lipid bilayers (SLBs) were obtained by spreading POPC/PIP2 (92:8) small unilamellar vesicles onto hydrophilic silicon substrates at a low pH of 4.8. These membranes were capable of binding ezrin, resulting in large protein coverage as concluded from reflectometric interference spectroscopy and fluorescence microscopy. As deduced from fluorescence microscopy, only under low pH conditions, a homogeneously appearing distribution of fluorescently labeled PIP2 molecules in the membrane was achieved. Fluorescence recovery after photobleaching experiments revealed that PIP2 is not mobile in the bottom layer of the SLBs, while PIP2 is fully mobile in the top layer with diffusion coefficients of about 3 mu m(2)/s. This diffusion coefficient was considerably reduced by a factor of about 3 if ezrin has been bound to PIP2 in the membrane."],["dc.identifier.doi","10.1021/la402646k"],["dc.identifier.gro","3142249"],["dc.identifier.isi","000330144100026"],["dc.identifier.pmid","24199623"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/6187"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: DFG [STE 884/11-1]"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0743-7463"],["dc.title","Solid Supported Membranes Doped with PIP2: Influence of Ionic Strength and pH on Bilayer Formation and Membrane Organization"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","European Biophysics Journal"],["dc.bibliographiccitation.volume","42"],["dc.contributor.author","Steinem, Claudia"],["dc.contributor.author","Braunger, Julia A."],["dc.contributor.author","Kramer, C."],["dc.contributor.author","Gerke, V."],["dc.date.accessioned","2018-11-07T09:22:35Z"],["dc.date.available","2018-11-07T09:22:35Z"],["dc.date.issued","2013"],["dc.format.extent","S156"],["dc.identifier.isi","000330215300465"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29381"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","New york"],["dc.relation.eventlocation","Lisbon, Portugal"],["dc.relation.issn","1432-1017"],["dc.relation.issn","0175-7571"],["dc.title","Activation of ezrin: synergism of PIP2 interaction and phosphorylation"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","106"],["dc.contributor.author","Kramer, Corinna"],["dc.contributor.author","Morick, Daniela"],["dc.contributor.author","Mey, Ingo"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2018-11-07T09:44:57Z"],["dc.date.available","2018-11-07T09:44:57Z"],["dc.date.issued","2014"],["dc.format.extent","500A"],["dc.identifier.isi","000337000402769"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/34511"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.publisher.place","Cambridge"],["dc.relation.eventlocation","San Francisco, CA"],["dc.relation.issn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.title","Mechanics of F-Actin-Membrane Composites Investigated by Atomic Force Microscopy"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","HNO"],["dc.contributor.author","Stöver, T."],["dc.contributor.author","Dazert, S."],["dc.contributor.author","Plontke, S. K."],["dc.contributor.author","Kramer, S."],["dc.contributor.author","Ambrosch, P."],["dc.contributor.author","Arens, C."],["dc.contributor.author","Betz, C."],["dc.contributor.author","Beutner, D."],["dc.contributor.author","Bohr, C."],["dc.contributor.author","Bruchhage, K.-L."],["dc.contributor.author","Canis, M."],["dc.contributor.author","Dietz, A."],["dc.contributor.author","Guntinas-Lichius, O."],["dc.contributor.author","Hagen, R."],["dc.contributor.author","Hosemann, W."],["dc.contributor.author","Iro, H."],["dc.contributor.author","Klussmann, J. P"],["dc.contributor.author","Knopf, A."],["dc.contributor.author","Lang, S."],["dc.contributor.author","Leinung, M."],["dc.contributor.author","Lenarz, T."],["dc.contributor.author","Löwenheim, H."],["dc.contributor.author","Matthias, C."],["dc.contributor.author","Mlynski, R."],["dc.contributor.author","Olze, H."],["dc.contributor.author","Park, J."],["dc.contributor.author","Plinkert, P."],["dc.contributor.author","Radeloff, A."],["dc.contributor.author","Rotter, N."],["dc.contributor.author","Rudack, C."],["dc.contributor.author","Bozzato, A."],["dc.contributor.author","Schipper, J."],["dc.contributor.author","Schrader, M."],["dc.contributor.author","Schuler, P. J."],["dc.contributor.author","Strieth, S."],["dc.contributor.author","Stuck, B. A."],["dc.contributor.author","Volkenstein, S."],["dc.contributor.author","Westhofen, M."],["dc.contributor.author","Wolf, G."],["dc.contributor.author","Wollenberg, B."],["dc.contributor.author","Zahnert, T."],["dc.contributor.author","Zenk, J."],["dc.contributor.author","Hoffmann, T. K."],["dc.date.accessioned","2021-04-14T08:30:43Z"],["dc.date.available","2021-04-14T08:30:43Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1007/s00106-021-01001-8"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83347"],["dc.language.iso","de"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1433-0458"],["dc.relation.issn","0017-6192"],["dc.title","Auswirkungen der SARS-CoV‑2-Pandemie auf die universitäre Hals-Nasen-Ohren-Heilkunde im Bereich der Forschung, Lehre und Weiterbildung"],["dc.title.translated","Effects of the SARS-CoV‑2 pandemic on the otolaryngology university hospitals in the field of research, student teaching and specialist training"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]