Hirrlinger, Johannes

[["dc.bibliographiccitation.firstpage","1229"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","European Journal of Neuroscience"],["dc.bibliographiccitation.lastpage","1241"],["dc.bibliographiccitation.volume","37"],["dc.contributor.author","Rahman, Jamilur"],["dc.contributor.author","Latal, A. Tobias"],["dc.contributor.author","Besser, Stefanie"],["dc.contributor.author","Hirrlinger, Johannes"],["dc.contributor.author","Huelsmann, Swen"],["dc.date.accessioned","2018-11-07T09:26:26Z"],["dc.date.available","2018-11-07T09:26:26Z"],["dc.date.issued","2013"],["dc.description.abstract","Inhibitory neurons are involved in the generation and patterning of the respiratory rhythm in the adult animal. However, the role of glycinergic neurons in the respiratory rhythm in the developing network is still not understood. Although the complete loss of glycinergic transmission in vivo is lethal, the blockade of glycinergic transmission in slices of the medulla has little effect on pre-Botzinger complex network activity. As 50% of the respiratory rhythmic neurons in this slice preparation are glycinergic, they have to be considered as integrated parts of the network. We aimed to investigate whether glycinergic neurons receive mixed miniature inhibitory postsynaptic currents (mIPSCs) that result from co-release of GABA and glycine. Quantification of mixed mIPSCs by the use of different objective detection methods resulted in a wide range of results. Therefore, we generated traces of mIPSCs with a known distribution of mixed mIPSCs and mono-transmitter-induced mIPSCs, and tested the detection methods on the simulated data. We found that analysis paradigms, which are based on fitting the sum of two mIPSC templates, to be most acceptable. On the basis of these protocols, 2040% of all mIPSCs recorded from respiratory glycinergic neurons are mixed mIPSCs that result from co-release of GABA and glycine. Furthermore, single-cell reverse transcriptase polymerase chain reaction revealed that 46% of glycinergic neurons co-express mRNA of glycine transporter 2 together with at least one marker protein of GABAergic neurons. Our data suggest that significant co-transmission occurs in the pre-Botzinger complex that might be involved in the shaping of synaptic inhibition of respiratory glycinergic neurons."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft (DFG) [HI1414/2-1, HU797/7-1]; CMPB"],["dc.identifier.doi","10.1111/ejn.12136"],["dc.identifier.isi","000317850800003"],["dc.identifier.pmid","23347272"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/30298"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","0953-816X"],["dc.title","Mixed miniature postsynaptic currents resulting from co-release of glycine and GABA recorded from glycinergic neurons in the neonatal respiratory network"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","e3000943"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","PLoS Biology"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Trevisiol, Andrea"],["dc.contributor.author","Kusch, Kathrin"],["dc.contributor.author","Steyer, Anna M."],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Nardis, Christos"],["dc.contributor.author","Winkler, Ulrike"],["dc.contributor.author","Köhler, Susanne"],["dc.contributor.author","Restrepo, Alejandro"],["dc.contributor.author","Möbius, Wiebke"],["dc.contributor.author","Werner, Hauke B."],["dc.contributor.author","Nave, Klaus-Armin"],["dc.contributor.author","Hirrlinger, Johannes"],["dc.date.accessioned","2021-04-14T08:31:16Z"],["dc.date.available","2021-04-14T08:31:16Z"],["dc.date.issued","2020"],["dc.description.abstract","In several neurodegenerative disorders, axonal pathology may originate from impaired oligodendrocyte-to-axon support of energy substrates. We previously established transgenic mice that allow measuring axonal ATP levels in electrically active optic nerves. Here, we utilize this technique to explore axonal ATP dynamics in the Plpnull/y mouse model of spastic paraplegia. Optic nerves from Plpnull/y mice exhibited lower and more variable basal axonal ATP levels and reduced compound action potential (CAP) amplitudes, providing a missing link between axonal pathology and a role of oligodendrocytes in brain energy metabolism. Surprisingly, when Plpnull/y optic nerves are challenged with transient glucose deprivation, both ATP levels and CAP decline slower, but recover faster upon reperfusion of glucose. Structurally, myelin sheaths display an increased frequency of cytosolic channels comprising glucose and monocarboxylate transporters, possibly facilitating accessibility of energy substrates to the axon. These data imply that complex metabolic alterations of the axon–myelin unit contribute to the phenotype of Plpnull/y mice."],["dc.identifier.doi","10.1371/journal.pbio.3000943"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83539"],["dc.identifier.url","https://for2848.gwdguser.de/literature/publications/20"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","FOR 2848: Architektur und Heterogenität der inneren mitochondrialen Membran auf der Nanoskala"],["dc.relation","FOR 2848 | P08: Strukturelle und funktionale Veränderungen der inneren mitochondrialen Membran axonaler Mitochondrien in vivo in einem dymyelinisierenden Mausmodell"],["dc.relation.eissn","1545-7885"],["dc.relation.workinggroup","RG Möbius"],["dc.rights","CC BY 4.0"],["dc.title","Structural myelin defects are associated with low axonal ATP levels but rapid recovery from energy deprivation in a mouse model of spastic paraplegia"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","5687"],["dc.bibliographiccitation.issue","23"],["dc.bibliographiccitation.journal","The Journal of Physiology"],["dc.bibliographiccitation.lastpage","5705"],["dc.bibliographiccitation.volume","597"],["dc.contributor.author","Gerkau, Niklas J."],["dc.contributor.author","Lerchundi, Rodrigo"],["dc.contributor.author","Nelson, Joel S. E."],["dc.contributor.author","Lantermann, Marina"],["dc.contributor.author","Meyer, Jan"],["dc.contributor.author","Hirrlinger, Johannes"],["dc.contributor.author","Rose, Christine R."],["dc.date.accessioned","2021-06-01T10:47:31Z"],["dc.date.available","2021-06-01T10:47:31Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1113/JP278658"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85629"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","1469-7793"],["dc.relation.issn","0022-3751"],["dc.title","Relation between activity‐induced intracellular sodium transients and ATP dynamics in mouse hippocampal neurons"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1358"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Journal of Neuroscience"],["dc.bibliographiccitation.lastpage","1365"],["dc.bibliographiccitation.volume","24"],["dc.contributor.author","Grass, D."],["dc.contributor.author","Pawlowski, P. G."],["dc.contributor.author","Hirrlinger, Johannes"],["dc.contributor.author","Papadopoulos, Nestoras"],["dc.contributor.author","Richter, Diethelm W."],["dc.contributor.author","Kirchhoff, Frank"],["dc.contributor.author","Hulsmann, S."],["dc.date.accessioned","2018-11-07T10:51:10Z"],["dc.date.available","2018-11-07T10:51:10Z"],["dc.date.issued","2004"],["dc.description.abstract","A population of neurons in the caudal medulla generates the rhythmic activity underlying breathing movements. Although this neuronal network has attracted great attention for studying neuronal aspects of synaptic transmission, functions of glial cells supporting this neuronal activity remain unclear. To investigate the role of astrocytes in the respiratory network, we applied electrophysiological and immunohistochemical techniques to characterize astrocytes in regions involved in the generation and transmission of rhythmic activity. In the ventral respiratory group and the hypoglossal nucleus (XII) of acutely isolated brainstem slices, we analyzed fluorescently labeled astrocytes obtained from TgN(GFAP-EGFP) transgenic mice with the whole-cell voltage-clamp technique. Three subpopulations of astrocytes could be discerned by their distinct membrane current profiles. A first group of astrocytes was characterized by nonrectifying, symmetrical and voltage-independent potassium currents and a robust glutamate transporter response to D-aspartate. A second group of astrocytes showed additional A-type potassium currents, whereas a third group, identified by immunolabeling for the glial progenitor marker NG2, expressed outwardly rectifying potassium currents, smaller potassium inward currents, and only minimal D-aspartate-induced transporter currents. Astrocytes of all groups showed kainate-induced inward currents. We conclude that most of the astrocytes serve as a buffer system of excess extracellular glutamate and potassium; however, a distinct cell population (NG2-positive, A-type potassium currents) may play an important role for network plasticity."],["dc.identifier.doi","10.1523/JNEUROSCI.4022-03.2004"],["dc.identifier.isi","000188896100012"],["dc.identifier.pmid","14960607"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/48825"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Soc Neuroscience"],["dc.relation.issn","0270-6474"],["dc.title","Diversity of functional astroglial properties in the respiratory network"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","S23"],["dc.bibliographiccitation.journal","NEURON GLIA BIOLOGY"],["dc.bibliographiccitation.lastpage","S24"],["dc.bibliographiccitation.volume","2"],["dc.contributor.author","Dibaj, Payam"],["dc.contributor.author","Kaiser, M."],["dc.contributor.author","Hirrlinger, Johannes"],["dc.contributor.author","Kirchhoff, Frank"],["dc.contributor.author","Neusch, C."],["dc.date.accessioned","2018-11-07T11:07:10Z"],["dc.date.available","2018-11-07T11:07:10Z"],["dc.date.issued","2007"],["dc.identifier.isi","000251708800070"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52492"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cambridge Univ Press"],["dc.publisher.place","New york"],["dc.relation.issn","1740-925X"],["dc.title","Co-enrichment of Kir4.1 and AQP4 channels in spinal cord astrocytes suggests coupling of K+ flux and water transport: swelling experiments using transgenic mouse technology and time lapse 2-photon laser microscopy"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","EBC20220091"],["dc.bibliographiccitation.journal","Essays in Biochemistry"],["dc.contributor.author","Bohmbach, Kirsten"],["dc.contributor.author","Henneberger, Christian"],["dc.contributor.author","Hirrlinger, Johannes"],["dc.date.accessioned","2022-10-04T10:21:27Z"],["dc.date.available","2022-10-04T10:21:27Z"],["dc.date.issued","2022"],["dc.description.abstract","Abstract\n Learning and memory are fundamental but highly complex functions of the brain. They rely on multiple mechanisms including the processing of sensory information, memory formation, maintenance of short- and long-term memory, memory retrieval and memory extinction. Recent experiments provide strong evidence that, besides neurons, astrocytes crucially contribute to these higher brain functions. However, the complex interplay of astrocytes and neurons in local neuron–glia assemblies is far from being understood. Although important basic cellular principles that govern and link neuronal and astrocytic cellular functions have been established, additional mechanisms clearly continue to emerge. In this short essay, we first review current technologies allowing the experimenter to explore the role of astrocytes in behaving animals, with focus on spatial memory. We then discuss astrocytic signaling mechanisms and their role in learning and memory. We also reveal gaps in our knowledge that currently prevent a comprehensive understanding of how astrocytes contribute to acquisition, storage and retrieval of memory by modulating neuronal signaling in local circuits."],["dc.identifier.doi","10.1042/EBC20220091"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/114412"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-600"],["dc.relation.eissn","1744-1358"],["dc.relation.issn","0071-1365"],["dc.title","Astrocytes in memory formation and maintenance"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e110310"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Baier, Paul Christian"],["dc.contributor.author","Brzozka, Magdalena M."],["dc.contributor.author","Shahmoradi, Ali"],["dc.contributor.author","Reinecke, Lisa"],["dc.contributor.author","Kroos, Christina"],["dc.contributor.author","Wichert, Sven P."],["dc.contributor.author","Oster, Henrik"],["dc.contributor.author","Wehr, Michael C."],["dc.contributor.author","Taneja, Reshma"],["dc.contributor.author","Hirrlinger, Johannes"],["dc.contributor.author","Rossner, Moritz J."],["dc.date.accessioned","2018-11-07T09:33:26Z"],["dc.date.available","2018-11-07T09:33:26Z"],["dc.date.issued","2014"],["dc.description.abstract","Increasing evidence suggests that clock genes may be implicated in a spectrum of psychiatric diseases, including sleep and mood related disorders as well as schizophrenia. The bHLH transcription factors SHARP1/DEC2/BHLHE41 and SHARP2/DEC1/ BHLHE40 are modulators of the circadian system and SHARP1/DEC2/BHLHE40 has been shown to regulate homeostatic sleep drive in humans. In this study, we characterized Sharp1 and Sharp2 double mutant mice (S1/2(-/-)) using online EEG recordings in living animals, behavioral assays and global gene expression profiling. EEG recordings revealed attenuated sleep/wake amplitudes and alterations of theta oscillations. Increased sleep in the dark phase is paralleled by reduced voluntary activity and cortical gene expression signatures reveal associations with psychiatric diseases. S1/2(-/-) mice display alterations in novelty induced activity, anxiety and curiosity. Moreover, mutant mice exhibit impaired working memory and deficits in prepulse inhibition resembling symptoms of psychiatric diseases. Network modeling indicates a connection between neural plasticity and clock genes, particularly for SHARP1 and PER1. Our findings support the hypothesis that abnormal sleep and certain (endo) phenotypes of psychiatric diseases may be caused by common mechanisms involving components of the molecular clock including SHARP1 and SHARP2."],["dc.identifier.doi","10.1371/journal.pone.0110310"],["dc.identifier.isi","000343662800029"],["dc.identifier.pmid","25340473"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11034"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31963"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/3.0"],["dc.title","Mice Lacking the Circadian Modulators SHARP1 and SHARP2 Display Altered Sleep and Mixed State Endophenotypes of Psychiatric Disorders"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Physiology"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Oke, Yoshihiko"],["dc.contributor.author","Miwakeichi, Fumikazu"],["dc.contributor.author","Oku, Yoshitaka"],["dc.contributor.author","Hirrlinger, Johannes"],["dc.contributor.author","Hülsmann, Swen"],["dc.date.accessioned","2020-12-10T18:44:37Z"],["dc.date.available","2020-12-10T18:44:37Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.3389/fphys.2018.01586"],["dc.identifier.eissn","1664-042X"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78533"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation.iserratumof","/handle/2/78530"],["dc.title","Corrigendum: Cell Type-Dependent Activation Sequence During Rhythmic Bursting in the PreBötzinger Complex in Respiratory Rhythmic Slices From Mice"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","erratum_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0129934"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Besser, Stefanie"],["dc.contributor.author","Sicker, Marit"],["dc.contributor.author","Marx, Grit"],["dc.contributor.author","Winkler, Ulrike"],["dc.contributor.author","Eulenburg, Volker"],["dc.contributor.author","Huelsmann, Swen"],["dc.contributor.author","Hirrlinger, Johannes"],["dc.date.accessioned","2018-11-07T09:55:52Z"],["dc.date.available","2018-11-07T09:55:52Z"],["dc.date.issued","2015"],["dc.description.abstract","GABAergic inhibitory neurons are a large population of neurons in the central nervous system (CNS) of mammals and crucially contribute to the function of the circuitry of the brain. To identify specific cell types and investigate their functions labelling of cell populations by transgenic expression of fluorescent proteins is a powerful approach. While a number of mouse lines expressing the green fluorescent protein (GFP) in different subpopulations of GABAergic cells are available, GFP expressing mouse lines are not suitable for either crossbreeding to other mouse lines expressing GFP in other cell types or for Ca2+-imaging using the superior green Ca2+-indicator dyes. Therefore, we have generated a novel transgenic mouse line expressing the red fluorescent protein tdTomato in GABAergic neurons using a bacterial artificial chromosome based strategy and inserting the tdTomato open reading frame at the start codon within exon 1 of the GAD2 gene encoding glutamic acid decarboxylase 65 (GAD65). TdTomato expression was observed in all expected brain regions; however, the fluorescence intensity was highest in the olfactory bulb and the striatum. Robust expression was also observed in cortical and hippocampal neurons, Purkinje cells in the cerebellum, amacrine cells in the retina as well as in cells migrating along the rostral migratory stream. In cortex, hippocampus, olfactory bulb and brainstem, 80% to 90% of neurons expressing endogenous GAD65 also expressed the fluorescent protein. Moreover, almost all tdTomato-expressing cells coexpressed GAD65, indicating that indeed only GABAergic neurons are labelled by tdTomato expression. This mouse line with its unique spectral properties for labelling GABAergic neurons will therefore be a valuable new tool for research addressing this fascinating cell type."],["dc.description.sponsorship","\"Deutsche Forschungsgemeinschaft\" (DFG) [HI1414/2-1, HU797/7-1]"],["dc.identifier.doi","10.1371/journal.pone.0129934"],["dc.identifier.isi","000356329900114"],["dc.identifier.pmid","26076353"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11956"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36843"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","A Transgenic Mouse Line Expressing the Red Fluorescent Protein tdTomato in GABAergic Neurons"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","97"],["dc.bibliographiccitation.lastpage","100"],["dc.bibliographiccitation.seriesnr","669"],["dc.contributor.author","Winter, Stefan M."],["dc.contributor.author","Fresemann, Jens"],["dc.contributor.author","Schnell, Christian"],["dc.contributor.author","Oku, Yoshitaka"],["dc.contributor.author","Hirrlinger, Johannes"],["dc.contributor.author","Huelsmann, Swen"],["dc.date.accessioned","2018-11-07T08:48:16Z"],["dc.date.available","2018-11-07T08:48:16Z"],["dc.date.issued","2010"],["dc.description.abstract","The neuronal network in the pre-Botzinger Complex is the key element of respiratory rhythm generation. Isolated in a slice preparation, the pre-Botzinger Complex network is still able to generate its inspiratory activity. Although the mechanism of rhythm generation in principle relies on glutamatergic neurons, interestingly we found that glycinergic neurons represent a major portion of all inspiratory neurons in the slice preparation."],["dc.identifier.doi","10.1007/978-1-4419-5692-7_20"],["dc.identifier.isi","000277995200020"],["dc.identifier.pmid","20217329"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/21163"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","Berlin"],["dc.relation.conference","11th Oxford Conference on Modeling and Control of Breathing"],["dc.relation.crisseries","Advances in Experimental Medicine and Biology"],["dc.relation.eventend","2009-07-26"],["dc.relation.eventlocation","Nara, Japan"],["dc.relation.eventstart","2009-07-23"],["dc.relation.isbn","978-1-4419-5691-0"],["dc.relation.ispartof","New frontiers in respiratory control"],["dc.relation.ispartofseries","Advances in Experimental Medicine and Biology; 669"],["dc.title","Glycinergic Interneurons in the Respiratory Network of the Rhythmic Slice Preparation"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]