Vorbrüggen, Gerd

[["dc.bibliographiccitation.artnumber","dev.200465"],["dc.bibliographiccitation.journal","Development"],["dc.contributor.author","Rostam, Nadia"],["dc.contributor.author","Goloborodko, Alexander"],["dc.contributor.author","Riemer, Stephan"],["dc.contributor.author","Hertel, Andres"],["dc.contributor.author","Riedel, Dietmar"],["dc.contributor.author","Vorbrüggen, Gerd"],["dc.contributor.author","Dosch, Roland"],["dc.date.accessioned","2022-07-01T07:35:24Z"],["dc.date.available","2022-07-01T07:35:24Z"],["dc.date.issued","2022"],["dc.description.abstract","The zebrafish germline is specified during early embryogenesis by inherited maternal RNAs and proteins collectively called germ plasm. Only the cells containing germ plasm will become part of the germline, whereas the other cells will commit to somatic cell fates. Therefore, proper localization of germ plasm is key for germ cell specification and its removal is critical for the development of soma. The molecular mechanism underlying this process in vertebrates is largely unknown. Here we show that germ plasm localization in zebrafish is similar to Xenopus but distinct from Drosophila. We identified non muscle myosin II (NMII) and tight junction (TJ) components such as ZO2 and Claudin-d (Cldn-d) as interaction candidates of Bucky ball (Buc), which is the germ plasm organizer in zebrafish. Remarkably, we also found that TJ protein ZO1 colocalizes with germ plasm and electron microscopy (EM) of zebrafish embryos uncovered TJ like structures at the cleavage furrows where the germ plasm is anchored. In addition, injection of the TJ-receptor Cldn-d produced extra germ plasm aggregates whereas expression of a dominant negative version inhibits germ plasm aggregate formation. Our findings support for the first time a role of TJs in germ plasm localization."],["dc.identifier.doi","10.1242/dev.200465"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112157"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-581"],["dc.relation.eissn","1477-9129"],["dc.relation.issn","0950-1991"],["dc.title","The germ plasm is anchored at the cleavage furrows through interaction with tight junctions in the early zebrafish embryo"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Lee, Byung Cheon"],["dc.contributor.author","Lee, Hae Min"],["dc.contributor.author","Kim, Sorah"],["dc.contributor.author","Avanesov, Andrei S."],["dc.contributor.author","Lee, Aro"],["dc.contributor.author","Chun, Bok-Hwan"],["dc.contributor.author","Vorbruggen, Gerd"],["dc.contributor.author","Gladyshev, Vadim N."],["dc.date.accessioned","2020-12-10T18:10:07Z"],["dc.date.available","2020-12-10T18:10:07Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1038/s41598-017-15090-5"],["dc.identifier.eissn","2045-2322"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15614"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/73852"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Expression of the methionine sulfoxide reductase lost during evolution extends Drosophila lifespan in a methionine-dependent manner"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e15567"],["dc.bibliographiccitation.journal","eLife"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Schnorrenberg, Sebastian"],["dc.contributor.author","Grotjohann, Tim"],["dc.contributor.author","Vorbrüggen, Gerd"],["dc.contributor.author","Herzig, Alf"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Jakobs, Stefan"],["dc.date.accessioned","2020-12-10T18:48:05Z"],["dc.date.available","2020-12-10T18:48:05Z"],["dc.date.issued","2016"],["dc.description.abstract","Despite remarkable developments in diffraction unlimited super-resolution microscopy, in vivo nanoscopy of tissues and model organisms is still not satisfactorily established and rarely realized. RESOLFT nanoscopy is particularly suited for live cell imaging because it requires relatively low light levels to overcome the diffraction barrier. Previously, we introduced the reversibly switchable fluorescent protein rsEGFP2, which facilitated fast RESOLFT nanoscopy (Grodohann et al., 2012). In that study, as in most other nanoscopy studies, only cultivated single cells were analyzed. Here, we report on the use of rsEGFP2 for live-cell RESOLFT nanoscopy of sub-cellular structures of intact Drosophila melanogaster larvae and of resected tissues. We generated flies expressing fusion proteins of alpha-tubulin and rsEGFP2 highlighting the microtubule cytoskeleton in all cells. By focusing through the intact larval cuticle, we achieved lateral resolution of <60 nm. RESOLFT nanoscopy enabled time-lapse recordings comprising 40 images and facilitated recordings 40 ism deep within fly tissues."],["dc.identifier.doi","10.7554/eLife.15567"],["dc.identifier.eissn","2050-084X"],["dc.identifier.fs","626317"],["dc.identifier.gro","3141660"],["dc.identifier.isi","000379856900001"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13549"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/79008"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","2050-084X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","In vivo super-resolution RESOLFT microscopy of Drosophila melanogaster"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","223"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Genes & Development"],["dc.bibliographiccitation.lastpage","237"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Swan, Laura E."],["dc.contributor.author","Wichmann, C."],["dc.contributor.author","Prange, U."],["dc.contributor.author","Schmid, A."],["dc.contributor.author","Schmidt, M."],["dc.contributor.author","Schwarz, T."],["dc.contributor.author","Ponimaskin, Evgeni G."],["dc.contributor.author","Madeo, F."],["dc.contributor.author","Vorbruggen, G."],["dc.contributor.author","Sigrist, Stephan J."],["dc.date.accessioned","2018-11-07T10:51:47Z"],["dc.date.available","2018-11-07T10:51:47Z"],["dc.date.issued","2004"],["dc.description.abstract","During Drosophila embryogenesis, developing muscles extend growth-cone-like structures to navigate toward specific epidermal attachment sites. Here, we show that the homolog of Glutamate Receptor-Interacting Proteins (DGrip) acts as a key component of proper muscle guidance. Mutations in dgrip impair patterning of ventral longitudinal muscles (VLMs), whereas lateral transverse muscles (LTMs) that attach to intrasegmental attachment sites develop normally. Myoblast fusion, stabilization of muscle contacts, and general muscle function are not impaired in the absence of DGrip. Instead, the proper formation of cellular extensions during guidance fails in dgrip mutant VLMs. DGrip protein concentrates at the ends of VLMs while these muscles guide toward segment border attachment sites. Conversely, LTMs overexpressing DGrip form ectopic cellular extensions that can cause attachment of these muscles to other muscles at segment borders. Our data suggest that DGrip participates in the reception of an attractive signal that emanates from the epidermal attachment sites to direct the motility of developing muscles. This dgrip phenotype should be valuable to study mechanistic principles of Grip function."],["dc.identifier.doi","10.1101/gad.287604"],["dc.identifier.isi","000188716000011"],["dc.identifier.pmid","14729572"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/48962"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cold Spring Harbor Lab Press, Publications Dept"],["dc.relation.issn","0890-9369"],["dc.title","A glutamate receptor-interacting protein homolog organizes muscle guidance in Drosophila"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","225"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Current Biology"],["dc.bibliographiccitation.lastpage","230"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Steigemann, Patrick"],["dc.contributor.author","Molitor, Andreas"],["dc.contributor.author","Fellert, Sonja"],["dc.contributor.author","Jäckle, Herbert"],["dc.contributor.author","Vorbrüggen, Gerd"],["dc.date.accessioned","2021-06-01T10:49:31Z"],["dc.date.available","2021-06-01T10:49:31Z"],["dc.date.issued","2004"],["dc.identifier.doi","10.1016/j.cub.2004.01.006"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/86322"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.issn","0960-9822"],["dc.title","Heparan Sulfate Proteoglycan Syndecan Promotes Axonal and Myotube Guidance by Slit/Robo Signaling"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]