Huet, Antoine Tarquin

[["dc.bibliographiccitation.artnumber","jacs.1c12314"],["dc.bibliographiccitation.journal","Journal of the American Chemical Society"],["dc.contributor.author","Garrido-Charles, Aida"],["dc.contributor.author","Huet, Antoine"],["dc.contributor.author","Matera, Carlo"],["dc.contributor.author","Thirumalai, Anupriya"],["dc.contributor.author","Hernando, Jordi"],["dc.contributor.author","Llebaria, Amadeu"],["dc.contributor.author","Moser, Tobias"],["dc.contributor.author","Gorostiza, Pau"],["dc.date.accessioned","2022-06-01T09:39:05Z"],["dc.date.available","2022-06-01T09:39:05Z"],["dc.date.issued","2022"],["dc.description.abstract","Artificial control of neuronal activity enables the study of neural circuits and restoration of neural functions. Direct, rapid, and sustained photocontrol of intact neurons could overcome the limitations of established electrical stimulation such as poor selectivity. We have developed fast photoswitchable ligands of glutamate receptors (GluRs) to enable neuronal control in the auditory system. The new photoswitchable ligands induced photocurrents in untransfected neurons upon covalently tethering to endogenous GluRs and activating them reversibly with visible light pulses of a few milliseconds. As a proof of concept of these molecular prostheses, we applied them to the ultrafast synapses of auditory neurons of the cochlea that encode sound and provide auditory input to the brain. This drug-based method afforded the optical stimulation of auditory neurons of adult gerbils at hundreds of hertz without genetic manipulation that would be required for their optogenetic control. This indicates that the new photoswitchable ligands are also applicable to the spatiotemporal control of fast spiking interneurons in the brain."],["dc.identifier.doi","10.1021/jacs.1c12314"],["dc.identifier.pmid","35584208"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/108385"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/487"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-572"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","1520-5126"],["dc.relation.issn","0002-7863"],["dc.relation.workinggroup","RG Moser (Molecular Anatomy, Physiology and Pathology of Sound Encoding)"],["dc.relation.workinggroup","RG Huet"],["dc.rights","CC BY-NC-ND 4.0"],["dc.title","Fast Photoswitchable Molecular Prosthetics Control Neuronal Activity in the Cochlea"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e99649"],["dc.bibliographiccitation.issue","24"],["dc.bibliographiccitation.journal","The EMBO Journal"],["dc.bibliographiccitation.volume","37"],["dc.contributor.author","Keppeler, Daniel"],["dc.contributor.author","Merino, Ricardo Martins"],["dc.contributor.author","Lopez de la Morena, David"],["dc.contributor.author","Bali, Burak"],["dc.contributor.author","Huet, Antoine Tarquin"],["dc.contributor.author","Gehrt, Anna"],["dc.contributor.author","Wrobel, Christian"],["dc.contributor.author","Subramanian, Swati"],["dc.contributor.author","Dombrowski, Tobias"],["dc.contributor.author","Wolf, Fred"],["dc.contributor.author","Rankovic, Vladan"],["dc.contributor.author","Neef, Andreas"],["dc.contributor.author","Moser, Tobias"],["dc.date.accessioned","2019-07-09T11:51:47Z"],["dc.date.available","2019-07-09T11:51:47Z"],["dc.date.issued","2018"],["dc.description.abstract","Optogenetic tools, providing non‐invasive control over selected cells, have the potential to revolutionize sensory prostheses for humans. Optogenetic stimulation of spiral ganglion neurons (SGNs) in the ear provides a future alternative to electrical stimulation used in cochlear implants. However, most channelrhodopsins do not support the high temporal fidelity pertinent to auditory coding because they require milliseconds to close after light‐off. Here, we biophysically characterized the fast channelrhodopsin Chronos and revealed a deactivation time constant of less than a millisecond at body temperature. In order to enhance neural expression, we improved its trafficking to the plasma membrane (Chronos‐ES/TS). Following efficient transduction of SGNs using early postnatal injection of the adeno‐associated virus AAV‐PHP.B into the mouse cochlea, fiber‐based optical stimulation elicited optical auditory brainstem responses (oABR) with minimal latencies of 1 ms, thresholds of 5 μJ and 100 μs per pulse, and sizable amplitudes even at 1,000 Hz of stimulation. Recordings from single SGNs demonstrated good temporal precision of light‐evoked spiking. In conclusion, efficient virus‐mediated expression of targeting‐optimized Chronos‐ES/TS achieves ultrafast optogenetic control of neurons."],["dc.identifier.doi","10.15252/embj.201899649"],["dc.identifier.pmid","30396994"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16193"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60011"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","610"],["dc.title","Ultrafast optogenetic stimulation of the auditory pathway by targeting‐optimized Chronos"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","submitted_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Molecular Neuroscience"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Huet, Antoine Tarquin"],["dc.contributor.author","Dombrowski, Tobias"],["dc.contributor.author","Rankovic, Vladan"],["dc.contributor.author","Thirumalai, Anupriya"],["dc.contributor.author","Moser, Tobias"],["dc.date.accessioned","2021-04-14T08:27:59Z"],["dc.date.available","2021-04-14T08:27:59Z"],["dc.date.issued","2021"],["dc.description.abstract","Optogenetic stimulation of type I spiral ganglion neurons (SGNs) promises an alternative to the electrical stimulation by current cochlear implants (CIs) for improved hearing restoration by future optical CIs (oCIs). Most of the efforts in using optogenetic stimulation in the cochlea so far used early postnatal injection of viral vectors carrying blue-light activated channelrhodopsins (ChRs) into the cochlea of mice. However, preparing clinical translation of the oCI requires (i) reliable and safe transduction of mature SGNs of further species and (ii) use of long-wavelength light to avoid phototoxicity. Here, we employed a fast variant of the red-light activated channelrhodopsin Chrimson (f-Chrimson) and different AAV variants to implement optogenetic SGN stimulation in Mongolian gerbils. We compared early postnatal (p8) and adult (\\u0026gt;8 weeks) AAV administration, employing different protocols for injection of AAV-PHP.B and AAV2/6 into the adult cochlea. Success of the optogenetic manipulation was analyzed by optically evoked auditory brainstem response (oABR) and immunohistochemistry of mid-modiolar cryosections of the cochlea. In order to most efficiently evaluate the immunohistochemical results a semi-automatic procedure to identify transduced cells in confocal images was developed. Our results indicate that the rate of SGN transduction is significantly lower for AAV administration into the adult cochlea compared to early postnatal injection. SGN transduction upon AAV administration into the adult cochlea was largely independent of the chosen viral vector and injection approach. The higher the rate of SGN transduction, the lower were oABR thresholds and the larger were oABR amplitudes. Our results highlight the need to optimize viral vectors and virus administration for efficient optogenetic manipulation of SGNs in the adult cochlea for successful clinical translation of SGN-targeting gene therapy and of the oCI."],["dc.identifier.doi","10.3389/fnmol.2021.635897"],["dc.identifier.pmid","33776648"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82465"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/305"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.publisher","Frontiers Media S.A."],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","1662-5099"],["dc.relation.workinggroup","RG Moser (Molecular Anatomy, Physiology and Pathology of Sound Encoding)"],["dc.rights","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Developing Fast, Red-Light Optogenetic Stimulation of Spiral Ganglion Neurons for Future Optical Cochlear Implants"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","EMBO Molecular Medicine"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Bali, Burak"],["dc.contributor.author","Lopez de la Morena, David"],["dc.contributor.author","Mittring, Artur"],["dc.contributor.author","Mager, Thomas"],["dc.contributor.author","Rankovic, Vladan"],["dc.contributor.author","Huet, Antoine Tarquin"],["dc.contributor.author","Moser, Tobias"],["dc.date.accessioned","2021-06-01T09:42:20Z"],["dc.date.available","2021-06-01T09:42:20Z"],["dc.date.issued","2021"],["dc.description.abstract","Abstract Optogenetic stimulation of spiral ganglion neurons (SGNs) in the ear provides a future alternative to electrical stimulation used in current cochlear implants. Here, we employed fast and very fast variants of the red‐light‐activated channelrhodopsin (ChR) Chrimson (f‐Chrimson and vf‐Chrimson) to study their utility for optogenetic stimulation of SGNs in mice. The light requirements were higher for vf‐Chrimson than for f‐Chrimson, even when optimizing membrane expression of vf‐Chrimson by adding potassium channel trafficking sequences. Optogenetic time and intensity coding by single putative SGNs were compared with coding of acoustic clicks. vf‐Chrimson enabled putative SGNs to fire at near‐physiological rates with good temporal precision up to 250 Hz of stimulation. The dynamic range of SGN spike rate coding upon optogenetic stimulation was narrower than for acoustic clicks but larger than reported for electrical stimulation. The dynamic range of spike timing, on the other hand, was more comparable for optogenetic and acoustic stimulation. In conclusion, f‐Chrimson and vf‐Chrimson are promising candidates for optogenetic stimulation of SGNs in auditory research and future cochlear implants."],["dc.description.abstract","Synopsis image Identifying suitable channelrhodopsins is crucial for future optogenetic restoration of sound encoding by optical cochlear implants. Here, fast and very fast light‐activated Chrimsons were compared for their utility to optogenetically encode timing and intensity information in the auditory nerve. Very fast Chrimson increases temporal fidelity but confers lower light sensitivity of optogenetic auditory nerve fiber stimulation compared with fast Chrimson. Adding trafficking sequences of the inwardly rectifying potassium channel 2.1 improved plasma membrane expression of very fast Chrimson enabling shorter stimulus durations The dynamic range, based on the discharge rate, of optogenetic auditory nerve fiber stimulation was narrower than that of acoustic stimulation. The dynamic range, based on temporal precision of spiking, of optogenetic auditory nerve fiber stimulation was broader than that based on discharge rate."],["dc.description.abstract","Identifying suitable channelrhodopsins is crucial for future optogenetic restoration of sound encoding by optical cochlear implants. Here, fast and very fast light‐activated Chrimson were compared for their utility to optogenetically encode timing and intensity information in the auditory nerve. image"],["dc.description.sponsorship","EC | H2020 | H2020 Priority Excellent Science | H2020 European Research Council (ERC)"],["dc.description.sponsorship","Cluster of Excellence; Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells; (MBExC) Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)"],["dc.description.sponsorship","Leibniz Program"],["dc.description.sponsorship","Göttingen Promotionkolleg für Medizinstudierende"],["dc.identifier.doi","10.15252/emmm.202013391"],["dc.identifier.pmid","33960685"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85221"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/255"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","1757-4684"],["dc.relation.issn","1757-4676"],["dc.relation.workinggroup","RG Mager (Advanced Optogenes)"],["dc.relation.workinggroup","RG Moser (Molecular Anatomy, Physiology and Pathology of Sound Encoding)"],["dc.rights","This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited."],["dc.title","Utility of red‐light ultrafast optogenetic stimulation of the auditory pathway"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","eaao0540"],["dc.bibliographiccitation.issue","449"],["dc.bibliographiccitation.journal","Science Translational Medicine"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Wrobel, Christian"],["dc.contributor.author","Dieter, Alexander"],["dc.contributor.author","Huet, Antoine"],["dc.contributor.author","Keppeler, Daniel"],["dc.contributor.author","Duque-Afonso, Carlos J."],["dc.contributor.author","Vogl, Christian"],["dc.contributor.author","Hoch, Gerhard"],["dc.contributor.author","Jeschke, Marcus"],["dc.contributor.author","Moser, Tobias"],["dc.date.accessioned","2020-12-10T18:36:47Z"],["dc.date.available","2020-12-10T18:36:47Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1126/scitranslmed.aao0540"],["dc.identifier.eissn","1946-6242"],["dc.identifier.issn","1946-6234"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/76736"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Optogenetic stimulation of cochlear neurons activates the auditory pathway and restores auditory-driven behavior in deaf adult gerbils"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2253"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","The Journal of Neuroscience"],["dc.bibliographiccitation.lastpage","2267"],["dc.bibliographiccitation.volume","42"],["dc.contributor.author","Huet, Antoine"],["dc.contributor.author","Batrel, Charlène"],["dc.contributor.author","Dubernard, Xavier"],["dc.contributor.author","Kleiber, Jean-Charles"],["dc.contributor.author","Desmadryl, Gilles"],["dc.contributor.author","Venail, Frédéric"],["dc.contributor.author","Liberman, M. Charles"],["dc.contributor.author","Nouvian, Régis"],["dc.contributor.author","Puel, Jean-Luc"],["dc.contributor.author","Bourien, Jérôme"],["dc.date.accessioned","2022-05-02T08:09:30Z"],["dc.date.available","2022-05-02T08:09:30Z"],["dc.date.issued","2022"],["dc.identifier.doi","10.1523/JNEUROSCI.0858-21.2022"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/107394"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-561"],["dc.relation.eissn","1529-2401"],["dc.relation.issn","0270-6474"],["dc.title","Peristimulus Time Responses Predict Adaptation and Spontaneous Firing of Auditory-Nerve Fibers: From Rodents Data to Humans"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","1750"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Mager, Thomas"],["dc.contributor.author","Lopez de la Morena, David"],["dc.contributor.author","Senn, Verena"],["dc.contributor.author","Schlotte, Johannes"],["dc.contributor.author","D´Errico, Anna"],["dc.contributor.author","Feldbauer, Katrin"],["dc.contributor.author","Wrobel, Christian"],["dc.contributor.author","Jung, Sangyong"],["dc.contributor.author","Bodensiek, Kai"],["dc.contributor.author","Rankovic, Vladan"],["dc.contributor.author","Browne, Lorcan"],["dc.contributor.author","Huet, Antoine"],["dc.contributor.author","Jüttner, Josephine"],["dc.contributor.author","Wood, Phillip G"],["dc.contributor.author","Letzkus, Johannes J."],["dc.contributor.author","Moser, Tobias"],["dc.contributor.author","Bamberg, Ernst"],["dc.date.accessioned","2018-07-12T06:58:57Z"],["dc.date.available","2018-07-12T06:58:57Z"],["dc.date.issued","2018"],["dc.description.abstract","Optogenetics revolutionizes basic research in neuroscience and cell biology and bears potential for medical applications. We develop mutants leading to a unifying concept for the construction of various channelrhodopsins with fast closing kinetics. Due to different absorption maxima these channelrhodopsins allow fast neural photoactivation over the whole range of the visible spectrum. We focus our functional analysis on the fast-switching, red light-activated Chrimson variants, because red light has lower light scattering and marginal phototoxicity in tissues. We show paradigmatically for neurons of the cerebral cortex and the auditory nerve that the fast Chrimson mutants enable neural stimulation with firing frequencies of several hundred Hz. They drive spiking at high rates and temporal fidelity with low thresholds for stimulus intensity and duration. Optical cochlear implants restore auditory nerve activity in deaf mice. This demonstrates that the mutants facilitate neuroscience research and future medical applications such as hearing restoration."],["dc.identifier.doi","10.1038/s41467-018-04146-3"],["dc.identifier.pmid","29717130"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15593"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/15178"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation.eissn","2041-1723"],["dc.relation.eissn","2041-1723"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","High frequency neural spiking and auditory signaling by ultrafast red-shifted optogenetics"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]