Ninković, Milena

[["dc.bibliographiccitation.firstpage","1932"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Analytical Chemistry"],["dc.bibliographiccitation.lastpage","1940"],["dc.bibliographiccitation.volume","94"],["dc.contributor.author","Dučić, Tanja"],["dc.contributor.author","Ninkovic, Milena"],["dc.contributor.author","Martínez-Rovira, Immaculada"],["dc.contributor.author","Sperling, Swetlana"],["dc.contributor.author","Rohde, Veit"],["dc.contributor.author","Dimitrijević, Dragoljub"],["dc.contributor.author","Jover Mañas, Gabriel Vicent"],["dc.contributor.author","Vaccari, Lisa"],["dc.contributor.author","Birarda, Giovanni"],["dc.contributor.author","Yousef, Ibraheem"],["dc.date.accessioned","2022-02-01T10:32:13Z"],["dc.date.available","2022-02-01T10:32:13Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1021/acs.analchem.1c02076"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/99037"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-517"],["dc.relation.eissn","1520-6882"],["dc.relation.issn","0003-2700"],["dc.title","Live-Cell Synchrotron-Based FTIR Evaluation of Metabolic Compounds in Brain Glioblastoma Cell Lines after Riluzole Treatment"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","201"],["dc.bibliographiccitation.journal","Frontiers in physiology"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Herrmann, Solveig"],["dc.contributor.author","Ninkovic, Milena"],["dc.contributor.author","Kohl, Tobias"],["dc.contributor.author","Pardo, Luis A."],["dc.date.accessioned","2019-07-09T11:40:08Z"],["dc.date.available","2019-07-09T11:40:08Z"],["dc.date.issued","2013"],["dc.description.abstract","Although crucial for their correct function, the mechanisms controlling surface expression of ion channels are poorly understood. In the case of the voltage-gated potassium channel KV10.1, this is determinant not only for its physiological function in brain, but also for its pathophysiology in tumors and possible use as a therapeutic target. The Golgi resident protein PIST binds several membrane proteins, thereby modulating their expression. Here we describe a PDZ domain-mediated interaction of KV10.1 and PIST, which enhances surface levels of KV10.1. The functional, but not the physical interaction of both proteins is dependent on the coiled-coil and PDZ domains of PIST; insertion of eight amino acids in the coiled-coil domain to render the neural form of PIST (nPIST) and the corresponding short isoform in an as-of-yet unknown form abolishes the effect. In addition, two new isoforms of PIST (sPIST and nsPIST) lacking nearly the complete PDZ domain were cloned and shown to be ubiquitously expressed. PIST and KV10.1 co-precipitate from native and expression systems. nPIST also showed interaction, but did not alter the functional expression of the channel. We could not document physical interaction between KV10.1 and sPIST, but it reduced KV10.1 functional expression in a dominant-negative manner. nsPIST showed weak physical interaction and no functional effect on KV10.1. We propose these isoforms to work as modulators of PIST function via regulating the binding on interaction partners."],["dc.identifier.doi","10.3389/fphys.2013.00201"],["dc.identifier.fs","604131"],["dc.identifier.pmid","23966943"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10690"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58100"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1664-042X"],["dc.rights","CC BY 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/3.0"],["dc.title","PIST (GOPC) modulates the oncogenic voltage-gated potassium channel KV10.1."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Translational Stroke Research"],["dc.contributor.author","Döring, Katja"],["dc.contributor.author","Schroeder, Henning"],["dc.contributor.author","Fischer, André"],["dc.contributor.author","Sperling, Swetlana"],["dc.contributor.author","Ninkovic, Milena"],["dc.contributor.author","Stadelmann, Christine"],["dc.contributor.author","Mielke, Dorothee"],["dc.contributor.author","Rohde, Veit"],["dc.contributor.author","Malinova, Vesna"],["dc.date.accessioned","2022-02-01T10:31:59Z"],["dc.date.available","2022-02-01T10:31:59Z"],["dc.date.issued","2022"],["dc.description.abstract","Abstract Cerebral vasospasm is a highly investigated phenomenon in neurovascular research. Experimental vasospasm models are irreplaceable for the evaluation of new antivasospastic drugs. In this study, we assessed the reliability of in vivo vasospasm induction by ultrasound application in the chicken chorioallantoic membrane (CAM) model. After incubation of fertilized chicken eggs for four days, a fenestration was performed to enable examination of the CAM vessels. On the thirteenth day, continuous-wave ultrasound (3 MHz, 1 W/cm 2 ) was applied on the CAM vessels for 60 s. The ultrasound effect on the vessels was recorded by life imaging (5-MP HD-microscope camera, Leica®). The induced vessel diameter changes were evaluated in a defined time interval of 20 min using a Fiji macro. The vessel diameter before and after sonication was measured and the relative diameter reduction was determined. A first reduction of vessel diameter was observed after three minutes with an average vessel-diameter decrease to 77%. The maximum reduction in vessel diameter was reached eight minutes after sonication with an average vessel diameter decrease to 57% (mean relative diameter reduction of 43%, range 44–61%), ANOVA, p  = 0.0002. The vasospasm persisted for all 20 recorded minutes post induction. Vasospasm can be reliably induced by short application of 3 MHz-ultrasound to the CAM vessels. This might be a suitable in vivo model for the evaluation of drug effects on vasospasm in an experimental setting as intermediary in the transition process from in vitro to in vivo assessment using animal models."],["dc.description.abstract","Abstract Cerebral vasospasm is a highly investigated phenomenon in neurovascular research. Experimental vasospasm models are irreplaceable for the evaluation of new antivasospastic drugs. In this study, we assessed the reliability of in vivo vasospasm induction by ultrasound application in the chicken chorioallantoic membrane (CAM) model. After incubation of fertilized chicken eggs for four days, a fenestration was performed to enable examination of the CAM vessels. On the thirteenth day, continuous-wave ultrasound (3 MHz, 1 W/cm 2 ) was applied on the CAM vessels for 60 s. The ultrasound effect on the vessels was recorded by life imaging (5-MP HD-microscope camera, Leica®). The induced vessel diameter changes were evaluated in a defined time interval of 20 min using a Fiji macro. The vessel diameter before and after sonication was measured and the relative diameter reduction was determined. A first reduction of vessel diameter was observed after three minutes with an average vessel-diameter decrease to 77%. The maximum reduction in vessel diameter was reached eight minutes after sonication with an average vessel diameter decrease to 57% (mean relative diameter reduction of 43%, range 44–61%), ANOVA, p  = 0.0002. The vasospasm persisted for all 20 recorded minutes post induction. Vasospasm can be reliably induced by short application of 3 MHz-ultrasound to the CAM vessels. This might be a suitable in vivo model for the evaluation of drug effects on vasospasm in an experimental setting as intermediary in the transition process from in vitro to in vivo assessment using animal models."],["dc.identifier.doi","10.1007/s12975-021-00960-y"],["dc.identifier.pii","960"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/98997"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-517"],["dc.relation.eissn","1868-601X"],["dc.relation.issn","1868-4483"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","In Vivo Vasospasm Induction by Ultrasound Application in the Chicken Chorioallantoic Membrane Model"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1037"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Acta Neurochirurgica"],["dc.bibliographiccitation.lastpage","1045"],["dc.bibliographiccitation.volume","161"],["dc.contributor.author","Fiss, Ingo"],["dc.contributor.author","Hussein, Abdelhalim"],["dc.contributor.author","Barrantes-Freer, Alonso"],["dc.contributor.author","Sperling, Swetlana"],["dc.contributor.author","Hernandez-Duran, Silvia"],["dc.contributor.author","Wolfert, Christina"],["dc.contributor.author","Pukrop, Tobias"],["dc.contributor.author","Ninkovic, Milena"],["dc.contributor.author","Bleckmann, Annalen"],["dc.contributor.author","Rohde, Veit"],["dc.contributor.author","Mielke, Dorothee"],["dc.contributor.author","Schatlo, Bawarjan"],["dc.date.accessioned","2020-12-10T14:10:53Z"],["dc.date.available","2020-12-10T14:10:53Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1007/s00701-019-03842-3"],["dc.identifier.eissn","0942-0940"],["dc.identifier.issn","0001-6268"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/70910"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Cerebral metastases: do size, peritumoral edema, or multiplicity predict infiltration into brain parenchyma?"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","207"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Anticancer Research"],["dc.bibliographiccitation.lastpage","214"],["dc.bibliographiccitation.volume","39"],["dc.contributor.author","SACHKOVA, ALEKSANDRA"],["dc.contributor.author","SPERLING, SWETLANA"],["dc.contributor.author","MIELKE, DOROTHEE"],["dc.contributor.author","SCHATLO, BAWARJAN"],["dc.contributor.author","ROHDE, VEIT"],["dc.contributor.author","NINKOVIC, MILENA"],["dc.date.accessioned","2020-12-10T18:43:04Z"],["dc.date.available","2020-12-10T18:43:04Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.21873/anticanres.13099"],["dc.identifier.eissn","1791-7530"],["dc.identifier.issn","0250-7005"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78182"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Combined Applications of Repurposed Drugs and Their Detrimental Effects on Glioblastoma Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","228"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Analytical Biochemistry"],["dc.bibliographiccitation.lastpage","233"],["dc.bibliographiccitation.volume","292"],["dc.contributor.author","Ninkovic, Milena"],["dc.contributor.author","Riester, Daniel"],["dc.contributor.author","Wirsching, Frank"],["dc.contributor.author","Dietrich, Rüdiger"],["dc.contributor.author","Schwienhorst, Andreas"],["dc.date.accessioned","2021-06-01T10:47:25Z"],["dc.date.available","2021-06-01T10:47:25Z"],["dc.date.issued","2001"],["dc.identifier.doi","10.1006/abio.2001.5078"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85596"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.issn","0003-2697"],["dc.title","Fluorogenic Assay for Penicillin G Acylase Activity"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","S1773224721005141"],["dc.bibliographiccitation.firstpage","102834"],["dc.bibliographiccitation.journal","Journal of Drug Delivery Science and Technology"],["dc.bibliographiccitation.volume","66"],["dc.contributor.author","Döring, Katja"],["dc.contributor.author","Sperling, Swetlana"],["dc.contributor.author","Ninkovic, Milena"],["dc.contributor.author","Gasimov, Turab"],["dc.contributor.author","Stadelmann, Christine"],["dc.contributor.author","Streit, Frank"],["dc.contributor.author","Binder, Lutz"],["dc.contributor.author","Rohde, Veit"],["dc.contributor.author","Malinova, Vesna"],["dc.date.accessioned","2021-12-01T09:23:36Z"],["dc.date.available","2021-12-01T09:23:36Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1016/j.jddst.2021.102834"],["dc.identifier.pii","S1773224721005141"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/94703"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-478"],["dc.relation.issn","1773-2247"],["dc.title","Ultrasound-induced release of nimodipine from drug-loaded block copolymers: In vitro analysis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","118"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","European Journal of Applied Physiology"],["dc.bibliographiccitation.lastpage","130"],["dc.bibliographiccitation.volume","124"],["dc.contributor.author","Saul, D."],["dc.contributor.author","Ninkovic, M."],["dc.contributor.author","Komrakova, M."],["dc.contributor.author","Wolff, P."],["dc.contributor.author","Simka, P."],["dc.contributor.author","Gasimov, T."],["dc.contributor.author","Menger, B."],["dc.contributor.author","Hoffmann, D. B."],["dc.contributor.author","Rohde, V."],["dc.contributor.author","Sehmisch, S."],["dc.date.accessioned","2020-06-15T06:51:43Z"],["dc.date.available","2020-06-15T06:51:43Z"],["dc.date.issued","2017"],["dc.description.abstract","Estrogen deficiency and aging are associated with osteoporosis, impaired bone healing, and lower cognitive performance. Close functional and physical connections occur between bone and the central nervous system. An anti-inflammatory drug, zileuton (which is an inhibitor of arachidonate 5-lipoxygenase), is known to have a positive effect on bone tissue repair and brain ischemia. We studied the effect of zileuton on osteopenic bone and its healing and on the genes considered to be crucial for the cross talks between bone and brain. Three-month-old Sprague-Dawley rats were ovariectomized or left untreated. After 8 wk, bilateral metaphyseal tibia osteotomy with plate osteosynthesis was performed in all rats. Ovariectomized rats were fed with food containing zileuton (1, 10, or 100 mg/kg body wt) for 5 wk. In tibiae, bone volume, callus and cortical volume, and gene expression of osteocalcin and alkaline phosphatase were enhanced by zileuton (10 or 100 mg); biomechanical properties and bone density were not changed. In femur, zileuton enlarged cortical volume distal and trabecular volume proximal, decreasing their density. The expression level of brain Sema3a, known to regulate bone mass positively, was downregulated after ovariectomy. In contrast, bone Sema4d, a negative regulator of bone mass, was upregulated in the tibia callus after ovariectomy, whereas zileuton treatment (10 or 100 mg) resulted in reverse effects. Here, we describe for the first time the expression of Rbbp4 mRNA and its increase in tibia after ovariectomy. Zileuton caused downregulation of Rbbp4 in the hippocampus and had an effect on bone healing, changed the expression of genes involved in cross talk between bones and brain, and may be a potent drug for further examination in estrogen deficiency-related dysfunction(s)."],["dc.identifier.doi","10.1152/japplphysiol.01126.2016"],["dc.identifier.pmid","28860177"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/66241"],["dc.language.iso","en"],["dc.relation.eissn","1522-1601"],["dc.relation.issn","8750-7587"],["dc.title","Effect of zileuton on osteoporotic bone and its healing, expression of bone and brain genes in rats"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","96697"],["dc.bibliographiccitation.firstpage","96697"],["dc.bibliographiccitation.issue","57"],["dc.bibliographiccitation.journal","Oncotarget"],["dc.bibliographiccitation.lastpage","96709"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Sperling, Swetlana"],["dc.contributor.author","Aung, Thiha"],["dc.contributor.author","Martin, Sabine"],["dc.contributor.author","Rohde, Veit"],["dc.contributor.author","Ninkovic, Milena"],["dc.date.accessioned","2018-10-09T07:07:52Z"],["dc.date.available","2018-10-09T07:07:52Z"],["dc.date.issued","2017-11-14"],["dc.description.abstract","A small subpopulation of tumor stem-like cells has the capacity to initiate tumors and mediate radio- and chemoresistance in diverse cancers hence also in glioblastoma (GBM). It has been reported that this capacity of tumor initiation in the brain is mainly dependent on the body's nutrient supply. This population of so-called brain tumor initiating or brain tumor stem-like cells (BTSCs) is able to extract nutrients like glucose with a higher affinity. Riluzole, a drug approved for treating amyotrophic lateral sclerosis (ALS), was reported to possess anticancer properties, affecting the glutamate metabolism. We report that riluzole treatment inhibits the growth of brain tumor stem-like cells enriched cultures isolated from two human glioblastomas. The effects of riluzole on these cells were associated with an inhibition of a poor prognostic indicator: glucose transporter 3 (GLUT3). A decrease in GLUT3 is associated with a decrease in the p-Akt/HIF1α pathway. Further, downregulation of the DNA (Cytosine-5-)-methyltransferase 1 (DNMT1) gene that causes hypermethylation of various tumor-suppressor genes and leads to a poor prognosis in GBM, was detected. Two hallmarks of cancer cells-proliferation and cell death-were positively influenced by riluzole treatment. Finally, we observed that riluzole reduced the tumor growth in in vivo CAM assay, suggesting it could be a possible synergistic drug for the treatment of glioblastoma."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2017"],["dc.fs.pkfprnr","86427"],["dc.identifier.doi","10.18632/oncotarget.18043"],["dc.identifier.fs","633522"],["dc.identifier.pmid","29228563"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14509"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/15891"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation.eissn","1949-2553"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Riluzole: a potential therapeutic intervention in human brain tumor stem-like cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2328"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Journal of Bacteriology"],["dc.bibliographiccitation.lastpage","2339"],["dc.bibliographiccitation.volume","186"],["dc.contributor.author","Hildmann, Christian"],["dc.contributor.author","Ninkovic, Milena"],["dc.contributor.author","Dietrich, Rüdiger"],["dc.contributor.author","Wegener, Dennis"],["dc.contributor.author","Riester, Daniel"],["dc.contributor.author","Zimmermann, Thomas"],["dc.contributor.author","Birch, Olwen M."],["dc.contributor.author","Bernegger, Christine"],["dc.contributor.author","Loidl, Peter"],["dc.contributor.author","Schwienhorst, Andreas"],["dc.date.accessioned","2021-06-01T10:47:34Z"],["dc.date.available","2021-06-01T10:47:34Z"],["dc.date.issued","2004"],["dc.description.abstract","ABSTRACT The full-length gene encoding the histone deacetylase (HDAC)-like amidohydrolase (HDAH) from Bordetella or Alcaligenes ( Bordetella/Alcaligenes ) strain FB188 (DSM 11172) was cloned using degenerate primer PCR combined with inverse-PCR techniques and ultimately expressed in Escherichia coli . The expressed enzyme was biochemically characterized and found to be similar to the native enzyme for all properties examined. Nucleotide sequence analysis revealed an open reading frame of 1,110 bp which encodes a polypeptide with a theoretical molecular mass of 39 kDa. Interestingly, peptide sequencing disclosed that the N-terminal methionine is lacking in the mature wild-type enzyme, presumably due to the action of methionyl aminopeptidase. Sequence database searches suggest that the new amidohydrolase belongs to the HDAC superfamily, with the closest homologs being found in the subfamily assigned acetylpolyamine amidohydrolases (APAH). The APAH subfamily comprises enzymes or putative enzymes from such diverse microorganisms as Pseudomonas aeruginosa , Archaeoglobus fulgidus , and the actinomycete Mycoplana ramosa (formerly M. bullata ). The FB188 HDAH, however, is only moderately active in catalyzing the deacetylation of acetylpolyamines. In fact, FB188 HDAH exhibits significant activity in standard HDAC assays and is inhibited by known HDAC inhibitors such as trichostatin A and suberoylanilide hydroxamic acid (SAHA). Several lines of evidence indicate that the FB188 HDAH is very similar to class 1 and 2 HDACs and contains a Zn 2+ ion in the active site which contributes significantly to catalytic activity. Initial biotechnological applications demonstrated the extensive substrate spectrum and broad optimum pH range to be excellent criteria for using the new HDAH from Bordetella/Alcaligenes strain FB188 as a biocatalyst in technical biotransformations, e.g., within the scope of human immunodeficiency virus reverse transcriptase inhibitor synthesis."],["dc.identifier.doi","10.1128/JB.186.8.2328-2339.2004"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85648"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","1098-5530"],["dc.relation.issn","0021-9193"],["dc.title","A New Amidohydrolase from Bordetella or Alcaligenes Strain FB188 with Similarities to Histone Deacetylases"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]