Pech, Ulrike

[["dc.bibliographiccitation.firstpage","967"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","The Journal of Heart and Lung Transplantation"],["dc.bibliographiccitation.lastpage","978"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Fehrenbach, A."],["dc.contributor.author","Pufe, T."],["dc.contributor.author","Wittwer, Thorsten"],["dc.contributor.author","Nagib, R."],["dc.contributor.author","Dreyer, N."],["dc.contributor.author","Pech, T."],["dc.contributor.author","Petersen, W."],["dc.contributor.author","Fehrenbach, H."],["dc.contributor.author","Wahlers, T."],["dc.contributor.author","Richter, J."],["dc.date.accessioned","2018-11-07T10:36:37Z"],["dc.date.available","2018-11-07T10:36:37Z"],["dc.date.issued","2003"],["dc.description.abstract","Background: After clinical lung transplantation, the amount of vascular endothelial growth factor (VEGF) was found to be decreased in the bronchoalveolar lavage from lungs with acute lung injury. Since Type II pneumocytes are a major site of VEGF synthesis, VEGF depression may be an indicator of pulmonary epithelial damage after ischemia and reperfusion. Methods: Using an established rat lung model, we investigated the relationship between VEGF protein expression, oxygenation capacity and structural integrity after extracorporeal ischemia and reperfusion (ischemia 6 hours at 10degreesC, reperfusion 50 minutes) and preservation with either low-potassium dextran solution (Perfadex 40 kD, n = 8) or Celsior (n = 6). Untreated, non-ischemic lungs served as controls (n = 5 per group). Perfusate oxygenation was recorded during reperfusion. An enzyme-linked immunoassay (ELISA) for VEGF protein and reverse transcription-polymerase chain reaction (RT-PCR) for mRNA splice variants were determined on tissue collected from the left lungs, whereas the right lungs were fixed by vascular perfusion for VEGF immunohistochemistry as well as structural analysis by light and electron microscopy. Tissue collection by systematic uniform random sampling was representative for the whole organ and allowed for quantification of structures by stereological means. Results: After ischemia and reperfusion, the 3 major VEGF isoforms, VEGF(120), VEGF(164) and VEGF(188), were present. VEGF protein expression was reduced, which correlated significantly with perfusate oxygenation (r = 0.736; p = 0.002) at the end of reperfusion. It was inversely related to Type 11 cell volume (r = 0.600; p = 0.047). VEGF protein was localized by immunohistochemistry in Type 11 pneumocytes, alveolar macrophages as well as bronchial epithelium, and staining intensity of Type II cells was reduced after ischemia and reperfusion. Alveolar edema did not occur but significant interstitial edema accumulated around vessels and in the blood-gas barrier, which showed a higher degree of epithelial damage after preservation with Celsior compared with the other groups. Conclusions: Depression in VEGF protein expression can be considered an indicator for increased alveolar epithelial damage. Preservation with low-potassium, dextran solution resulted in improved oxygenation and tissue integrity compared with Celsior."],["dc.identifier.doi","10.1016/S1053-2498(02)01157-9"],["dc.identifier.isi","000185157100004"],["dc.identifier.pmid","12957606"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/45371"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Inc"],["dc.relation.issn","1053-2498"],["dc.title","Reduced vascular endothelial growth factor correlates with alveolar epithelial damage after experimental ischemia and reperfusion"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1169"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Biochimica et Biophysica Acta (BBA) - General Subjects"],["dc.bibliographiccitation.lastpage","1178"],["dc.bibliographiccitation.volume","1820"],["dc.contributor.author","Riemensperger, Thomas"],["dc.contributor.author","Pech, Ulrike"],["dc.contributor.author","Dipt, Shubham"],["dc.contributor.author","Fiala, Andre"],["dc.date.accessioned","2018-11-07T09:08:06Z"],["dc.date.available","2018-11-07T09:08:06Z"],["dc.date.issued","2012"],["dc.description.abstract","Background: Drosophila melanogaster is one of the best-studied model organisms in biology, mainly because of the versatility of methods by which heredity and specific expression of genes can be traced and manipulated. Sophisticated genetic tools have been developed to express transgenes in selected cell types, and these techniques can be utilized to target DNA-encoded fluorescence probes to genetically defined subsets of neurons. Neuroscientists make use of this approach to monitor the activity of restricted types or subsets of neurons in the brain and the peripheral nervous system. Since membrane depolarization is typically accompanied by an increase in intracellular calcium ions, calcium-sensitive fluorescence proteins provide favorable tools to monitor the spatio-temporal activity across groups of neurons. Scope of review: Here we describe approaches to perform optical calcium imaging in Drosophila in consideration of various calcium sensors and expression systems. In addition, we outline by way of examples for which particular neuronal systems in Drosophila optical calcium imaging have been used. Finally, we exemplify briefly how optical calcium imaging in the brain of Drosophila can be carried out in practice. Major conclusions and general significance: Drosophila provides an excellent model organism to combine genetic expression systems with optical calcium imaging in order to investigate principles of sensory coding, neuronal plasticity, and processing of neuronal information underlying behavior. This article is part of a Special Issue entitled Biochemical, Biophysical and Genetic Approaches to Intracellular Calcium Signaling. (C) 2012 Elsevier B.V. All rights reserved."],["dc.identifier.doi","10.1016/j.bbagen.2012.02.013"],["dc.identifier.isi","000305595300003"],["dc.identifier.pmid","22402253"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/25948"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","0304-4165"],["dc.title","Optical calcium imaging in the nervous system of Drosophila melanogaster"],["dc.type","review"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3992"],["dc.bibliographiccitation.issue","17"],["dc.bibliographiccitation.journal","The Journal of Comparative Neurology"],["dc.bibliographiccitation.lastpage","4026"],["dc.bibliographiccitation.volume","521"],["dc.contributor.author","Pech, Ulrike"],["dc.contributor.author","Pooryasin, Atefeh"],["dc.contributor.author","Birman, Serge"],["dc.contributor.author","Fiala, Andre"],["dc.date.accessioned","2018-11-07T09:17:25Z"],["dc.date.available","2018-11-07T09:17:25Z"],["dc.date.issued","2013"],["dc.description.abstract","The mushroom body of the insect brain represents a neuronal circuit involved in the control of adaptive behavior, e.g., associative learning. Its function relies on the modulation of Kenyon cell activity or synaptic transmitter release by biogenic amines, e.g., octopamine, dopamine, or serotonin. Therefore, for a comprehensive understanding of the mushroom body, it is of interest not only to determine which modulatory neurons interact with Kenyon cells but also to pinpoint where exactly in the mushroom body they do so. To accomplish the latter, we made use of the GRASP technique and created transgenic Drosophila melanogaster that carry one part of a membrane-bound splitGFP in Kenyon cells, along with a cytosolic red fluorescent marker. The second part of the splitGFP is expressed in distinct neuronal populations using cell-specific Gal4 drivers. GFP is reconstituted only if these neurons interact with Kenyon cells in close proximity, which, in combination with two-photon microscopy, provides a very high spatial resolution. We characterize spatially and microstructurally distinct contact regions between Kenyon cells and dopaminergic, serotonergic, and octopaminergic/tyraminergic neurons in all subdivisions of the mushroom body. Subpopulations of dopaminergic neurons contact complementary lobe regions densely. Octopaminergic/tyraminergic neurons contact Kenyon cells sparsely and are restricted mainly to the calyx, the -lobes, and the -lobes. Contacts of Kenyon cells with serotonergic neurons are heterogeneously distributed over the entire mushroom body. In summary, the technique enables us to localize precisely a segmentation of the mushroom body by differential contacts with aminergic neurons. J. Comp. Neurol. 521:3992-4026, 2013. (c) 2013 Wiley Periodicals, Inc."],["dc.identifier.doi","10.1002/cne.23388"],["dc.identifier.isi","000325461300008"],["dc.identifier.pmid","23784863"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28161"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","0021-9967"],["dc.title","Localization of the Contacts Between Kenyon Cells and Aminergic Neurons in the Drosophila melanogaster Brain Using SplitGFP Reconstitution"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1208"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Journal of Thoracic and Cardiovascular Surgery"],["dc.bibliographiccitation.lastpage","1216"],["dc.bibliographiccitation.volume","125"],["dc.contributor.author","Wittwer, Thorsten"],["dc.contributor.author","Albes, J. M."],["dc.contributor.author","Fehrenbach, A."],["dc.contributor.author","Pech, T."],["dc.contributor.author","Franke, Ulrich F. W."],["dc.contributor.author","Richter, J."],["dc.contributor.author","Wahlers, T."],["dc.date.accessioned","2018-11-07T10:38:42Z"],["dc.date.available","2018-11-07T10:38:42Z"],["dc.date.issued","2003"],["dc.description.abstract","Objective: Optimal preservation of postischemic graft function is essential in lung transplantation. Antegrade flush perfusion with modified Euro-Collins solution represents the standard technique worldwide. However, growing evidence suggests the superiority of extracellular-type Perfadex solution (Vitrolife AB, Gothenburg, Germany) over Euro-Collins solution. During ischemia and reperfusion, endogenous pulmonary nitric oxide synthesis is decreased, and therefore therapeutic stimulation of the nitric oxide pathway might be beneficial in ameliorating ischemia-reperfusion damage. However, research mainly focuses on nitric oxide supplementation of intracellular solutions, and no studies exist in which the effect of nitroglycerin on Perfadex preservation quality is evaluated. Methods: Eight rat lungs each were preserved with Perfadex solution with or without nitroglycerin (0.1 mg/mL) and compared with low-potassium Euro-Collins solution. Postischemic lungs were reventilated and reperfused, and oxygenation capacity, pulmonary vascular resistance, and peak inspiratory pressures were monitored continuously. Stereological analysis was used for evaluation of pulmonary edema and assessment of the vasculature. Statistics were performed by using different analysis of variance models. Results: The oxygenation capacity of the Perfadex-preserved groups was higher compared with that of the low-potassium Euro-Collins solution group (P <.03). By using nitroglycerin, flush-perfusion time was reduced, and Perfadex solution with nitroglycerin-protected lungs showed superior oxygenation capacity compared with that seen in Perfadex solution-protected organs (P <.01). Furthermore, pulmonary vascular resistance and peak inspiratory pressures were improved in the nitroglycerin group (P <.01). Stereology revealed comparable intrapulmonary edema between groups and a trend toward less vasoconstricted vasculature in Perfadex with nitroglycerin-protected lungs. Conclusions: Perfadex solution provides superior lung preservation in terms of postischemic oxygenation capacity than Euro-Collins solution. Supplementation of the nitric oxide pathway by nitroglycerin further enhances functional outcome of Perfadex-preserved organs and might be an easily applicable tool in clinical lung transplantation."],["dc.identifier.doi","10.1016/S0022-5223(02)73244-3"],["dc.identifier.isi","000183864700005"],["dc.identifier.pmid","12830037"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/45872"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Mosby, Inc"],["dc.publisher.place","St louis"],["dc.relation.conference","82nd Annual Meeting of the American-Association-for-Thoracic-Surgery"],["dc.relation.eventlocation","WASHINGTON, D.C."],["dc.relation.issn","0022-5223"],["dc.title","Experimental lung preservation with Perfadex: Effect of the NO-donor nitroglycerin on postischemic outcome"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1819"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Journal of Neuroscience"],["dc.bibliographiccitation.lastpage","1837"],["dc.bibliographiccitation.volume","34"],["dc.contributor.author","Barth, Jonas"],["dc.contributor.author","Dipt, Shubham"],["dc.contributor.author","Pech, Ulrike"],["dc.contributor.author","Hermann, Moritz"],["dc.contributor.author","Riemensperger, Thomas"],["dc.contributor.author","Fiala, Andre"],["dc.date.accessioned","2018-11-07T09:44:54Z"],["dc.date.available","2018-11-07T09:44:54Z"],["dc.date.issued","2014"],["dc.description.abstract","Training can improve the ability to discriminate between similar, confusable stimuli, including odors. One possibility of enhancing behaviorally expressed discrimination (i.e., sensory acuity) relies on differential associative learning, during which animals are forced to detect the differences between similar stimuli. Drosophila represents a key model organism for analyzing neuronal mechanisms underlying both odor processing and olfactory learning. However, the ability of flies to enhance fine discrimination between similar odors through differential associative learning has not been analyzed in detail. We performed associative conditioning experiments using chemically similar odorants that we show to evoke overlapping neuronal activity in the fly's antennal lobes and highly correlated activity in mushroom body lobes. We compared the animals' performance in discriminating between these odors after subjecting them to one of two types of training: either absolute conditioning, in which only one odor is reinforced, or differential conditioning, in which one odor is reinforced and a second odor is explicitly not reinforced. First, we show that differential conditioning decreases behavioral generalization of similar odorants in a choice situation. Second, we demonstrate that this learned enhancement in olfactory acuity relies on both conditioned excitation and conditioned inhibition. Third, inhibitory local interneurons in the antennal lobes are shown to be required for behavioral fine discrimination between the two similar odors. Fourth, differential, but not absolute, training causes decorrelation of odor representations in the mushroom body. In conclusion, differential training with similar odors ultimately induces a behaviorally expressed contrast enhancement between the two similar stimuli that facilitates fine discrimination."],["dc.identifier.doi","10.1523/JNEUROSCI.2598-13.2014"],["dc.identifier.isi","000331455000024"],["dc.identifier.pmid","24478363"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/34499"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Soc Neuroscience"],["dc.relation.issn","0270-6474"],["dc.title","Differential Associative Training Enhances Olfactory Acuity in Drosophila melanogaster"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Chemical Senses"],["dc.bibliographiccitation.volume","41"],["dc.contributor.author","Fiala, Andre"],["dc.contributor.author","Pech, Ulrike"],["dc.date.accessioned","2018-11-07T10:14:57Z"],["dc.date.available","2018-11-07T10:14:57Z"],["dc.date.issued","2016"],["dc.format.extent","388"],["dc.identifier.isi","000374783300039"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/40723"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.publisher.place","Oxford"],["dc.relation.conference","25th Annual Meeting of the European-Chemoreception-Research-Organization (ECRO)"],["dc.relation.eventlocation","Bogazici Univ, Istanbul, TURKEY"],["dc.relation.issn","1464-3553"],["dc.relation.issn","0379-864X"],["dc.title","Optical dissection of pre- and postsynaptic plasticity in the olfactory system of Drosophila melanogaster."],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","952"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Cell Reports"],["dc.bibliographiccitation.lastpage","960"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Riemensperger, Thomas"],["dc.contributor.author","Issa, Abdul-Raouf"],["dc.contributor.author","Pech, Ulrike"],["dc.contributor.author","Coulom, Helene"],["dc.contributor.author","My-Van Nguyen, My-Van Nguyen"],["dc.contributor.author","Cassar, Marlene"],["dc.contributor.author","Jacquet, Melanie"],["dc.contributor.author","Fiala, Andre"],["dc.contributor.author","Birman, Serge"],["dc.date.accessioned","2018-11-07T09:17:49Z"],["dc.date.available","2018-11-07T09:17:49Z"],["dc.date.issued","2013"],["dc.description.abstract","Expression of the human Parkinson-disease-associated protein alpha-synuclein in all Drosophila neurons induces progressive locomotor deficits. Here, we identify a group of 15 dopaminergic neurons per hemisphere in the anterior medial region of the brain whose disruption correlates with climbing impairments in this model. These neurons selectively innervate the horizontal beta and beta' lobes of the mushroom bodies, and their connections to the Kenyon cells are markedly reduced when they express alpha-synuclein. Using selective mushroom body drivers, we show that blocking or overstimulating neuronal activity in the beta' lobe, but not the beta or gamma lobes, significantly inhibits negative geotaxis behavior. This suggests that modulation of the mushroom body beta' lobes by this dopaminergic pathway is specifically required for an efficient control of startle-induced locomotion in flies."],["dc.identifier.doi","10.1016/j.celrep.2013.10.032"],["dc.identifier.isi","000328266000011"],["dc.identifier.pmid","24239353"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10671"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28258"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.relation.issn","2211-1247"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","A Single Dopamine Pathway Underlies Progressive Locomotor Deficits in a Drosophila Model of Parkinson Disease"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Pooryasin, Atefeh"],["dc.contributor.author","Maglione, Marta"],["dc.contributor.author","Schubert, Marco"],["dc.contributor.author","Matkovic-Rachid, Tanja"],["dc.contributor.author","Hasheminasab, Sayed-mohammad"],["dc.contributor.author","Pech, Ulrike"],["dc.contributor.author","Fiala, André"],["dc.contributor.author","Mielke, Thorsten"],["dc.contributor.author","Sigrist, Stephan J."],["dc.date.accessioned","2021-06-01T09:41:40Z"],["dc.date.available","2021-06-01T09:41:40Z"],["dc.date.issued","2021"],["dc.description.abstract","Abstract The physical distance between presynaptic Ca 2+ channels and the Ca 2+ sensors triggering the release of neurotransmitter-containing vesicles regulates short-term plasticity (STP). While STP is highly diversified across synapse types, the computational and behavioral relevance of this diversity remains unclear. In the Drosophila brain, at nanoscale level, we can distinguish distinct coupling distances between Ca 2+ channels and the (m)unc13 family priming factors, Unc13A and Unc13B. Importantly, coupling distance defines release components with distinct STP characteristics. Here, we show that while Unc13A and Unc13B both contribute to synaptic signalling, they play distinct roles in neural decoding of olfactory information at excitatory projection neuron (ePN) output synapses. Unc13A clusters closer to Ca 2+ channels than Unc13B, specifically promoting fast phasic signal transfer. Reduction of Unc13A in ePNs attenuates responses to both aversive and appetitive stimuli, while reduction of Unc13B provokes a general shift towards appetitive values. Collectively, we provide direct genetic evidence that release components of distinct nanoscopic coupling distances differentially control STP to play distinct roles in neural decoding of sensory information."],["dc.identifier.doi","10.1038/s41467-021-22180-6"],["dc.identifier.pmid","33771998"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/84997"],["dc.identifier.url","https://for2705.de/literature/publications/38"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation","FOR 2705: Dissection of a Brain Circuit: Structure, Plasticity and Behavioral Function of the Drosophila Mushroom Body"],["dc.relation","FOR 2705 | TP 5: Postsynaptic receptor plasticity and transsynaptic communication in storage of memory components in the mushroom bodies"],["dc.relation.eissn","2041-1723"],["dc.relation.workinggroup","RG Fiala"],["dc.relation.workinggroup","RG Sigrist (Genetics)"],["dc.rights","CC BY 4.0"],["dc.title","Unc13A and Unc13B contribute to the decoding of distinct sensory information in Drosophila"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Journal of Neurogenetics"],["dc.bibliographiccitation.volume","26"],["dc.contributor.author","Pech, Ulrike"],["dc.contributor.author","Pooryasin, Atefeh"],["dc.contributor.author","Jauch, Mandy"],["dc.contributor.author","Fiala, Andre"],["dc.date.accessioned","2018-11-07T09:02:29Z"],["dc.date.available","2018-11-07T09:02:29Z"],["dc.date.issued","2012"],["dc.format.extent","63"],["dc.identifier.isi","000314975100159"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24691"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Informa Healthcare"],["dc.publisher.place","London"],["dc.relation.issn","0167-7063"],["dc.title","Analysis of camp signaling in olfactory transduction in Drosophila larvae"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2083"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Cell Reports"],["dc.bibliographiccitation.lastpage","2095"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Pech, Ulrike"],["dc.contributor.author","Revelo, Natalia H."],["dc.contributor.author","Seitz, Katharina J."],["dc.contributor.author","Rizzoli, S. O."],["dc.contributor.author","Fiala, Andre"],["dc.date.accessioned","2017-09-07T11:44:29Z"],["dc.date.available","2017-09-07T11:44:29Z"],["dc.date.issued","2015"],["dc.description.abstract","Drosophila represents a key model organism for dissecting neuronal circuits that underlie innate and adaptive behavior. However, this task is limited by a lack of tools to monitor physiological parameters of spatially distributed, central synapses in identified neurons. We generated transgenic fly strains that express functional fluorescent reporters targeted to either pre-or postsynaptic compartments. Presynaptic Ca2+ dynamics are monitored using synaptophysin-coupled GCaMP3, synaptic transmission is monitored using red fluorescent synaptophysinpHTomato, and postsynaptic Ca2+ dynamics are visualized usingGCaMP3fused with the postsynaptic matrix protein, dHomer. Using two-photon in vivo imaging of olfactory projection neurons, odor-evoked activity across populations of synapses is visualized in the antennal lobe and the mushroom body calyx. Prolonged odor exposure causes odor-specific and differential experience-dependent changes in preand postsynaptic activity at both levels of olfactory processing. The approach advances the physiological analysis of synaptic connections across defined groups of neurons in intact Drosophila."],["dc.identifier.doi","10.1016/j.celrep.2015.02.065"],["dc.identifier.gro","3141936"],["dc.identifier.isi","000352138400014"],["dc.identifier.pmid","25818295"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12108"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2724"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","2211-1247"],["dc.rights","CC BY-NC-ND 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-nd/3.0"],["dc.title","Optical Dissection of Experience-Dependent Pre- and Postsynaptic Plasticity in the Drosophila Brain"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]