Fiala, André

[["dc.bibliographiccitation.firstpage","ENEURO.0213-19.2019"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","eneuro"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Grabe, Veit"],["dc.contributor.author","Schubert, Marco"],["dc.contributor.author","Strube-Bloss, Martin"],["dc.contributor.author","Reinert, Anja"],["dc.contributor.author","Trautheim, Silke"],["dc.contributor.author","Lavista-Llanos, Sofia"],["dc.contributor.author","Fiala, André"],["dc.contributor.author","Hansson, Bill S."],["dc.contributor.author","Sachse, Silke"],["dc.date.accessioned","2021-04-14T08:27:23Z"],["dc.date.available","2021-04-14T08:27:23Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1523/ENEURO.0213-19.2019"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82277"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","2373-2822"],["dc.title","Odor-Induced Multi-Level Inhibitory Maps in Drosophila"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4511"],["dc.bibliographiccitation.issue","24"],["dc.bibliographiccitation.journal","The EMBO Journal"],["dc.bibliographiccitation.lastpage","4523"],["dc.bibliographiccitation.volume","31"],["dc.contributor.author","Kucherenko, Mariya M."],["dc.contributor.author","Barth, Jonas"],["dc.contributor.author","Fiala, Andre"],["dc.contributor.author","Shcherbata, Halyna R."],["dc.date.accessioned","2018-11-07T09:02:20Z"],["dc.date.available","2018-11-07T09:02:20Z"],["dc.date.issued","2012"],["dc.description.abstract","Mammalian neuronal stem cells produce multiple neuron types in the course of an individual's development. Similarly, neuronal progenitors in the Drosophila brain generate different types of closely related neurons that are born at specific time points during development. We found that in the post-embryonic Drosophila brain, steroid hormones act as temporal cues that specify the cell fate of mushroom body (MB) neuroblast progeny. Chronological regulation of neurogenesis is subsequently mediated by the microRNA (miRNA) let-7, absence of which causes learning impairment due to morphological MB defects. The miRNA let-7 is required to regulate the timing of alpha'/beta' to alpha/beta neuronal identity transition by targeting the transcription factor Abrupt. At a cellular level, the ecdysone-let-7-Ab signalling pathway controls the expression levels of the cell adhesion molecule Fasciclin II in developing neurons that ultimately influences their differentiation. Our data propose a novel role for miRNAs as transducers between chronologically regulated developmental signalling and physical cell adhesion. The EMBO Journal (2012) 31, 4511-4523. doi:10.1038/emboj.2012.298; Published online 16 November 2012"],["dc.description.sponsorship","Max Planck Society; German Research Council [SPP 1392 (FI 821/2-1)]"],["dc.identifier.doi","10.1038/emboj.2012.298"],["dc.identifier.isi","000312446700005"],["dc.identifier.pmid","23160410"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24658"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","0261-4189"],["dc.title","Steroid-induced microRNA let-7 acts as a spatio-temporal code for neuronal cell fate in the developing Drosophila brain"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1169"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Biochimica et Biophysica Acta (BBA) - General Subjects"],["dc.bibliographiccitation.lastpage","1178"],["dc.bibliographiccitation.volume","1820"],["dc.contributor.author","Riemensperger, Thomas"],["dc.contributor.author","Pech, Ulrike"],["dc.contributor.author","Dipt, Shubham"],["dc.contributor.author","Fiala, Andre"],["dc.date.accessioned","2018-11-07T09:08:06Z"],["dc.date.available","2018-11-07T09:08:06Z"],["dc.date.issued","2012"],["dc.description.abstract","Background: Drosophila melanogaster is one of the best-studied model organisms in biology, mainly because of the versatility of methods by which heredity and specific expression of genes can be traced and manipulated. Sophisticated genetic tools have been developed to express transgenes in selected cell types, and these techniques can be utilized to target DNA-encoded fluorescence probes to genetically defined subsets of neurons. Neuroscientists make use of this approach to monitor the activity of restricted types or subsets of neurons in the brain and the peripheral nervous system. Since membrane depolarization is typically accompanied by an increase in intracellular calcium ions, calcium-sensitive fluorescence proteins provide favorable tools to monitor the spatio-temporal activity across groups of neurons. Scope of review: Here we describe approaches to perform optical calcium imaging in Drosophila in consideration of various calcium sensors and expression systems. In addition, we outline by way of examples for which particular neuronal systems in Drosophila optical calcium imaging have been used. Finally, we exemplify briefly how optical calcium imaging in the brain of Drosophila can be carried out in practice. Major conclusions and general significance: Drosophila provides an excellent model organism to combine genetic expression systems with optical calcium imaging in order to investigate principles of sensory coding, neuronal plasticity, and processing of neuronal information underlying behavior. This article is part of a Special Issue entitled Biochemical, Biophysical and Genetic Approaches to Intracellular Calcium Signaling. (C) 2012 Elsevier B.V. All rights reserved."],["dc.identifier.doi","10.1016/j.bbagen.2012.02.013"],["dc.identifier.isi","000305595300003"],["dc.identifier.pmid","22402253"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/25948"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","0304-4165"],["dc.title","Optical calcium imaging in the nervous system of Drosophila melanogaster"],["dc.type","review"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3992"],["dc.bibliographiccitation.issue","17"],["dc.bibliographiccitation.journal","The Journal of Comparative Neurology"],["dc.bibliographiccitation.lastpage","4026"],["dc.bibliographiccitation.volume","521"],["dc.contributor.author","Pech, Ulrike"],["dc.contributor.author","Pooryasin, Atefeh"],["dc.contributor.author","Birman, Serge"],["dc.contributor.author","Fiala, Andre"],["dc.date.accessioned","2018-11-07T09:17:25Z"],["dc.date.available","2018-11-07T09:17:25Z"],["dc.date.issued","2013"],["dc.description.abstract","The mushroom body of the insect brain represents a neuronal circuit involved in the control of adaptive behavior, e.g., associative learning. Its function relies on the modulation of Kenyon cell activity or synaptic transmitter release by biogenic amines, e.g., octopamine, dopamine, or serotonin. Therefore, for a comprehensive understanding of the mushroom body, it is of interest not only to determine which modulatory neurons interact with Kenyon cells but also to pinpoint where exactly in the mushroom body they do so. To accomplish the latter, we made use of the GRASP technique and created transgenic Drosophila melanogaster that carry one part of a membrane-bound splitGFP in Kenyon cells, along with a cytosolic red fluorescent marker. The second part of the splitGFP is expressed in distinct neuronal populations using cell-specific Gal4 drivers. GFP is reconstituted only if these neurons interact with Kenyon cells in close proximity, which, in combination with two-photon microscopy, provides a very high spatial resolution. We characterize spatially and microstructurally distinct contact regions between Kenyon cells and dopaminergic, serotonergic, and octopaminergic/tyraminergic neurons in all subdivisions of the mushroom body. Subpopulations of dopaminergic neurons contact complementary lobe regions densely. Octopaminergic/tyraminergic neurons contact Kenyon cells sparsely and are restricted mainly to the calyx, the -lobes, and the -lobes. Contacts of Kenyon cells with serotonergic neurons are heterogeneously distributed over the entire mushroom body. In summary, the technique enables us to localize precisely a segmentation of the mushroom body by differential contacts with aminergic neurons. J. Comp. Neurol. 521:3992-4026, 2013. (c) 2013 Wiley Periodicals, Inc."],["dc.identifier.doi","10.1002/cne.23388"],["dc.identifier.isi","000325461300008"],["dc.identifier.pmid","23784863"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28161"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","0021-9967"],["dc.title","Localization of the Contacts Between Kenyon Cells and Aminergic Neurons in the Drosophila melanogaster Brain Using SplitGFP Reconstitution"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","220096"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Open Biology"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Poppinga, Haiko"],["dc.contributor.author","Çoban, Büşra"],["dc.contributor.author","Meltzer, Hagar"],["dc.contributor.author","Mayseless, Oded"],["dc.contributor.author","Widmann, Annekathrin"],["dc.contributor.author","Schuldiner, Oren"],["dc.contributor.author","Fiala, André"],["dc.date.accessioned","2022-10-04T10:22:15Z"],["dc.date.available","2022-10-04T10:22:15Z"],["dc.date.issued","2022"],["dc.description.abstract","The principles of how brain circuits establish themselves during development are largely conserved across animal species. Connections made during embryonic development that are appropriate for an early life stage are frequently remodelled later in ontogeny via pruning and subsequent regrowth to generate adult-specific connectivity. The mushroom body of the fruit fly\n Drosophila melanogaster\n is a well-established model circuit for examining the cellular mechanisms underlying neurite remodelling. This central brain circuit integrates sensory information with learned and innate valences to adaptively instruct behavioural decisions. Thereby, the mushroom body organizes adaptive behaviour, such as associative learning. However, little is known about the specific aspects of behaviour that require mushroom body remodelling. Here, we used genetic interventions to prevent the intrinsic neurons of the larval mushroom body (γ-type Kenyon cells) from remodelling. We asked to what degree remodelling deficits resulted in impaired behaviour. We found that deficits caused hyperactivity and mild impairment in differential aversive olfactory learning, but not appetitive learning. Maintenance of circadian rhythm and sleep were not affected. We conclude that neurite pruning and regrowth of γ-type Kenyon cells is not required for the establishment of circuits that mediate associative odour learning\n per se\n , but it does improve distinct learning tasks."],["dc.description.sponsorship","European Research Council"],["dc.description.sponsorship"," German Research Foundation 501100001659"],["dc.description.sponsorship","Ministry of Science and Culture of Lower Saxony and Volkswagen Foundation"],["dc.identifier.doi","10.1098/rsob.220096"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/114622"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-600"],["dc.relation.eissn","2046-2441"],["dc.rights.uri","https://royalsociety.org/journals/ethics-policies/data-sharing-mining/"],["dc.title","Pruning deficits of the developing\n Drosophila\n mushroom body result in mild impairment in associative odour learning and cause hyperactivity"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","298"],["dc.bibliographiccitation.issue","3-4"],["dc.bibliographiccitation.journal","Journal of Neurogenetics"],["dc.bibliographiccitation.lastpage","305"],["dc.bibliographiccitation.volume","26"],["dc.contributor.author","Nuwal, Nidhi"],["dc.contributor.author","Stock, Patrick"],["dc.contributor.author","Hiemeyer, Jochen"],["dc.contributor.author","Schmid, Benjamin"],["dc.contributor.author","Fiala, Andre"],["dc.contributor.author","Buchner, Erich"],["dc.date.accessioned","2018-11-07T09:06:05Z"],["dc.date.available","2018-11-07T09:06:05Z"],["dc.date.issued","2012"],["dc.description.abstract","Animals have to perform adequate behavioral actions dependent on internal states and environmental situations, and adjust their behavior according to positive or negative consequences. The fruit fly Drosophila melanogaster represents a key model organism for the investigation of neuronal mechanisms underlying adaptive behavior. The authors are using a behavioral paradigm in which fruit flies attached to a manipulator can walk on a Styrofoam ball whose movements are recorded such that intended left or right turns of the flies can be registered and used to operantly control heat stimuli or optogenetic activation of distinct subsets of neurons. As proof of principle, the authors find that flies in this situation avoid heat stimuli but prefer optogenetic self-stimulation of sugar receptors. Using this setup it now should be possible to study the neuronal network underlying positive and negative value assessment of adult Drosophila in an operant setting."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft [SFB 554, FI 821/3-1]"],["dc.identifier.doi","10.3109/01677063.2012.700266"],["dc.identifier.isi","000311679300010"],["dc.identifier.pmid","22834571"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/25476"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Informa Healthcare"],["dc.relation.issn","0167-7063"],["dc.title","Avoidance of Heat and Attraction to Optogenetically Induced Sugar Sensation as Operant Behavior in Adult Drosophila"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","12792"],["dc.bibliographiccitation.issue","37"],["dc.bibliographiccitation.journal","Journal of Neuroscience"],["dc.bibliographiccitation.lastpage","12812"],["dc.bibliographiccitation.volume","35"],["dc.contributor.author","Pooryasin, Atefeh"],["dc.contributor.author","Fiala, Andre"],["dc.date.accessioned","2018-11-07T09:51:36Z"],["dc.date.available","2018-11-07T09:51:36Z"],["dc.date.issued","2015"],["dc.description.abstract","Animals show different levels of activity that are reflected in sensory responsiveness and endogenously generated behaviors. Biogenic amines have been determined to be causal factors for these states of arousal. It is well established that, in Drosophila, dopamine and octopamine promote increased arousal. However, little is known about factors that regulate arousal negatively and induce states of quiescence. Moreover, it remains unclear whether global, diffuse modulatory systems comprehensively affecting brain activity determine general states of arousal. Alternatively, individual aminergic neurons might selectively modulate the animals' activity in a distinct behavioral context. Here, we show that artificially activating large populations of serotonin-releasing neurons induces behavioral quiescence and inhibits feeding and mating. We systematically narrowed down a role of serotonin in inhibiting endogenously generated locomotor activity to neurons located in the posterior medial protocerebrum. We identified neurons of this cell cluster that suppress mating, but not feeding behavior. These results suggest that serotonin does not uniformly act as global, negative modulator of general arousal. Rather, distinct serotoninergic neurons can act as inhibitory modulators of specific behaviors."],["dc.description.sponsorship","German Research Foundation [FI 821/3-1]"],["dc.identifier.doi","10.1523/JNEUROSCI.1638-15.2015"],["dc.identifier.isi","000363659500017"],["dc.identifier.pmid","26377467"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/35947"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Soc Neuroscience"],["dc.relation.issn","0270-6474"],["dc.title","Identified Serotonin-Releasing Neurons Induce Behavioral Quiescence and Suppress Mating in Drosophila"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1819"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Journal of Neuroscience"],["dc.bibliographiccitation.lastpage","1837"],["dc.bibliographiccitation.volume","34"],["dc.contributor.author","Barth, Jonas"],["dc.contributor.author","Dipt, Shubham"],["dc.contributor.author","Pech, Ulrike"],["dc.contributor.author","Hermann, Moritz"],["dc.contributor.author","Riemensperger, Thomas"],["dc.contributor.author","Fiala, Andre"],["dc.date.accessioned","2018-11-07T09:44:54Z"],["dc.date.available","2018-11-07T09:44:54Z"],["dc.date.issued","2014"],["dc.description.abstract","Training can improve the ability to discriminate between similar, confusable stimuli, including odors. One possibility of enhancing behaviorally expressed discrimination (i.e., sensory acuity) relies on differential associative learning, during which animals are forced to detect the differences between similar stimuli. Drosophila represents a key model organism for analyzing neuronal mechanisms underlying both odor processing and olfactory learning. However, the ability of flies to enhance fine discrimination between similar odors through differential associative learning has not been analyzed in detail. We performed associative conditioning experiments using chemically similar odorants that we show to evoke overlapping neuronal activity in the fly's antennal lobes and highly correlated activity in mushroom body lobes. We compared the animals' performance in discriminating between these odors after subjecting them to one of two types of training: either absolute conditioning, in which only one odor is reinforced, or differential conditioning, in which one odor is reinforced and a second odor is explicitly not reinforced. First, we show that differential conditioning decreases behavioral generalization of similar odorants in a choice situation. Second, we demonstrate that this learned enhancement in olfactory acuity relies on both conditioned excitation and conditioned inhibition. Third, inhibitory local interneurons in the antennal lobes are shown to be required for behavioral fine discrimination between the two similar odors. Fourth, differential, but not absolute, training causes decorrelation of odor representations in the mushroom body. In conclusion, differential training with similar odors ultimately induces a behaviorally expressed contrast enhancement between the two similar stimuli that facilitates fine discrimination."],["dc.identifier.doi","10.1523/JNEUROSCI.2598-13.2014"],["dc.identifier.isi","000331455000024"],["dc.identifier.pmid","24478363"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/34499"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Soc Neuroscience"],["dc.relation.issn","0270-6474"],["dc.title","Differential Associative Training Enhances Olfactory Acuity in Drosophila melanogaster"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","52"],["dc.bibliographiccitation.journal","Journal of Neurogenetics"],["dc.bibliographiccitation.lastpage","53"],["dc.bibliographiccitation.volume","26"],["dc.contributor.author","Pooryasin, Atefeh"],["dc.contributor.author","Fiala, Andre"],["dc.date.accessioned","2018-11-07T09:02:27Z"],["dc.date.available","2018-11-07T09:02:27Z"],["dc.date.issued","2012"],["dc.identifier.isi","000314975100132"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24686"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Informa Healthcare"],["dc.publisher.place","London"],["dc.relation.issn","0167-7063"],["dc.title","A thermogenetic approach to analyze neuronal functions in Drosophila"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Chemical Senses"],["dc.bibliographiccitation.volume","41"],["dc.contributor.author","Fiala, Andre"],["dc.contributor.author","Pech, Ulrike"],["dc.date.accessioned","2018-11-07T10:14:57Z"],["dc.date.available","2018-11-07T10:14:57Z"],["dc.date.issued","2016"],["dc.format.extent","388"],["dc.identifier.isi","000374783300039"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/40723"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.publisher.place","Oxford"],["dc.relation.conference","25th Annual Meeting of the European-Chemoreception-Research-Organization (ECRO)"],["dc.relation.eventlocation","Bogazici Univ, Istanbul, TURKEY"],["dc.relation.issn","1464-3553"],["dc.relation.issn","0379-864X"],["dc.title","Optical dissection of pre- and postsynaptic plasticity in the olfactory system of Drosophila melanogaster."],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]