Rehfeldt, Florian

[["dc.bibliographiccitation.journal","eLife"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Narayanan, Pratibha"],["dc.contributor.author","Hütte, Meike"],["dc.contributor.author","Kudryasheva, Galina"],["dc.contributor.author","Taberner, Francisco J"],["dc.contributor.author","Lechner, Stefan G"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Gomez-Varela, David"],["dc.contributor.author","Schmidt, Manuela"],["dc.date.accessioned","2020-12-10T18:48:06Z"],["dc.date.available","2020-12-10T18:48:06Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.7554/eLife.32346"],["dc.identifier.eissn","2050-084X"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/79015"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Myotubularin related protein-2 and its phospholipid substrate PIP2 control Piezo2-mediated mechanotransduction in peripheral sensory neurons"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4294"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","ACS Sustainable Chemistry & Engineering"],["dc.bibliographiccitation.lastpage","4301"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Huang, Heqin"],["dc.contributor.author","Wang, Xiaojie"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Zhang, Kai"],["dc.date.accessioned","2020-05-18T10:10:36Z"],["dc.date.available","2020-05-18T10:10:36Z"],["dc.date.issued","2018"],["dc.description.abstract","Biological tissues are often highly and multiply heterogeneous in both structure and composition, but the integrity of multiheterogeneity in artificial materials is still a big challenge. Herein, dually heterogeneous hydrogels were constructed with two distinct strategies via dynamic bonds and supramolecular cross-links. The hydrogels showed discontinuous spatial ruptures, and the mechanical behaviors of hydrogels could be tuned. The primary heterogeneity resulted from a nonuniform distribution of dynamic and/or static cross-links. The presence of only primary heterogeneity within hydrogels led to uneven mechanical properties that were represented by discontinuous spatial ruptures during the stretching the hydrogel and therefore caused the necking deformation. Further introduction of the secondary heterogeneity by incorporating anisotropic cellulose nanocrystals (CNC) into the hydrogels allowed the adjustment of the necking phenomenon. Moreover, distinct CNC with diverse surface functionalities exhibited different effects: the “active” CNC with surface-attached dynamic bonds retarded the necking propagation, while the “neutral” CNC without further surface modification promoted the extension of necking points. Thus, the regulation of deformation and fracture mode of hydrogels was achieved by the synergy of dually heterogeneous structure."],["dc.identifier.doi","10.1021/acssuschemeng.7b04738"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/65514"],["dc.language.iso","en"],["dc.title","Dually Heterogeneous Hydrogels via Dynamic and Supramolecular Cross-Links Tuning Discontinuous Spatial Ruptures"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0161623"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Prabhune, Meenakshi"],["dc.contributor.author","von Roden, Kerstin"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Schmidt, Christoph"],["dc.date.accessioned","2017-09-07T11:44:43Z"],["dc.date.available","2017-09-07T11:44:43Z"],["dc.date.issued","2016"],["dc.description.abstract","Microtubule structure and functions have been widely studied in vitro and in cells. Research has shown that cysteines on tubulin play a crucial role in the polymerization of microtubules. Here, we show that blocking sulfhydryl groups of cysteines in taxol-stabilized polymerized microtubules with a commonly used chemical crosslinker prevents temporal end-to-end annealing of microtubules in vitro. This can dramatically affect the length distribution of the microtubules. The crosslinker sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate, sulfo-SMCC, consists of a maleimide and an N-hydroxysuccinimide ester group to bind to sulfhydryl groups and primary amines, respectively. Interestingly, addition of a maleimide dye alone does not show the same interference with annealing in stabilized microtubules. This study shows that the sulfhydryl groups of cysteines of tubulin that are vital for the polymerization are also important for the subsequent annealing of microtubules."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2016"],["dc.identifier.doi","10.1371/journal.pone.0161623"],["dc.identifier.gro","3141632"],["dc.identifier.isi","000382258600066"],["dc.identifier.pmid","27561096"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13701"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/3345"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.relation.orgunit","Fakultät für Physik"],["dc.rights","CC BY 4.0"],["dc.title","Sulfo-SMCC Prevents Annealing of Taxol-Stabilized Microtubules In Vitro"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","51"],["dc.bibliographiccitation.journal","Progress in Biophysics and Molecular Biology"],["dc.bibliographiccitation.lastpage","60"],["dc.bibliographiccitation.volume","144"],["dc.contributor.author","Schlick, Susanne F."],["dc.contributor.author","Spreckelsen, Florian"],["dc.contributor.author","Tiburcy, Malte"],["dc.contributor.author","Iyer, Lavanya M."],["dc.contributor.author","Meyer, Tim"],["dc.contributor.author","Zelarayan, Laura C."],["dc.contributor.author","Luther, Stefan"],["dc.contributor.author","Parlitz, Ulrich"],["dc.contributor.author","Zimmermann, Wolfram-Hubertus"],["dc.contributor.author","Rehfeldt, Florian"],["dc.date.accessioned","2020-12-10T15:20:42Z"],["dc.date.available","2020-12-10T15:20:42Z"],["dc.date.issued","2019"],["dc.description.abstract","Cardiomyocyte and stroma cell cross-talk is essential for the formation of collagen-based engineered heart muscle, including engineered human myocardium (EHM). Fibroblasts are a main component of the myocardial stroma. We hypothesize that fibroblasts, by compacting the surrounding collagen network, support the self-organization of cardiomyocytes into a functional syncytium. With a focus on early self-organization processes in EHM, we studied the molecular and biophysical adaptations mediated by defined populations of fibroblasts and embryonic stem cell-derived cardiomyocytes in a collagen type I hydrogel. After a short phase of cell-independent collagen gelation (30 min), tissue compaction was progressively mediated by fibroblasts. Fibroblast-mediated tissue stiffening was attenuated in the presence of cardiomyocytes allowing for the assembly of stably contracting, force-generating EHM within 4 weeks. Comparative RNA-sequencing data corroborated that fibroblasts are particularly sensitive to the tissue compaction process, resulting in the fast activation of transcription profiles, supporting heart muscle development and extracellular matrix synthesis. Large amplitude oscillatory shear (LAOS) measurements revealed nonlinear strain stiffening at physiological strain amplitudes (>2%), which was reduced in the presence of cells. The nonlinear stress-strain response could be characterized by a mathematical model. Collectively, our study defines the interplay between fibroblasts and cardiomyocytes during human heart muscle self-organization in vitro and underscores the relevance of fibroblasts in the biological engineering of a cardiomyogenesis-supporting viscoelastic stroma. We anticipate that the established mathematical model will facilitate future attempts to optimize EHM for in vitro (disease modelling) and in vivo applications (heart repair)."],["dc.identifier.doi","10.1016/j.pbiomolbio.2018.11.011"],["dc.identifier.pmid","30553553"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/72769"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/248"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | C04: Fibroblasten-Kardiomyozyten Interaktion im gesunden und erkrankten Herzen: Mechanismen und therapeutische Interventionen bei Kardiofibroblastopathien"],["dc.relation","SFB 1002 | S01: In vivo und in vitro Krankheitsmodelle"],["dc.relation.workinggroup","RG Luther (Biomedical Physics)"],["dc.relation.workinggroup","RG Tiburcy (Stem Cell Disease Modeling)"],["dc.relation.workinggroup","RG Zelarayán-Behrend (Developmental Pharmacology)"],["dc.relation.workinggroup","RG Zimmermann (Engineered Human Myocardium)"],["dc.rights","CC BY 4.0"],["dc.title","Agonistic and antagonistic roles of fibroblasts and cardiomyocytes on viscoelastic stiffening of engineered human myocardium"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","463001"],["dc.bibliographiccitation.issue","46"],["dc.bibliographiccitation.journal","Journal of Physics D: Applied Physics"],["dc.bibliographiccitation.volume","50"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Schmidt, Christoph F."],["dc.date.accessioned","2019-07-09T11:44:30Z"],["dc.date.available","2019-07-09T11:44:30Z"],["dc.date.issued","2017"],["dc.description.abstract","In the last two decades, it has become evident that the mechanical properties of the microenvironment of biological cells are as important as traditional biochemical cues for the control of cellular behavior and fate. The field of cell and matrix mechanics is quickly growing and so is the development of the experimental approaches used to study active and passive mechanical properties of cells and their surroundings. Within this topical review we will provide a brief overview, on the one hand, over how cellular mechanics can be probed physically, how different geometries allow access to different cellular properties, and, on the other hand, how forces are generated in cells and transmitted to the extracellular environment. We will describe the following experimental techniques: atomic force microscopy, traction force microscopy, magnetic tweezers, optical stretcher and optical tweezers pointing out both their advantages and limitations. Finally, we give an outlook on the future of the physical probing of cells."],["dc.identifier.doi","10.1088/1361-6463/aa8aa6"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14805"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59027"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation","info:eu-repo/grantAgreement/EC/FP7/340528/EU//CELLMECHANOCONTROL"],["dc.relation.issn","1361-6463"],["dc.relation.orgunit","Fakultät für Physik"],["dc.rights","CC BY 4.0"],["dc.subject.ddc","530"],["dc.title","Physical probing of cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","263"],["dc.bibliographiccitation.lastpage","282"],["dc.bibliographiccitation.seriesnr","134"],["dc.contributor.author","Eltzner, Benjamin"],["dc.contributor.author","Hauke, Lara"],["dc.contributor.author","Huckemann, Stephan"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Wollnik, Carina"],["dc.contributor.editor","Salditt, Tim"],["dc.contributor.editor","Egner, Alexander"],["dc.contributor.editor","Luke, D. Russell"],["dc.date.accessioned","2021-04-21T11:15:39Z"],["dc.date.available","2021-04-21T11:15:39Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1007/978-3-030-34413-9_10"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/84280"],["dc.relation","SFB 755: Nanoscale Photonic Imaging"],["dc.relation.crisseries","Topics in Applied Physics"],["dc.relation.doi","10.1007/978-3-030-34413-9"],["dc.relation.eisbn","978-3-030-34413-9"],["dc.relation.isbn","978-3-030-34412-2"],["dc.relation.ispartof","Nanoscale Photonic Imaging"],["dc.relation.ispartofseries","Topics in Applied Physics; 134"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.subject.gro","SFB 755"],["dc.title","A Statistical and Biophysical Toolbox to Elucidate Structure and Formation of Stress Fibers"],["dc.type","book_chapter"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Molecular Biology of the Cell"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Harada, T."],["dc.contributor.author","Brown, A. E. X."],["dc.contributor.author","Zajac, Allison L."],["dc.contributor.author","Cai, Shenshen"],["dc.contributor.author","Zemel, Assaf"],["dc.contributor.author","Discher, Dennis E."],["dc.date.accessioned","2018-11-07T09:00:13Z"],["dc.date.available","2018-11-07T09:00:13Z"],["dc.date.issued","2011"],["dc.identifier.isi","000305505504181"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24099"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Cell Biology"],["dc.publisher.place","Bethesda"],["dc.relation.conference","Annual Meeting of the American-Society-for-Cell-Biology (ASCB)"],["dc.relation.eventlocation","Denver, CO"],["dc.relation.issn","1059-1524"],["dc.title","Matrix Stiffness Dictates Cytoskeletal Organization and Nuclear Morphology in 2D and 3D."],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Molecular Biology of the Cell"],["dc.bibliographiccitation.volume","24"],["dc.contributor.author","Wollnik, Carina"],["dc.contributor.author","Kudryasheva, Galina"],["dc.contributor.author","Radwitz, J."],["dc.contributor.author","Rehfeldt, Florian"],["dc.date.accessioned","2018-11-07T09:31:01Z"],["dc.date.available","2018-11-07T09:31:01Z"],["dc.date.issued","2013"],["dc.identifier.isi","000209348700271"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31445"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Cell Biology"],["dc.publisher.place","Bethesda"],["dc.relation.issn","1939-4586"],["dc.relation.issn","1059-1524"],["dc.title","Cytoskeletal Structures in Stem Cells as Mechano-Sensors"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","397"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Investigative Ophthalmology & Visual Science"],["dc.bibliographiccitation.lastpage","406"],["dc.bibliographiccitation.volume","60"],["dc.contributor.author","Gottschalk, Hanna M."],["dc.contributor.author","Wecker, Thomas"],["dc.contributor.author","Khattab, Mohammed H."],["dc.contributor.author","Fischer, Charlotte V."],["dc.contributor.author","Callizo, Josep"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Lubjuhn, Roswitha"],["dc.contributor.author","Russmann, Christoph"],["dc.contributor.author","Hoerauf, Hans"],["dc.contributor.author","van Oterendorp, Christian"],["dc.date.accessioned","2019-07-09T11:50:10Z"],["dc.date.available","2019-07-09T11:50:10Z"],["dc.date.issued","2019"],["dc.description.abstract","Purpose: Contrast agents applicable for optical coherence tomography (OCT) imaging are rare. The intrascleral aqueous drainage system would be a potential application for a contrast agent, because the aqueous veins are of small diameter and located deep inside the highly scattering sclera. We tested lipid emulsions (LEs) as candidate OCT contrast agents in vitro and ex vivo, including milk and the anesthetic substance Propofol. Methods: Commercial OCT and OCT angiography (OCTA) devices were used. Maximum reflectivity and signal transmission of LE were determined in tube phantoms. Absorption spectra and light scattering was analyzed. The anterior chamber of enucleated porcine eyes was perfused with LEs, and OCTA imaging of the LEs drained via the aqueous outflow tract was performed. Results: All LEs showed a significantly higher reflectivity than water (P < 0.001). Higher milk lipid content was positively correlated with maximum reflectivity and negatively with signal transmission. Propofol exhibited the best overall performance. Due to a high degree of signal fluctuation, OCTA could be applied for detection of LE. Compared with blood, the OCTA signal of Propofol was significantly stronger (P = 0.001). As a proof of concept, time-resolved aqueous angiography of porcine eyes was performed. The three-dimensional (3D) structure and dynamics of the aqueous outflow were significantly different from humans. Conclusions: LEs induced a strong signal in OCT and OCTA. LE-based OCTA allowed the ability to obtain time-resolved 3D datasets of aqueous outflow. Possible interactions of LE with inner eye's structures need to be further investigated before in vivo application."],["dc.identifier.doi","10.1167/iovs.18-25223"],["dc.identifier.pmid","30682210"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15874"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59715"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1552-5783"],["dc.rights","CC BY-NC-ND 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-nd/4.0"],["dc.subject.ddc","610"],["dc.title","Lipid Emulsion-Based OCT Angiography for Ex Vivo Imaging of the Aqueous Outflow Tract."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","551"],["dc.bibliographiccitation.journal","Frontiers in Physiology"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Kaliman, Sara"],["dc.contributor.author","Jayachandran, Christina"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Smith, Ana-Sunčana"],["dc.date.accessioned","2019-07-09T11:42:54Z"],["dc.date.available","2019-07-09T11:42:54Z"],["dc.date.issued","2016"],["dc.description.abstract","It is well accepted that cells in the tissue can be regarded as tiles tessellating space. A number of approaches were developed to find an appropriate mathematical description of such cell tiling. A particularly useful approach is the so called Voronoi tessellation, built from centers of mass of the cell nuclei (CMVT), which is commonly used for estimating the morphology of cells in epithelial tissues. However, a study providing a statistically sound analysis of this method's accuracy is not available in the literature. We addressed this issue here by comparing a number of morphological measures of the cells, including area, perimeter, and elongation obtained from such a tessellation with identical measures extracted from direct imaging acquired by staining the cell membranes. After analyzing the shapes of 15,000 MDCK II epithelial cells under several conditions, we find that CMVT reasonably well reproduces many of the morphological properties of the tissue with an error that is between 10 and 15%. Moreover, cross-correlations between different morphological measures are reproduced qualitatively correctly by this method. However, all of the properties including the cell perimeters, number of neighbors, and anisotropy measures often suffer from systematic or size dependent errors. These discrepancies originate from the polygonal nature of the tessellation which sets the limits of the applicability of CMVT."],["dc.identifier.doi","10.3389/fphys.2016.00551"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13989"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58782"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","Frontiers Media S.A."],["dc.relation","info:eu-repo/grantAgreement/EC/FP7/337283/EU/Biological Membranes in Action: A Unified Approach to Complexation, Scaffolding and Active Transport/MEMBRANESACT"],["dc.relation.eissn","1664-042X"],["dc.relation.issn","1664-042X"],["dc.relation.orgunit","Fakultät für Physik"],["dc.rights","CC BY 4.0"],["dc.subject.ddc","530"],["dc.title","Limits of Applicability of the Voronoi Tessellation Determined by Centers of Cell Nuclei to Epithelium Morphology"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]