Urban, Nicolai T.

[["dc.bibliographiccitation.artnumber","7368"],["dc.bibliographiccitation.firstpage","204"],["dc.bibliographiccitation.journal","Nature"],["dc.bibliographiccitation.lastpage","208"],["dc.bibliographiccitation.volume","478"],["dc.contributor.author","Grotjohann, Tim"],["dc.contributor.author","Testa, Ilaria"],["dc.contributor.author","Leutenegger, Marcel"],["dc.contributor.author","Bock, Hannes"],["dc.contributor.author","Urban, Nicolai T."],["dc.contributor.author","Lavoie-Cardinal, Flavie"],["dc.contributor.author","Willig, Katrin I."],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2017-09-07T11:43:21Z"],["dc.date.available","2017-09-07T11:43:21Z"],["dc.date.issued","2011"],["dc.description.abstract","Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for decades, but the recent breaking of the diffraction resolution barrier by sequentially switching the fluorescence capability of adjacent features on and off is making nanoscale imaging routine. Reported fluorescence nanoscopy variants switch these features either with intense beams at defined positions or randomly, molecule by molecule. Here we demonstrate an optical nanoscopy that records raw data images from living cells and tissues with low levels of light. This advance has been facilitated by the generation of reversibly switchable enhanced green fluorescent protein (rsEGFP), a fluorescent protein that can be reversibly photoswitched more than a thousand times. Distributions of functional rsEGFP-fusion proteins in living bacteria and mammalian cells are imaged at <40-nanometre resolution. Dendritic spines in living brain slices are super-resolved with about a million times lower light intensities than before. The reversible switching also enables all-optical writing of features with subdiffraction size and spacings, which can be used for data storage."],["dc.identifier.doi","10.1038/nature10497"],["dc.identifier.gro","3142644"],["dc.identifier.isi","000295782800041"],["dc.identifier.pmid","21909116"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0028-0836"],["dc.title","Diffraction-unlimited all-optical imaging and writing with a photochromic GFP"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","290"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Neef, Jakob"],["dc.contributor.author","Urban, Nicolai T."],["dc.contributor.author","Ohn, Tzu-Lun"],["dc.contributor.author","Frank, Thomas"],["dc.contributor.author","Jean, Philippe"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Willig, Katrin I."],["dc.contributor.author","Moser, Tobias"],["dc.date.accessioned","2018-04-23T11:48:23Z"],["dc.date.available","2018-04-23T11:48:23Z"],["dc.date.issued","2018"],["dc.description.abstract","Ca2+ influx triggers the release of synaptic vesicles at the presynaptic active zone (AZ). A quantitative characterization of presynaptic Ca2+ signaling is critical for understanding synaptic transmission. However, this has remained challenging to establish at the required resolution. Here, we employ confocal and stimulated emission depletion (STED) microscopy to quantify the number (20–330) and arrangement (mostly linear 70 nm × 100–600 nm clusters) of Ca2+ channels at AZs of mouse cochlear inner hair cells (IHCs). Establishing STED Ca2+ imaging, we analyze presynaptic Ca2+ signals at the nanometer scale and find confined elongated Ca2+ domains at normal IHC AZs, whereas Ca2+ domains are spatially spread out at the AZs of bassoon-deficient IHCs. Performing 2D-STED fluorescence lifetime analysis, we arrive at estimates of the Ca2+ concentrations at stimulated IHC AZs of on average 25 µM. We propose that IHCs form bassoon-dependent presynaptic Ca2+-channel clusters of similar density but scalable length, thereby varying the number of Ca2+ channels amongst individual AZs."],["dc.identifier.doi","10.1038/s41467-017-02612-y"],["dc.identifier.gro","3142361"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15588"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13498"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation.issn","2041-1723"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Quantitative optical nanophysiology of Ca2+ signaling at inner hair cell active zones"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","992"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Neuron"],["dc.bibliographiccitation.lastpage","1000"],["dc.bibliographiccitation.volume","75"],["dc.contributor.author","Testa, Ilaria"],["dc.contributor.author","Urban, Nicolai T."],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Willig, Katrin I."],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:48:25Z"],["dc.date.available","2017-09-07T11:48:25Z"],["dc.date.issued","2012"],["dc.description.abstract","Lens-based fluorescence microscopy, which has long been limited in resolution to about 200 nanometers by diffraction, is rapidly evolving into a nanoscale imaging technique. Here, we show that the superresolution fluorescence microscopy called RESOLFT enables comparatively fast and continuous imaging of sensitive, nanosized features in living brain tissue. Using low-intensity illumination to switch photochromic fluorescent proteins reversibly between a fluorescent and a nonfluorescent state, we increased the resolution more than 3-fold over that of confocal microscopy in all dimensions. Dendritic spines located 10-50 mu m deep inside living organotypic hippocampal brain slices were recorded for hours without signs of degradation. Using a fast-switching protein increased the imaging speed 50-fold over reported RESOLFT schemes, which in turn enabled the recording of spontaneous and stimulated changes of dendritic actin filaments and spine morphology occurring on time scales from seconds to hours."],["dc.identifier.doi","10.1016/j.neuron.2012.07.028"],["dc.identifier.gro","3142464"],["dc.identifier.isi","000309198900011"],["dc.identifier.pmid","22998868"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/8574"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Cell Press"],["dc.relation.issn","0896-6273"],["dc.title","Nanoscopy of Living Brain Slices with Low Light Levels"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","E8047"],["dc.bibliographiccitation.issue","34"],["dc.bibliographiccitation.journal","Proceedings of the National Academy of Sciences of the United States of America"],["dc.bibliographiccitation.lastpage","E8056"],["dc.bibliographiccitation.volume","115"],["dc.contributor.author","Masch, Jennifer-Magdalena"],["dc.contributor.author","Steffens, Heinz"],["dc.contributor.author","Fischer, Joachim"],["dc.contributor.author","Engelhardt, Johann"],["dc.contributor.author","Hubrich, Jasmine"],["dc.contributor.author","Keller-Findeisen, Jan"],["dc.contributor.author","D'Este, Elisa"],["dc.contributor.author","Urban, Nicolai T."],["dc.contributor.author","Grant, Seth G. N."],["dc.contributor.author","Sahl, Steffen J."],["dc.contributor.author","Kamin, Dirk"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2018-11-16T10:48:20Z"],["dc.date.accessioned","2021-10-27T13:21:10Z"],["dc.date.available","2018-11-16T10:48:20Z"],["dc.date.available","2021-10-27T13:21:10Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1073/pnas.1807104115"],["dc.identifier.pmid","30082388"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15631"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/91999"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/37"],["dc.language.iso","en"],["dc.notes.intern","Migrated from goescholar"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation","SFB 1286 | A07: Der Aufbau des synaptischen Cytoskeletts"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.relation.workinggroup","RG D’Este"],["dc.relation.workinggroup","RG Hell"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Robust nanoscopy of a synaptic protein in living mice by organic-fluorophore labeling"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","534a"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","104"],["dc.contributor.author","Testa, Ilaria"],["dc.contributor.author","Urban, Nicolai"],["dc.contributor.author","Willig, Katrin"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2022-03-01T11:44:56Z"],["dc.date.available","2022-03-01T11:44:56Z"],["dc.date.issued","2013"],["dc.identifier.doi","10.1016/j.bpj.2012.11.2958"],["dc.identifier.pii","S000634951204204X"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103168"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.issn","0006-3495"],["dc.title","Resolft Nanoscopy in Life Sciences: Unraveling Fine Details with Low Light Levels"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","122"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Nature Photonics"],["dc.bibliographiccitation.lastpage","128"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Danzl, Johann G."],["dc.contributor.author","Sidenstein, Sven C."],["dc.contributor.author","Gregor, Carola"],["dc.contributor.author","Urban, Nicolai T."],["dc.contributor.author","Ilgen, Peter"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2017-09-07T11:54:41Z"],["dc.date.available","2017-09-07T11:54:41Z"],["dc.date.issued","2016"],["dc.description.abstract","Far-field super-resolution fluorescence microscopy discerns fluorophores residing closer than the diffraction barrier by briefly transferring them in different (typically ON and OFF) states before detection. In coordinate-targeted super-resolution variants, such as stimulated emission depletion (STED) microscopy, this state difference is created by the intensity minima and maxima of an optical pattern, causing all fluorophores to assume the off state, for instance, except at the minima. Although strong spatial confinement of the on state enables high resolution, it also subjects the fluorophores to excess intensities and state cycles at the maxima. Here, we address these issues by driving the fluorophores into a second off state that is inert to the excess light. By using reversibly switchable fluorescent proteins as labels, our approach reduces bleaching and enhances resolution and contrast in live-cell STED microscopy. Using two or more transitions to off states is a useful strategy for augmenting the power of coordinate-targeted super-resolution microscopy."],["dc.identifier.doi","10.1038/NPHOTON.2015.266"],["dc.identifier.gro","3141737"],["dc.identifier.isi","000369321400015"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/513"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: European Union [PIEF-GA-2011-299283]; Korber Foundation"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1749-4893"],["dc.relation.issn","1749-4885"],["dc.title","Coordinate-targeted fluorescence nanoscopy with multiple off states"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","103"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nano Letters"],["dc.bibliographiccitation.lastpage","106"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Testa, Ilaria"],["dc.contributor.author","D'Este, E."],["dc.contributor.author","Urban, Nicolai T."],["dc.contributor.author","Balzarotti, Francisco"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:44:46Z"],["dc.date.available","2017-09-07T11:44:46Z"],["dc.date.issued","2015"],["dc.description.abstract","We show that RESOLFT fluorescence nanoscopy, a low light level scanning superresolution technique employing reversibly switchable fluorescent proteins (rsFPs), is capable of dual-channel live-cell imaging that is virtually free of chromatic errors and temporal offsets. This is accomplished using rsEGFP and Dronpa, two rsFPs having similar spectra but different kinetics of switching and fluorescence emission. Our approach is demonstrated by imaging protein distributions and dynamics in living neurons and neuronal tissues."],["dc.identifier.doi","10.1021/nl503058k"],["dc.identifier.gro","3141985"],["dc.identifier.isi","000348086100017"],["dc.identifier.pmid","25423166"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/3268"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1530-6992"],["dc.relation.issn","1530-6984"],["dc.title","Dual Channel RESOLFT Nanoscopy by Using Fluorescent State Kinetics"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","577"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.journal","Nature communications"],["dc.bibliographiccitation.lastpage","9"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Richardson, Douglas S."],["dc.contributor.author","Gregor, Carola"],["dc.contributor.author","Winter, Franziska R."],["dc.contributor.author","Urban, Nicolai T."],["dc.contributor.author","Sahl, Steffen J."],["dc.contributor.author","Willig, Katrin I."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2018-01-17T13:31:10Z"],["dc.date.available","2018-01-17T13:31:10Z"],["dc.date.issued","2017"],["dc.description.abstract","Fluorescence-based biosensors have become essential tools for modern biology, allowing real-time monitoring of biological processes within living cells. Intracellular fluorescent pH probes comprise one of the most widely used families of biosensors in microscopy. One key application of pH probes has been to monitor the acidification of vesicles during endocytosis, an essential function that aids in cargo sorting and degradation. Prior to the development of super-resolution fluorescence microscopy (nanoscopy), investigation of endosomal dynamics in live cells remained difficult as these structures lie at or below the ~250 nm diffraction limit of light microscopy. Therefore, to aid in investigations of pH dynamics during endocytosis at the nanoscale, we have specifically designed a family of ratiometric endosomal pH probes for use in live-cell STED nanoscopy.Ratiometric fluorescent pH probes are useful tools to monitor acidification of vesicles during endocytosis, but the size of vesicles is below the diffraction limit. Here the authors develop a family of ratiometric pH sensors for use in STED super-resolution microscopy, and optimize their delivery to endosomes."],["dc.identifier.doi","10.1038/s41467-017-00606-4"],["dc.identifier.pmid","28924139"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16496"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11717"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation.eissn","2041-1723"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","SRpHi ratiometric pH biosensors for super-resolution microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","A"],["dc.bibliographiccitation.journal","ACS Photonics"],["dc.bibliographiccitation.lastpage","J"],["dc.bibliographiccitation.volumetitle","Recent Developments and Applications of Plasmonics"],["dc.contributor.author","Urban, Nicolai T."],["dc.contributor.author","Foreman, Matthew"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Sivan, Yonatan"],["dc.date.accessioned","2018-01-17T13:29:15Z"],["dc.date.available","2018-01-17T13:29:15Z"],["dc.date.issued","2017"],["dc.description.abstract","We demonstrate stimulated emission depletion (STED) microscopy with 20 nm gold nanospheres coated by fluorescently doped silica. We demonstrate significantly improved spatial resolution down to 75 nm, which is the first time that hybrid NPs are used in STED imaging beyond the diffraction limit of confocal microscopy. Unlike previous demonstrations of super-resolution with metal nanoparticles with different techniques, this 3.3-fold resolution improvement was limited only by the particle size. The STED intensity required for this is almost twice lower than for conventional STED based on dye alone, and we observe no melting or displacement of the NPs at the utilized intensities. Moreover, we show that the nanoparticles can be imaged in an aqueous environment, demonstrating the relevance to bioimaging. Finally, we also show, for the first time in this context, an up to 3-fold reduction in the rate of photobleaching compared to standard dye-based STED, thus enabling sustainably brighter images."],["dc.identifier.doi","10.1021/acsphotonics.7b00833"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11714"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.subject","fluorescence microscopy; nanoparticles; nanotechnology; plasmonics; STED nanoscopy; super-resolution"],["dc.title","Nanoparticle-Assisted STED Nanoscopy with Gold Nanospheres"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","186a"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","187a"],["dc.bibliographiccitation.volume","112"],["dc.contributor.author","Danzl, Johann G."],["dc.contributor.author","Sidenstein, Sven"],["dc.contributor.author","Gregor, Carola"],["dc.contributor.author","Urban, Nicolai"],["dc.contributor.author","Ilgen, Peter"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2022-03-01T11:44:58Z"],["dc.date.available","2022-03-01T11:44:58Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1016/j.bpj.2016.11.1034"],["dc.identifier.pii","S0006349516320641"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103178"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.issn","0006-3495"],["dc.title","Coordinate-Targeted Fluorescence Nanoscopy with Multiple Off-States"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]