Busse, Ann-Christin

[["dc.bibliographiccitation.firstpage","420"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Cytometry Part A"],["dc.bibliographiccitation.lastpage","428"],["dc.bibliographiccitation.volume","77A"],["dc.contributor.author","Wessels, Johannes Theodor"],["dc.contributor.author","Busse, Ann-Christin"],["dc.contributor.author","Mahrt, Jens"],["dc.contributor.author","Hoffschulte, B."],["dc.contributor.author","Mueller, Georg Anton"],["dc.contributor.author","Tarnok, A."],["dc.contributor.author","Mittag, A."],["dc.date.accessioned","2018-11-07T08:43:29Z"],["dc.date.available","2018-11-07T08:43:29Z"],["dc.date.issued","2010"],["dc.description.abstract","In recent years, slide-based cytometry has become a key technology for polychromatic cytometric investigations, and many efforts have been made to increase the number of measurable fluorochromes for multiparametric analysis. Sequential photobleaching of fluorochromes next to very photostable dyes is one approach for this technology. As the ALEXA dyes are known to be photostable as compared to the conventional fluorochromes FITC, PE (Riggs et al., Am J Pathol 1958;34:1081-1097), and APC, a differentiation within a fluorochrome pair is possible. Here, we have analyzed the newly available NorthernLights (TM) secondary antibodies for use in slide-based cytometry and microscopy. Currently, these fluorochrome-conjugates are now available with three distinct excitation- and emission maxima (NL493, NL557, NL637). Their spectral properties are similar to the frequently used fluorochromes FITC, PE, and APC and can, therefore, be used with most common excitation sources of cytometers or microscopes. As the NorthernLights (TM) are bright, resistant to photobleaching, stable in alcohols and xylene and of affordable price, these dyes are promising candidates for use with most laser- and HBO/XBA-based fluorescence microscopy-like techniques. (C) 2010 International Society for Advancement of Cytometry"],["dc.identifier.doi","10.1002/cyto.a.20863"],["dc.identifier.isi","000277174000003"],["dc.identifier.pmid","20151452"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/19976"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-liss"],["dc.publisher.place","Hoboken"],["dc.relation.conference","25th Congress of the International-Society-for-Advancement-of-Cytometry"],["dc.relation.eventlocation","Seattle, WA"],["dc.relation.issn","1552-4922"],["dc.title","NorthernLights in Slide-Based Cytometry and Microscopy"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","750"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Cytometry Part A"],["dc.bibliographiccitation.lastpage","751"],["dc.bibliographiccitation.volume","71A"],["dc.contributor.author","Wessels, Johannes Theodor"],["dc.contributor.author","Busse, Ann-Christin"],["dc.contributor.author","Tepper-Wessels, Kathrin"],["dc.contributor.author","Mahrt, Jens"],["dc.contributor.author","Hoffschulte, B."],["dc.contributor.author","Grabbe, Eckhard"],["dc.contributor.author","Mueller, Georg Anton"],["dc.date.accessioned","2018-11-07T10:58:51Z"],["dc.date.available","2018-11-07T10:58:51Z"],["dc.date.issued","2007"],["dc.identifier.isi","000249187800036"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/50561"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-liss"],["dc.publisher.place","Hoboken"],["dc.relation.conference","17th Annual Meeting of the German-Society-of-Cytometry (DGfZ)"],["dc.relation.eventlocation","Univ Hosp, Regensburg, Regensburg, GERMANY"],["dc.relation.issn","1552-4922"],["dc.title","Molecular imaging in vivo - A comparative overview"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","365"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Cell Proliferation"],["dc.bibliographiccitation.lastpage","374"],["dc.bibliographiccitation.volume","41"],["dc.contributor.author","Seitz, G."],["dc.contributor.author","Warmann, S. W."],["dc.contributor.author","Fuchs, J."],["dc.contributor.author","Heitmann, H."],["dc.contributor.author","Mahrt, J."],["dc.contributor.author","Busse, A.-C."],["dc.contributor.author","Ruck, P."],["dc.contributor.author","Hoffman, R. M."],["dc.contributor.author","Wessels, J. T."],["dc.date.accessioned","2018-11-07T11:16:23Z"],["dc.date.available","2018-11-07T11:16:23Z"],["dc.date.issued","2008"],["dc.description.abstract","Objective: The aim of this study was to establish a preclinical mouse model to study metastases of paediatric rhabdomyosarcoma at the macroscopic and cellular levels, with different imaging methods. Experimental Design: The alveolar rhabdomyosarcoma cell line Rh30 was stably transfected with the red fluorescent protein (DsRed2) then was xenotransplanted (intravenous injection [n = 8], and footpad injection [n = 8]) into nude mice (NMRI nu/nu). Macroscopic imaging of metastases was performed using DsRed2-fluorescence and flat-panel volumetric computed tomography scan. In a further series of animals (n = 8), in vivo cell trafficking of rhabdomyosarcoma cells using cellular imaging with an Olympus OV100 variable-magnification small-animal imaging system was used. Results: Metastases in the pelvis, thoracic wall and skin were visualized by fluorescence imaging. Pelvic metastases were found after tail vein injection and at other metastatic sites after footpad injection. Flat-panel volumetric computed tomography scan data allowed highly specific analysis of contrast between tumour and surrounding tissue. Correlation between fluorescence and flat-panel volumetric computed tomography scan imaging data was observed. Single-cell imaging visualized tumour cells in the vessels and demonstrated the arrest of tumour cells at vessel junctions followed by extravasation of the tumour cells. Conclusion: We established a model for visualization of experimental metastatic invasion and describe relevant tools for imaging childhood rhabdomyosarcoma metastases at the macroscopic and cellular levels. Imaging of cell trafficking visualized the behaviour of tumour cells and development of metastases by accumulation and extravasation of rhabdomyosarcoma cells."],["dc.identifier.doi","10.1111/j.1365-2184.2008.00520.x"],["dc.identifier.isi","000253978100013"],["dc.identifier.pmid","18336479"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/54573"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.notes.submitter","Najko"],["dc.relation.issn","0960-7722"],["dc.title","Imaging of cell trafficking and metastases of paediatric rhabdomyosarcoma"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","228"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Photochemistry and Photobiology"],["dc.bibliographiccitation.lastpage","235"],["dc.bibliographiccitation.volume","84"],["dc.contributor.author","Wessels, Johannes Theodor"],["dc.contributor.author","Busse, Ann-Christin"],["dc.contributor.author","Rave-Fraenk, Margret"],["dc.contributor.author","Zaenker, Stephan"],["dc.contributor.author","Hermann, Robert"],["dc.contributor.author","Grabbe, Eckhardt"],["dc.contributor.author","Mueller, Gerhard-Anton"],["dc.date.accessioned","2018-11-07T11:20:35Z"],["dc.date.available","2018-11-07T11:20:35Z"],["dc.date.issued","2008"],["dc.description.abstract","The renal cell carcinoma (RCC) is extremely resistant to chemotherapy and radiotherapy. The prognosis of patients with metastatic RCC still remains poor, the median survival is less than 12 months. Therefore, new therapeutic options are desirable. The aim of this study was to investigate the photosensitizing and radiosensitizing effects of hypericin on human RCC cells in vitro. First the RCC-derived cell lines A498 and ACHN were incubated with different concentrations of hypericin. In vitro uptake and intracellular distribution of hypericin were confirmed by fluorescence microscopy. Subsequently cells were illuminated and irradiated with a dose of 2-8 Gy, respectively. Finally, metabolic activity, apoptosis and clonogenic survival were investigated. Uptake of hypericin was observed for almost all cells. Hypericin treatment combined with illumination led to a 94-97% decrease in metabolic activity and caused apoptosis in nearly 100% of RCC cells. Hypericin enhanced the radiosensitivity of A498 cells in vitro. The clonogenic survival after irradiation was significantly reduced by hypericin treatment. Taken together, the photosensitizing and radiosensitizing effects of hypericin on human RCC cells we found in this investigation could be of clinical relevance, e.g. for radiotherapy and intraoperative photodynamic therapy, respectively."],["dc.identifier.doi","10.1111/j.1751-1097.2007.00225.x"],["dc.identifier.isi","000252506900032"],["dc.identifier.pmid","18173725"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/55568"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Photobiology"],["dc.relation.issn","0031-8655"],["dc.title","Photosensitizing and radiosensitizing effects of hypericin on human renal carcinoma cells in Vitro"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Nephrology Dialysis Transplantation"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Wessels, Johannes"],["dc.contributor.author","Busse, Ann-Christin"],["dc.contributor.author","Mahrt, Jens"],["dc.contributor.author","Hoffman, Robert M."],["dc.contributor.author","Mueller, Gerhard-Anton"],["dc.date.accessioned","2018-11-07T11:07:09Z"],["dc.date.available","2018-11-07T11:07:09Z"],["dc.date.issued","2007"],["dc.format.extent","233"],["dc.identifier.isi","000253320600644"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52485"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.publisher.place","Oxford"],["dc.relation.conference","44th ERA-EDTA Congress"],["dc.relation.eventlocation","Barcelona, SPAIN"],["dc.relation.issn","0931-0509"],["dc.title","Real-time subcellular imaging of renal cell carcinoma-cell trafficking in live mice"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","S1158"],["dc.bibliographiccitation.journal","Annals of Oncology"],["dc.bibliographiccitation.volume","32"],["dc.contributor.author","Büntzel, J."],["dc.contributor.author","Micke, O."],["dc.contributor.author","Büssing, A."],["dc.contributor.author","Büntzel, J."],["dc.date.accessioned","2021-12-01T09:23:31Z"],["dc.date.available","2021-12-01T09:23:31Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1016/j.annonc.2021.08.1629"],["dc.identifier.pii","S0923753421038588"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/94674"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-478"],["dc.relation.issn","0923-7534"],["dc.rights.uri","https://www.elsevier.com/tdm/userlicense/1.0/"],["dc.title","1636P Health behavior of cancer patients during COVID-19 pandemic. Focus head neck cancer"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","25"],["dc.bibliographiccitation.journal","Physical Review Letters"],["dc.bibliographiccitation.volume","100"],["dc.contributor.author","Arnèodo, A."],["dc.contributor.author","Benzi, R."],["dc.contributor.author","Berg, J."],["dc.contributor.author","Biferale, L."],["dc.contributor.author","Bodenschatz, E."],["dc.contributor.author","Busse, A."],["dc.contributor.author","Calzavarini, E."],["dc.contributor.author","Castaing, B."],["dc.contributor.author","Cencini, M."],["dc.contributor.author","Chevillard, L."],["dc.contributor.author","Yeung, P. K."],["dc.date.accessioned","2022-06-08T07:59:42Z"],["dc.date.available","2022-06-08T07:59:42Z"],["dc.date.issued","2008"],["dc.identifier.doi","10.1103/PhysRevLett.100.254504"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/110836"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-575"],["dc.relation.eissn","1079-7114"],["dc.relation.issn","0031-9007"],["dc.title","Universal Intermittent Properties of Particle Trajectories in Highly Turbulent Flows"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","123"],["dc.bibliographiccitation.journal","Onkologie"],["dc.bibliographiccitation.lastpage","124"],["dc.bibliographiccitation.volume","33"],["dc.contributor.author","Laffers, Wiebke"],["dc.contributor.author","Busse, A.-C."],["dc.contributor.author","Mahrt, Jens"],["dc.contributor.author","Gerstner, Andreas O. H."],["dc.contributor.author","Wessels, Johannes Theodor"],["dc.date.accessioned","2018-11-07T08:46:30Z"],["dc.date.available","2018-11-07T08:46:30Z"],["dc.date.issued","2010"],["dc.identifier.isi","000274745200344"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/20707"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Karger"],["dc.publisher.place","Basel"],["dc.title","Photodynamic effects of hypericin on head and neck carcinoma in vitro and in vivo"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","711"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","European Archives of Oto-Rhino-Laryngology"],["dc.bibliographiccitation.lastpage","718"],["dc.bibliographiccitation.volume","272"],["dc.contributor.author","Laffers, Wiebke"],["dc.contributor.author","Busse, Ann-Christin"],["dc.contributor.author","Mahrt, Jens"],["dc.contributor.author","Phuc Nguyen, Phuc Nguyen"],["dc.contributor.author","Gerstner, Andreas O. H."],["dc.contributor.author","Bootz, Friedrich"],["dc.contributor.author","Wessels, Johannes Theodor"],["dc.date.accessioned","2018-11-07T10:00:38Z"],["dc.date.available","2018-11-07T10:00:38Z"],["dc.date.issued","2015"],["dc.description.abstract","Clinical outcome of patients suffering from head neck squamous cell carcinomas is still poor due to recurrent disease and surgical limitations. There is still a demand for multimodality approaches and new therapeutic options. Hypericin is a promising phototoxic drug which was investigated for its effects on head neck squamous cell carcinoma cells in vitro. FaDu cells incubated with or without hypericin were illuminated (450-700 nm, 50,000 lx) for different time periods. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide- and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay were used to score metabolic and apoptotic activity. Even after the shortest illumination FaDu cells incubated with hypericin showed massive reduction of metabolism and excessive apoptosis. This was present even with the lowest hypericin concentration. Cells without hypericin or without illumination were not affected. These photosensitizing effects of hypericin could be suitable for clinical application and could lead to the development of an intraoperative photodynamic therapy of head neck squamous cell carcinomas."],["dc.identifier.doi","10.1007/s00405-014-2984-8"],["dc.identifier.isi","000350031400027"],["dc.identifier.pmid","24687800"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/37852"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1434-4726"],["dc.relation.issn","0937-4477"],["dc.title","Photosensitizing effects of hypericin on head neck squamous cell carcinoma in vitro"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","238"],["dc.bibliographiccitation.journal","Nephrology Dialysis Transplantation"],["dc.bibliographiccitation.lastpage","239"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Wessels, Johannes"],["dc.contributor.author","Busse, Ann-Christin"],["dc.contributor.author","Rave-Fraenk, Margret"],["dc.contributor.author","Zaenker, Stephan"],["dc.contributor.author","Grabbe, Eckhard"],["dc.contributor.author","Mueller, Gerhard-Anton"],["dc.date.accessioned","2018-11-07T11:07:09Z"],["dc.date.available","2018-11-07T11:07:09Z"],["dc.date.issued","2007"],["dc.identifier.isi","000253320600660"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52486"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.publisher.place","Oxford"],["dc.relation.conference","44th ERA-EDTA Congress"],["dc.relation.eventlocation","Barcelona, SPAIN"],["dc.relation.issn","0931-0509"],["dc.title","Photo and radiosensitizing effects of hypericin on human renal cell carcinoma cells in vitro"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]