Darienko, Tatyana M.

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Pröschold, Thomas"],["dc.contributor.author","Rieser, Daniel"],["dc.contributor.author","Darienko, Tatyana"],["dc.contributor.author","Nachbaur, Laura"],["dc.contributor.author","Kammerlander, Barbara"],["dc.contributor.author","Qian, Kuimei"],["dc.contributor.author","Pitsch, Gianna"],["dc.contributor.author","Bruni, Estelle Patricia"],["dc.contributor.author","Qu, Zhishuai"],["dc.contributor.author","Forster, Dominik"],["dc.contributor.author","Rad-Menendez, Cecilia"],["dc.contributor.author","Posch, Thomas"],["dc.contributor.author","Stoeck, Thorsten"],["dc.contributor.author","Sonntag, Bettina"],["dc.date.accessioned","2021-04-14T08:28:35Z"],["dc.date.available","2021-04-14T08:28:35Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1038/s41598-021-84265-y"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82654"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","2045-2322"],["dc.title","An integrative approach sheds new light onto the systematics and ecology of the widespread ciliate genus Coleps (Ciliophora, Prostomatea)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","921"],["dc.bibliographiccitation.journal","Frontiers in Physiology"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Bajerski, Felizitas"],["dc.contributor.author","Stock, Johanna"],["dc.contributor.author","Hanf, Benjamin"],["dc.contributor.author","Darienko, Tatyana"],["dc.contributor.author","Heine-Dobbernack, Elke"],["dc.contributor.author","Lorenz, Maike"],["dc.contributor.author","Naujox, Lisa"],["dc.contributor.author","Keller, E. R. J."],["dc.contributor.author","Schumacher, H. M."],["dc.contributor.author","Friedl, Thomas"],["dc.contributor.author","Eberth, Sonja"],["dc.contributor.author","Mock, Hans-Peter"],["dc.contributor.author","Kniemeyer, Olaf"],["dc.contributor.author","Overmann, Jörg"],["dc.date.accessioned","2019-07-22T14:46:42Z"],["dc.date.available","2019-07-22T14:46:42Z"],["dc.date.issued","2018"],["dc.description.abstract","In many natural environments, organisms get exposed to low temperature and/or to strong temperature shifts. Also, standard preservation protocols for live cells or tissues involve ultradeep freezing in or above liquid nitrogen (-196°C or -150°C, respectively). To which extent these conditions cause cold- or cryostress has rarely been investigated systematically. Using ATP content as an indicator of the physiological state of cells, we found that representatives of bacteria, fungi, algae, plant tissue, as well as plant and human cell lines exhibited similar responses during freezing and thawing. Compared to optimum growth conditions, the cellular ATP content of most model organisms decreased significantly upon treatment with cryoprotectant and cooling to up to -196°C. After thawing and a longer period of regeneration, the initial ATP content was restored or even exceeded the initial ATP levels. To assess the implications of cellular ATP concentration for the physiology of cryostress, cell viability was determined in parallel using independent approaches. A significantly positive correlation of ATP content and viability was detected only in the cryosensitive algae Chlamydomonas reinhardtii SAG 11-32b and Chlorella variabilis NC64A, and in plant cell lines of Solanum tuberosum. When comparing mesophilic with psychrophilic bacteria of the same genera, and cryosensitive with cryotolerant algae, ATP levels of actively growing cells were generally higher in the psychrophilic and cryotolerant representatives. During exposure to ultralow temperatures, however, psychrophilic and cryotolerant species showed a decline in ATP content similar to their mesophilic or cryosensitive counterparts. Nevertheless, psychrophilic and cryotolerant species attained better culturability after freezing. Cellular ATP concentrations and viability measurements thus monitor different features of live cells during their exposure to ultralow temperatures and cryostress."],["dc.identifier.doi","10.3389/fphys.2018.00921"],["dc.identifier.pmid","30065659"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61835"],["dc.language.iso","en"],["dc.notes.intern","DeepGreen Import"],["dc.publisher","Frontiers Media S.A."],["dc.relation.eissn","1664-042X"],["dc.relation.issn","1664-042X"],["dc.rights","http://creativecommons.org/licenses/by/4.0/"],["dc.title","ATP Content and Cell Viability as Indicators for Cryostress Across the Diversity of Life"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","229"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Microbial Ecology"],["dc.bibliographiccitation.lastpage","247"],["dc.bibliographiccitation.volume","57"],["dc.contributor.author","Buedel, Burkhard"],["dc.contributor.author","Darienko, Tatyana"],["dc.contributor.author","Deutschewitz, Kirstin"],["dc.contributor.author","Dojani, Stephanie"],["dc.contributor.author","Friedl, Thomas"],["dc.contributor.author","Mohr, Kathrin I."],["dc.contributor.author","Salisch, Mario"],["dc.contributor.author","Reisser, Werner"],["dc.contributor.author","Weber, Bettina"],["dc.date.accessioned","2018-11-07T08:33:14Z"],["dc.date.available","2018-11-07T08:33:14Z"],["dc.date.issued","2009"],["dc.description.abstract","Biological soil crusts (BSCs) are found in all dryland regions of the world, including the polar regions. They are also known to occur in the southern African region. Although there were a number of case studies on BSCs from that region, we did not know if they are a normal part of the vegetation cover or just a phenomenon that occasionally occurs here and there. In order to investigate diversity, distribution patterns, and the driving factors of both, we followed a random sampling system of observatories along a transect, stretching from the Namibian-Angolan border down south to the Cape Peninsula, covering seven different major biomes. Biological soil crusts were found to occur in six out of seven biomes. Despite the fact that soil-dwelling algae occurred in the Fynbos biome, crust formation was not observed for hitherto unknown reasons. Seven BSC types were distinguished on the basis of morphology and taxonomic composition: three of them were cyanobacteria-dominated, one with additional chlorolichens, two with bryophytes, one hypolithic type restricted to quartz gravel pavements, and the unique lichen fields of the Namib Desert. Besides 29 green algal species in 21 genera, one heterokont alga, 12 cyanolichens, 14 chlorolichens, two genera of liverworts, and three genera of mosses, these crusts are positioned among the most diverse BSCs worldwide mainly because of the unusual high cyanobacterial species richness comprising 58 species in 21 genera. They contribute considerably to the biodiversity of arid and semi-arid bioregions. Taxonomic diversity of cyanobacteria was significantly higher in the winter rain zone than in the summer rain zone (54 versus 32 species). The soil photosynthetic biomass (chlorophyll(a)/m(2)), the carbon content of the soil and the number of BSC types were significantly higher in the winter rain zone (U (27, 29) = 215.0, p = 0.004 [chl(a)]; U (21, 21) = 135.0, p = 0.031 [C]; U (27, 29) = 261.5, p = 0.028 [BSC types]; excluding the fog-dominated Namib biome). The winter rain zone is characterized by a lower precipitation amount, but a higher rain frequency with the number of rainy days more evenly distributed over the year. The dry period is significantly shorter per year in the winter rain zone (U (8, 9) = 5.0, p = 0.003). We conclude that rain frequency and duration of dry periods rather than the precipitation amount is the main factor for BSC growth and succession. Nitrogen content of the soils along the transect was generally very low and correlated with soil carbon content. There was a weak trend that an increasing proportion of silt and clay (< 0.63 mm) in the soil is associated with higher values of BSC chlorophyll content (Pearson correlation coefficient = 0.314, p = 0.237). Furthermore, we found a significant positive correlation between silt and clay and the number of BSC types (Pearson correlation coefficient = 0.519, p = 0.039), suggesting that fine grain-size promotes BSC succession and their biomass content. Lichens and bryophytes occurred in BSCs with lower disturbance frequencies (e.g. trampling) only. Crust thickness and chlorophyll content increased significantly from crusts of the early successional type to the late successional crust types. From our results, we conclude that BSCs are a normal and frequent element of the vegetation in arid and semi-arid southwestern Africa, and that rain frequency and duration of dry periods rather than the precipitation amount are the key factors for the development, differentiation and compsition of BSCs."],["dc.description.sponsorship","German Ministry of Education and Research (BMBF)"],["dc.identifier.doi","10.1007/s00248-008-9449-9"],["dc.identifier.isi","000262829500003"],["dc.identifier.pmid","18850242"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17527"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0095-3628"],["dc.title","Southern African Biological Soil Crusts are Ubiquitous and Highly Diverse in Drylands, Being Restricted by Rainfall Frequency"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","573"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Extremophiles"],["dc.bibliographiccitation.lastpage","586"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Darienko, Tatyana"],["dc.contributor.author","Kang, Woojean"],["dc.contributor.author","Orzechowski, Aleksander K."],["dc.contributor.author","Pröschold, Thomas"],["dc.date.accessioned","2020-12-10T14:11:06Z"],["dc.date.available","2020-12-10T14:11:06Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1007/s00792-019-01108-5"],["dc.identifier.eissn","1433-4909"],["dc.identifier.issn","1431-0651"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16695"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/70965"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Pleurastrosarcina terriformae, a new species of a rare desert trebouxiophycean alga discovered by an integrative approach"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","811"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Systematics and Biodiversity"],["dc.bibliographiccitation.lastpage","829"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Darienko, Tatyana"],["dc.contributor.author","Menéndez, Cecilia Rad"],["dc.contributor.author","Campbell, Christine"],["dc.contributor.author","Pröschold, Thomas"],["dc.date.accessioned","2020-04-03T13:03:31Z"],["dc.date.available","2020-04-03T13:03:31Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1080/14772000.2019.1690597"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63608"],["dc.relation.issn","1477-2000"],["dc.relation.issn","1478-0933"],["dc.title","Are there any true marine Chlorella species? Molecular phylogenetic assessment and ecology of marine Chlorella-like organisms, including a description of Droopiella gen. nov"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","204"],["dc.bibliographiccitation.journal","European Journal of Phycology"],["dc.bibliographiccitation.lastpage","205"],["dc.bibliographiccitation.volume","50"],["dc.contributor.author","Darienko, Tatyana"],["dc.contributor.author","Siegesmund, Maria A."],["dc.contributor.author","Lorenz, Maike"],["dc.contributor.author","Rybalka, Nataliya"],["dc.contributor.author","Friedl, Thomas"],["dc.date.accessioned","2018-11-07T10:03:01Z"],["dc.date.available","2018-11-07T10:03:01Z"],["dc.date.issued","2015"],["dc.identifier.isi","000360244400382"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38353"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Taylor & Francis Ltd"],["dc.publisher.place","Abingdon"],["dc.relation.issn","1469-4433"],["dc.relation.issn","0967-0262"],["dc.title","EFFECTS OF CRYOPRESERVATION ON SELECTED GREEN MICROALGAE USING AFLP FINGERPRINTING FOR GENOMIC AND EPIGENOMIC STABILITY ASSESSMENT"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1963"],["dc.bibliographiccitation.journal","Proceedings of the Royal Society of London. Series B, Biological Sciences"],["dc.bibliographiccitation.volume","288"],["dc.contributor.author","Irisarri, Iker"],["dc.contributor.author","Darienko, Tatyana M."],["dc.contributor.author","Pröschold, Thomas"],["dc.contributor.author","Fürst-Jansen, Janine M. R."],["dc.contributor.author","Jamy, Mahwash"],["dc.contributor.author","de Vries, Jan"],["dc.date.accessioned","2021-12-01T09:21:07Z"],["dc.date.available","2021-12-01T09:21:07Z"],["dc.date.issued","2021"],["dc.description.abstract","Streptophytes are one of the major groups of the green lineage (Chloroplastida or Viridiplantae). During one billion years of evolution, streptophytes have radiated into an astounding diversity of uni- and multicellular green algae as well as land plants. Most divergent from land plants is a clade formed by Mesostigmatophyceae, Spirotaenia spp. and Chlorokybophyceae. All three lineages are species-poor and the Chlorokybophyceae consist of a single described species, Chlorokybus atmophyticus. In this study, we used phylogenomic analyses to shed light into the diversity within Chlorokybus using a sampling of isolates across its known distribution. We uncovered a consistent deep genetic structure within the Chlorokybus isolates, which prompted us to formally extend the Chlorokybophyceae by describing four new species. Gene expression differences among Chlorokybus species suggest certain constitutive variability that might influence their response to environmental factors. Failure to account for this diversity can hamper comparative genomic studies aiming to understand the evolution of stress response across streptophytes. Our data highlight that future studies on the evolution of plant form and function can tap into an unknown diversity at key deep branches of the streptophytes."],["dc.description.abstract","Streptophytes are one of the major groups of the green lineage (Chloroplastida or Viridiplantae). During one billion years of evolution, streptophytes have radiated into an astounding diversity of uni- and multicellular green algae as well as land plants. Most divergent from land plants is a clade formed by Mesostigmatophyceae, Spirotaenia spp. and Chlorokybophyceae. All three lineages are species-poor and the Chlorokybophyceae consist of a single described species, Chlorokybus atmophyticus. In this study, we used phylogenomic analyses to shed light into the diversity within Chlorokybus using a sampling of isolates across its known distribution. We uncovered a consistent deep genetic structure within the Chlorokybus isolates, which prompted us to formally extend the Chlorokybophyceae by describing four new species. Gene expression differences among Chlorokybus species suggest certain constitutive variability that might influence their response to environmental factors. Failure to account for this diversity can hamper comparative genomic studies aiming to understand the evolution of stress response across streptophytes. Our data highlight that future studies on the evolution of plant form and function can tap into an unknown diversity at key deep branches of the streptophytes."],["dc.identifier.doi","10.1098/rspb.2021.2168"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/94351"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-478"],["dc.relation.eissn","1471-2954"],["dc.relation.issn","0962-8452"],["dc.title","Unexpected cryptic species among streptophyte algae most distant to land plants"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Spanner, Christian"],["dc.contributor.author","Darienko, Tatyana"],["dc.contributor.author","Filker, Sabine"],["dc.contributor.author","Sonntag, Bettina"],["dc.contributor.author","Pröschold, Thomas"],["dc.date.accessioned","2022-12-01T08:30:55Z"],["dc.date.available","2022-12-01T08:30:55Z"],["dc.date.issued","2022"],["dc.description.abstract","Abstract\r\n \r\n Paramecium bursaria\r\n is a mixotrophic ciliate species, which is common in stagnant and slow-flowing, nutrient-rich waters. It is usually found living in symbiosis with\r\n zoochlorellae\r\n (green algae) of the genera\r\n Chlorella\r\n or\r\n Micractinium\r\n . We investigated\r\n P. bursaria\r\n isolates from around the world, some of which have already been extensively studied in various laboratories, but whose morphological and genetic identity has not yet been completely clarified. Phylogenetic analyses of the SSU and ITS rDNA sequences revealed five highly supported lineages, which corresponded to the syngen and most likely to the biological species assignment. These syngens R1–R5 could also be distinguished by unique synapomorphies in the secondary structures of the SSU and the ITS. Considering these synapomorphies, we could clearly assign the existing GenBank entries of\r\n P. bursaria\r\n to specific syngens. In addition, we discovered synapomorphies at amino acids of the COI gene for the identification of the syngens. Using the metadata of these entries, most syngens showed a worldwide distribution, however, the syngens R1 and R5 were only found in Europe. From morphology, the syngens did not show any significant deviations. The investigated strains had either\r\n Chlorella variabilis\r\n ,\r\n Chlorella vulgaris\r\n or\r\n Micractinium conductrix\r\n as endosymbionts."],["dc.identifier.doi","10.1038/s41598-022-22284-z"],["dc.identifier.pii","22284"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/118019"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-621"],["dc.relation.eissn","2045-2322"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Morphological diversity and molecular phylogeny of five Paramecium bursaria (Alveolata, Ciliophora, Oligohymenophorea) syngens and the identification of their green algal endosymbionts"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2327"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Journal of Applied Phycology"],["dc.bibliographiccitation.lastpage","2340"],["dc.bibliographiccitation.volume","33"],["dc.contributor.author","Reichelt, Niklas"],["dc.contributor.author","Leger, Dorian"],["dc.contributor.author","Daubert, Mareike"],["dc.contributor.author","Ruffino, Paolo"],["dc.contributor.author","Pröschold, Thomas"],["dc.contributor.author","Darienko, Tatyana"],["dc.date.accessioned","2021-08-12T07:46:13Z"],["dc.date.available","2021-08-12T07:46:13Z"],["dc.date.issued","2021"],["dc.description.abstract","Abstract Chromochloris zofingiensis (Dönz) Fucíková & L.A.Lewis, due to its production of highly valuable carotenoids such as astaxanthin, is a model organism in biotechnology. Since the recognition of this physiological property, many biotechnological applications have only used a single strain (SAG 211-14 = CCAP 211/14 = UTEX 32 = ATCC 30412) to produce biomass and carotenoids. However, multiple acquisitions of strains putatively belonging to the same species raised the question of the conspecificity of those strains and their properties. In this study, the conspecificity of the available strains, which are deposited axenically in SAG, was tested using SSU and ITS rDNA sequencing and AFLP ( Eco RI/ Pst I) analyses. The comparison of SSU and ITS rDNA sequences as well as the AFLP patterns revealed that the investigated strains formed two very similar groups, (1) SAG 211-14, SAG 4.80, SAG 31.80, and SAG 34.80 and (2) SAG 221-2. All strains belonged to one species, C. zofingiensis , and represented one monophyletic lineage within the so-called DO-group of the Chlorophyceae. The robustness to cryopreservation and the subsequent epigenetic variability was detected using the methylation-sensitive AFLP ( Eco RI/ Msp I and Eco RI/ Hpa II) among the five Chromochloris strains. All strains showed a high rate of survival (54.4–98.1%) during cryopreservation. The methylation patterns varied between precryo and postcryo in all strains detected among three time points (before, shortly after, and 8 weeks after cryopreservation), showing that the MS-AFLP technique has the potential to detect epigenetic effects occurring in response to cryopreservation and other stresses. Finally, the potential of these five strains for usage in biotechnological applications was proven by growing them in aerated cultures with and without additional carbon dioxide supply. The comparison showed that all strains produced high amounts of biomass and carotenoids under aeration with additional CO 2 and were therefore suitable in biotechnology."],["dc.description.abstract","Abstract Chromochloris zofingiensis (Dönz) Fucíková & L.A.Lewis, due to its production of highly valuable carotenoids such as astaxanthin, is a model organism in biotechnology. Since the recognition of this physiological property, many biotechnological applications have only used a single strain (SAG 211-14 = CCAP 211/14 = UTEX 32 = ATCC 30412) to produce biomass and carotenoids. However, multiple acquisitions of strains putatively belonging to the same species raised the question of the conspecificity of those strains and their properties. In this study, the conspecificity of the available strains, which are deposited axenically in SAG, was tested using SSU and ITS rDNA sequencing and AFLP ( Eco RI/ Pst I) analyses. The comparison of SSU and ITS rDNA sequences as well as the AFLP patterns revealed that the investigated strains formed two very similar groups, (1) SAG 211-14, SAG 4.80, SAG 31.80, and SAG 34.80 and (2) SAG 221-2. All strains belonged to one species, C. zofingiensis , and represented one monophyletic lineage within the so-called DO-group of the Chlorophyceae. The robustness to cryopreservation and the subsequent epigenetic variability was detected using the methylation-sensitive AFLP ( Eco RI/ Msp I and Eco RI/ Hpa II) among the five Chromochloris strains. All strains showed a high rate of survival (54.4–98.1%) during cryopreservation. The methylation patterns varied between precryo and postcryo in all strains detected among three time points (before, shortly after, and 8 weeks after cryopreservation), showing that the MS-AFLP technique has the potential to detect epigenetic effects occurring in response to cryopreservation and other stresses. Finally, the potential of these five strains for usage in biotechnological applications was proven by growing them in aerated cultures with and without additional carbon dioxide supply. The comparison showed that all strains produced high amounts of biomass and carotenoids under aeration with additional CO 2 and were therefore suitable in biotechnology."],["dc.identifier.doi","10.1007/s10811-021-02468-2"],["dc.identifier.pii","2468"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/88649"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-448"],["dc.relation.eissn","1573-5176"],["dc.relation.issn","0921-8971"],["dc.title","Epigenomic stability assessment during cryopreservation and physiology among various strains of Chromochloris zofingiensis (Chlorophyceae) and their genetic variability revealed by AFLP and MS-AFLP"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1125"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Journal of Phycology"],["dc.bibliographiccitation.lastpage","1145"],["dc.bibliographiccitation.volume","52"],["dc.contributor.author","Darienko, Tatyana"],["dc.contributor.author","Gustavs, Lydia"],["dc.contributor.author","Proeschold, Thomas"],["dc.date.accessioned","2018-11-07T10:05:05Z"],["dc.date.available","2018-11-07T10:05:05Z"],["dc.date.issued","2016"],["dc.description.abstract","The genera Elliptochloris and Pseudochlorella were erected for Chlorella-like green algae producing two types of autospores and cell packages, respectively. Both genera are widely distributed in different soil habitats, either as free living or as photobionts of lichens. The species of these genera are often difficult to identify because of the high phenotypic plasticity and occasional lack of characteristic features. The taxonomic and nomenclatural status of these species, therefore, remains unclear. In this study, 34 strains were investigated using an integrative approach. Phylogenetic analyses demonstrated that the isolates belong to two independent lineages of the Trebouxiophyceae (Elliptochloris and Prasiola clades) and confirmed that the genera are not closely related. The comparison of morphology, molecular phylogeny, and analyses of secondary structures of SSU and ITS rDNA sequences revealed that all of the strains belong to three genera: Elliptochloris, Pseudochlorella, and Edaphochlorella. As a consequence of the taxonomic revisions, we propose two new combinations (Elliptochloris antarctica and Pseudochlorella signiensis) and validate Elliptochloris reniformis, which is invalidly described according to the International Code for Nomenclature (ICN), by designating a holotype. To reflect the high phenotypic plasticity of P. signiensis, two new varieties were described: P. signiensis var. magna and P. signiensis var. communis. Chlorella mirabilis was not closely related to any of these genera and was, therefore, transferred to the new genus Edaphochlorella. All of the taxonomic changes were highly supported by all phylogenetic analyses and were confirmed by the ITS-2 Barcodes using the ITS-2/CBC approach."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft (DFG) [GU 1278/1-1]"],["dc.identifier.doi","10.1111/jpy.12481"],["dc.identifier.isi","000390344600019"],["dc.identifier.pmid","27734501"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38829"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","1529-8817"],["dc.relation.issn","0022-3646"],["dc.title","SPECIES CONCEPT AND NOMENCLATURAL CHANGES WITHIN THE GENERA ELLIPTOCHLORIS AND PSEUDOCHLORELLA (TREBOUXIOPHYCEAE) BASED ON AN INTEGRATIVE APPROACH"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]