Viebahn, Christoph

[["dc.bibliographiccitation.firstpage","127"],["dc.bibliographiccitation.journal","European Journal of Cell Biology"],["dc.bibliographiccitation.lastpage","128"],["dc.bibliographiccitation.volume","82"],["dc.contributor.author","Puschel, B."],["dc.contributor.author","Weckelmann, A."],["dc.contributor.author","Viebahn, Christoph"],["dc.date.accessioned","2018-11-07T10:40:19Z"],["dc.date.available","2018-11-07T10:40:19Z"],["dc.date.issued","2003"],["dc.identifier.isi","000182016200309"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/46277"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Urban & Fischer Verlag"],["dc.publisher.place","Jena"],["dc.relation.conference","Annual Meeting of the Deutschen-Gesellschaft-fur-Zellbiologie/Gesellschaft-fur-Entwicklungsbio logie"],["dc.relation.eventlocation","BONN, GERMANY"],["dc.relation.issn","0171-9335"],["dc.title","Redistribution of the germ cell antigen PG2 during initial stages of embryogenesis"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","31"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Sexual Development"],["dc.bibliographiccitation.lastpage","42"],["dc.bibliographiccitation.volume","2"],["dc.contributor.author","Weckelmann, A."],["dc.contributor.author","Viebahn, Christoph"],["dc.contributor.author","Pueschel, Bernd"],["dc.date.accessioned","2018-11-07T11:20:45Z"],["dc.date.available","2018-11-07T11:20:45Z"],["dc.date.issued","2008"],["dc.description.abstract","Mitochondria are central players in diverse cellular functions and their efficient functioning has dramatic impact on embryonic development. Apparently, proliferation and transmission of well functioning mitochondria to the next generation require ingeniously adapted mechanisms, one of which, the 'mitochondrial bottleneck', is thought to occur early in mammalian development during primordial germ cell ( PGC) specification. We used an antibody directed against the mitochondrial PG2 epitope, a reliable marker of primordial and adult female germ cells to monitor mitochondrial differentiation in the early rabbit embryo. Early development shows the PG2 epitope either tightly mitochondriaassociated ( zygote) or diffusely distributed throughout cytoplasm ( cleavage stages). Mitochondrial colocalization of the PG2 epitope is regained in the early blastocyst but expression is then retained by the hypoblast and epiblast only, with the epiblast, although the forerunner of PGCs, showing weak and diffuse labeling only. At gastrulation, hypoblast cells lose PG2 expression but intensive PG2 labeling is found again on all mitochondria in the first PGCs and reveals the number of mitochondria to be in the range of 50 to 100 per PGC at this stage. The results highlight the dynamics of PG2 expression during early development and the usefulness of the epitope for testing the bottleneck theory. Copyright (c) 2008 S. Karger AG, Basel"],["dc.identifier.doi","10.1159/000117717"],["dc.identifier.isi","000255175600005"],["dc.identifier.pmid","18418033"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6245"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/55615"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Karger"],["dc.relation.issn","1661-5425"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Subcellular redistribution of the mitochondrial PG2 epitope during development from cleavage to primordial germ cell formation in the rabbit embryo"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","935"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Foot & Ankle International"],["dc.bibliographiccitation.lastpage","941"],["dc.bibliographiccitation.volume","39"],["dc.contributor.author","Hubert, Jan"],["dc.contributor.author","Hawellek, Thelonius"],["dc.contributor.author","Beil, Frank Timo"],["dc.contributor.author","Saul, Dominik"],["dc.contributor.author","Kling, Jens Henning"],["dc.contributor.author","Viebahn, Christoph"],["dc.contributor.author","Jungesblut, Oliver Dirk"],["dc.contributor.author","Stücker, Ralf"],["dc.contributor.author","Rupprecht, Martin"],["dc.date.accessioned","2020-06-09T07:11:04Z"],["dc.date.available","2020-06-09T07:11:04Z"],["dc.date.issued","2018"],["dc.description.abstract","Background: The purpose of the study was to present a novel operative technique in the management of medial talocalcaneal coalition (TC) and to report our clinical and radiologic results after interposition of a pediculated flap (PF) of the tibialis posterior tendon sheath. Methods: Twelve feet of 10 patients with a medial TC were treated with the interposition of PF of the tibialis posterior tendon sheath following resection. Pre- and postoperative clinical examinations were performed to evaluate the range of motion and the function of the tibialis posterior muscle of the affected foot. Pain was registered by visual analog scale (VAS) and the function of the foot by the American Orthopaedic Foot & Ankle Society (AOFAS) hindfoot score. The mean follow-up duration was 57.2 months (SD ±37.2 range 12-128) after surgery. Magnetic resonance imaging (MRI) was carried out to assess the outcome. Results: All patients reported a significant reduction of pain (P = .002) at the final follow-up. The activity level had improved since the operation, and the subtalar joint motion was increased, but no weakness of the tibialis posterior muscle could be observed. The AOFAS hindfoot score was significantly improved (P = .002). MRI did not reveal any migration of the tibialis posterior tendon sheath, and the interposed PF was confirmed at the resection zone. Furthermore, no TC relapse or ruptures of the functional anatomical structures could be observed. Conclusion: The resection combined with the interposition of a PF of the tendon sheath seems to avoid relapse of TC and improves symptoms and the function of the foot."],["dc.identifier.doi","10.1177/1071100718768257"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/66213"],["dc.language.iso","en"],["dc.relation.issn","1071-1007"],["dc.title","Resection of Medial Talocalcaneal Coalition With Interposition of a Pediculated Flap of Tibialis Posterior Tendon Sheath"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","941"],["dc.bibliographiccitation.issue","5929"],["dc.bibliographiccitation.journal","Science"],["dc.bibliographiccitation.lastpage","944"],["dc.bibliographiccitation.volume","324"],["dc.contributor.author","Gros, Jerome"],["dc.contributor.author","Feistel, Kerstin"],["dc.contributor.author","Viebahn, Christoph"],["dc.contributor.author","Blum, Martin"],["dc.contributor.author","Tabin, Clifford J."],["dc.date.accessioned","2018-11-07T08:29:55Z"],["dc.date.available","2018-11-07T08:29:55Z"],["dc.date.issued","2009"],["dc.description.abstract","In vertebrates, the readily apparent left/right (L/R) anatomical asymmetries of the internal organs can be traced to molecular events initiated at or near the time of gastrulation. However, the earliest steps of this process do not seem to be universally conserved. In particular, how this axis is first defined in chicks has remained problematic. Here we show that asymmetric cell rearrangements take place within chick embryos, creating a leftward movement of cells around the node. It is the relative displacement of cells expressing sonic hedgehog (Shh) and fibroblast growth factor 8 (Fgf8) that is responsible for establishing their asymmetric expression patterns. The creation of asymmetric expression domains as a passive effect of cell movements represents an alternative strategy for breaking L/R symmetry in gene activity."],["dc.identifier.doi","10.1126/science.1172478"],["dc.identifier.isi","000266048800040"],["dc.identifier.pmid","19359542"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6244"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/16771"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Assoc Advancement Science"],["dc.relation.issn","0036-8075"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Cell Movements at Hensen's Node Establish Left/Right Asymmetric Gene Expression in the Chick"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","301"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Differentiation"],["dc.bibliographiccitation.lastpage","311"],["dc.bibliographiccitation.volume","78"],["dc.contributor.author","Hassoun, Romia"],["dc.contributor.author","Schwartz, Peter J."],["dc.contributor.author","Feistel, Kerstin"],["dc.contributor.author","Blum, Martin"],["dc.contributor.author","Viebahn, Christoph"],["dc.date.accessioned","2018-11-07T11:21:25Z"],["dc.date.available","2018-11-07T11:21:25Z"],["dc.date.issued","2009"],["dc.description.abstract","Differentiation of the principal body axes in the early vertebrate embryo is based on a specific blueprint of gene expression and a series of transient axial structures such as Hensen's node and the notochord of the late gastrulation phase. Prior to gastrulation, the anterior visceral endoderm (AVE) of the mouse egg-cylinder or the anterior marginal crescent (AMC) of the rabbit embryonic disc marks the anterior pole of the embryo. For phylogenetic and functional reasons both these entities are addressed here as the mammalian anterior pregastrulation differentiation (APD). However, mouse and rabbit show distinct structural differences in APD and the molecular blue print, making the search of general rules for axial differentiation in mammals difficult. Therefore, the pig was analysed here as a further species with a mammotypical flat embryonic disc. Using light and electron microscopy and in situ hybridisation for three key genes involved in early development (sox17, nodal and brachyury), two axial structures of early gastrulation in the pig were identified: (1) the anterior hypoblast (AHB) characterised by increased cellular height and density and by sox17 expression, and (2) the early primitive streak characterised by a high pseudostratified epithelium with an almost continuous but unusually thick basement membrane, by localised epithelial-mesenchymal transition, and by brachyury expression in the epiblast. The stepwise appearance of these two axial structures was used to define three stages typical for mammals at the start of gastrulation. Intriguingly, the round shape and gradual posterior displacement of the APD in the pig appear to be species-specific (differing from all other mammals studied in detail to date) but correlate with ensuing specific primitive streak and extra embryonic mesoderm development. APD and, hence, the earliest axial structure presently known in the mammalian embryo may thus be functionally involved in shaping extra embryonic membranes and, possibly, the specific adult body form. (C) 2009 International Society of Differentiation. Published by Elsevier Ltd. All rights reserved."],["dc.identifier.doi","10.1016/j.diff.2009.07.006"],["dc.identifier.isi","000274532800006"],["dc.identifier.pmid","19683851"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/55765"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Sci Ltd"],["dc.relation.issn","0301-4681"],["dc.title","Axial differentiation and early gastrulation stages of the pig embryo"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","4"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","EvoDevo"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Kremnyov, Stanislav"],["dc.contributor.author","Viebahn, Christoph"],["dc.contributor.author","Tsikolia, Nikoloz"],["dc.contributor.author","Henningfeld, Kristine A."],["dc.date.accessioned","2018-04-18T14:43:20Z"],["dc.date.accessioned","2021-10-27T13:21:04Z"],["dc.date.available","2018-04-18T14:43:20Z"],["dc.date.available","2021-10-27T13:21:04Z"],["dc.date.issued","2018-01-31"],["dc.date.updated","2018-04-18T14:43:20Z"],["dc.description.abstract","Abstract Background The notochord has organizer properties and is required for floor plate induction and dorsoventral patterning of the neural tube. This activity has been attributed to sonic hedgehog (shh) signaling, which originates in the notochord, forms a gradient, and autoinduces shh expression in the floor plate. However, reported data are inconsistent and the spatiotemporal development of the relevant shh expression domains has not been studied in detail. We therefore studied the expression dynamics of shh in rabbit, chicken and Xenopus laevis embryos (as well as indian hedgehog and desert hedgehog as possible alternative functional candidates in the chicken). Results Our analysis reveals a markedly divergent pattern within these vertebrates: whereas in the rabbit shh is first expressed in the notochord and its floor plate domain is then induced during subsequent somitogenesis stages, in the chick embryo shh is expressed in the prospective neuroectoderm prior to the notochord formation and, interestingly, prior to mesoderm immigration. Neither indian hedgehog nor desert hedgehog are expressed in these midline structures although mRNA of both genes was detected in other structures of the early chick embryo. In X. laevis, shh is expressed at the beginning of gastrulation in a distinct area dorsal to the dorsal blastopore lip and adjacent to the prospective neuroectoderm, whereas the floor plate expresses shh at the end of gastrulation. Conclusions While shh expression patterns in rabbit and X. laevis embryos are roughly compatible with the classical view of “ventral to dorsal induction” of the floor plate, the early shh expression in the chick floor plate challenges this model. Intriguingly, this alternative sequence of domain induction is related to the asymmetrical morphogenesis of the primitive node and other axial organs in the chick. Our results indicate that the floor plate in X. laevis and chick embryos may be initially induced by planar interaction within the ectoderm or epiblast. Furthermore, we propose that the mode of the floor plate induction adapts to the variant topography of interacting tissues during gastrulation and notochord formation and thereby reveals evolutionary plasticity of early embryonic induction."],["dc.identifier.doi","10.1186/s13227-017-0090-x"],["dc.identifier.pmid","29423139"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15170"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/91992"],["dc.language.iso","en"],["dc.language.rfc3066","en"],["dc.notes.intern","Migrated from goescholar"],["dc.relation.issn","2041-9139"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","CC BY 4.0"],["dc.rights.holder","The Author(s)"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","610"],["dc.title","Divergent axial morphogenesis and early shh expression in vertebrate prospective floor plate"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","304"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Annals of Anatomy - Anatomischer Anzeiger"],["dc.bibliographiccitation.lastpage","313"],["dc.bibliographiccitation.volume","194"],["dc.contributor.author","Ude-Koeller, Susanne"],["dc.contributor.author","Knauer, Wilfried"],["dc.contributor.author","Viebahn, Christoph"],["dc.date.accessioned","2018-11-07T09:15:12Z"],["dc.date.available","2018-11-07T09:15:12Z"],["dc.date.issued","2012"],["dc.description.abstract","This report briefly summarises anatomical practice at Gottingen University from its founding in 1737 until the Nazi period and gives a detailed account of how Nazi death penalty legislation and execution practice at Wolfenbuttel prison influenced the decision-making of the anatomists in charge at that time. Problems in the procurement of corpses, encountered almost continuously throughout Europe since the broad introduction of dissection into medical training in the early 18th century, were absent in Gottingen during periods of overt progress in anatomical sciences, e.g. under Albrecht von Haller (in office 1736-1753) and Jacob Henle (1853-1885), and at times when existing regulations were rigorously enforced by the authorities (1814-1851). Ample availability of corpses in the wake of more than 600 executions in Wolfenbuttel between 1935 and 1945 was curtailed only by transportation fuel shortages and resulted in the dissection of more than 200 Nazi victim corpses in the Gottingen anatomy course. Apparently, neither individual offers of voluntary body donation (dating from 1932 to 1937 and published here as the earliest documents of this kind), nor the strong tradition of high-level anatomical research, nor even the awareness of the University's Age of Enlightenment origin, prevented the unethical use of corpses of Nazi victims for medical teaching. The Gottingen example may add \"historical and moral detachment\" under unusual political and wartime pressures to the \"clinical and emotional detachment\" thought to prevail amongst anatomy personnel (Hildebrandt, in this issue); together with the other reports it calls for all anatomists to bear in mind their ever present ethical obligations in respect to activities involving the use of corpses, both in medical schools and in the public domain. (c) 2012 Elsevier GmbH. All rights reserved."],["dc.identifier.doi","10.1016/j.aanat.2012.03.002"],["dc.identifier.isi","000306039500012"],["dc.identifier.pmid","22561074"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27622"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Gmbh, Urban & Fischer Verlag"],["dc.relation.issn","0940-9602"],["dc.title","Anatomical practice at Gottingen University since the Age of Enlightenment and the fate of victims from Wolfenbuttel prison under Nazi rule"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1905"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Developmental Dynamics"],["dc.bibliographiccitation.lastpage","1916"],["dc.bibliographiccitation.volume","240"],["dc.contributor.author","Halacheva, Viktoriya"],["dc.contributor.author","Fuchs, Mathias"],["dc.contributor.author","Doenitz, Juergen"],["dc.contributor.author","Reupke, Tobias"],["dc.contributor.author","Pueschel, Bernd"],["dc.contributor.author","Viebahn, Christoph"],["dc.date.accessioned","2018-11-07T08:54:01Z"],["dc.date.available","2018-11-07T08:54:01Z"],["dc.date.issued","2011"],["dc.description.abstract","Formation of the mammalian primitive streak appears to rely on cell proliferation to a minor extent only, but compensating cell movements have not yet been directly observed. This study analyses individual cell migration and proliferation simultaneously, using multiphoton and differential interference contrast time-lapse microscopy of late pregastrulation rabbit blastocysts. Epiblast cells in the posterior gastrula extension area accumulated medially and displayed complex planar movements including U-turns and a novel type of processional cell movement. In the same area metaphase plates tended to be aligned parallel to the anterior-posterior axis, and statistical analysis showed that rotations of metaphase plates causing preferred orientation were near-complete 8 min before anaphase onset; in some cases, rotations were strikingly rapid, achieving up to 45 degrees per min. The mammalian primitive streak appears to be formed initially with its typically minimal anteroposterior elongation by a combination of oriented cell divisions with dedicated planar cell movements. Developmental Dynamics 240:1905-1916, 2011. (C) 2011 Wiley-Liss, Inc."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft [Vi 151/8-1]"],["dc.identifier.doi","10.1002/dvdy.22687"],["dc.identifier.isi","000292772400004"],["dc.identifier.pmid","21761476"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22567"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","1058-8388"],["dc.title","Planar Cell Movements and Oriented Cell Division During Early Primitive Streak Formation in the Mammalian Embryo"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","92"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Development (Cambridge)"],["dc.bibliographiccitation.lastpage","98"],["dc.bibliographiccitation.volume","142"],["dc.contributor.author","Stankova, Viktoria"],["dc.contributor.author","Tsikolia, Nikoloz"],["dc.contributor.author","Viebahn, Christoph"],["dc.date.accessioned","2021-06-01T10:48:14Z"],["dc.date.available","2021-06-01T10:48:14Z"],["dc.date.issued","2015"],["dc.description.abstract","During animal gastrulation, the specification of the embryonic axes is accompanied by epithelio-mesenchymal transition (EMT), the first major change in cell shape after fertilization. EMT takes place in disparate topographical arrangements, such as the circular blastopore of amphibians, the straight primitive streak of birds and mammals or in intermediate gastrulation forms of other amniotes such as reptiles. Planar cell movements are prime candidates to arrange specific modes of gastrulation but there is no consensus view on their role in different vertebrate classes. Here, we test the impact of interfering with Rho kinase-mediated cell movements on gastrulation topography in blastocysts of the rabbit, which has a flat embryonic disc typical for most mammals. Time-lapse video microscopy, electron microscopy, gene expression and morphometric analyses of the effect of inhibiting ROCK activity showed – besides normal specification of the organizer region – a dose-dependent disruption of primitive streak formation; this disruption resulted in circular, arc-shaped or intermediate forms, reminiscent of those found in amphibians, fishes and reptiles. Our results reveal a crucial role of ROCK-controlled directional cell movements during rabbit primitive streak formation and highlight the possibility that temporal and spatial modulation of cell movements were instrumental for the evolution of gastrulation forms."],["dc.identifier.doi","10.1242/dev.111583"],["dc.identifier.isi","000348240500014"],["dc.identifier.pmid","25516971"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85863"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.eissn","1477-9129"],["dc.relation.issn","0950-1991"],["dc.title","Rho kinase activity controls directional cell movements during primitive streak formation in the rabbit embryo"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","275"],["dc.bibliographiccitation.issue","3-4"],["dc.bibliographiccitation.journal","Histochemistry and Cell Biology"],["dc.bibliographiccitation.lastpage","284"],["dc.bibliographiccitation.volume","124"],["dc.contributor.author","Puschel, B."],["dc.contributor.author","Demus, U."],["dc.contributor.author","Viebahn, Christoph"],["dc.date.accessioned","2018-11-07T10:56:12Z"],["dc.date.available","2018-11-07T10:56:12Z"],["dc.date.issued","2005"],["dc.description.abstract","Primordial germ cells represent the founder population for establishing the germ line providing the continuity of life between generations. PG2, a germ cell-specific antigen, is one of the few continuously detectable epitopes in mammalian primordial germ cells and it is dynamically expressed during early post-fertilization development and during postnatal germ cell maturation. Immunoelectron microscopy shows a localization of PG2 in the peri-mitochondrial cytoplasm but its further subcellular or biochemical nature remains elusive. For further characterization of the PG2 epitope we used regular and semi-thin cryosection of ovulated and isolated follicular rabbit oocytes and localized all mitochondria with the help of the constitutive mitochondrial antigen MTC02 in double immunofluorescence stainings. Semi-thin cryosections of ovulated oocytes revealed a general close co-localization of both antibody reactions at the level of single mitochondria. In centrifuged follicular oocytes both antigens co-sedimented almost completely indicating a topographical association of the epitopes on the basis of a strong interaction of PG2 with mitochondria. To begin to characterize the germ cell epitope biochemically we treated oocyte cryosections either with acetone to reduce lipids or with N-glycosidase F to remove N-linked glycosylations before the immuoreaction. Neither treatment affected the antibody characteristics, which suggests that the PG2 epitope is most probably a protein. Because of the close interaction of PG2 with the mitochondria we speculate that PG2 is involved in the change of the mitochondrial morphology typically observed during differentiation of germ cells."],["dc.identifier.doi","10.1007/s00418-005-0041-9"],["dc.identifier.isi","000233012600009"],["dc.identifier.pmid","16088380"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/49956"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1432-119X"],["dc.relation.issn","0948-6143"],["dc.title","Subcellular characterization of the primordial germ cell antigen PG2 in adult oocytes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]