Wolf, Gerhard A.

[["dc.bibliographiccitation.firstpage","113"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","European Journal of Plant Pathology"],["dc.bibliographiccitation.lastpage","124"],["dc.bibliographiccitation.volume","111"],["dc.contributor.author","Eibel, P."],["dc.contributor.author","Wolf, G. A."],["dc.contributor.author","Koch, E."],["dc.date.accessioned","2018-11-07T08:27:32Z"],["dc.date.available","2018-11-07T08:27:32Z"],["dc.date.issued","2005"],["dc.description.abstract","Polyclonal antibodies were raised against mycelium from the logarithmic growth phase of a shake culture of Ustilago nuda, and a double antibody sandwich enzyme-linked immunosorbent assay (ELISA) with biotinylated detection antibodies was developed. The detection limit of the assay was 15 ng total protein m(-1) for the homologous antigen and 50 ng ml(-1) for a spore extract, respectively. Other species of Ustilago reacted with the antibodies. Cross-reactivity was highest with U. tritici. No signal was obtained with the tested isolates of Tilletia, Rhizoctonia, Pythium and Fusarium. With naturally infected barley seeds, the results of the ELISAs were always in good agreement with those obtained with the routinely used seed embryo test. However, when seeds grown from artificially inoculated florets were used, the ELISA indicated significantly higher infestation levels than the embryo test. Results of assays with halved seeds from the same lot showed that high amounts of mycelium were present in the non-embryo half. This and especially the relatively long duration of the assay suggested that the ELISA (as conducted here) may not be suitable as a routine method for analysing seed infection with U. nuda. With samples from barley seedlings grown from infected seeds the results of the immunoassay again corresponded very well with the infection level determined by staining of the seed embryo, irrespective of the mode of floret inoculation (natural or artificial). Potential fields of application of the ELISA include the early prediction of the efficacy of protection agents, e.g. in screenings for seed treatments, the elucidation of the biology of the fungus and characterisation of resistance mechanisms."],["dc.identifier.doi","10.1007/s10658-004-1421-z"],["dc.identifier.isi","000226940100003"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/16225"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0929-1873"],["dc.title","Development and evaluation of an enzyme-linked immunosorbent assay (ELISA) for the detection of loose smut of barley (Ustilago nuda)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","252"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Journal of Plant Diseases and Protection"],["dc.bibliographiccitation.lastpage","258"],["dc.bibliographiccitation.volume","115"],["dc.contributor.author","Wachter, R. Rolf"],["dc.contributor.author","Wolf, G. A."],["dc.contributor.author","Koch, E."],["dc.date.accessioned","2018-11-07T11:08:42Z"],["dc.date.available","2018-11-07T11:08:42Z"],["dc.date.issued","2008"],["dc.description.abstract","Using 30 winter wheat varieties, leaf symptoms indicating infection with common bunt (Tilletia tritici) as well as an enzyme-linked immunosorbent assay (ELISA) based on polyclonal antibodies were evaluated for early assessment of bunt susceptibility/resistance. The percentage of plants with leaf symptoms recorded at growth stage 14 was in most varieties higher when the plants were germinated at 15 degrees C than at 4 degrees C, and the percentage of symptomatic plants was on average higher in the susceptible than in the resistant varieties. However, clue to large variability a general relationship between leaf symptoms and varietal resistance could not be established. It is concluded that leaf symptoms are not generally suited to predict the degree of susceptibility to bunt, irrespective of the temperature at which the plants are geminated. Much better results were obtained with quantification of the pathogen in wheat seedlings by ELISA. In all susceptibility groups and varieties the fungal content of individual plants was highly variable. However, on average the plants expressing early symptoms contained twice as much antigen as the plants without symptoms, and the very highly susceptible varieties contained on average approx. five times as much antigen as the very lowly susceptible ones. While due to high variability it may be more difficult in routine use to accurately assign with the ELISA the varieties of intermediate susceptibility, it is expected that it will be possible to clearly differentiate the lowly and very lowly susceptible varieties from the more susceptible ones, provided a sufficiently large number of plants is assayed."],["dc.identifier.isi","000262697800003"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52845"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Eugen Ulmer Gmbh Co"],["dc.relation.issn","1861-3829"],["dc.title","Evaluation of visual assessment of leaf symptoms and of an enzyme-linked immunosorbent assay as tools for the characterisation of resistance of winter wheat against common bunt (Tilletia tritici [DC] Tul. & C. Tul.)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","694"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Laryngo-Rhino-Otologie"],["dc.bibliographiccitation.lastpage","706"],["dc.bibliographiccitation.volume","99"],["dc.contributor.author","Stöver, T."],["dc.contributor.author","Dazert, S."],["dc.contributor.author","Hoffmann, T. K."],["dc.contributor.author","Plontke, S. K."],["dc.contributor.author","Ambrosch, P."],["dc.contributor.author","Arens, C."],["dc.contributor.author","Betz, C."],["dc.contributor.author","Beutner, D."],["dc.contributor.author","Bohr, C."],["dc.contributor.author","Bruchhage, K.-L."],["dc.contributor.author","Canis, M."],["dc.contributor.author","Dietz, A."],["dc.contributor.author","Guntinas-Lichius, O."],["dc.contributor.author","Hagen, R."],["dc.contributor.author","Hosemann, W."],["dc.contributor.author","Iro, H."],["dc.contributor.author","Klussmann, J.-P."],["dc.contributor.author","Knopf, A."],["dc.contributor.author","Kramer, S."],["dc.contributor.author","Lang, S."],["dc.contributor.author","Leinung, M."],["dc.contributor.author","Lenarz, T."],["dc.contributor.author","Löwenheim, H."],["dc.contributor.author","Matthias, C."],["dc.contributor.author","Mlynski, R."],["dc.contributor.author","Olze, H."],["dc.contributor.author","Park, J."],["dc.contributor.author","Plinkert, P."],["dc.contributor.author","Radeloff, A."],["dc.contributor.author","Rotter, N."],["dc.contributor.author","Rudack, C."],["dc.contributor.author","Bozzato, A."],["dc.contributor.author","Schipper, J."],["dc.contributor.author","Schrader, M."],["dc.contributor.author","Strieth, S."],["dc.contributor.author","Stuck, B. A."],["dc.contributor.author","Volkenstein, S."],["dc.contributor.author","Westhofen, M."],["dc.contributor.author","Wolf, G."],["dc.contributor.author","Wollenberg, B."],["dc.contributor.author","Zahnert, T."],["dc.contributor.author","Zenk, J."],["dc.date.accessioned","2021-04-14T08:23:07Z"],["dc.date.available","2021-04-14T08:23:07Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1055/a-1232-4911"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80799"],["dc.language.iso","de"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1438-8685"],["dc.relation.issn","0935-8943"],["dc.title","Auswirkungen der SARS-CoV-2-Pandemie auf die universitäre Hals-Nasen-Ohrenheilkunde im Bereich der Krankenversorgung"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1365"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","European Food Research and Technology"],["dc.bibliographiccitation.lastpage","1371"],["dc.bibliographiccitation.volume","226"],["dc.contributor.author","Wang, Jinhua"],["dc.contributor.author","Pawelzik, Elke"],["dc.contributor.author","Weinert, Joachim"],["dc.contributor.author","Zhao, Qinghua"],["dc.contributor.author","Wolf, Gerhard A."],["dc.date.accessioned","2018-09-10T09:04:32Z"],["dc.date.available","2018-09-10T09:04:32Z"],["dc.date.issued","2007"],["dc.description.abstract","The Hagberg falling number (HFN) is one of the most important quality characteristics of wheat. Six winter wheat cultivars from three locations in Germany were analysed to assess the factors influencing the HFN. The cultivars and locations affected significantly the HFN of all the samples. The infection with Fusarium culmorum, determined in another cultivar, also led to an increase in HFN, which suggested that the HFN method is not adequate enough for the true determination of the alpha-amylase activity of fungus-infected, wheat flour. Furthermore, there was no clear correlation between HFN and thousand-kernel weight, starch content or pentosan content. The crude protein content was positively related to the HFN, while the total dietary fibre content depended on the cultivar and location."],["dc.identifier.doi","10.1007/s00217-007-0666-0"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/15689"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.issn","1438-2385"],["dc.title","Factors influencing falling number in winter wheat"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","190"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Annals of the Rheumatic Diseases"],["dc.bibliographiccitation.lastpage","195"],["dc.bibliographiccitation.volume","59"],["dc.contributor.author","Berner, B."],["dc.contributor.author","Wolf, G."],["dc.contributor.author","Hummel, Klaus M."],["dc.contributor.author","Mueller, Gerhard A."],["dc.contributor.author","Reuss-Borst, M. A."],["dc.date.accessioned","2018-11-07T08:55:21Z"],["dc.date.available","2018-11-07T08:55:21Z"],["dc.date.issued","2000"],["dc.description.abstract","Objectives-The interaction between the activation induced surface glycoprotein CD40L (ligand) (CD154) on CD4+ T cells and its receptor CD40, which is expressed on various cell types, plays a crucial part in numerous cell mediated and humoral immune reactions that may be of pathogenetic importance in rheumatoid arthritis (RA). To further evaluate the pathogenetic role of CD40L in RA, expression of CD40L and various other T cell activation antigens as well as costimulatory molecules was investigated on CD4+ T cells in RA by flow cytometry. Methods-Two colour flow cytometry was used to determine the percentage of CD4+ T cells expressing CD40L, CD69, CD25, HLA-DR, CD39, CD27 and CD28 in peripheral blood (PB) of 62 RA patients in comparison to 20 healthy controls (HC). Disease activity was assessed by clinical, laboratory and radiological examination. Status of clinical remission of RA was evaluated according to the ACR preliminary criteria for complete clinical remission of RA. Results-CD40L was expressed on > 10% of CD4+ T cells in 29% of RA patients thus defining a CD40L(high+) patient group. Disease activity as estimated by C reactive protein, rheumatoid factor and status of clinical remission of disease (p = 0.049) was higher in this subgroup than in the RA CD40L(low+) group. Expression of CD69, CD25, and HLA-DR was significantly increased in both RA patient groups in comparison with HC. However, the percentage of CD39+ CD4+ T cells was increased only in the RA CD40L(high+) subgroup (versus HC p = 0.019, versus RA CD40(low+) p = 0.044). Furthermore, expression of CD40L and CD39 on CD4+ T cells correlated positively as estimated by Spearman rank correlation (p < 0.001). The percentage of CD4+ T cells lacking the costimulatory molecules CD27 (p 0.002) and CD28 (p = 0.026) was increased in RA CD40L(low+) patients in comparison with HC. Conclusions-These data suggest that increased expression of CD40L on CD4+ T cells in RA indicates prolonged and increased activation of CD4+ T lymphocytes and is associated with active disease and possibly an unfavourable prognosis. Whether this phenotypically defined RA CD40L(high+) subgroup will preferentially respond to an anti-CD40L antibody treatment remains to be elucidated."],["dc.identifier.doi","10.1136/ard.59.3.190"],["dc.identifier.isi","000085777200007"],["dc.identifier.pmid","10700427"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22880"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","British Med Journal Publ Group"],["dc.relation.issn","0003-4967"],["dc.title","Increased expression of CD40 ligand (CD154) on CD4+T cells as a marker of disease activity in rheumatoid arthritis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","5818"],["dc.bibliographiccitation.issue","14"],["dc.bibliographiccitation.journal","Journal of Agricultural and Food Chemistry"],["dc.bibliographiccitation.lastpage","5823"],["dc.bibliographiccitation.volume","53"],["dc.contributor.author","Wang, J."],["dc.contributor.author","Pawelzik, Elke"],["dc.contributor.author","Weinert, Joachim"],["dc.contributor.author","Wolf, G. A."],["dc.date.accessioned","2018-11-07T08:42:29Z"],["dc.date.available","2018-11-07T08:42:29Z"],["dc.date.issued","2005"],["dc.description.abstract","To assess the effects of Fusarium infection on the polysaccharides of winter wheat grain (Triticum aestivum L.), grain samples obtained from plants artificially inoculated with Fusarium culmorum were analyzed. Microscopy revealed obvious damage to the starch granules in the seriously infected samples. The Fusarium infection had no analytically detectable influence on the starch and total insoluble dietary fiber content of the wheat grain. There were significantly positive relationships between alpha-amylase activity, cellulase activity, total soluble dietary fiber content, pentosan content, and degree of infection quantified by an enzyme-linked immunosorbant assay, which would indicate the importance of fungal enzymes. A distinct higher Hagberg failing number (FN) was determined in the seriously infected samples, while the viscosity and sucrose content of the flour decreased. However, the addition of a liquid medium contaminated with F. culmorum led to a significant decrease in the FN. Depending on the type of buffer used, the alpha-amylase of F culmorum demonstrated its maximum activity between pH 5.5 and pH 7.0 at 30-50 degrees C. Remarkably, this fungal alpha-amylase showed a thermostable, characteristic and was active over a wide range of temperatures, from 10 to 100 degrees C. This type of thermostability suggests that the alpha-amylase of F. culmorum may damage starch granules throughout the processing of wheat flour, thereby inducing weak dough properties and unsatisfactory bread quality."],["dc.identifier.doi","10.1021/jf050525g"],["dc.identifier.isi","000230357200051"],["dc.identifier.pmid","15998153"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/19713"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Chemical Soc"],["dc.relation.issn","0021-8561"],["dc.title","Impact of Fusarium culmorum on the polysaccharides of wheat flour"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Zeitschrift für Rheumatologie"],["dc.bibliographiccitation.volume","72"],["dc.contributor.author","Trautmann, Sandra"],["dc.contributor.author","Beham, Alexander W."],["dc.contributor.author","Mai, Burkhard"],["dc.contributor.author","Breysach, Caroline"],["dc.contributor.author","Wolf, G."],["dc.contributor.author","Mueller, G."],["dc.contributor.author","Blaschke, S."],["dc.date.accessioned","2018-11-07T09:20:02Z"],["dc.date.available","2018-11-07T09:20:02Z"],["dc.date.issued","2013"],["dc.format.extent","47"],["dc.identifier.isi","000331709100073"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28783"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","Heidelberg"],["dc.relation.issn","1435-1250"],["dc.relation.issn","0340-1855"],["dc.title","Regulation of osteoclast bone resorptive activity in rheumatoid arthritis"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","461"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","ZEITSCHRIFT FUR PFLANZENKRANKHEITEN UND PFLANZENSCHUTZ-JOURNAL OF PLANT DISEASES AND PROTECTION"],["dc.bibliographiccitation.lastpage","464"],["dc.bibliographiccitation.volume","111"],["dc.contributor.author","Tian, S. M."],["dc.contributor.author","Weinert, Joachim"],["dc.contributor.author","Wolf, G. A."],["dc.date.accessioned","2018-11-07T10:46:16Z"],["dc.date.available","2018-11-07T10:46:16Z"],["dc.date.issued","2004"],["dc.description.abstract","Field trials containing triticale and wheat cultivars were conducted in 2000-2002 at Goettingen, Germany. In 200 1, a high level of infection by Puccinia striiformis (yellow rust) occurred. Symptoms of the disease on spikes were described. The cultivars of triticale showed significant differences in percentage of infected leaf area (0.2-56.2 %) and infected spikelets per ear (1.1-42.6 %). Grain yield loss caused by yellow rust ranged from 0.4 % to 21.5 %, compared to fungicide-treated plots. The susceptible wheat cultivars were also severely infected by P. striiformis on leaves, but rarely on spikes. To our knowledge, this is the first report on the severity of yellow rust infection on triticale and on the yield toss caused by the disease in Germany."],["dc.identifier.isi","000225649000005"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/47707"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Eugen Ulmer Gmbh Co"],["dc.relation.issn","0340-8159"],["dc.title","Infection of triticale cultivars by Puccinia striiformis: first report on disease severity and yield loss"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","815"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Proceeding of the Bulgarian Academy of Sciences"],["dc.bibliographiccitation.lastpage","820"],["dc.bibliographiccitation.volume","67"],["dc.contributor.author","Koleva, Lilyana"],["dc.contributor.author","Ulber, Bernd"],["dc.contributor.author","Wolf, Gerhard A."],["dc.date.accessioned","2018-11-07T09:45:57Z"],["dc.date.available","2018-11-07T09:45:57Z"],["dc.date.issued","2014"],["dc.description.abstract","The investigations of the effect of Collembola species O. fimatus or F. candida on the infection of sugar beet with P. ultimum were carried out under laboratory conditions. The ability of collembolans to reduce the infestation with soil-borne plant pathogenic fungus P. ultimum was studied directly in the roots of sugar beet seedlings. The influence of Collembola on Pythium infection was assessed by comparing the xylanase and cellulase activity in the plants, which had been grown in the P. ultimum - inoculated soil with or without collembolans. Results revealed that collembola species O. fimatus and F. candida significantly reduced infection of the plant by P. ultimum."],["dc.identifier.isi","000339698000011"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/34755"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.notes.submitter","Najko"],["dc.relation.issn","1310-1331"],["dc.title","Studies on the Effect Of Collembola Species Onychiurus Fimatus Gisin (Onychiuridae) and Folsomia Candida Willem (Isotomidae) on the Infection of Sugar Beet with Pythium Ultimum Trow"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","44"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Journal of Integrative Plant Biology"],["dc.bibliographiccitation.lastpage","52"],["dc.bibliographiccitation.volume","48"],["dc.contributor.author","Dai, F. M."],["dc.contributor.author","Xu, T."],["dc.contributor.author","Wolf, G. A."],["dc.contributor.author","He, Z. H."],["dc.date.accessioned","2018-11-07T10:36:43Z"],["dc.date.available","2018-11-07T10:36:43Z"],["dc.date.issued","2006"],["dc.description.abstract","The fungal pathogen Sclerotinia sclerotiorum Libert causes rot diseases on many crops worldwide and large economic losses occur frequently because of a lack of resistant varieties. The pathogenesis of S. sclerotiorum and the molecular basis of plant responses to the pathogen are poorly understood. In the present investigation, the process of S. sclerotiorum infection in Arabidopsis thaliana L., a plant that is highly susceptible to this fungus, was analysed. In addition, the defense activation in the host was investigated. A convenient inoculation method using millet grain was developed for S. sclerotiorum in Arabidopsis. The fungus rapidly infected the plants, probably through ball- or cushion-like infection structures. Visible symptoms developed within 24 h and plants were killed 72 h after inoculation. Cellulase, the main enzyme that caused host tissues to rot, was secreted by S. sclerotiorum in a pH-dependent manner. Oxalic acid, another pathogenic factor secreted by the fungus, induced necrotic lesions on the leaves. Infection with S. sclerotiorum, strongly induced the production of the pathogenesis-related (PR) proteins beta-1,3-glucanase and chitinase in Arabidopsis. Furthermore, the PR gene PDF.1 was induced, but not PR1, indicating that the pathogen activated basal defense of jasmonic acid/ethylene dependence, which is consistent with its necrotrophic characteristics. This pathosystem for Arabidopsis-S. scierotiorum could provide an approach for the analysis of the interactions between S. scierotiorum and other crops, thereby facilitating genetic manipulation techniques for controlling this pathogen."],["dc.identifier.isi","000234543100006"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/45391"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Hoboken"],["dc.relation.conference","Workshop on Arabidopsis Research in China"],["dc.relation.eventlocation","Shanghai, PEOPLES R CHINA"],["dc.relation.issn","1744-7909"],["dc.relation.issn","1672-9072"],["dc.title","Physiological and molecular features of the pathosystem Arabidopsis thaliana L.-Sclerotinia sclerotiorum Libert"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]