Fricke, Christian

[["dc.bibliographiccitation.firstpage","19953"],["dc.bibliographiccitation.issue","38"],["dc.bibliographiccitation.journal","The Journal of Biological Chemistry"],["dc.bibliographiccitation.lastpage","19961"],["dc.bibliographiccitation.volume","291"],["dc.contributor.author","Gleisner, Martin"],["dc.contributor.author","Kroppen, Benjamin"],["dc.contributor.author","Fricke, Christian"],["dc.contributor.author","Teske, Nelli"],["dc.contributor.author","Kliesch, Torben-Tobias"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Meinecke, Michael"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2020-12-10T18:12:56Z"],["dc.date.available","2020-12-10T18:12:56Z"],["dc.date.issued","2016"],["dc.description.abstract","The epsin N-terminal homology domain (ENTH) is a major player in clathrin-mediated endocytosis. To investigate the influence of initial membrane tension on ENTH binding and activity, we established a bilayer system based on adhered giant unilamellar vesicles (GUVs) to be able to control and adjust the membrane tension sigma covering a broad regime. The shape of each individual adhered GUV as well as its adhesion area was monitored by spinning disc confocal laser microscopy. Control of sigma in a range of 0.08-1.02 mN/m was achieved by altering the Mg2+ concentration in solution, which changes the surface adhesion energy per unit area of the GUVs. Specific binding of ENTH to phosphatidylinositol 4,5-bisphosphate leads to a substantial increase in adhesion area of the sessile GUV. At low tension (<0.1 mN/m) binding of ENTH can induce tubular structures, whereas at higher membrane tension the ENTH interaction deflates the sessile GUV and thereby increases the adhesion area. The increase in adhesion area is mainly attributed to a decrease in the area compressibility modulus K-A. We propose that the insertion of the ENTH helix-0 into the membrane is largely responsible for the observed decrease in K-A, which is supported by the observation that the mutant ENTH L6E shows a reduced increase in adhesion area. These results demonstrate that even in the absence of tubule formation, the area compressibility modulus and, as such, the bending rigidity of the membrane is considerably reduced upon ENTH binding. This renders membrane bending and tubule formation energetically less costly."],["dc.identifier.doi","10.1074/jbc.M116.731612"],["dc.identifier.eissn","1083-351X"],["dc.identifier.gro","3141621"],["dc.identifier.isi","000383243100019"],["dc.identifier.issn","0021-9258"],["dc.identifier.pmid","27466364"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/74538"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1083-351X"],["dc.relation.issn","0021-9258"],["dc.relation.orgunit","Institut für Zellbiochemie"],["dc.title","Epsin N-terminal Homology Domain (ENTH) Activity as a Function of Membrane Tension"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","243"],["dc.bibliographiccitation.lastpage","245"],["dc.bibliographiccitation.seriesnr","9"],["dc.contributor.author","Hofmann, M."],["dc.contributor.author","Fricke, C."],["dc.contributor.author","Isselstein, J."],["dc.contributor.editor","Lüscher, A."],["dc.contributor.editor","Jeangros, B."],["dc.contributor.editor","Huguenin, O."],["dc.contributor.editor","Lobsiger, M."],["dc.contributor.editor","Millar, N."],["dc.contributor.editor","Suter, D."],["dc.date.accessioned","2017-09-07T11:52:11Z"],["dc.date.available","2017-09-07T11:52:11Z"],["dc.date.issued","2004"],["dc.description.abstract","Semi-natural, species-rich hay meadows have declined drastically in recent years. Seed spread deliberately into an Arrhenatherum elatius-stand was used to examine the impact of management measures on sward structure and the resulting establishment success. The tested factors comprised irrigation, cutting and harrowing, which were orthogonally combined in a split-plot design with 4 replications. Seeds of 6 wildflower and 2 grass species were spread over the sward and emergence and survival of seedlings were recorded. Seedling emergence varied between 3 % for Lychnis flos-cuculi and 18 % for Plantago lanceolata. Frequent cutting was the most important factor for a high seedling emergence. On average, twice as many seedlings emerged in cut swards than in uncut swards. Harrowing before spreading seeds enhanced seedling emergence of two species, regular irregation of one species. Survival of emerged seedlings in cut swards was higher than in uncut ones, irrigation and harrowing of the sward were of minor importance. It is concluded that for the diversification of productive grassland by oversowing, in the beginning, frequent cutting of the sward is necessary for successful seedling establishment."],["dc.identifier.gro","3148111"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/5461"],["dc.language.iso","en"],["dc.notes.status","fcwi"],["dc.notes.submitter","chake"],["dc.publisher","EGF"],["dc.relation.conference","20th General Meeting of the European Grassland Federation"],["dc.relation.crisseries","Grassland Science in Europe"],["dc.relation.eventend","2004-06-24"],["dc.relation.eventlocation","Luzern, Switzerland"],["dc.relation.eventstart","2004-06-21"],["dc.relation.isbn","3-7281-2940-2"],["dc.relation.ispartof","Land Use Systems in Grassland Dominated Regions"],["dc.relation.ispartofseries","Grassland Science in Europe; 9"],["dc.title","Establishment of experimentally introduced plant species in a hay meadow"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","111351"],["dc.bibliographiccitation.journal","Journal of Molecular Liquids"],["dc.bibliographiccitation.volume","291"],["dc.contributor.author","Kahrs, Catharina"],["dc.contributor.author","Metze, Michael"],["dc.contributor.author","Fricke, Christian"],["dc.contributor.author","Schwellenbach, Jan"],["dc.date.accessioned","2020-12-10T15:20:23Z"],["dc.date.available","2020-12-10T15:20:23Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1016/j.molliq.2019.111351"],["dc.identifier.issn","0167-7322"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/72651"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Thermodynamic analysis of polymer solutions for the production of polymeric membranes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]