Chizhik, Anna M.

[["dc.bibliographiccitation.artnumber","204201"],["dc.bibliographiccitation.issue","20"],["dc.bibliographiccitation.journal","The Journal of Chemical Physics"],["dc.bibliographiccitation.volume","148"],["dc.contributor.author","Karedla, Narain"],["dc.contributor.author","Chizhik, Anna M."],["dc.contributor.author","Stein, Simon C"],["dc.contributor.author","Ruhlandt, Daja"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Chizhik, Alexey I."],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2020-05-29T09:29:43Z"],["dc.date.available","2020-05-29T09:29:43Z"],["dc.date.issued","2018-05-28"],["dc.description.abstract","Our paper presents the first theoretical and experimental study using single-molecule Metal-Induced Energy Transfer (smMIET) for localizing single fluorescent molecules in three dimensions. Metal-Induced Energy Transfer describes the resonant energy transfer from the excited state of a fluorescent emitter to surface plasmons in a metal nanostructure. This energy transfer is strongly distance-dependent and can be used to localize an emitter along one dimension. We have used Metal-Induced Energy Transfer in the past for localizing fluorescent emitters with nanometer accuracy along the optical axis of a microscope. The combination of smMIET with single-molecule localization based super-resolution microscopy that provides nanometer lateral localization accuracy offers the prospect of achieving isotropic nanometer localization accuracy in all three spatial dimensions. We give a thorough theoretical explanation and analysis of smMIET, describe its experimental requirements, also in its combination with lateral single-molecule localization techniques, and present first proof-of-principle experiments using dye molecules immobilized on top of a silica spacer, and of dye molecules embedded in thin polymer films."],["dc.identifier.doi","10.1063/1.5027074"],["dc.identifier.pmid","29865842"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/66012"],["dc.language.iso","en"],["dc.relation.eissn","1089-7690"],["dc.relation.issn","0021-9606"],["dc.title","Three-dimensional single-molecule localization with nanometer accuracy using Metal-Induced Energy Transfer (MIET) imaging"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","11839"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","ACS Nano"],["dc.bibliographiccitation.lastpage","11846"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Chizhik, Anna M."],["dc.contributor.author","Ruhlandt, Daja"],["dc.contributor.author","Pfaff, Janine"],["dc.contributor.author","Karedla, Narain"],["dc.contributor.author","Chizhik, Alexey I."],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Kehlenbach, Ralph H."],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2018-04-23T11:48:40Z"],["dc.date.available","2018-04-23T11:48:40Z"],["dc.date.issued","2017"],["dc.description.abstract","The nuclear envelope, comprising the inner and the outer nuclear membrane, separates the nucleus from the cytoplasm and plays a key role in cellular functions. Nuclear pore complexes (NPCs), which are embedded in the nuclear envelope, control transport of macromolecules between the two compartments. Here, using dual-color metal-induced energy transfer (MIET), we determine the axial distance between Lap2β and Nup358 as markers for the inner nuclear membrane and the cytoplasmic side of the NPC, respectively. Using MIET imaging, we reconstruct the 3D profile of the nuclear envelope over the whole basal area, with an axial resolution of a few nanometers. This result demonstrates that optical microscopy can achieve nanometer axial resolution in biological samples and without recourse to complex interferometric approaches."],["dc.identifier.doi","10.1021/acsnano.7b04671"],["dc.identifier.gro","3142097"],["dc.identifier.pmid","28921961"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13555"],["dc.identifier.url","https://sfb1190.med.uni-goettingen.de/production/literature/publications/13"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.status","final"],["dc.relation","SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente"],["dc.relation","SFB 1190 | P07: Dynamik von Proteinen der inneren Kernmembran"],["dc.relation.issn","1936-0851"],["dc.relation.workinggroup","RG Kehlenbach (Nuclear Transport)"],["dc.title","Three-Dimensional Reconstruction of Nuclear Envelope Architecture Using Dual-Color Metal-Induced Energy Transfer Imaging"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","265"],["dc.bibliographiccitation.lastpage","281"],["dc.contributor.author","Karedla, Narain"],["dc.contributor.author","Ruhlandt, Daja"],["dc.contributor.author","Chizhik, Anna M."],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Chizhik, Alexey I."],["dc.date.accessioned","2020-05-29T09:29:20Z"],["dc.date.available","2020-05-29T09:29:20Z"],["dc.date.issued","2014"],["dc.identifier.doi","10.1007/4243_2014_77"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/66008"],["dc.relation.isbn","978-3-319-15635-4"],["dc.relation.isbn","978-3-319-15636-1"],["dc.relation.ispartof","Advanced Photon Counting"],["dc.title","Metal-Induced Energy Transfer"],["dc.type","book_chapter"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","237"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nano Letters"],["dc.bibliographiccitation.lastpage","242"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Chizhik, Anna M."],["dc.contributor.author","Stein, Simon"],["dc.contributor.author","Dekaliuk, Mariia O."],["dc.contributor.author","Battle, Christopher"],["dc.contributor.author","Li, Weixing"],["dc.contributor.author","Huss, Anja"],["dc.contributor.author","Platen, Mitja"],["dc.contributor.author","Schaap, Iwan A. T."],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Demchenko, Alexander P."],["dc.contributor.author","Schmidt, Christoph F."],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Chizhik, Alexey"],["dc.date.accessioned","2017-09-07T11:54:46Z"],["dc.date.available","2017-09-07T11:54:46Z"],["dc.date.issued","2016"],["dc.description.abstract","Success in super-resolution imaging relies on a proper choice of fluorescent probes. Here, we suggest novel easily produced and biocompatible nanoparticles-carbon nanodots-for super-resolution optical fluctuation bioimaging (SOFT). The particles revealed an intrinsic dual-color fluorescence, which corresponds to two subpopulations of particles of different electric charges. The neutral nanoparticles localize to cellular nuclei suggesting their potential use as an inexpensive, easily produced nucleus-specific label. The single particle study revealed that the carbon nanodots possess a unique hybrid combination of fluorescence properties exhibiting characteristics of both dye molecules and semiconductor nanocrystals. The results suggest that charge trapping and redistribution on the surface of the particles triggers their transitions between emissive and dark states. These findings open up new possibilities for the utilization of carbon nanodots in the various super-resolution microscopy methods based on stochastic optical switching."],["dc.identifier.doi","10.1021/acs.nanolett.5b03609"],["dc.identifier.gro","3141754"],["dc.identifier.isi","000368322700038"],["dc.identifier.pmid","26605640"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/702"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1530-6992"],["dc.relation.issn","1530-6984"],["dc.title","Super-Resolution Optical Fluctuation Bio-Imaging with Dual-Color Carbon Nanodots"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","173002"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","17"],["dc.bibliographiccitation.journal","Physical Review Letters"],["dc.bibliographiccitation.lastpage","5"],["dc.bibliographiccitation.volume","115"],["dc.contributor.author","Karedla, Narain"],["dc.contributor.author","Stein, Simon C."],["dc.contributor.author","Hähnel, Dirk"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Chizhik, Anna"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2018-04-23T11:49:21Z"],["dc.date.available","2018-04-23T11:49:21Z"],["dc.date.issued","2015"],["dc.description.abstract","The emission properties of most fluorescent emitters, such as dye molecules or solid-state color centers, can be well described by the model of an oscillating electric dipole. However, the orientations of their excitation and emission dipoles are, in most cases, not parallel. Although single molecule excitation and emission dipole orientation measurements have been performed in the past, no experimental method has so far looked at the three-dimensional excitation and emission dipole geometry of individual emitters simultaneously. We present the first experimental study, using defocused imaging in conjunction with radially polarized excitation scanning, to measure both the excitation as well as emission dipole orientations of single molecules, which allows us to sample the distribution of their mutual orientation. We find an unexpectedly broad distribution of the angle between both dipoles which we attribute to the interaction between the observed molecules and the substrate they are immobilized on."],["dc.identifier.doi","10.1103/PhysRevLett.115.173002"],["dc.identifier.gro","3142108"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13677"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.status","final"],["dc.relation.eissn","1079-7114"],["dc.relation.issn","0031-9007"],["dc.relation.issn","0031-9007"],["dc.title","Simultaneous Measurement of the Three-Dimensional Orientation of Excitation and Emission Dipoles"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1695"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Nano Letters"],["dc.bibliographiccitation.lastpage","1700"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Ghosh, Subhabrata"],["dc.contributor.author","Chizhik, Anna M."],["dc.contributor.author","Yang, Gaoling"],["dc.contributor.author","Karedla, Narain"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Oron, Dan"],["dc.contributor.author","Weiss, Shimon"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Chizhik, Alexey I."],["dc.date.accessioned","2020-12-10T18:09:12Z"],["dc.date.available","2020-12-10T18:09:12Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1021/acs.nanolett.8b04695"],["dc.identifier.eissn","1530-6992"],["dc.identifier.issn","1530-6984"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/73565"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Excitation and Emission Transition Dipoles of Type-II Semiconductor Nanorods"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","9429"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Optics Express"],["dc.bibliographiccitation.lastpage","9445"],["dc.bibliographiccitation.volume","24"],["dc.contributor.author","Isbaner, Sebastian"],["dc.contributor.author","Karedla, Narain"],["dc.contributor.author","Ruhlandt, Daja"],["dc.contributor.author","Stein, Simon Christoph"],["dc.contributor.author","Chizhik, Anna"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2021-11-22T14:31:36Z"],["dc.date.available","2021-11-22T14:31:36Z"],["dc.date.issued","2016"],["dc.description.abstract","We present a comprehensive theory of dead-time effects on Time-Correlated Single Photon Counting (TCSPC) as used for fluorescence lifetime measurements, and develop a correction algorithm to remove these artifacts. We apply this algorithm to fluorescence lifetime measurements as well as to Fluorescence Lifetime Imaging Microscopy (FLIM), where rapid data acquisition is necessarily connected with high count rates. There, dead-time effects cannot be neglected, and lead to distortions in the observed lifetime image. The algorithm is quite general and completely independent of the particular nature of the measured signal. It can also be applied to any other single-event counting measurement with detector and/or electronics dead-time."],["dc.identifier.doi","10.1364/OE.24.009429"],["dc.identifier.gro","3142106"],["dc.identifier.pmid","27137558"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14123"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/93392"],["dc.language.iso","en"],["dc.notes.intern","Migrated from goescholar"],["dc.notes.status","final"],["dc.relation.issn","1094-4087"],["dc.rights.access","openAccess"],["dc.subject","fluorescence; lifetime; imaging"],["dc.title","Dead-time correction of fluorescence lifetime measurements and fluorescence lifetime imaging"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","21306"],["dc.bibliographiccitation.issue","41"],["dc.bibliographiccitation.journal","Nanoscale"],["dc.bibliographiccitation.lastpage","21315"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Zelená, Anna"],["dc.contributor.author","Isbaner, Sebastian"],["dc.contributor.author","Ruhlandt, Daja"],["dc.contributor.author","Chizhik, Anna"],["dc.contributor.author","Cassini, Chiara"],["dc.contributor.author","Klymchenko, Andrey S."],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Chizhik, Alexey"],["dc.contributor.author","Köster, Sarah"],["dc.date.accessioned","2021-04-14T08:31:45Z"],["dc.date.available","2021-04-14T08:31:45Z"],["dc.date.issued","2020"],["dc.description.abstract","Human blood platelets are non-nucleated fragments of megakaryocytes and of high importance for early hemostasis. To form a blood clot, platelets adhere to the blood vessel wall, spread and attract other platelets. Despite the importance for biomedicine, the exact mechanism of platelet spreading and adhesion to surfaces remains elusive. Here, we employ metal-induced energy transfer (MIET) imaging with a leaflet-specific fluorescent membrane probe to quantitatively determine, with nanometer resolution and in a time-resolved manner, the height profile of the basal and the apical platelet membrane above a rigid substrate during platelet spreading. We observe areas, where the platelet membrane approaches the substrate particularly closely and these areas are stable on a time scale of minutes. Time-resolved MIET measurements reveal distinct behaviors of the outermost rim and the central part of the platelets, respectively. Our findings quantify platelet adhesion and spreading and improve our understanding of early steps in blood clotting. Furthermore, the results of this study demonstrate the potential of MIET for simultaneous imaging of two close-by membranes and thus three-dimensional reconstruction of the cell shape."],["dc.identifier.doi","10.1039/d0nr05611a"],["dc.identifier.pmid","33073832"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83702"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/150"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","2040-3372"],["dc.relation.issn","2040-3364"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.relation.workinggroup","RG Enderlein"],["dc.rights","CC BY-NC 3.0"],["dc.subject.gro","cellular biophysics"],["dc.title","Time-resolved MIET measurements of blood platelet spreading and adhesion"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","9907"],["dc.bibliographiccitation.issue","13"],["dc.bibliographiccitation.journal","Optics Express"],["dc.bibliographiccitation.lastpage","9917"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Khoptyar, Dmitry"],["dc.contributor.author","Gutbrod, Raphael"],["dc.contributor.author","Chizhik, Anna"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Schleifenbaum, Frank"],["dc.contributor.author","Steiner, Mathias"],["dc.contributor.author","Meixner, Alfred J."],["dc.date.accessioned","2018-04-23T11:47:01Z"],["dc.date.available","2018-04-23T11:47:01Z"],["dc.date.issued","2008"],["dc.description.abstract","We evaluate the field distribution in the focal spot of the fundamental Gaussian beam as well as radially and azimuthally polarized doughnut beams focused inside a planar metallic sub-wavelength microcavity using a high numerical aperture objective lens. We show that focusing in the cavity results in a much tighter focal spot in longitudinal direction compared to free space and in spatial discrimination between longitudinal and in-plane field components. In order to verify the modeling results we experimentally monitor excitation patterns of fluorescence beads inside the λ/2-cavity and find them in full agreement to the modeling predictions. We discuss the implications of the results for cavity assisted single molecular spectroscopy and intra-cavity single molecular imaging."],["dc.identifier.doi","10.1364/oe.16.009907"],["dc.identifier.gro","3142167"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13284"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.status","final"],["dc.relation.eissn","1094-4087"],["dc.relation.issn","1094-4087"],["dc.title","Tight focusing of laser beams in a λ/2-microcavity"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.contributor.author","Thiele, Jan Christoph"],["dc.contributor.author","Jungblut, Marvin"],["dc.contributor.author","Helmerich, Dominic A."],["dc.contributor.author","Tsukanov, Roman"],["dc.contributor.author","Chizhik, Anna M."],["dc.contributor.author","Chizhik, Alexey I."],["dc.contributor.author","Schnermann, Martin"],["dc.contributor.author","Sauer, Markus"],["dc.contributor.author","Nevskyi, Oleksii"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2022-01-12T16:50:43Z"],["dc.date.available","2022-01-12T16:50:43Z"],["dc.date.issued","2021-12-22"],["dc.description.abstract","Over the last two decades, super-resolution microscopy has seen a tremendous development in speed and resolution, but for most of its methods, there exists a remarkable gap between lateral and axial resolution. Similar to conventional optical microscopy, the axial resolution is by a factor three to five worse than the lateral resolution. One recently developed method to close this gap is metal-induced energy transfer (MIET) imaging which achieves an axial resolution down to nanometers. It exploits the distance dependent quenching of fluorescence when a fluorescent molecule is brought close to a metal surface. In the present manuscript, we combine the extreme axial resolution of MIET imaging with the extraordinary lateral resolution of single-molecule localization microscopy, in particular with direct stochastic optical reconstruction microscopy (dSTORM). This combination allows us to achieve isotropic three-dimensional super-resolution imaging of sub-cellular structures. Moreover, we employed spectral demixing for implementing dualcolor MIET-dSTORM that allows us to image and co-localize, in three dimensions, two different cellular structures simultaneously."],["dc.format.extent","16"],["dc.identifier.doi","10.1101/2021.12.20.473473"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/98094"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/378"],["dc.language.iso","en"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.orgunit","III. Physikalisches Institut - Biophysik"],["dc.relation.workinggroup","RG Enderlein"],["dc.title","Isotropic Three-Dimensional Dual-Color Super-Resolution Microscopy with Metal-Induced Energy Transfer"],["dc.type","preprint"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]