Großhans, Jörg

[["dc.bibliographiccitation.artnumber","e2003315"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","PLOS Biology"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Yan, Shuling"],["dc.contributor.author","Acharya, Sreemukta"],["dc.contributor.author","Gröning, Stephanie"],["dc.contributor.author","Großhans, Jörg"],["dc.date.accessioned","2019-02-13T12:35:26Z"],["dc.date.available","2019-02-13T12:35:26Z"],["dc.date.issued","2017"],["dc.description.abstract","Many mRNAs specifically localize within the cytoplasm and are present in RNA-protein complexes. It is generally assumed that localization and complex formation of these RNAs are controlled by trans-acting proteins encoded by genes different than the RNAs themselves. Here, we analyze slow as molasses (slam) mRNA that prominently colocalizes with its encoded protein at the basal cortical compartment during cellularization. The functional implications of this striking colocalization have been unknown. Here, we show that slam mRNA translation is spatiotemporally controlled. We found that translation was largely restricted to the onset of cellularization when Slam protein levels at the basal domain sharply increase. slam mRNA was translated locally, at least partially, as not yet translated mRNA transiently accumulated at the basal region. Slam RNA accumulated at the basal domain only if Slam protein was present. Furthermore, a slam RNA with impaired localization but full coding capacity was only weakly translated. We detected a biochemical interaction of slam mRNA and protein as demonstrated by specific co-immunoprecipitation from embryonic lysate. The intimate relationship of slam mRNA and protein may constitute a positive feedback loop that facilitates and controls timely and rapid accumulation of Slam protein at the prospective basal region."],["dc.identifier.doi","10.1371/journal.pbio.2003315"],["dc.identifier.pmid","29206227"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15662"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/57558"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation.eissn","1545-7885"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Slam protein dictates subcellular localization and translation of its own mRNA"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1112"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Integrative Biology"],["dc.bibliographiccitation.lastpage","1119"],["dc.bibliographiccitation.volume","3"],["dc.contributor.author","Kanesaki, Takuma"],["dc.contributor.author","Edwards, Carina M."],["dc.contributor.author","Schwarz, Ulrich S."],["dc.contributor.author","Grosshans, Joerg"],["dc.date.accessioned","2018-11-07T09:01:25Z"],["dc.date.available","2018-11-07T09:01:25Z"],["dc.date.issued","2011"],["dc.description.abstract","In syncytial embryos nuclei undergo cycles of division and rearrangement within a common cytoplasm. It is presently unclear to what degree and how the nuclear array maintains positional order in the face of rapid cell divisions. Here we establish a quantitative assay, based on image processing, for analysing the dynamics of the nuclear array. By tracking nuclear trajectories in Drosophila melanogaster embryos, we are able to define and evaluate local and time-dependent measures for the level of geometrical order in the array. We find that after division, order is re-established in a biphasic manner, indicating the competition of different ordering processes. Using mutants and drug injections, we show that the order of the nuclear array depends on cytoskeletal networks organised by centrosomes. While both f-actin and microtubules are required for re-establishing order after mitosis, only f-actin is required to maintain the stability of this arrangement. Furthermore, f-actin function relies on myosin-independent non-contractile filaments that suppress individual nuclear mobility, whereas microtubules promote mobility and attract adjacent nuclei. Actin caps are shown to act to prevent nuclear incorporation into adjacent microtubule baskets. Our data demonstrate that two principal ordering mechanisms thus simultaneously contribute: (1) a passive crowding mechanism in which nuclei and actin caps act as spacers and (2) an active self-organisation mechanism based on a microtubule network."],["dc.identifier.doi","10.1039/c1ib00059d"],["dc.identifier.isi","000296335000006"],["dc.identifier.pmid","22001900"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/8350"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24421"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Royal Soc Chemistry"],["dc.relation.issn","1757-9694"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Dynamic ordering of nuclei in syncytial embryos: a quantitative analysis of the role of cytoskeletal networks"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","208"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Developmental Biology"],["dc.bibliographiccitation.lastpage","220"],["dc.bibliographiccitation.volume","390"],["dc.contributor.author","Zhang, Yujun"],["dc.contributor.author","Kong, Deqing"],["dc.contributor.author","Reichl, Lars"],["dc.contributor.author","Vogt, Nina"],["dc.contributor.author","Wolf, Fred"],["dc.contributor.author","Großhans, Jörg"],["dc.date.accessioned","2017-09-07T11:45:40Z"],["dc.date.available","2017-09-07T11:45:40Z"],["dc.date.issued","2014"],["dc.description.abstract","The majority of membrane and secreted proteins, including many developmentally important signalling proteins, receptors and adhesion molecules, are cotranslationally N-glycosylated in the endoplasmic reticulum. The structure of the N-glycan is invariant for all substrates and conserved in eukaryotes. Correspondingly, the enzymes are conserved, which successively assemble the glycan precursor from activated monosaccharides prior to transfer to nascent proteins. Despite the well-defined biochemistry, the physiological and developmental role of N-glycosylation and of the responsible enzymes has not been much investigated in metazoa. We identified a mutation in the Drosophila gene, xiantuan (xit, CG4542), which encodes one of the conserved enzymes involved in addition of the terminal glucose residues to the glycan precursor. xit is required for timely apical constriction of mesoderm precursor cells and ventral furrow formation in early embryogenesis. Furthermore, cell intercalation in the lateral epidermis during germband extension is impaired in xit mutants. xit affects glycosylation and intracellular distribution of E-Cadherin, albeit not the total amount of E-Cadherin protein. As depletion of E-Cadherin by RNAi induces a similar cell intercalation defect, E-Cadherin may be the major xit target that is functionally relevant for germband extension."],["dc.identifier.doi","10.1016/j.ydbio.2014.03.007"],["dc.identifier.gro","3151839"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11361"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/8666"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","public"],["dc.notes.submitter","chake"],["dc.relation.issn","0012-1606"],["dc.rights","CC BY-NC-ND 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-nd/3.0"],["dc.title","The glucosyltransferase Xiantuan of the endoplasmic reticulum specifically affects E-Cadherin expression and is required for gastrulation movements in Drosophila"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e27634"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Communicative & Integrative Biology"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Bogdan, Sven"],["dc.contributor.author","Schultz, Jörg"],["dc.contributor.author","Grosshans, Jörg"],["dc.date.accessioned","2019-07-09T11:54:53Z"],["dc.date.available","2019-07-09T11:54:53Z"],["dc.date.issued","2013"],["dc.description.abstract","Members of the Diaphanous (Dia) protein family are key regulators of fundamental actin driven cellular processes, which are conserved from yeast to humans. Researchers have uncovered diverse physiological roles in cell morphology, cell motility, cell polarity, and cell division, which are involved in shaping cells into tissues and organs. The identification of numerous binding partners led to substantial progress in our understanding of the differential functions of Dia proteins. Genetic approaches and new microscopy techniques allow important new insights into their localization, activity, and molecular principles of regulation."],["dc.identifier.doi","10.4161/cib.27634"],["dc.identifier.fs","605839"],["dc.identifier.pmid","24719676"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9876"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60758"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1942-0889"],["dc.rights","CC BY-NC 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc/3.0"],["dc.title","Formin’ cellular structures: Physiological roles of Diaphanous (Dia) in actin dynamics"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2817"],["dc.bibliographiccitation.issue","21"],["dc.bibliographiccitation.journal","Cell Cycle"],["dc.bibliographiccitation.lastpage","2827"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Liu, Boyang"],["dc.contributor.author","Großhans, Jörg"],["dc.date.accessioned","2020-12-10T18:15:15Z"],["dc.date.available","2020-12-10T18:15:15Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1080/15384101.2019.1665948"],["dc.identifier.eissn","1551-4005"],["dc.identifier.issn","1538-4101"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/74790"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","The role of dNTP metabolites in control of the embryonic cell cycle"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1899"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","1907"],["dc.bibliographiccitation.volume","108"],["dc.contributor.author","Wessel, Alok D."],["dc.contributor.author","Gumalla, Maheshwar"],["dc.contributor.author","Großhans, Jörg"],["dc.contributor.author","Schmidt, Christoph"],["dc.date.accessioned","2017-09-07T11:44:27Z"],["dc.date.available","2017-09-07T11:44:27Z"],["dc.date.issued","2015"],["dc.description.abstract","In early development, Drosophila melanogaster embryos forma syncytium, i.e., multiplying nuclei are not yet separated by cell membranes, but are interconnected by cytoskeletal polymer networks consisting of actin and microtubules. Between division cycles 9 and 13, nuclei and cytoskeleton form a two-dimensional cortical layer. To probe the mechanical properties and dynamics of this self-organizing pre-tissue, we measured shear moduli in the embryo by high-speed video microrheology. We recorded position fluctuations of injected micron-sized fluorescent beads with kHz sampling frequencies and characterized the viscoelasticity of the embryo in different locations. Thermal fluctuations dominated over nonequilibrium activity for frequencies between 0.3 and 1000 Hz. Between the nuclear layer and the yolk, the cytoplasm was homogeneous and viscously dominated, with a viscosity three orders of magnitude higher than that of water. Within the nuclear layer we found an increase of the elastic and viscous moduli consistent with an increased microtubule density. Drug-interference experiments showedthat microtubules contribute to the measured viscoelasticity inside the embryo whereas actin only plays a minor role in the regions outside of the actin caps that are closely associated with the nuclei. Measurements at different stages of the nuclear division cycle showed little variation."],["dc.identifier.doi","10.1016/j.bpj.2015.02.032"],["dc.identifier.gro","3141922"],["dc.identifier.isi","000353344400009"],["dc.identifier.pmid","25902430"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2567"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Cell Press"],["dc.relation.eissn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.title","The Mechanical Properties of Early Drosophila Embryos Measured by High-Speed Video Microrheology"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1187"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Current Biology"],["dc.bibliographiccitation.lastpage","1192.e3"],["dc.bibliographiccitation.volume","29"],["dc.contributor.author","Liu, Boyang"],["dc.contributor.author","Winkler, Franziska"],["dc.contributor.author","Herde, Marco"],["dc.contributor.author","Witte, Claus-Peter"],["dc.contributor.author","Großhans, Jörg"],["dc.date.accessioned","2020-12-10T14:23:22Z"],["dc.date.available","2020-12-10T14:23:22Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1016/j.cub.2019.02.021"],["dc.identifier.issn","0960-9822"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71911"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","A Link between Deoxyribonucleotide Metabolites and Embryonic Cell-Cycle Control"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","191"],["dc.bibliographiccitation.issue","3-4"],["dc.bibliographiccitation.journal","Fly"],["dc.bibliographiccitation.lastpage","198"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Yan, Shuling"],["dc.contributor.author","Großhans, Jörg"],["dc.date.accessioned","2020-12-10T18:15:28Z"],["dc.date.available","2020-12-10T18:15:28Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1080/19336934.2018.1520574"],["dc.identifier.eissn","1933-6942"],["dc.identifier.issn","1933-6934"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/74855"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Localization and translation control of slam in Drosophila cellularization"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1796"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Journal of Cell Science"],["dc.bibliographiccitation.lastpage","1805"],["dc.bibliographiccitation.volume","126"],["dc.contributor.author","Yan, Shuling"],["dc.contributor.author","Lv, Zhiyi"],["dc.contributor.author","Winterhoff, Moritz"],["dc.contributor.author","Wenzl, Christian"],["dc.contributor.author","Zobel, Thomas"],["dc.contributor.author","Faix, Jan"],["dc.contributor.author","Bogdan, Sven"],["dc.contributor.author","Grosshans, Joerg"],["dc.date.accessioned","2018-11-07T09:25:57Z"],["dc.date.available","2018-11-07T09:25:57Z"],["dc.date.issued","2013"],["dc.description.abstract","During Drosophila embryogenesis, the first epithelium with defined cortical compartments is established during cellularization. Actin polymerization is required for the separation of lateral and basal domains as well as suppression of tubular extensions in the basal domain. The actin nucleator mediating this function is unknown. We found that the formin Diaphanous (Dia) is required for establishing and maintaining distinct lateral and basal domains during cellularization. In dia mutant embryos lateral marker proteins, such as Discs-large and Armadillo/beta-Catenin spread into the basal compartment. Furthermore, high-resolution and live-imaging analysis of dia mutant embryos revealed an increased number of membrane extensions and endocytic activity at the basal domain, indicating a suppressing function of dia on membrane invaginations. Dia function might be based on an antagonistic interaction with the F-BAR protein Cip4/Toca-1, a known activator of the WASP/WAVE-Arp2/3 pathway. Dia and Cip4 physically and functionally interact and overexpression of Cip4 phenocopies dia loss-of-function. In vitro, Cip4 inhibits mainly actin nucleation by Dia. Thus, our data support a model in which linear actin filaments induced by Dia stabilize cortical compartmentalization by antagonizing membrane turnover induced by WASP/WAVE-Arp2/3."],["dc.identifier.doi","10.1242/jcs.118422"],["dc.identifier.isi","000318975700009"],["dc.identifier.pmid","23424199"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/30186"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Company Of Biologists Ltd"],["dc.relation.issn","0021-9533"],["dc.title","The F-BAR protein Cip4/Toca-1 antagonizes the formin Diaphanous in membrane stabilization and compartmentalization"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2601"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","2608"],["dc.bibliographiccitation.volume","113"],["dc.contributor.author","Karsch, Susanne"],["dc.contributor.author","Kong, Deqing"],["dc.contributor.author","Großhans, Jörg"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2020-12-10T14:22:44Z"],["dc.date.available","2020-12-10T14:22:44Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1016/j.bpj.2017.10.025"],["dc.identifier.issn","0006-3495"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71713"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Single-Cell Defects Cause a Long-Range Mechanical Response in a Confluent Epithelial Cell Layer"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]