Chmyrov, Andriy

[["dc.bibliographiccitation.artnumber","527a"],["dc.bibliographiccitation.issue","6285"],["dc.bibliographiccitation.journal","Science"],["dc.bibliographiccitation.volume","352"],["dc.contributor.author","Sahl, Steffen J."],["dc.contributor.author","Balzarotti, Francisco"],["dc.contributor.author","Keller-Findeisen, Jan"],["dc.contributor.author","Leutenegger, Marcel"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Lavoie-Cardinal, Flavie"],["dc.contributor.author","Chmyrov, Andriy"],["dc.contributor.author","Grotjohann, Tim"],["dc.contributor.author","Jakobs, Stefan"],["dc.date.accessioned","2017-09-07T11:54:33Z"],["dc.date.available","2017-09-07T11:54:33Z"],["dc.date.issued","2016"],["dc.description.abstract","Li et al. (Research Articles, 28 August 2015, aab3500) purport to present solutions to longstanding challenges in live-cell microscopy, reporting relatively fast acquisition times in conjunction with improved image resolution. We question the methods' reliability to visualize specimen features at sub-100-nanometer scales, because the mandatory mathematical processing of the recorded data leads to artifacts that are either difficult or impossible to disentangle from real features. We are also concerned about the chosen approach of subjectively comparing images from different super-resolution methods, as opposed to using quantitative measures."],["dc.identifier.doi","10.1126/science.aad7983"],["dc.identifier.gro","3141696"],["dc.identifier.isi","000374998600028"],["dc.identifier.pmid","27126030"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1095-9203"],["dc.relation.issn","0036-8075"],["dc.title","Comment on \"Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics\""],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","letter_note"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","655"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","ChemPhysChem"],["dc.bibliographiccitation.lastpage","663"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Lavoie-Cardinal, Flavie"],["dc.contributor.author","Jensen, Nickels A."],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Stiel, Andre C."],["dc.contributor.author","Chmyrov, Andriy"],["dc.contributor.author","Bierwagen, Jakob"],["dc.contributor.author","Testa, Ilaria"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2017-09-07T11:46:25Z"],["dc.date.available","2017-09-07T11:46:25Z"],["dc.date.issued","2014"],["dc.description.abstract","Up to now, all demonstrations of reversible saturable optical fluorescence transitions (RESOLFT) superresolution microscopy of living cells have relied on the use of reversibly switchable fluorescent proteins (RSFP) emitting in the green spectral range. Here we show RESOLFT imaging with rsCherryRev1.4, a new red-emitting RSFP enabling a spatial resolution up to four times higher than the diffraction barrier. By co-expressing green and red RSFPs in living cells we demonstrate two-color RESOLFT imaging both for single (donut) beam scanning and for parallelized versions of RESOLFT nanoscopy where an array of >23000 donut-like minima are scanned simultaneously."],["dc.identifier.doi","10.1002/cphc.201301016"],["dc.identifier.gro","3142168"],["dc.identifier.isi","000332747500013"],["dc.identifier.pmid","24449030"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12826"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/5288"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Deutsche Forschungsgemeinschaft"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1439-7641"],["dc.relation.issn","1439-4235"],["dc.rights","CC BY-NC-ND 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-nd/3.0"],["dc.title","Two-Color RESOLFT Nanoscopy with Green and Red Fluorescent Photochromic Proteins"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","737"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Nature Methods"],["dc.bibliographiccitation.lastpage","742"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Chmyrov, Andriy"],["dc.contributor.author","Keller, Jan"],["dc.contributor.author","Grotjohann, Tim"],["dc.contributor.author","Ratz, Michael"],["dc.contributor.author","d'Este, Elisa"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2017-09-07T11:47:38Z"],["dc.date.available","2017-09-07T11:47:38Z"],["dc.date.issued","2013"],["dc.description.abstract","We show that nanoscopy based on the principle called RESOLFT (reversible saturable optical fluorescence transitions) or nonlinear structured illumination can be effectively parallelized using two incoherently superimposed orthogonal standing light waves. The intensity minima of the resulting pattern act as 'doughnuts', providing isotropic resolution in the focal plane and making pattern rotation redundant. We super-resolved living cells in 120 mm x 100 mm-sized fields of view in <1 s using 116,000 such doughnuts."],["dc.identifier.doi","10.1038/nmeth.2556"],["dc.identifier.gro","3142317"],["dc.identifier.isi","000322453600020"],["dc.identifier.pmid","23832150"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/6942"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1548-7105"],["dc.relation.issn","1548-7091"],["dc.title","Nanoscopy with more than 100,000 'doughnuts'"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","44619"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.lastpage","9"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Chmyrov, Andriy"],["dc.contributor.author","Leuschner, Ivo"],["dc.contributor.author","Grotjohann, Tim"],["dc.contributor.author","Schoenle, Andreas"],["dc.contributor.author","Keller-Findeisen, Jan"],["dc.contributor.author","Kastrup, Lars"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Donnert, Gerald"],["dc.contributor.author","Sahl, Steffen J."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2017-09-07T11:50:39Z"],["dc.date.available","2017-09-07T11:50:39Z"],["dc.date.issued","2017"],["dc.description.abstract","Fluorescence microscopy is rapidly turning into nanoscopy. Among the various nanoscopy methods, the STED/RESOLFT super-resolution family has recently been expanded to image even large fields of view within a few seconds. This advance relies on using light patterns featuring substantial arrays of intensity minima for discerning features by switching their fluorophores between ‘on’ and ‘off’ states of fluorescence. Here we show that splitting the light with a grating and recombining it in the focal plane of the objective lens renders arrays of minima with wavelength-independent periodicity. This colour-independent creation of periodic patterns facilitates coaligned on- and off-switching and readout with combinations chosen from a range of wavelengths. Applying up to three such periodic patterns on the switchable fluorescent proteins Dreiklang and rsCherryRev1.4, we demonstrate highly parallelized, multicolour RESOLFT nanoscopy in living cells for ~100 × 100 μm2 fields of view. Individual keratin filaments were rendered at a FWHM of ~60–80 nm, with effective resolution for the filaments of ~80–100 nm. We discuss the impact of novel image reconstruction algorithms featuring background elimination by spatial bandpass filtering, as well as strategies that incorporate complete image formation models."],["dc.identifier.doi","10.1038/srep44619"],["dc.identifier.gro","3145910"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14935"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/3644"],["dc.language.iso","en"],["dc.notes.intern","lifescience"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","chake"],["dc.relation.issn","2045-2322"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Achromatic light patterning and improved image reconstruction for parallelized RESOLFT nanoscopy"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]