Behr, Rüdiger

[["dc.bibliographiccitation.firstpage","2498"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Cells"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Stöckl, Jan B."],["dc.contributor.author","Schmid, Nina"],["dc.contributor.author","Flenkenthaler, Florian"],["dc.contributor.author","Drummer, Charis"],["dc.contributor.author","Behr, Rüdiger"],["dc.contributor.author","Mayerhofer, Artur"],["dc.contributor.author","Arnold, Georg J."],["dc.contributor.author","Fröhlich, Thomas"],["dc.date.accessioned","2022-10-06T13:26:49Z"],["dc.date.available","2022-10-06T13:26:49Z"],["dc.date.issued","2020"],["dc.description.abstract","Age-related changes in the human testis may include morphological alterations, disturbed steroidogenesis, and impaired spermatogenesis. However, the specific impact of cell age remains poorly understood and difficult to assess. Testicular peritubular cells fulfill essential functions, including sperm transport, contributions to the spermatogonial stem cell niche, and paracrine interactions within the testis. To study their role in age-associated decline of testicular functions, we performed comprehensive proteome and secretome analyses of repeatedly passaged peritubular cells from Callithrix jacchus. This nonhuman primate model better reflects the human testicular biology than rodents and further gives access to young donors unavailable from humans. Among 5095 identified proteins, 583 were differentially abundant between samples with low and high passage numbers. The alterations indicate a reduced ability of senescent peritubular cells to contract and secrete proteins, as well as disturbances in nuclear factor (NF)-κB signaling and a reduced capacity to handle reactive oxygen species. Since this in vitro model may not exactly mirror all molecular aspects of in vivo aging, we investigated the proteomes and secretomes of testicular peritubular cells from young and old donors. Even though the age-related alterations at the protein level were less pronounced, we found evidence for impaired protein secretion, altered NF-κB signaling, and reduced contractility of these in vivo aged peritubular cells."],["dc.identifier.doi","10.3390/cells9112498"],["dc.identifier.pii","cells9112498"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115175"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","2073-4409"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Proteomic Insights into Senescence of Testicular Peritubular Cells from a Nonhuman Primate Model"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3164"],["dc.bibliographiccitation.issue","19"],["dc.bibliographiccitation.journal","Cells"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Stepanov, Youli Konstantinovitch"],["dc.contributor.author","Speidel, Jan Dominik"],["dc.contributor.author","Herrmann, Carola"],["dc.contributor.author","Schmid, Nina"],["dc.contributor.author","Behr, Rüdiger"],["dc.contributor.author","Köhn, Frank-Michael"],["dc.contributor.author","Stöckl, Jan Bernd"],["dc.contributor.author","Pickl, Ulrich"],["dc.contributor.author","Trottmann, Matthias"],["dc.contributor.author","Fröhlich, Thomas"],["dc.contributor.author","Welter, Harald"],["dc.date.accessioned","2022-11-01T10:17:24Z"],["dc.date.available","2022-11-01T10:17:24Z"],["dc.date.issued","2022"],["dc.description.abstract","The functions of human testicular peritubular cells (HTPCs), forming a small compartment located between the seminiferous epithelium and the interstitial areas of the testis, are not fully known but go beyond intratesticular sperm transport and include immunological roles. The expression of the glucocorticoid receptor (GR) indicates that they may be regulated by glucocorticoids (GCs). Herein, we studied the consequences of the GC dexamethasone (Dex) in cultured HTPCs, which serves as a unique window into the human testis. We examined changes in cytokines, mainly by qPCR and ELISA. A holistic mass-spectrometry-based proteome analysis of cellular and secreted proteins was also performed. Dex, used in a therapeutic concentration, decreased the transcript level of proinflammatory cytokines, e.g., IL6, IL8 and MCP1. An siRNA-mediated knockdown of GR reduced the actions on IL6. Changes in IL6 were confirmed by ELISA measurements. Of note, Dex also lowered GR levels. The proteomic results revealed strong responses after 24 h (31 significantly altered cellular proteins) and more pronounced ones after 72 h of Dex exposure (30 less abundant and 42 more abundant cellular proteins). Dex also altered the composition of the secretome (33 proteins decreased, 13 increased) after 72 h. Among the regulated proteins were extracellular matrix (ECM) and basement membrane components (e.g., FBLN2, COL1A2 and COL3A1), as well as PTX3 and StAR. These results pinpoint novel, profound effects of Dex in HTPCs. If transferrable to the human testis, changes specifically in ECM and the immunological state of the testis may occur in men upon treatment with Dex for medical reasons."],["dc.identifier.doi","10.3390/cells11193164"],["dc.identifier.pii","cells11193164"],["dc.identifier.pmid","36231125"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/116801"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-605"],["dc.relation.eissn","2073-4409"],["dc.relation.issn","2073-4409"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Profound Effects of Dexamethasone on the Immunological State, Synthesis and Secretion Capacity of Human Testicular Peritubular Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","231"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Reproduction"],["dc.bibliographiccitation.lastpage","238"],["dc.bibliographiccitation.volume","156"],["dc.contributor.author","Walenta, Lena"],["dc.contributor.author","Schmid, Nina"],["dc.contributor.author","Schwarzer, J Ullrich"],["dc.contributor.author","Köhn, Frank-Michael"],["dc.contributor.author","Urbanski, Henryk F"],["dc.contributor.author","Behr, Rüdiger"],["dc.contributor.author","Strauss, Leena"],["dc.contributor.author","Poutanen, Matti"],["dc.contributor.author","Mayerhofer, Artur"],["dc.date.accessioned","2022-10-06T13:26:27Z"],["dc.date.available","2022-10-06T13:26:27Z"],["dc.date.issued","2018"],["dc.description.abstract","NLRP3 is part of the NLRP3 inflammasome and a global sensor of cellular damage. It was recently discovered in rodent Sertoli cells. We investigated NLRP3 in mouse, human and non-human primate (marmoset and rhesus macaque) testes, employing immunohistochemistry. Sertoli cells of all species expressed NLRP3, and the expression preceded puberty. In addition, peritubular cells of the adult human testes expressed NLRP3.\n NLRP3\n and associated genes (\n PYCARD\n ,\n CASP1\n ,\n IL1B\n ) were also found in isolated human testicular peritubular cells and the mouse Sertoli cell line TM4. Male infertility due to impairments of spermatogenesis may be related to sterile inflammatory events. We observed that the expression of NLRP3 was altered in the testes of patients suffering from mixed atrophy syndrome, in which tubules with impairments of spermatogenesis showed prominent NLRP3 staining. In order to explore a possible role of NLRP3 in male infertility, associated with sterile testicular inflammation, we studied a mouse model of male infertility. These human aromatase-expressing transgenic mice (\n AROM+\n ) develop testicular inflammation and impaired spermatogenesis during aging, and the present data show that this is associated with strikingly elevated\n Nlrp3\n expression in the testes compared to WT controls. Interference by aromatase inhibitor treatment significantly reduced increased\n Nlrp3\n levels. Thus, throughout species NLRP3 is expressed by somatic cells of the testis, which are involved in testicular immune surveillance. We conclude that NLRP3 may be a novel player in testicular immune regulation."],["dc.identifier.doi","10.1530/REP-18-0111"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115091"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1741-7899"],["dc.relation.issn","1470-1626"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","NLRP3 in somatic non-immune cells of rodent and primate testes"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Cell Death Discovery"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Bagnjuk, Konstantin"],["dc.contributor.author","Stöckl, Jan Bernd"],["dc.contributor.author","Fröhlich, Thomas"],["dc.contributor.author","Arnold, Georg Josef"],["dc.contributor.author","Behr, Rüdiger"],["dc.contributor.author","Berg, Ulrike"],["dc.contributor.author","Berg, Dieter"],["dc.contributor.author","Kunz, Lars"],["dc.contributor.author","Bishop, Cecily"],["dc.contributor.author","Xu, Jing"],["dc.contributor.author","Mayerhofer, Artur"],["dc.date.accessioned","2022-10-06T13:34:13Z"],["dc.date.available","2022-10-06T13:34:13Z"],["dc.date.issued","2019"],["dc.description.sponsorship"," Deutsche Forschungsgemeinschaft https://doi.org/10.13039/501100001659"],["dc.description.sponsorship"," U.S. Department of Health & Human Services | NIH | NIH Office of the Director https://doi.org/10.13039/100000052"],["dc.identifier.doi","10.1038/s41420-019-0149-7"],["dc.identifier.pii","149"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115859"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","2058-7716"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Necroptosis in primate luteolysis: a role for ceramide"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","721"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Molecular Human Reproduction"],["dc.bibliographiccitation.lastpage","727"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Gashaw, I."],["dc.contributor.author","Dushaj, O."],["dc.contributor.author","Behr, R."],["dc.contributor.author","Biermann, K."],["dc.contributor.author","Brehm, R."],["dc.contributor.author","Rubben, H."],["dc.contributor.author","Grobholz, R."],["dc.contributor.author","Schmid, K. W."],["dc.contributor.author","Bergmann, M."],["dc.contributor.author","Winterhager, E."],["dc.date.accessioned","2022-10-06T13:35:13Z"],["dc.date.available","2022-10-06T13:35:13Z"],["dc.date.issued","2007"],["dc.identifier.doi","10.1093/molehr/gam059"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/116044"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1460-2407"],["dc.relation.issn","1360-9947"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Novel germ cell markers characterize testicular seminoma and fetal testis"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","1011109"],["dc.bibliographiccitation.journal","Frontiers in Cell and Developmental Biology"],["dc.bibliographiccitation.volume","10"],["dc.contributor.affiliation","Nguyen, Huong; \r\n1\r\nInstitute for Neuroanatomy, University Medical Center, Georg-August-University Goettingen, Goettingen, Germany"],["dc.contributor.affiliation","Sokpor, Godwin; \r\n1\r\nInstitute for Neuroanatomy, University Medical Center, Georg-August-University Goettingen, Goettingen, Germany"],["dc.contributor.affiliation","Parichha, Arpan; \r\n5\r\nTata Institute of Fundamental Research, Mumbai, India"],["dc.contributor.affiliation","Pham, Linh; \r\n1\r\nInstitute for Neuroanatomy, University Medical Center, Georg-August-University Goettingen, Goettingen, Germany"],["dc.contributor.affiliation","Saikhedkar, Nidhi; \r\n5\r\nTata Institute of Fundamental Research, Mumbai, India"],["dc.contributor.affiliation","Xie, Yuanbin; \r\n1\r\nInstitute for Neuroanatomy, University Medical Center, Georg-August-University Goettingen, Goettingen, Germany"],["dc.contributor.affiliation","Ulmke, Pauline Antonie; \r\n1\r\nInstitute for Neuroanatomy, University Medical Center, Georg-August-University Goettingen, Goettingen, Germany"],["dc.contributor.affiliation","Rosenbusch, Joachim; \r\n1\r\nInstitute for Neuroanatomy, University Medical Center, Georg-August-University Goettingen, Goettingen, Germany"],["dc.contributor.affiliation","Pirouz, Mehdi; \r\n6\r\nMax Planck Institute for Multidisciplinary Sciences, Goettingen, Germany"],["dc.contributor.affiliation","Behr, Rüdiger; \r\n8\r\nGerman Primate Center-Leibniz Institute for Primate Research, Goettingen, Germany"],["dc.contributor.affiliation","Stoykova, Anastassia; \r\n6\r\nMax Planck Institute for Multidisciplinary Sciences, Goettingen, Germany"],["dc.contributor.affiliation","Brand-Saberi, Beate; \r\n4\r\nDepartment of Anatomy and Molecular Embryology, Ruhr University Bochum, Bochum, Germany"],["dc.contributor.affiliation","Nguyen, Huu Phuc; \r\n3\r\nDepartment of Human Genetics, Ruhr University Bochum, Bochum, Germany"],["dc.contributor.affiliation","Staiger, Jochen F.; \r\n1\r\nInstitute for Neuroanatomy, University Medical Center, Georg-August-University Goettingen, Goettingen, Germany"],["dc.contributor.affiliation","Tole, Shubha; \r\n5\r\nTata Institute of Fundamental Research, Mumbai, India"],["dc.contributor.affiliation","Tuoc, Tran; \r\n1\r\nInstitute for Neuroanatomy, University Medical Center, Georg-August-University Goettingen, Goettingen, Germany"],["dc.contributor.author","Nguyen, Huong"],["dc.contributor.author","Sokpor, Godwin"],["dc.contributor.author","Parichha, Arpan"],["dc.contributor.author","Pham, Linh"],["dc.contributor.author","Saikhedkar, Nidhi"],["dc.contributor.author","Xie, Yuanbin"],["dc.contributor.author","Ulmke, Pauline Antonie"],["dc.contributor.author","Rosenbusch, Joachim"],["dc.contributor.author","Pirouz, Mehdi"],["dc.contributor.author","Behr, Rüdiger"],["dc.contributor.author","Tuoc, Tran"],["dc.contributor.author","Stoykova, Anastassia"],["dc.contributor.author","Brand-Saberi, Beate"],["dc.contributor.author","Nguyen, Huu Phuc"],["dc.contributor.author","Staiger, Jochen F."],["dc.contributor.author","Tole, Shubha"],["dc.date.accessioned","2022-11-01T10:17:17Z"],["dc.date.available","2022-11-01T10:17:17Z"],["dc.date.issued","2022"],["dc.date.updated","2022-11-11T13:12:49Z"],["dc.description.abstract","Early forebrain patterning entails the correct regional designation of the neuroepithelium, and appropriate specification, generation, and distribution of neural cells during brain development. Specific signaling and transcription factors are known to tightly regulate patterning of the dorsal telencephalon to afford proper structural/functional cortical arealization and morphogenesis. Nevertheless, whether and how changes of the chromatin structure link to the transcriptional program(s) that control cortical patterning remains elusive. Here, we report that the BAF chromatin remodeling complex regulates the spatiotemporal patterning of the mouse dorsal telencephalon. To determine whether and how the BAF complex regulates cortical patterning, we conditionally deleted the BAF complex scaffolding subunits BAF155 and BAF170 in the mouse dorsal telencephalic neuroepithelium. Morphological and cellular changes in the BAF mutant forebrain were examined using immunohistochemistry and\r\n in situ\r\n hybridization. RNA sequencing, Co-immunoprecipitation, and mass spectrometry were used to investigate the molecular basis of BAF complex involvement in forebrain patterning. We found that conditional ablation of BAF complex in the dorsal telencephalon neuroepithelium caused expansion of the cortical hem and medial cortex beyond their developmental boundaries. Consequently, the hippocampal primordium is not specified, the mediolateral cortical patterning is compromised, and the cortical identity is disturbed in the absence of BAF complex. The BAF complex was found to interact with the cortical hem suppressor LHX2. The BAF complex suppresses cortical hem fate to permit proper forebrain patterning. We provide evidence that BAF complex modulates mediolateral cortical patterning possibly by interacting with the transcription factor LHX2 to drive the LHX2-dependent transcriptional program essential for dorsal telencephalon patterning. Our data suggest a putative mechanistic synergy between BAF chromatin remodeling complex and LHX2 in regulating forebrain patterning and ontogeny."],["dc.description.sponsorship"," Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659"],["dc.description.sponsorship"," National Institute of Diabetes and Digestive and Kidney Diseases http://dx.doi.org/10.13039/100000062"],["dc.identifier.doi","10.3389/fcell.2022.1011109"],["dc.identifier.pmid","36263009"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/116773"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-605"],["dc.publisher","Frontiers Media S.A."],["dc.relation.eissn","2296-634X"],["dc.relation.issn","2296-634X"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.title","BAF (mSWI/SNF) complex regulates mediolateral cortical patterning in the developing forebrain"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","733"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Reproduction"],["dc.bibliographiccitation.lastpage","742"],["dc.bibliographiccitation.volume","140"],["dc.contributor.author","Albert, S"],["dc.contributor.author","Ehmcke, J"],["dc.contributor.author","Wistuba, J"],["dc.contributor.author","Eildermann, K"],["dc.contributor.author","Behr, R"],["dc.contributor.author","Schlatt, S"],["dc.contributor.author","Gromoll, J"],["dc.date.accessioned","2022-10-06T13:26:26Z"],["dc.date.available","2022-10-06T13:26:26Z"],["dc.date.issued","2010"],["dc.description.abstract","The seminiferous epithelium in the nonhuman primate\r\n Callithrix jacchus\r\n is similarly organized to man. This monkey has therefore been used as a preclinical model for spermatogenesis and testicular stem cell physiology. However, little is known about the developmental dynamics of germ cells in the postnatal primate testis. In this study, we analyzed testes of newborn, 8-week-old, and adult marmosets employing immunohistochemistry using pluripotent stem cell and germ cell markers\r\n DDX4\r\n (\r\n VASA\r\n ),\r\n POU5F1\r\n (\r\n OCT3/4\r\n ), and\r\n TFAP2C\r\n (\r\n AP-2\r\n γ\r\n ). Stereological and morphometric techniques were applied for quantitative analysis of germ cell populations and testicular histological changes. Quantitative RT-PCR (qRT-PCR) of testicular mRNA was applied using 16 marker genes establishing the corresponding profiles during postnatal testicular development. Testis size increased during the first 8 weeks of life with the main driver being longitudinal outgrowth of seminiferous cords. The number of DDX4-positive cells per testis doubled between birth and 8 weeks of age whereas TFAP2C- and POU5F1-positive cells remained unchanged. This increase in DDX4-expressing cells indicates dynamic growth of the differentiated A-spermatogonial population. The presence of cells expressing POU5F1 and TFAP2C after 8 weeks reveals the persistence of less differentiated germ cells. The mRNA and protein profiles determined by qRT-PCR and western blot in newborn, 8-week-old, and adult marmosets corroborated the immunohistochemical findings. In conclusion, we demonstrated the presence of distinct spermatogonial subpopulations in the primate testis exhibiting different dynamics during early testicular development. Our study demonstrates the suitability of the marmoset testis as a model for human testicular development."],["dc.identifier.doi","10.1530/REP-10-0235"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115088"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1741-7899"],["dc.relation.issn","1470-1626"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Germ cell dynamics in the testis of the postnatal common marmoset monkey (Callithrix jacchus)"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","101"],["dc.bibliographiccitation.journal","Reproduction"],["dc.bibliographiccitation.lastpage","109"],["dc.contributor.author","Wolff, E"],["dc.contributor.author","Suplicki, M M"],["dc.contributor.author","Behr, R"],["dc.date.accessioned","2022-10-06T13:26:27Z"],["dc.date.available","2022-10-06T13:26:27Z"],["dc.date.issued","2019"],["dc.description.abstract","Primordial germ cells (PGCs) are the embryonic precursors of spermatozoa and eggs. In mammals, PGCs arise early in embryonic development and migrate from their tissue of specification over a significant distance to reach their destinations, the genital ridges. However, the exact mechanism of translocation is still debated. A study on human embryos demonstrated a very close spatial association between migrating PGCs and developing peripheral nerves. Thus, it was proposed that peripheral nerves act as guiding structures for migrating PGCs. The goal of the present study is to test whether the association between nerves and PGCs may be a human-specific finding or whether this represents a general strategy to guide PGCs in mammals. Therefore, we investigated embryos of different developmental stages from the mouse and a non-human primate, the marmoset monkey (\n Callithrix jacchus\n ), covering the phase from PGC emergence to their arrival in the gonadal ridge. Embryo sections were immunohistochemically co-stained for tubulin beta-3 chain (TUBB3) to visualise neurons and Octamer-binding protein 4 (OCT4 (POU5F1)) as marker for PGCs. The distance between PGCs and the nearest detectable neuron was measured. We discovered that in all embryos analysed of both species, the majority of PGCs (>94%) was found at a minimum distance of 50 µm to the closest neuron and, more importantly, that the PGCs had reached the gonads before any TUBB3 signal could be detected in the vicinity of the gonads. In conclusion, our data indicate that PGC migration along peripheral nerves is not a general mechanism in mammals."],["dc.identifier.doi","10.1530/REP-18-0401"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115092"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1741-7899"],["dc.relation.issn","1470-1626"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Primordial germ cells do not migrate along nerve fibres in marmoset monkey and mouse embryos"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","300"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Reproduction, Fertility and Development"],["dc.bibliographiccitation.volume","25"],["dc.contributor.author","Talluri, T. R."],["dc.contributor.author","Hermann, D."],["dc.contributor.author","Barg-Kues, B."],["dc.contributor.author","Debowski, K."],["dc.contributor.author","Behr, R."],["dc.contributor.author","Ivics, Z."],["dc.contributor.author","Hall, V. J."],["dc.contributor.author","Rasmussen, M. A."],["dc.contributor.author","Hyttel, P."],["dc.contributor.author","Niemann, H."],["dc.contributor.author","Kues, W. A."],["dc.date.accessioned","2022-10-06T13:34:30Z"],["dc.date.available","2022-10-06T13:34:30Z"],["dc.date.issued","2013"],["dc.description.abstract","The elusive nature of embryonic stem cells in livestock makes reprogramming of somatic cells to induced pluripotent stem (iPS) cells a promising approach for targeted genetic modifications. The first attempts to produce iPS cells from livestock species were made using retro- and lentiviral vectors, which are associated with an increased risk of insertional mutagenesis and which are not easily removable after reprogramming. Here, we describe a nonviral method for the derivation of porcine and bovine iPS cells, using Sleeping Beauty (SB) and piggyBac (PB) transposon systems. The transposons encode the murine or primate reprogramming factors OCT4, SOX2, KLF4, MYC, and LIN28, separated by self-cleaving peptide sequences, respectively. In addition, the PB transposon cassette contains a NANOG-cDNA. The SB or PB transposon-reprogrammed porcine iPS cells expressed typical markers of embryonic stem cells (SSEA1, SSEA4, TRA-1-60, and endogenous stemness genes), showed long-term proliferation under feeder-free culture conditions, differentiated into cell types of the 3 germ layers in vitro, and formed teratomas after subcutaneous injection into immune-deficient nude mice. Both transposon systems are currently being tested in bovine fibroblasts. The results are a major step towards the derivation of authentic porcine and bovine iPS cells, in which the transposon transgenes can be eliminated after reprogramming."],["dc.identifier.doi","10.1071/RDv25n1Ab305"],["dc.identifier.pii","RDv25n1Ab305"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115924"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.issn","1031-3613"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","305 Transposon-Mediated Reprogramming of Livestock Somatic Cells to Induced Pluripotent Stem Cells"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","599"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Histochemistry and Cell Biology"],["dc.bibliographiccitation.lastpage","609"],["dc.bibliographiccitation.volume","143"],["dc.contributor.author","Günther, Katharina"],["dc.contributor.author","Paradowska-Dogan, Agnieszka"],["dc.contributor.author","Bärmann, Birte"],["dc.contributor.author","Klein, Harald"],["dc.contributor.author","von Eichel-Streiber, Christoph"],["dc.contributor.author","Hartley, Ricardo"],["dc.contributor.author","Weidner, Wolfgang"],["dc.contributor.author","Behr, Rüdiger"],["dc.contributor.author","Steger, Klaus"],["dc.date.accessioned","2022-10-06T13:32:16Z"],["dc.date.available","2022-10-06T13:32:16Z"],["dc.date.issued","2015"],["dc.identifier.doi","10.1007/s00418-015-1309-3"],["dc.identifier.pii","1309"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115330"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1432-119X"],["dc.relation.issn","0948-6143"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.rights.uri","http://www.springer.com/tdm"],["dc.title","Expression of sperm-specific protamines impairs bacterial and eukaryotic cell proliferation"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]