Völker, Uwe

[["dc.bibliographiccitation.firstpage","171"],["dc.bibliographiccitation.journal","Metabolic Engineering"],["dc.bibliographiccitation.lastpage","179"],["dc.bibliographiccitation.volume","45"],["dc.contributor.author","Reuß, Daniel R."],["dc.contributor.author","Rath, Hermann"],["dc.contributor.author","Thürmer, Andrea"],["dc.contributor.author","Benda, Martin"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Völker, Uwe"],["dc.contributor.author","Mäder, Ulrike"],["dc.contributor.author","Commichau, Fabian M."],["dc.contributor.author","Stülke, Jörg"],["dc.date.accessioned","2020-12-10T15:21:49Z"],["dc.date.available","2020-12-10T15:21:49Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1016/j.ymben.2017.12.004"],["dc.identifier.issn","1096-7176"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/73172"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Changes of DNA topology affect the global transcription landscape and allow rapid growth of a Bacillus subtilis mutant lacking carbon catabolite repression"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e1009092"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","PLoS Genetics"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Krüger, Larissa"],["dc.contributor.author","Herzberg, Christina"],["dc.contributor.author","Rath, Hermann"],["dc.contributor.author","Pedreira, Tiago"],["dc.contributor.author","Ischebeck, Till"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Gundlach, Jan"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Völker, Uwe"],["dc.contributor.author","Mäder, Ulrike"],["dc.contributor.author","Stülke, Jörg"],["dc.date.accessioned","2021-04-14T08:29:55Z"],["dc.date.available","2021-04-14T08:29:55Z"],["dc.date.issued","2021"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.1371/journal.pgen.1009092"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83034"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1553-7404"],["dc.rights","CC BY 4.0"],["dc.title","Essentiality of c-di-AMP in Bacillus subtilis: Bypassing mutations converge in potassium and glutamate homeostasis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3914"],["dc.bibliographiccitation.issue","19"],["dc.bibliographiccitation.journal","Proteomics"],["dc.bibliographiccitation.lastpage","3927"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Kolata, Julia"],["dc.contributor.author","Bode, Lonneke G. M."],["dc.contributor.author","Holtfreter, Silva"],["dc.contributor.author","Steil, Leif"],["dc.contributor.author","Kusch, Harald"],["dc.contributor.author","Holtfreter, Birte"],["dc.contributor.author","Albrecht, Dirk"],["dc.contributor.author","Hecker, Michael"],["dc.contributor.author","Engelmann, Susanne"],["dc.contributor.author","van Belkum, Alex"],["dc.contributor.author","Volker, Uwe"],["dc.contributor.author","Bröker, Barbara M."],["dc.date.accessioned","2019-08-06T10:50:27Z"],["dc.date.available","2019-08-06T10:50:27Z"],["dc.date.issued","2011"],["dc.description.abstract","Staphylococcus aureus is both a prominent cause of nosocomial infections with significant morbidity and mortality and a commensal with nasal carriage in around 30% of the population. The rapid spread of multi-resistant strains necessitates novel therapeutic strategies, a challenging task because the species S. aureus and the host response against it are highly variable. In a prospective study among 2023 surgical and non-surgical patients, 12 patients developed S. aureus bacteremia. They were analysed in detail using a personalized approach. For each patient, the extracellular proteins of the infecting S. aureus strain were identified and the developing antibody response was assessed on 2-D immunoblots. S. aureus carriers showed clear evidence of strain-specific pre-immunization. In all immune-competent bacteremia patients, antibody binding increased strongly, in most cases already at diagnosis. In endogenous infections, the pattern of antibody binding was similar to the pre-infection pattern. In exogenous infections, in contrast, the pre-infection pattern was radically altered with the acquisition of new specificities. These were characteristic for individual patients. Nevertheless, a common signature of 11 conserved S. aureus proteins, recognized in at least half of the bacteremic patients, was identified. All patients mounted a dynamic antibody response to a subset of these proteins."],["dc.identifier.doi","10.1002/pmic.201000760"],["dc.identifier.pmid","21805632"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62303"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1615-9861"],["dc.relation.issn","1615-9853"],["dc.title","Distinctive patterns in the human antibody response to Staphylococcus aureus bacteremia in carriers and non-carriers"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e96875"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","PLOS ONE"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Schwedhelm, Edzard"],["dc.contributor.author","Wallaschofski, Henri"],["dc.contributor.author","Atzler, Dorothee"],["dc.contributor.author","Dörr, Marcus"],["dc.contributor.author","Nauck, Matthias"],["dc.contributor.author","Volker, Uwe"],["dc.contributor.author","Kroemer, Heyo K."],["dc.contributor.author","Volzke, Henry"],["dc.contributor.author","Böger, Rainer H."],["dc.contributor.author","Friedrich, Nele"],["dc.date.accessioned","2018-08-15T06:50:42Z"],["dc.date.available","2018-08-15T06:50:42Z"],["dc.date.issued","2014"],["dc.description.abstract","BACKGROUND: L-Arginine and its dimethylated derivatives asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) have been associated with cardiovascular (CV) and all-cause mortality in populations at risk. The present study aimed to investigate the prognostic value of L-arginine and its derivatives in the general population. METHODS AND RESULTS: We evaluated 3,952 individuals (1,936 men and 2,016 women) aged 20-81 (median (IQR) 51 (37; 64) years) from the population-based Study of Health in Pomerania (SHIP). Associations of continuous [per standard deviation (SD) increase] and categorized (age- and sex-specific tertiles) serum L-arginine, ADMA, and SDMA concentrations with all-cause and cause-specific mortality were analysed. During a median (IQR) follow-up period of 10.1 (9.3; 10.8) years (38,476 person-years), 426 deaths (10.8%) were observed, including 139 CV deaths (3.5%), and 150 cancer deaths (3.8%). After multivariable adjustment, we revealed a positive association of SDMA with all-cause [hazard ratio (HR) per SD increase: 1.16, 95% confidence interval (CI): 1.07-1.25] and CV mortality [HR: 1.19, 95% CI: 1.05-1.35]. In contrast, we did not observe any association of SDMA with cancer mortality. Neither L-arginine nor ADMA were associated with all-cause or CV mortality. CONCLUSION: SDMA, but not ADMA, is an independent predictor of all-cause and CV mortality in a large population-based cohort of European ancestry."],["dc.identifier.doi","10.1371/journal.pone.0096875"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10120"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/15305"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation.eissn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Incidence of all-cause and cardiovascular mortality predicted by symmetric dimethylarginine in the population-based study of health in pomerania"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1607"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Clinical and Vaccine Immunology"],["dc.bibliographiccitation.lastpage","1614"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Kusch, Harald"],["dc.contributor.author","Hecker, Michael"],["dc.contributor.author","Völker, Uwe"],["dc.contributor.author","Nguyen, TT"],["dc.contributor.author","Steil, Leif"],["dc.contributor.author","Bröker, Barbara M."],["dc.contributor.author","van Belkum, Alexander"],["dc.contributor.author","Holtfreter, Silva"],["dc.contributor.author","Wertheim, H"],["dc.contributor.author","Truong, QP"],["dc.contributor.author","Engelmann, Susanne"],["dc.creator.author","van Belkum A"],["dc.creator.author","Engelmann S"],["dc.creator.author","Nguyen TT"],["dc.creator.author","Wertheim H"],["dc.creator.author","Steil L"],["dc.creator.author","Truong QP"],["dc.creator.author","Bröker BM"],["dc.creator.author","Holtfreter S"],["dc.creator.author","Völker U"],["dc.creator.author","Hecker M"],["dc.creator.author","Kusch H"],["dc.date.accessioned","2019-10-09T09:43:16Z"],["dc.date.available","2019-10-09T09:43:16Z"],["dc.date.issued","2009"],["dc.identifier.doi","10.1128/CVI.00263-09"],["dc.identifier.pmid","19759252"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62480"],["dc.language.iso","en"],["dc.title","Human immune proteome in experimental colonization with Staphylococcus aureus"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","18"],["dc.bibliographiccitation.journal","Applied and Environmental Microbiology"],["dc.bibliographiccitation.volume","87"],["dc.contributor.author","van Tilburg, Amanda Y."],["dc.contributor.author","Fülleborn, Julius A."],["dc.contributor.author","Reder, Alexander"],["dc.contributor.author","Völker, Uwe"],["dc.contributor.author","Stülke, Jörg"],["dc.contributor.author","van Heel, Auke J."],["dc.contributor.author","Kuipers, Oscar P."],["dc.contributor.editor","Kivisaar, Maia"],["dc.date.accessioned","2021-10-01T09:58:04Z"],["dc.date.available","2021-10-01T09:58:04Z"],["dc.date.issued","2021"],["dc.description.abstract","Reduction of the size of bacterial genomes is relevant for understanding the minimal requirements for cellular life as well as from a biotechnological point of view. Although the genome-minimized Bacillus subtilis strain PG10 displays several beneficial traits as a microbial cell factory compared to its parental strain, a defect at the final stage of cell division was introduced during the genome reduction process."],["dc.description.abstract","ABSTRACT Cell chaining in Bacillus subtilis is naturally observed in a subset of cells during exponential growth and during biofilm formation. However, the recently constructed large-scale genome-minimized B. subtilis strain PG10 displays a severe and permanent defect in cell separation, as it exclusively grows in the form of long filaments of nonseparated cells. In this study, we investigated the underlying mechanisms responsible for the incomplete cell division of PG10 by genomic and transcriptomic analyses. Repression of the SigD regulon, including the major autolysin gene lytF , was identified as the cause for the cell separation problem of PG10. It appeared that SigD-regulated genes are downregulated in PG10 due to the absence of the flagellar export apparatus, which normally is responsible for secretion of FlgM, the anti-sigma factor of SigD. Although mild negative effects on growth and cell morphology were observed, deletion of flgM could revert the aberrant cell-chaining phenotype and increased transformation efficiency. Interestingly, our work also demonstrates the occurrence of increased antisense transcription of slrR , a transcriptional repressor of autolysin genes, in PG10 and provides further understanding for this observation. In addition to revealing the molecular basis of the cell separation defect in PG10, our work provides novel targets for subsequent genome reduction efforts and future directions for further optimization of mini Bacillus PG10. IMPORTANCE Reduction of the size of bacterial genomes is relevant for understanding the minimal requirements for cellular life as well as from a biotechnological point of view. Although the genome-minimized Bacillus subtilis strain PG10 displays several beneficial traits as a microbial cell factory compared to its parental strain, a defect at the final stage of cell division was introduced during the genome reduction process. By genetic and transcriptomic analyses, we identified the underlying reasons for the cell separation problem of PG10. In addition to enabling PG10 to grow in a way similar to that of B. subtilis wild-type strains, our work points toward subsequent targets for fine-tuning and further reduction of the genome of PG10. Moreover, solving the cell separation defect facilitates laboratory handling of PG10 by increasing the transformation efficiency, among other means. Overall, our work contributes to understanding and improving biotechnologically attractive minimal bacterial cell factories."],["dc.description.abstract","Reduction of the size of bacterial genomes is relevant for understanding the minimal requirements for cellular life as well as from a biotechnological point of view. Although the genome-minimized Bacillus subtilis strain PG10 displays several beneficial traits as a microbial cell factory compared to its parental strain, a defect at the final stage of cell division was introduced during the genome reduction process."],["dc.description.abstract","ABSTRACT Cell chaining in Bacillus subtilis is naturally observed in a subset of cells during exponential growth and during biofilm formation. However, the recently constructed large-scale genome-minimized B. subtilis strain PG10 displays a severe and permanent defect in cell separation, as it exclusively grows in the form of long filaments of nonseparated cells. In this study, we investigated the underlying mechanisms responsible for the incomplete cell division of PG10 by genomic and transcriptomic analyses. Repression of the SigD regulon, including the major autolysin gene lytF , was identified as the cause for the cell separation problem of PG10. It appeared that SigD-regulated genes are downregulated in PG10 due to the absence of the flagellar export apparatus, which normally is responsible for secretion of FlgM, the anti-sigma factor of SigD. Although mild negative effects on growth and cell morphology were observed, deletion of flgM could revert the aberrant cell-chaining phenotype and increased transformation efficiency. Interestingly, our work also demonstrates the occurrence of increased antisense transcription of slrR , a transcriptional repressor of autolysin genes, in PG10 and provides further understanding for this observation. In addition to revealing the molecular basis of the cell separation defect in PG10, our work provides novel targets for subsequent genome reduction efforts and future directions for further optimization of mini Bacillus PG10. IMPORTANCE Reduction of the size of bacterial genomes is relevant for understanding the minimal requirements for cellular life as well as from a biotechnological point of view. Although the genome-minimized Bacillus subtilis strain PG10 displays several beneficial traits as a microbial cell factory compared to its parental strain, a defect at the final stage of cell division was introduced during the genome reduction process. By genetic and transcriptomic analyses, we identified the underlying reasons for the cell separation problem of PG10. In addition to enabling PG10 to grow in a way similar to that of B. subtilis wild-type strains, our work points toward subsequent targets for fine-tuning and further reduction of the genome of PG10. Moreover, solving the cell separation defect facilitates laboratory handling of PG10 by increasing the transformation efficiency, among other means. Overall, our work contributes to understanding and improving biotechnologically attractive minimal bacterial cell factories."],["dc.identifier.doi","10.1128/AEM.01123-21"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/89981"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-469"],["dc.relation.eissn","1098-5336"],["dc.relation.issn","0099-2240"],["dc.title","Unchaining mini Bacillus Strain PG10: Relief of FlgM-Mediated Repression of Autolysin Genes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1798"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Journal of Clinical Investigation"],["dc.bibliographiccitation.lastpage","1812"],["dc.bibliographiccitation.volume","127"],["dc.contributor.author","Wild, Philipp S."],["dc.contributor.author","Felix, Janine F."],["dc.contributor.author","Schillert, Arne"],["dc.contributor.author","Teumer, Alexander"],["dc.contributor.author","Chen, Ming-Huei"],["dc.contributor.author","Leening, Maarten J.G."],["dc.contributor.author","Völker, Uwe"],["dc.contributor.author","Großmann, Vera"],["dc.contributor.author","Brody, Jennifer A."],["dc.contributor.author","Irvin, Marguerite R."],["dc.contributor.author","Dörr, Marcus"],["dc.date.accessioned","2020-12-10T18:38:18Z"],["dc.date.available","2020-12-10T18:38:18Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1172/JCI84840"],["dc.identifier.eissn","1558-8238"],["dc.identifier.issn","0021-9738"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/77267"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Large-scale genome-wide analysis identifies genetic variants associated with cardiac structure and function"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]