Heidenreich, Doris

[["dc.bibliographiccitation.journal","PLoS currents"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.contributor.author","Patel, Pranav"],["dc.contributor.author","Heidenreich, Doris"],["dc.contributor.author","Hufert, Frank T."],["dc.contributor.author","Weidmann, Manfred"],["dc.date.accessioned","2019-07-09T11:40:12Z"],["dc.date.available","2019-07-09T11:40:12Z"],["dc.date.issued","2013"],["dc.description.abstract","The emergence of Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in the eastern Mediterranean and imported cases to Europe has alerted public health authorities. Currently, detection of MERS-CoV in patient samples is done by real-time RT-PCR. Samples collected from suspected cases are sent to highly-equipped centralized laboratories for screening. A rapid point-of-care test is needed to allow more widespread mobile detection of the virus directly from patient material. In this study, we describe the development of a reverse transcription isothermal Recombinase Polymerase Amplification (RT-RPA) assay for the identification of MERS-CoV. A partial nucleocapsid gene RNA molecular standard of MERS-coronavirus was used to determine the assay sensitivity. The isothermal (42°C) MERS-CoV RT-RPA was as sensitive as real-time RT-PCR (10 RNA molecules), rapid (3-7 minutes) and mobile (using tubescanner weighing 1kg). The MERS-CoV RT-RPA showed cross-detection neither of any of the RNAs of several coronaviruses and respiratory viruses affecting humans nor of the human genome. The developed isothermal real-time RT-RPA is ideal for rapid mobile molecular MERS-CoV monitoring in acute patients and may also facilitate the search for the animal reservoir of MERS-CoV."],["dc.identifier.doi","10.1371/currents.outbreaks.62df1c7c75ffc96cd59034531e2e8364"],["dc.identifier.fs","601231"],["dc.identifier.pmid","24459611"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10752"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58113"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2157-3999"],["dc.rights","CC BY 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/3.0"],["dc.title","Reverse transcription recombinase polymerase amplification assay for the detection of middle East respiratory syndrome coronavirus."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0129682"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.contributor.author","Patel, Pranav"],["dc.contributor.author","Faye, Oumar"],["dc.contributor.author","Thaloengsok, Sasikanya"],["dc.contributor.author","Heidenreich, Doris"],["dc.contributor.author","Matangkasombut, Ponpan"],["dc.contributor.author","Manopwisedjaroen, Khajohnpong"],["dc.contributor.author","Sakuntabhai, Anavaj"],["dc.contributor.author","Sall, Amadou Alpha"],["dc.contributor.author","Hufert, Frank T."],["dc.contributor.author","Weidmann, Manfred"],["dc.date.accessioned","2018-11-07T09:55:51Z"],["dc.date.available","2018-11-07T09:55:51Z"],["dc.date.issued","2015"],["dc.description.abstract","Background Over 2.5 billion people are exposed to the risk of contracting dengue fever (DF). Early diagnosis of DF helps to diminish its burden on public health. Real-time reverse transcription polymerase amplification assays (RT-PCR) are the standard method for molecular detection of the dengue virus (DENV). Real-time RT-PCR analysis is not suitable for on-site screening since mobile devices are large, expensive, and complex. In this study, two RT-recombinase polymerase amplification (RT-RPA) assays were developed to detect DENV1-4. Methodology/Principal Findings Using two quantitative RNA molecular standards, the analytical sensitivity of a RT-RPA targeting the 3'non-translated region of DENV1-4 was found to range from 14 (DENV4) to 241 (DENV1-3) RNA molecules detected. The assay was specific and did not cross detect other Flaviviruses. The RT-RPA assay was tested in a mobile laboratory combining magneticbead based total nucleic acid extraction and a portable detection device in Kedougou (Senegal) and in Bangkok (Thailand). In Kedougou, the RT-RPA was operated at an ambient temperature of 38 degrees C with auxiliary electricity tapped from a motor vehicle and yielded a clinical sensitivity and specificity of 98% (n=31) and 100% (n=23), respectively. While in the field trial in Bangkok, the clinical sensitivity and specificity were 72% (n=90) and 100% (n=41), respectively. Conclusions/Significance During the first 5 days of infection, the developed DENV1-4 RT-RPA assays constitute a suitable accurate and rapid assay for DENV diagnosis. Moreover, the use of a portable fluorescence-reading device broadens its application potential to the point-of-care for outbreak investigations."],["dc.identifier.doi","10.1371/journal.pone.0129682"],["dc.identifier.isi","000356329900091"],["dc.identifier.pmid","26075598"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11961"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36842"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Recombinase Polymerase Amplification Assay for Rapid Diagnostics of Dengue Infection"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","59"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Acta physiologica"],["dc.bibliographiccitation.lastpage","73"],["dc.bibliographiccitation.volume","221"],["dc.contributor.author","Wottawa, M."],["dc.contributor.author","Naas, S."],["dc.contributor.author","Böttger, J."],["dc.contributor.author","van Belle, G. J."],["dc.contributor.author","Möbius, W."],["dc.contributor.author","Revelo, Natalia H."],["dc.contributor.author","Heidenreich, D."],["dc.contributor.author","von Ahlen, M."],["dc.contributor.author","Zieseniss, A."],["dc.contributor.author","Kröhnert, K."],["dc.contributor.author","Lutz, S."],["dc.contributor.author","Lenz, C."],["dc.contributor.author","Urlaub, H."],["dc.contributor.author","Rizzoli, S. O."],["dc.contributor.author","Katschinski, D. M."],["dc.date.accessioned","2018-01-09T16:05:27Z"],["dc.date.available","2018-01-09T16:05:27Z"],["dc.date.issued","2017"],["dc.description.abstract","Traffic between the plasma membrane and the endomembrane compartments is an essential feature of eukaryotic cells. The secretory pathway sends cargoes from biosynthetic compartments to the plasma membrane. This is counterbalanced by a retrograde endocytic route and is essential for cell homoeostasis. Cells need to adapt rapidly to environmental challenges such as the reduction of pO2 which, however, has not been analysed in relation to membrane trafficking in detail. Therefore, we determined changes in the plasma membrane trafficking in normoxia, hypoxia, and after reoxygenation."],["dc.identifier.doi","10.1111/apha.12859"],["dc.identifier.pmid","28218996"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11620"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1748-1716"],["dc.title","Hypoxia-stimulated membrane trafficking requires T-plastin"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","International Journal of Infectious Diseases"],["dc.bibliographiccitation.volume","21"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.contributor.author","Patel, Pranav"],["dc.contributor.author","Faye, O."],["dc.contributor.author","Heidenreich, D."],["dc.contributor.author","Sall, Amadou Alpha"],["dc.contributor.author","Hufert, Frank T."],["dc.contributor.author","Weidmann, Manfred"],["dc.date.accessioned","2018-11-07T09:42:19Z"],["dc.date.available","2018-11-07T09:42:19Z"],["dc.date.issued","2014"],["dc.format.extent","21"],["dc.identifier.doi","10.1016/j.ijid.2014.03.453"],["dc.identifier.isi","000209704000042"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/33929"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Sci Ltd"],["dc.publisher.place","Oxford"],["dc.relation.issn","1878-3511"],["dc.relation.issn","1201-9712"],["dc.title","Detection of dengue virus in ten minutes using reverse transcription recombinase polymerase amplification assay"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1110"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Journal of Clinical Microbiology"],["dc.bibliographiccitation.lastpage","1117"],["dc.bibliographiccitation.volume","51"],["dc.contributor.author","Euler, Milena"],["dc.contributor.author","Wang, Y."],["dc.contributor.author","Heidenreich, Doris"],["dc.contributor.author","Patel, Pranav"],["dc.contributor.author","Strohmeier, Oliver"],["dc.contributor.author","Hakenberg, Sydney"],["dc.contributor.author","Niedrig, Matthias"],["dc.contributor.author","Hufert, Frank T."],["dc.contributor.author","Weidmann, Manfred"],["dc.date.accessioned","2018-11-07T09:26:51Z"],["dc.date.available","2018-11-07T09:26:51Z"],["dc.date.issued","2013"],["dc.description.abstract","Syndromic panels for infectious disease have been suggested to be of value in point-of-care diagnostics for developing countries and for biodefense. To test the performance of isothermal recombinase polymerase amplification (RPA) assays, we developed a panel of 10 RPAs for biothreat agents. The panel included RPAs for Francisella tularensis, Yersinia pestis, Bacillus anthracis, variola virus, and reverse transcriptase RPA (RT-RPA) assays for Rift Valley fever virus, Ebola virus, Sudan virus, and Marburg virus. Their analytical sensitivities ranged from 16 to 21 molecules detected (probit analysis) for the majority of RPA and RT-RPA assays. A magnetic bead-based total nucleic acid extraction method was combined with the RPAs and tested using inactivated whole organisms spiked into plasma. The RPA showed comparable sensitivities to real-time RCR assays in these extracts. The run times of the assays at 42 degrees C ranged from 6 to 10 min, and they showed no cross-detection of any of the target genomes of the panel nor of the human genome. The RPAs therefore seem suitable for the implementation of syndromic panels onto microfluidic platforms."],["dc.identifier.doi","10.1128/JCM.02704-12"],["dc.identifier.isi","000316220400009"],["dc.identifier.pmid","23345286"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/30393"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Microbiology"],["dc.relation.issn","1098-660X"],["dc.relation.issn","0095-1137"],["dc.title","Development of a Panel of Recombinase Polymerase Amplification Assays for Detection of Biothreat Agents"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Oncology Research and Treatment"],["dc.bibliographiccitation.volume","38"],["dc.contributor.author","Pfeifer, H."],["dc.contributor.author","Cayuela, J.-M."],["dc.contributor.author","Spiekermann, Karsten"],["dc.contributor.author","Beck, J."],["dc.contributor.author","Jung, Werner"],["dc.contributor.author","Viardot, Andreas"],["dc.contributor.author","Schaefer-Eckhart, K."],["dc.contributor.author","Reichle, Albrecht"],["dc.contributor.author","Maury, S."],["dc.contributor.author","Schmitz, Norbert"],["dc.contributor.author","Heidenreich, D."],["dc.contributor.author","Panse, Jens"],["dc.contributor.author","Junghanss, Christian"],["dc.contributor.author","Raffoux, E."],["dc.contributor.author","Suarez, F."],["dc.contributor.author","Guillerm, G."],["dc.contributor.author","Alexis, M."],["dc.contributor.author","Lissandre, S."],["dc.contributor.author","Huguet, F."],["dc.contributor.author","Isnard, F."],["dc.contributor.author","Lepetre, S."],["dc.contributor.author","Escofffre-Barbe, M."],["dc.contributor.author","Ribera, J.-M."],["dc.contributor.author","Goekbuget, Nicola"],["dc.contributor.author","Dombret, H."],["dc.contributor.author","Hoelzer, D."],["dc.contributor.author","Rousselot, P."],["dc.contributor.author","Ottmann, Oliver G."],["dc.date.accessioned","2018-11-07T09:50:37Z"],["dc.date.available","2018-11-07T09:50:37Z"],["dc.date.issued","2015"],["dc.format.extent","88"],["dc.identifier.isi","000364268800207"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/35740"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Karger"],["dc.publisher.place","Basel"],["dc.relation.issn","2296-5262"],["dc.relation.issn","2296-5270"],["dc.title","European working group for adult ALL phase II trial of nilotinib in combination with chemotherapy for first-line treatment in elderly patients with de novo Philadelphiachromosome positive acute lymphoblastic leukemia (EWALL-PH-02)"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]