Kuhlmann, Jan W.

[["dc.bibliographiccitation.firstpage","8186"],["dc.bibliographiccitation.issue","27"],["dc.bibliographiccitation.journal","Langmuir"],["dc.bibliographiccitation.lastpage","8192"],["dc.bibliographiccitation.volume","30"],["dc.contributor.author","Kuhlmann, Jan W."],["dc.contributor.author","Mey, Ingo P."],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2017-09-07T11:45:42Z"],["dc.date.available","2017-09-07T11:45:42Z"],["dc.date.issued","2014"],["dc.description.abstract","The plasma membrane of animal cells is attached to the cytoskeleton, which significantly contributes to the lateral tension of the membrane. Lateral membrane tension has been shown to be an important physical regulator of cellular processes such as cell motility and morphology as well as exo- and endocytosis. Here, we report on lipid bilayers spanning highly ordered pore arrays, where we can control the lateral membrane tension by chemically varying the surface functionalization of the porous substrate. Surface functionalization was achieved by a gold coating on top of the pore rims of the hexagonal array of pores in silicon nitride substrates with pore radii of 600 nm followed by subsequent incubation with various n-propanolic mixtures of 6-mercapto-1-hexanol (6MH) and O-cholesteryl N-(8'-mercapto-3',6'-dioxaoctyl)carbamate (CPEO3). Pore-spanning membranes composed of 1,2-diphytanoyl-sn-glycero-3-phosphocholine were prepared by spreading giant unilarnellar vesicles on these functionalized porous silicon nitride substrates. Different mixtures of 6MH and CPEO3 provided self-assembled monolayers (SAMs) with different compositions as analyzed by contact angle and PM-IRRAS measurements. Site specific force-indentation experiments on the pore-spanning membranes attached to the different SAMs revealed a clear dependence of the amount of CPEO3 in the monolayer on the lateral membrane tension. While bilayers on pure 6MH monolayers show an average lateral membrane tension of 1.4 mN m(-1), a mixed monolayer of CPEO3 and 6MH obtained from a solution with 9.1 mol % CPEO3 exhibits a lateral tension of 5.0 mN m(-1). From contact angle and PM-IRRAS results, the mole fraction of CPEO3 in solution can be roughly translated into a CPEO3 surface concentration of 40 mol %. Our results clearly demonstrate that the free energy difference between the supported and freestanding part of the membrane depends on the chemical composition of the SAM, which controls the lateral membrane tension."],["dc.identifier.doi","10.1021/la5019086"],["dc.identifier.gro","3142088"],["dc.identifier.isi","000339229000031"],["dc.identifier.pmid","24950370"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/4411"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: DFG [SFB 803]"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0743-7463"],["dc.title","Modulating the Lateral Tension of Solvent-Free Pore-Spanning Membranes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","566"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Biochimica et Biophysica Acta (BBA) - Biomembranes"],["dc.bibliographiccitation.lastpage","578"],["dc.bibliographiccitation.volume","1860"],["dc.contributor.author","Xu, Yihui"],["dc.contributor.author","Kuhlmann, Jan"],["dc.contributor.author","Brennich, Martha"],["dc.contributor.author","Komorowski, Karlo"],["dc.contributor.author","Jahn, Reinhard"],["dc.contributor.author","Steinem, Claudia"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2018-01-17T13:00:13Z"],["dc.date.available","2018-01-17T13:00:13Z"],["dc.date.issued","2018"],["dc.description.abstract","SNAREs are known as an important family of proteins mediating vesicle fusion. For various biophysical studies, they have been reconstituted into supported single bilayers via proteoliposome adsorption and rupture. In this study we extended this method to the reconstitution of SNAREs into supported multilamellar lipid membranes, i.e. oriented multibilayer stacks, as an ideal model system for X-ray structure analysis (X-ray reflectivity and diffraction). The reconstitution was implemented through a pathway of proteomicelle, proteoliposome and multibilayer. To monitor the structural evolution in each step, we used small-angle X-ray scattering for the proteomicelles and proteoliposomes, followed by X-ray reflectivity and grazing-incidence small-angle scattering for the multibilayers. Results show that SNAREs can be successfully reconstituted into supported multibilayers, with high enough orientational alignment for the application of surface sensitive X-ray characterizations. Based on this protocol, we then investigated the effect of SNAREs on the structure and phase diagram of the lipid membranes. Beyond this application, this reconstitution protocol could also be useful for X-ray analysis of many further membrane proteins."],["dc.identifier.doi","10.1016/j.bbamem.2017.10.023"],["dc.identifier.pmid","29106973"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11697"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray scattering"],["dc.subject.gro","membrane biophysics"],["dc.title","Reconstitution of SNARE proteins into solid-supported lipid bilayer stacks and X-ray structure analysis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1914"],["dc.bibliographiccitation.issue","22"],["dc.bibliographiccitation.journal","Neurology"],["dc.bibliographiccitation.lastpage","1921"],["dc.bibliographiccitation.volume","72"],["dc.contributor.author","Goldschmidt, Thomas"],["dc.contributor.author","Antel, Jack P."],["dc.contributor.author","Koenig, Fatima Barbara"],["dc.contributor.author","Brueck, Wolfgang"],["dc.contributor.author","Kuhlmann, T."],["dc.date.accessioned","2018-11-07T08:28:49Z"],["dc.date.available","2018-11-07T08:28:49Z"],["dc.date.issued","2009"],["dc.description.abstract","Objective: To analyze and compare the extent of remyelination in lesions from patients with multiple sclerosis (MS) who have a short (early MS lesions) or a long (chronic MS lesions) disease duration and to determine the influence of anatomic localization on the extent of remyelination. In early MS lesions, remyelination has been described as a relatively frequent event, in contrast to chronic MS lesions, where remyelination is absent or limited to the lesion border in the majority of lesions. However, no studies have been published that have quantified and compared the extent of remyelination in early and chronic MS lesions. Methods: We analyzed the occurrence of remyelination in 52 biopsies from 51 patients (early MS) and in 174 lesions from 36 autopsy cases (chronic MS) by immunohistochemistry for myelin proteins, and correlated our findings with anatomic localization, sex, age, and disease duration. Results: Significantly more lesions were remyelinated in early than in chronicMS (80.7% vs 60%). In chronic MS, subcortical lesions showed more extensive remyelination than periventricular lesions. The majority of cerebellar lesions were completely demyelinated. Conclusion: In summary, our data demonstrate that remyelination is a frequent event in early multiple sclerosis lesions. Furthermore, the anatomic localization of a lesion might influence the extent of remyelination. Neurology (R) 2009; 72: 1914-1921"],["dc.identifier.doi","10.1212/WNL.0b013e3181a8260a"],["dc.identifier.isi","000266524600007"],["dc.identifier.pmid","19487649"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/16511"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.relation.issn","0028-3878"],["dc.title","Remyelination capacity of the MS brain decreases with disease chronicity"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2573"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","2574"],["dc.bibliographiccitation.volume","112"],["dc.contributor.author","Kuhlmann, Jan W."],["dc.contributor.author","Junius, Meike"],["dc.contributor.author","Diederichsen, Ulf"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2018-01-17T13:03:59Z"],["dc.date.available","2018-01-17T13:03:59Z"],["dc.date.issued","2017"],["dc.description.abstract","In vitro single-vesicle fusion assays are important tools to analyze the details of SNARE-mediated fusion processes. In this study, we employed planar pore-spanning membranes (PSMs) prepared on porous silicon substrates with large pore diameters of 5 μm, allowing us to compare the process of vesicle docking and fusion on the supported parts of the PSMs (s-PSMs) with that on the freestanding membrane parts (f-PSM) under the exact same experimental conditions. The PSMs harbor the t-SNARE ΔN49-complex to investigate the dynamics and fusogenicity of single large unilamellar vesicles doped with the v-SNARE synaptobrevin 2 by means of spinning-disc confocal microscopy with a time resolution of 10 ms. Our results demonstrate that vesicles docked to the s-PSM were fully immobile, whereas those docked to the f-PSM were mobile with a mean diffusion coefficient of 0.42 μm2/s. Despite the different dynamics of the vesicles on the two membrane types, similar fusion kinetics were observed, giving rise to a common fusion mechanism. Further investigations of individual lipid mixing events on the s-PSMs revealed semi-stable post-fusion structures."],["dc.identifier.doi","10.1016/j.bpj.2017.04.032"],["dc.identifier.pmid","28591607"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11702"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1542-0086"],["dc.title","SNARE-Mediated Single-Vesicle Fusion Events with Supported and Freestanding Lipid Membranes"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","European Biophysics Journal"],["dc.bibliographiccitation.volume","42"],["dc.contributor.author","Kuhlmann, Jan"],["dc.contributor.author","Mey, Ingo"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2018-11-07T09:22:33Z"],["dc.date.available","2018-11-07T09:22:33Z"],["dc.date.issued","2013"],["dc.format.extent","S102"],["dc.identifier.isi","000330215300254"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29371"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","New york"],["dc.relation.eventlocation","Lisbon, PORTUGAL"],["dc.relation.issn","1432-1017"],["dc.relation.issn","0175-7571"],["dc.title","Modulating tension in pore spanning membranes"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","520a"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","110"],["dc.contributor.author","Hubrich, Raphael"],["dc.contributor.author","Kuhlmann, Jan"],["dc.contributor.author","Schwenen, Lando L.G."],["dc.contributor.author","Milovanovic, Dragomir"],["dc.contributor.author","Jahn, Reinhard"],["dc.contributor.author","Geil, Burkhard"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2020-12-10T14:22:42Z"],["dc.date.available","2020-12-10T14:22:42Z"],["dc.date.issued","2016"],["dc.identifier.doi","10.1016/j.bpj.2015.11.2779"],["dc.identifier.isi","000375143000026"],["dc.identifier.issn","0006-3495"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71699"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.publisher.place","Cambridge"],["dc.relation.eventlocation","Los Angeles, CA"],["dc.title","Planar Pore-Spanning Membranes: A Platform to Study Snare-Mediated Fusion Processes"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]