Lüder, Carsten Günter Kurt

[["dc.bibliographiccitation.firstpage","105"],["dc.bibliographiccitation.journal","International Journal of Medical Microbiology"],["dc.bibliographiccitation.lastpage","106"],["dc.bibliographiccitation.volume","293"],["dc.contributor.author","Keller, Peter"],["dc.contributor.author","Goebel, S."],["dc.contributor.author","Fischer, S. F."],["dc.contributor.author","Hacker, G."],["dc.contributor.author","Gross, U."],["dc.contributor.author","Luder, C. G. K."],["dc.date.accessioned","2018-11-07T10:50:40Z"],["dc.date.available","2018-11-07T10:50:40Z"],["dc.date.issued","2004"],["dc.identifier.isi","000222589900164"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/48707"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Urban & Fischer Verlag"],["dc.publisher.place","Jena"],["dc.relation.conference","21st Congress of the German-Society-of-Parasitology"],["dc.relation.eventlocation","Univ Wurzburg, Wurzburg, GERMANY"],["dc.relation.issn","1438-4221"],["dc.title","Inhibition of caspase activity by Toxoplasma gondii in a cell-free system indicates novel mechanisms of interference with host cell apoptosis"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","664"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","APOPTOSIS"],["dc.bibliographiccitation.lastpage","680"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Nyoman, Ayu Dewi Ni"],["dc.contributor.author","Lueder, Carsten Guenter Kurt"],["dc.date.accessioned","2018-11-07T09:24:30Z"],["dc.date.available","2018-11-07T09:24:30Z"],["dc.date.issued","2013"],["dc.description.abstract","Ancient pathways of an apoptosis-like cell death have been identified in unicellular eukaryotes including protozoan parasites. Here, we examined programmed cell death in the apicomplexan Toxoplasma gondii which is a common intracellular pathogen of humans and warm-blooded animals. Treatment of extracellular T. gondii with various pro-apoptotic stimuli significantly induced DNA strand breaks as revealed by TUNEL and flow cytometry. Using staurosporine or miltefosine as pro-apoptotic stimuli, parasites also presented a reduced cell size, i.e. pyknosis and externalized phosphatidylserine while the plasma membrane remained intact. Importantly, staurosporine also induced DNA strand breaks in intracellular T. gondii. Data mining of the Toxoplasma genome resource identified 17 putative cell death-associated genes encoding proteases, a nuclease and several apoptosis regulators. Staurosporine-treated parasites but not controls strongly up-regulated several of these genes in a time-dependent fashion with a putative PDCD2 protein being more than 100-fold up-regulated. However, the mitochondrial membrane potential (Delta I (m)) remained intact and caspase-like activity increased only slightly during staurosporine-triggered cell death. As compared to staurosporine, the transcriptional response of parasites to miltefosine was more restricted but PDCD2 was again strongly induced. Furthermore, T. gondii lost their Delta I (m) and rapidly presented strong caspase-like activity during miltefosine treatment. Consequently, protease inhibitors abrogated miltefosine-induced but not staurosporine-induced Toxoplasma cell death. Finally, toxoplasmacidal drugs triggered DNA strand breaks in extracellular T. gondii. Interestingly, clindamycin also induced markers of an apoptosis-like cell death in intracellular parasites. Together, the data indicate that T. gondii possesses ancient apoptosis-like cell death machinery which can be triggered by chemotherapeutic agents."],["dc.description.sponsorship","COST action [BM0802]; German Academic Exchange Service (DAAD)"],["dc.identifier.doi","10.1007/s10495-013-0832-8"],["dc.identifier.isi","000318299300002"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29839"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1360-8185"],["dc.title","Apoptosis-like cell death pathways in the unicellular parasite Toxoplasma gondii following treatment with apoptosis inducers and chemotherapeutic agents: a proof-of-concept study"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","480"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Trends in Parasitology"],["dc.bibliographiccitation.lastpage","486"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Luder, C. G. K."],["dc.contributor.author","Gross, U."],["dc.contributor.author","Lopes, M. F."],["dc.date.accessioned","2018-11-07T08:38:10Z"],["dc.date.available","2018-11-07T08:38:10Z"],["dc.date.issued","2001"],["dc.description.abstract","Programmed cell death (apoptosis) is an important regulator of the host's response during infection with a variety of intracellular protozoan parasites. Parasitic pathogens have evolved diverse strategies to induce or inhibit host-cell apoptosis, thereby modulating the host's immune response, aiding dissemination within the host or facilitating intracellular survival. Here, we review the molecular and cell-biological mechanisms of the pathogen-induced modulation of host-cell apoptosis and its effects on the parasite-host interaction and the pathogenesis of parasitic diseases. We also discuss the previously unrecognized phenomenon of apoptotic cell death in (unicellular) protozoan parasites and its potential implications."],["dc.identifier.doi","10.1016/S1471-4922(01)02016-5"],["dc.identifier.isi","000171456700010"],["dc.identifier.pmid","11587962"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/18705"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Sci Ltd"],["dc.relation.issn","1471-4922"],["dc.title","Intracellular protozoan parasites and apoptosis: diverse strategies to modulate parasite-host interactions"],["dc.type","review"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","International Journal of Medical Microbiology"],["dc.bibliographiccitation.volume","297"],["dc.contributor.author","da Silva, Marialice da Fonseca Ferreira"],["dc.contributor.author","Barbosa, Helene Santos"],["dc.contributor.author","Gross, U."],["dc.contributor.author","Lueder, Carsten Guenter Kurt"],["dc.date.accessioned","2018-11-07T10:59:03Z"],["dc.date.available","2018-11-07T10:59:03Z"],["dc.date.issued","2007"],["dc.format.extent","23"],["dc.identifier.isi","000250104800053"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/50607"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Gmbh, Urban & Fischer Verlag"],["dc.publisher.place","Jena"],["dc.relation.conference","59th Annual Meeting of the Deutschen-Gesellschaft-fur-Hygiene-und-Mikrobiologie"],["dc.relation.eventlocation","Gottingen, GERMANY"],["dc.relation.issn","1438-4221"],["dc.title","Toxoplasma gondii in skeletal muscle cells in vitro: spontaneous differentiation from the tachyzoite to the bradyzoite stage"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","235"],["dc.bibliographiccitation.journal","Frontiers in Cellular and Infection Microbiology"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Ehmen, Hauke G."],["dc.contributor.author","Lüder, Carsten G. K."],["dc.date.accessioned","2019-07-09T11:51:58Z"],["dc.date.available","2019-07-09T11:51:58Z"],["dc.date.issued","2019"],["dc.description.abstract","Toxoplasma gondii is a prevalent parasite of mammals and birds including up to 30% of humans world-wide. Primary infection of immunocompetent hosts leads to a robust cell-mediated immune response, which controls but does not clear the infection, thus enabling long-term parasite persistence in brain and muscle tissues. Chronic toxoplasmosis in mice is associated with resistance to heterologous pathogens and this has been related to increased numbers of inflammatory monocytes. Here we have analyzed whether chronic T. gondii infection impacts the subset distribution and the phenotype of peripheral human monocytes in vivo and their responses to parasite infection in vitro. CD14+ monocytes from T. gondii-seropositive blood donors expressed significantly less FcγRIII (CD16) than those from seronegative controls, but they did not show a shift in the distribution of classical, intermediate and non-classical monocyte subpopulations. Percentages of CD62L+ and CD64+ monocytes were however decreased and increased, respectively, in chronically infected individuals as compared to naïve controls. Infection of monocyte-enriched PBMCs from both seropositive and seronegative individuals with T. gondii led to an increase of CD14+CD16− classical monocytes and a decrease of CD14+CD16+ double positive monocytes. Remarkably, after in vitro parasite infection, expression of the chemokine receptor CCR2 was severely impaired in monocytes from both, individuals with chronic toxoplasmosis and seronegative controls. In contrast, only monocytes from chronically infected humans but not those from controls dose-dependently up-regulated HLA-DR, DP, DQ expression following in vitro infection. Furthermore, monocyte-enriched PBMCs from seropositive individuals up-regulated IL-12 mRNA more vigorously after in vitro infection than cells from naïve controls. Collectively, our results establish that infection of humans with T. gondii exerts long-term effects on the phenotype and responsiveness of blood monocytes. This may have important implications for innate immune responses to T. gondii and unrelated pathogens."],["dc.identifier.doi","10.3389/fcimb.2019.00235"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16249"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60052"],["dc.language.iso","en"],["dc.subject.ddc","610"],["dc.title","Long-Term Impact of Toxoplasma gondii Infection on Human Monocytes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1079"],["dc.bibliographiccitation.issue","13"],["dc.bibliographiccitation.journal","Microbes and Infection"],["dc.bibliographiccitation.lastpage","1087"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Graumann, Kristin"],["dc.contributor.author","Hippe, Diana"],["dc.contributor.author","Gross, Uwe"],["dc.contributor.author","Lueder, Carsten Guenter Kurt"],["dc.date.accessioned","2018-11-07T11:22:58Z"],["dc.date.available","2018-11-07T11:22:58Z"],["dc.date.issued","2009"],["dc.description.abstract","Programmed cell death is an essential mechanism of the host to combat infectious agents and to regulate immunity during infection Consequently, activation and deactivation of the hosts' cell death pathways by protozoan parasites play critical roles in parasite control. pathogenesis, immune evasion and parasite dissemination within the host Here, we discuss advances in the understanding of these fascinating host-parasite interactions with special emphasis on how protozoa can modulate the cell death apparatus of its host. (C) 2009 Elsevier Masson SAS All rights reserved."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft [LU777/4-1]"],["dc.identifier.doi","10.1016/j.micinf.2009.08.011"],["dc.identifier.isi","000271557300011"],["dc.identifier.pmid","19733682"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56091"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","1286-4579"],["dc.title","Mammalian apoptotic signalling pathways: multiple targets of protozoan parasites to activate or deactivate host cell death"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Cellular Microbiology"],["dc.bibliographiccitation.lastpage","17"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Swierzy, Izabela J."],["dc.contributor.author","Lueder, Carsten Guenter Kurt"],["dc.date.accessioned","2018-11-07T10:04:02Z"],["dc.date.available","2018-11-07T10:04:02Z"],["dc.date.issued","2015"],["dc.description.abstract","Toxoplasma gondii is a widespread intracellular parasite of mammals and birds and an important opportunistic pathogen of humans. Following primary infection, fast-replicating tachyzoites disseminate within the host and either are subsequently eliminated by the immune system or transform to latent bradyzoites which preferentially persist in brain and muscle tissues. The factors which determine the parasites' tissue distribution during chronic toxoplasmosis are unknown. Here we show that mouse skeletal muscle cells (SkMCs) after differentiation to mature, myosin heavy chain-positive, polynucleated myotubes, significantly restrict tachyzoite replication and facilitate expression of bradyzoite-specific antigens and tissue cyst formation. In contrast, proliferating mononuclear myoblasts and control fibroblasts enable vigorous T. gondii replication but do not sustain bradyzoite or tissue cyst formation. Bradyzoite formation correlates with upregulation of testis-specific Y-encoded-like protein-2 gene expression (Tspyl2) and p21(Waf1/Cip1) as well as downregulation of cyclin B1 and absence of DNA synthesis, i.e. a cell cycle arrest of syncytial myotubes. Following infection with T.gondii, myotubes but not myoblasts or fibroblasts further upregulate the negative cell cycle regulator Tspyl2. Importantly, RNA interference-mediated knock-down of Tspyl2 abrogates differentiation of SkMCs to myotubes and enables T. gondii to replicate vigorously but abolishes bradyzoite-specific gene expression and tissue cyst formation. Together, these data indicate that Tspyl2-mediated host cell cycle withdrawal is a physiological trigger of Toxoplasma stage conversion in mature SkMCs. This finding might explain the preferred distribution of T. gondii tissue cysts in vivo."],["dc.identifier.doi","10.1111/cmi.12342"],["dc.identifier.isi","346704300002"],["dc.identifier.pmid","25131712"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38606"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","1462-5822"],["dc.relation.issn","1462-5814"],["dc.title","Withdrawal of skeletal muscle cells from cell cycle progression triggers differentiation of Toxoplasma gondii towards the bradyzoite stage"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","196"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Memórias do Instituto Oswaldo Cruz"],["dc.bibliographiccitation.lastpage","200"],["dc.bibliographiccitation.volume","104"],["dc.contributor.author","Ferreira-da-Silva, Marialice da Fonseca"],["dc.contributor.author","Rodrigues, Renata Mendonca"],["dc.contributor.author","de Andrade, Elisabete Ferreira"],["dc.contributor.author","de Carvalho, Lais"],["dc.contributor.author","Gross, Uwe"],["dc.contributor.author","Lueder, Carsten Guenter Kurt"],["dc.contributor.author","Barbosa, Helene Santos"],["dc.date.accessioned","2018-11-07T08:32:21Z"],["dc.date.available","2018-11-07T08:32:21Z"],["dc.date.issued","2009"],["dc.description.abstract","Although the predilection for Toxoplasma gondii to form cysts in the nervous system and skeletal and heart muscles has been described for more than fifty years, skeletal muscle cells (SkMCs) have not been explored as a host cell type to study the Toxoplasma-host cell interaction and investigate the intracellular development of the parasite. Morphological aspects of the initial events in the Toxoplasma-SkMC interaction were analysed and suggest that there are different processes of protozoan adhesion and invasion and of the subsequent fate of the parasite inside the parasitophorous vacuole (PV). Using scanning electron microscopy, Toxoplasma tachyzoites from the mouse-virulent RH strain were found to be attached to SkMCs by the anterior or posterior region of the body, with or without expansion of the SkMC membrane. This suggests that different types of parasite internalization occurred. Asynchronous multiplication and differentiation of T. gondii were observed. Importantly, intracellular parasites were seen to display high amounts of amylopectin granules in their cytoplasm, indicating that tachyzoites of the RH strain were able to differentiate spontaneously into bradyzoites in SkMCs. This stage conversion occurred in approximately 3% of the PVs. This is particularly intriguing as tachyzoites of virulent Toxoplasma strains are not thought to be prone to cyst formation. We discuss whether biological differences in host cells are crucial to Toxoplasma stage conversion and suggest that important questions concerning the host cell type and its relevance in Toxoplasma differentiation are still unanswered."],["dc.identifier.isi","000267051500012"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17321"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Fundaco Oswaldo Cruz"],["dc.publisher.place","Rio de janeiro, rj"],["dc.relation.conference","Meeting on Toxoplasma Centennial Congress from Discovery to Public Health Management"],["dc.relation.eventlocation","Buzios, BRAZIL"],["dc.relation.issn","0074-0276"],["dc.title","Spontaneous stage differentiation of mouse-virulent Toxoplasma gondii RH parasites in skeletal muscle cells: an ultrastructural evaluation"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","119"],["dc.bibliographiccitation.journal","International Journal of Medical Microbiology"],["dc.bibliographiccitation.lastpage","120"],["dc.bibliographiccitation.volume","294"],["dc.contributor.author","Keller, Peter"],["dc.contributor.author","Goebel, S."],["dc.contributor.author","Fischer, S. F."],["dc.contributor.author","Hacker, G."],["dc.contributor.author","Gross, U."],["dc.contributor.author","Luder, C. G. K."],["dc.date.accessioned","2018-11-07T10:45:58Z"],["dc.date.available","2018-11-07T10:45:58Z"],["dc.date.issued","2004"],["dc.identifier.isi","000224456000083"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/47630"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Urban & Fischer Verlag"],["dc.publisher.place","Jena"],["dc.relation.conference","Annual Meeting of the DGHM"],["dc.relation.eventlocation","Munster, GERMANY"],["dc.relation.issn","1438-4221"],["dc.title","Excretory-secretory antigens of Toxoplasma gondii inhibit caspase 3 activity in a cell-free system: possible role in interference with host cell apoptosis"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","International Journal of Medical Microbiology"],["dc.bibliographiccitation.volume","294"],["dc.contributor.author","Lang, C."],["dc.contributor.author","Algner, M."],["dc.contributor.author","Gross, U."],["dc.contributor.author","Luder, C. G. K."],["dc.date.accessioned","2018-11-07T10:45:58Z"],["dc.date.available","2018-11-07T10:45:58Z"],["dc.date.issued","2004"],["dc.format.extent","120"],["dc.identifier.isi","000224456000084"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/47632"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Urban & Fischer Verlag"],["dc.publisher.place","Jena"],["dc.relation.conference","Annual Meeting of the DGHM"],["dc.relation.eventlocation","Munster, GERMANY"],["dc.relation.issn","1438-4221"],["dc.title","Differential effects of Toxoplasma gondii and parasite lysate on GAS-containing promoters driving IFN-gamma-responsive genes"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]