Fischer, Charlotte Viola

[["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Investigative Ophthalmology & Visual Science"],["dc.bibliographiccitation.volume","57"],["dc.contributor.author","van Oterendorp, Christian"],["dc.contributor.author","Mans, Viktoria"],["dc.contributor.author","Fischer, Charlotte"],["dc.contributor.author","Khattab, Mohammed"],["dc.contributor.author","Wecker, Thomas"],["dc.date.accessioned","2018-11-07T10:08:43Z"],["dc.date.available","2018-11-07T10:08:43Z"],["dc.date.issued","2016"],["dc.identifier.isi","000394174001307"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/39521"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Assoc Research Vision Ophthalmology Inc"],["dc.publisher.place","Rockville"],["dc.relation.conference","Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO)"],["dc.relation.eventlocation","Seattle, WA"],["dc.relation.issn","1552-5783"],["dc.relation.issn","0146-0404"],["dc.title","12% fat milk as OCT contrast agent for ex vivo imaging."],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","397"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Investigative Ophthalmology & Visual Science"],["dc.bibliographiccitation.lastpage","406"],["dc.bibliographiccitation.volume","60"],["dc.contributor.author","Gottschalk, Hanna M."],["dc.contributor.author","Wecker, Thomas"],["dc.contributor.author","Khattab, Mohammed H."],["dc.contributor.author","Fischer, Charlotte V."],["dc.contributor.author","Callizo, Josep"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.author","Lubjuhn, Roswitha"],["dc.contributor.author","Russmann, Christoph"],["dc.contributor.author","Hoerauf, Hans"],["dc.contributor.author","van Oterendorp, Christian"],["dc.date.accessioned","2019-07-09T11:50:10Z"],["dc.date.available","2019-07-09T11:50:10Z"],["dc.date.issued","2019"],["dc.description.abstract","Purpose: Contrast agents applicable for optical coherence tomography (OCT) imaging are rare. The intrascleral aqueous drainage system would be a potential application for a contrast agent, because the aqueous veins are of small diameter and located deep inside the highly scattering sclera. We tested lipid emulsions (LEs) as candidate OCT contrast agents in vitro and ex vivo, including milk and the anesthetic substance Propofol. Methods: Commercial OCT and OCT angiography (OCTA) devices were used. Maximum reflectivity and signal transmission of LE were determined in tube phantoms. Absorption spectra and light scattering was analyzed. The anterior chamber of enucleated porcine eyes was perfused with LEs, and OCTA imaging of the LEs drained via the aqueous outflow tract was performed. Results: All LEs showed a significantly higher reflectivity than water (P < 0.001). Higher milk lipid content was positively correlated with maximum reflectivity and negatively with signal transmission. Propofol exhibited the best overall performance. Due to a high degree of signal fluctuation, OCTA could be applied for detection of LE. Compared with blood, the OCTA signal of Propofol was significantly stronger (P = 0.001). As a proof of concept, time-resolved aqueous angiography of porcine eyes was performed. The three-dimensional (3D) structure and dynamics of the aqueous outflow were significantly different from humans. Conclusions: LEs induced a strong signal in OCT and OCTA. LE-based OCTA allowed the ability to obtain time-resolved 3D datasets of aqueous outflow. Possible interactions of LE with inner eye's structures need to be further investigated before in vivo application."],["dc.identifier.doi","10.1167/iovs.18-25223"],["dc.identifier.pmid","30682210"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15874"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59715"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1552-5783"],["dc.rights","CC BY-NC-ND 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-nd/4.0"],["dc.subject.ddc","610"],["dc.title","Lipid Emulsion-Based OCT Angiography for Ex Vivo Imaging of the Aqueous Outflow Tract."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","663"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Der Ophthalmologe"],["dc.bibliographiccitation.lastpage","668"],["dc.bibliographiccitation.volume","115"],["dc.contributor.author","Fischer, C. V."],["dc.contributor.author","Kulanga, M."],["dc.contributor.author","Hoerauf, H."],["dc.date.accessioned","2020-12-10T14:10:15Z"],["dc.date.available","2020-12-10T14:10:15Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1007/s00347-017-0559-y"],["dc.identifier.eissn","1433-0423"],["dc.identifier.issn","0941-293X"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/70699"],["dc.language.iso","de"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation.haserratum","/handle/2/70700"],["dc.title","Trends in der Ablatiochirurgie"],["dc.title.alternative","Trends in retinal detachment surgery. What has changed compared to 2001?"],["dc.title.subtitle","Was hat sich im Vergleich zu 2001 geändert?"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0208980"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","PLOS ONE"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Kowallick, Antonia"],["dc.contributor.author","Fischer, Charlotte Viola"],["dc.contributor.author","Hoerauf, Hans"],["dc.date.accessioned","2019-07-09T11:50:00Z"],["dc.date.available","2019-07-09T11:50:00Z"],["dc.date.issued","2018"],["dc.description.abstract","PURPOSE: To investigate the feasibility and diagnostic benefit of routinely performed preoperative macular spectral-domain (SD-) optical coherence tomography (OCT) for the detection of macular pathology in patients with normal biomicroscopic funduscopy prior to cataract surgery. METHODS: Prospective, single center study. A total of 162 eyes of 123 consecutive patients referred for cataract surgery with a visual acuity better than 20/100, absence of macular symptoms such as metamorphopsia, no history of previous intravitreal procedures and a normal funduscopic appearance on biomicroscopy underwent an additional SD-OCT-examination prior to cataract surgery. OCT-scans were classified in three categories: normal, degenerative vitreous changes without impact on visual outcome or pathological with potential impact on visual outcome. RESULTS: 80 eyes (49.38%) showed normal OCT-scans. 69 eyes (42.59%) were classified as degenerative vitreous changes without impact on visual outcome and 20 eyes (12.35%) as pathological with potential impact on visual outcome. The indication of cataract surgery or the therapeutic strategy remained unchanged in all patients. In patients with pathological alterations further follow-up examinations were recommended. CONCLUSIONS: Routine SD-OCT-imaging of the macular region in patients prior to cataract surgery was feasible to detect macular pathologies in a considerable number of patients, which remained undiagnosed on biomicroscopic funduscopy. Although OCT-findings did not impact therapeutic strategy in this study, preoperative judgement of the expected visual outcome and patient´s informed consent can improve."],["dc.identifier.doi","10.1371/journal.pone.0208980"],["dc.identifier.pmid","30533037"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15831"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59676"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","610"],["dc.title","Optical coherence tomography findings in patients prior to cataract surgery regarded as unremarkable with ophthalmoscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4970"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Investigative Opthalmology & Visual Science"],["dc.bibliographiccitation.volume","57"],["dc.contributor.author","Fischer, Charlotte V."],["dc.contributor.author","Mans, Viktoria"],["dc.contributor.author","Horn, Maren"],["dc.contributor.author","Naxer, Sabine"],["dc.contributor.author","Klettner, Alexa"],["dc.contributor.author","van Oterendorp, Christian"],["dc.date.accessioned","2019-07-09T11:43:03Z"],["dc.date.available","2019-07-09T11:43:03Z"],["dc.date.issued","2016"],["dc.description.abstract","PURPOSE. Vascular endothelial growth factor–signaling in human tenon fibroblasts (hTFs) has recently become a target for antifibrotic treatment in glaucoma filtration surgery. The anti- VEGF antibody bevacizumab (BVC) has been shown to increase filtration bleb size. Given the relatively high concentration of BVC needed to obtain an effect, we investigated whether BVC acts through VEGF inhibition or via non–antigen-dependent ways. METHODS. Human tenon fibroblast primary cultures were obtained from strabismus surgery subjects. Under low (0.2%) and high (10%) serum conditions, cells were incubated with BVC, ranibizumab (RNB), aflibercept (AFB), or rituximab (RTX) at different concentrations. Total number of cells and number of dead or proliferating (5-bromo-2-deoxy-uridinepositive) cells were assessed after 24 hours. Concentrations of VEGF-A in cell culture media was measured with ELISA. Intracellular IgG was detected with immunostaining and Western blot analysis. RESULTS. In quiescent hTF culture (0.2% serum) the addition of 5 mg/mL BVC induced widespread cell death. Under proliferative conditions (10% serum), BVC reduced the number of proliferating cells. No such effect was observed with 2.5 mg/mL BVC or with 10 mg/mL AFB or 2.5 mg/mL RNB, although they were equally effective in binding free VEGF-A in the culture media. Instead, the CD20 antibody RTX, which did not bind VEGF, induced hTF death and inhibited proliferation in a BVC-comparable fashion. Bevacizumab, AFB, and RTX were detected intracellularly in a concentration-dependent manner. CONCLUSIONS. The cell death–inducing and antiproliferative effect of 5 mg/mL BVC appeared not to depend on VEGF inhibition. Our data question a direct role of VEGF for hTF survival and proliferation."],["dc.identifier.doi","10.1167/iovs.16-19938"],["dc.identifier.pmid","27654424"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14091"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58812"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1552-5783"],["dc.rights","CC BY-NC-ND 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-nd/4.0"],["dc.title","The Antiproliferative Effect of Bevacizumab on Human Tenon Fibroblasts Is Not Mediated by Vascular Endothelial Growth Factor Inhibition"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","24"],["dc.bibliographiccitation.journal","BMC Neurology"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Fischer, Charlotte"],["dc.contributor.author","Kleinschnitz, Konstanze"],["dc.contributor.author","Wrede, Arne"],["dc.contributor.author","Muth, Ingrid E."],["dc.contributor.author","Kruse, Niels"],["dc.contributor.author","Nishino, Ichizo"],["dc.contributor.author","Schmidt, Jens"],["dc.date.accessioned","2018-11-07T09:27:04Z"],["dc.date.available","2018-11-07T09:27:04Z"],["dc.date.issued","2013"],["dc.description.abstract","Background: Mutations of the UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine-kinase (GNE)-gene are causally related to GNE myopathy. Yet, underlying pathomechanisms of muscle fibre damage have remained elusive. In sporadic inclusion body myositis (sIBM), the pro-inflammatory cell-stress mediators alpha B-crystallin and inducible nitric oxide synthase (iNOS) are crucial markers of the disease pathology. Methods: 10 muscle biopsies from GNE myopathy patients were analyzed for mRNA-expression of markers of cell-stress, inflammation and beta-amyloid and compared to non-myopathic controls. Using double-labeling immunohistochemistry, serial sections of skeletal muscle biopsies were stained for amyloid precursor protein (APP), major histocompatibility complex (MHC)-I, alpha B-crystallin, neural cell adhesion molecule (NCAM), interleukin (IL)-1 beta, beta-amyloid, iNOS, and phosphorylated neurofilament (P-neurofilament) as well as hematoxylin/eosin histochemistry. Corresponding areas of all biopsies with a total of 2,817 muscle fibres were quantitatively assessed for all markers. Results: mRNA-expression of APP, NCAM, iNOS, TNF-alpha and TGF-beta was higher in GNE myopathy compared to controls, yet this was not statistically significant. The mRNA-expression of APP and alpha B-crystallin significantly correlated with the expression of several pro-inflammatory and cell-stress-associated markers as NCAM, IL-1 beta, TGF-beta, CCL-3, and CCL4. By immunohistochemistry, alpha B-crystallin and iNOS were co-upregulated and the number of fibres positive for alpha B-crystallin, NCAM, MHC-I and iNOS significantly correlated with each other. A large fraction of fibres positive for alpha B-crystallin were double positive for iNOS and vice-versa. Moreover, several fibres with structural abnormalities were positive for alpha B-crystallin and iNOS. Notably, particularly normal appearing fibres displayed an overexpression of these molecules. Conclusions: The cell-stress molecules alpha B-crystallin and iNOS are overexpressed in GNE myopathy muscle and may identify early disease mechanisms. The data help to better understand the pathology of GNE myopathy."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2013"],["dc.identifier.doi","10.1186/1471-2377-13-24"],["dc.identifier.isi","000317485500001"],["dc.identifier.pmid","23496965"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/8778"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/30452"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-2377"],["dc.rights","CC BY 2.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.title","Cell stress molecules in the skeletal muscle of GNE myopathy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.journal","Journal of Ophthalmology"],["dc.bibliographiccitation.lastpage","6"],["dc.bibliographiccitation.volume","2017"],["dc.contributor.author","Fischer, Charlotte"],["dc.contributor.author","Bruggemann, Anne"],["dc.contributor.author","Hager, Annette"],["dc.contributor.author","Callizo Planas, Josep"],["dc.contributor.author","Roider, Johann"],["dc.contributor.author","Hoerauf, Hans"],["dc.date.accessioned","2019-07-09T11:43:48Z"],["dc.date.available","2019-07-09T11:43:48Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1155/2017/9120892"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14677"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58971"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Vascular Occlusions following Ocular Surgical Procedures: A Clinical Observation of Vascular Complications after Ocular Surgery"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","669"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Der Ophthalmologe"],["dc.bibliographiccitation.lastpage","669"],["dc.bibliographiccitation.volume","115"],["dc.contributor.author","Fischer, C. V."],["dc.contributor.author","Kulanga, M."],["dc.contributor.author","Hoerauf, H."],["dc.date.accessioned","2020-12-10T14:10:16Z"],["dc.date.available","2020-12-10T14:10:16Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1007/s00347-017-0621-9"],["dc.identifier.eissn","1433-0423"],["dc.identifier.issn","0941-293X"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/70700"],["dc.language.iso","de"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation.iserratumof","/handle/2/70699"],["dc.title","Correction: Trends in der Ablatiochirurgie"],["dc.title.alternative","Correction: Trends in retinal detachment surgery. What has changed compared to 2001?"],["dc.title.subtitle","Was hat sich im Vergleich zu 2001 geändert?"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","erratum_ja"],["dspace.entity.type","Publication"]]